PCR/qPCR

MIDSCI can supply almost every needed product for PCR/qPCR: tubes, plates, strips, caps, taq, Mastermixes, dNPTs, dye and probe for qPCR, sealing film and much more.  If you are looking for any of these things, we can match almost all needs to all cyclers.  If you don’t find it easily contact us at tech@midsci.com and we will find it for you. 

96 or 384 we have it.  Non-Skirted, semi-skirted or full skirted, we have it.  0.1, 0.2, or 0.5 mL we have it.  A1, A12, H12, A24, P24 cuts, we have them.  Sybr© Green or probe alternative, we have it.  

Pro Tip: Colored plastics do not have any impact on DNA amplification.  But when setting up qPCR it is often recommended to use white plastics.  This will limit or prevent fluorescence refraction.  Light refraction can minimize sensitivity and consistency. 

Pro Tip 2: If you are seeing inconsistencies in PCR/qPCR try PR1MA Pipette Tips.  This will help optimize and reduce variance in the reactions.  Simple test, see the residual liquid left in tips?  This means some of the taq, dNTPs, sample, etc is left in the tip.  PR1MA tips help reduce that significantly and improve your consistency. 

Pro Tip 3: Seeing evaporation in the outer wells of a PCR/qPCR plate?  Sometimes it’s the plate, sometimes it the seal, and sometimes it’s the cycler.  Call us we have solutions.

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PR1MA Semi-Skirted PCR Plates

  • PR1MA™ PCR Plates have uniformed wells and ultra-thin walls provide optimal heat transfer and reaction efficiency.
  • Fits most thermal cyclers.
  • High Contrast black alphanumerics for easy well identification.
  • Reclosable bags minimize contamination risk and are convenient for opening and closing.
  • For use in medical, pharmaceutical, food industry, and molecular biology.
  • Barcoded versions available upon special order. 
  • Certified free of detectable RNase, DNase, NA, PCR inhibitors, and tested pyrogen-free.
PR1MA™  Non-Skirted PCR Plates
  • PR1MA™ PCR Plates have uniformed wells and ultra-thin walls provide optimal heat transfer and reaction efficiency.
  • Fits most thermal cyclers.
  • High Contrast black alphanumerics for easy well identification.
  • Reclosable bags minimize contamination risk and are convenient for opening and closing.
  • For use in medical, pharmaceutical, food industry, and molecular biology.
Certified free of detectable RNase, DNase, NA, PCR inhibitors, and tested pyrogen-free.

PR1MA PCR Strip Tubes & Caps Sold Together

  • PR1MA™ PCR Strip Tubes and Caps are evaporation-resistant and provide easy-to-open-and-close lids that prevent sample loss.
  • The uniform wells and ultra-thin walls provide optimal heat transfer and reaction efficiency.
  • Reclosable bags minimize contamination risk and are convenient for opening and closing.
  • For use in medical, pharmaceutical, food industry, and molecular biology.
  • Certified free of detectable RNase, DNase, NA, PCR inhibitors, and tested pyrogen-free.

PR1MA PCR Strip Tubes with Attached Caps

  • PR1MA™ PCR Strip Tubes and Caps are evaporation-resistant and provide easy-to-open-and-close lids that prevent sample loss.
  • The uniform wells and ultra-thin walls provide optimal heat transfer and reaction efficiency.
  • Reclosable bags minimize contamination risk and are convenient for opening and closing.
  • For use in medical, pharmaceutical, food industry, and molecular biology.
  • Certified free of detectable RNase, DNase, NA, PCR inhibitors, and tested pyrogen-free.

PR1MA Full-Skirted PCR Plates
  • PR1MA™ PCR Plates have uniformed wells and ultra-thin walls provide optimal heat transfer and reaction efficiency.
  • Fits most thermal cyclers.
  • High Contrast black alphanumerics for easy well identification.
  • Reclosable bags minimize contamination risk and are convenient for opening and closing.
  • For use in medical, pharmaceutical, food industry, and molecular biology.
  • Barcoded versions available upon special order. 
  • Certified free of detectable RNase, DNase, NA, PCR inhibitors, and tested pyrogen-free.

PR1MA PCR Strip Tubes & Caps Sold Separately

  • PR1MA™ PCR Strip Tubes and Caps are evaporation-resistant and provide easy-to-open-and-close lids that prevent sample loss.
  • The uniform wells and ultra-thin walls provide optimal heat transfer and reaction efficiency.
  • Reclosable bags minimize contamination risk and are convenient for opening and closing.
  • For use in medical, pharmaceutical, food industry, and molecular biology.
  • Certified free of detectable RNase, DNase, NA, PCR inhibitors, and tested pyrogen-free.

PR1MA Individual PCR Tubes

  • PR1MA™ Individual PCR tubes are evaporation-resistant and provide easy-to-open-and-close lids that prevent sample loss.
  • The uniform wells and ultra-thin walls provide optimal heat transfer and reaction efficiency.
  • Reclosable bags minimize contamination risk and are convenient for opening and closing.
  • For use in medical, pharmaceutical, food industry, and molecular biology.
  • Certified free of detectable RNase, DNase, NA, PCR inhibitors, and tested pyrogen-free.

Mystaire® PCR prep stations are compact laminar flow enclosures that reduce the risk of sample contamination while performing polymerase chain reaction experiments.


Airborne contaminants are prevented from entering into the work zone through constant positive pressure HEPA filtered air. Each PCR prep station is equipped with a built-in 254 nanometer shortwave lamp to effectively decontaminate the work area between amplifications. Safety interlocks are included on all PCR prep stations to prevent operator exposure to potentially harmful UV radiation.


MY-PCR prep stations are bench-top personal clean zones allowing the operator to amplify RNA and DNA while minimizing the potential for background from airborne contaminants.


Note: MY-PCR and FE-PCR are not biological safety cabinets. They are designed to provide process protection only from potential airborne contaminants found in the laboratory space.


MY-PCR WORKSTATIONS FEATURES

  1. Class 100 vertical laminar flow air
  2. 15-Minute UV light timer
  3. Clear polycarbonate construction with chemically impervious polypropylene work surface
  4. UV shelf with integrated pipette holder
  5. Electrical cord pass-through port

Sash position switch and overlap to prevent UV light exposure

Specs

MY-PCR24

MY-PCR32

MY-PCR48

Width

24"

32"

48"

Depth

24"

Height

30"

Noise Level

<45 DBA

<49 DBA

<56 DBA

Voltage

110 V or 220 V

Power

200 Watts

400 Watts

UV Light

15 W/254 mm

15 W/254 mm (2)

Blower

Sparkless Brushless

UV Light Timer

0-15 min.

Safety Interlock

Yes

Air Velocity

60 LFM

HEPA Filters, Pre-Filters, UV bulbs, additional ports and shelves also available.

Item#:
ACRPCRHOOD4

PR1MA Sealing Mats

  • PR1MA™ Sealing Mats are made of silicone and the dimensions are 4.67 in. (L) x 2.90 in. (W).
  • Pierceable
  • Designed for an evaporation-resistant seal to minimize sample loss.
  • For use in medical, pharmaceutical, food industry, and molecular biology.
  • Certified free of detectable RNase, DNase, DNA, and PCR inhibitors.
Item#:
ASPR1MAPCRMAT

**New Name, Same Great Product!**
PR1MA Taq

  • Ideal for routine PCR applications as well as genotyping, colony PCR and fast PCR
  • Improved template affinity and solubility for higher enzyme activity and greater yields
  • Proprietary buffer optimized for a variety of assay conditions
  • Conveniently supplied as a 2X Master Mix or in a 2-tube format of polymerase and separate 5X buffer

PR1MA Taq provides superior results for routine applications. Modified to improve DNA-binding, this polymerase offers higher solubility and greater template affinity, resulting in consistently superior performance. PR1MA Taq Polymerase exhibits a 5' to 3' nuclease activity, but no 3' to 5' (proofreading) activity and works well with a wide range of DNA templates including GC-rich sequences.

The polymerase is supplied with a 5X buffer containing MgCl2 and proprietary mix of enhancers (dNTP's not included). For convenience, PR1MA Taq is also available in a ready to use 2X Master Mix - just add primers and template DNA. Our "Red Dye" Master Mix incorporates a red loading dye that allows amplified samples to be loaded directly on an agarose gel.  The red color aids in visualization during pipetting, and higher density of the buffer ensures that the samples will drop into the gel wells.



LabDoctor C2000 is now PR1MA™ PCRCENT - New name, same great product.
If you have any questions please email tech@midsci.com


 
PR1MA™ Microplate Microcentrifuge

  • Quickly spins down droplets in PCR plates (less than 20 seconds)
  • Accepts skirted, Non-Skirt, standard, and most microtiter PCR plates
  • 50% smaller than traditional centrifuges
  • Unique rotor design prevents sample spillage

The PR1MA™ Microplate Microcentrifuge is the first mini sized centrifuge with a swing out rotor for microplates. Centrifuging plates before PCR ensures all reactants are in the bottom of the wells for proper concentrations and improved yields.

The easily accessible rotor chamber includes two plate carriers that rest at a 75° angle. This allows plates to be inserted confidently without sealing tapes or caps. Upon closing the centrifuge lid, the rotor accelerates and the centrifugal force "swings" the plates into a horizontal position, concentrating any droplets on the plate walls to the well bottoms.


Specifications

Capacity

2 PCR Plates (4 if stackable)

Rotor Speed

2,100 rpm

G Force

400 x g

Rotor

Swing Out

Dimensions (W x D x H)

9.2 x 10.2 x 7.75 in. | 23 x 26 x 19.7 cm

Weight

9 lbs. | 4.1 kg

Electrical

120V or 230V, 50-60 Hz

  

Accessories 

C2000-A02 - This tube adapter for the PCRCENT Plate Centrifuge holds 96 0.2 mL tubes or 8 and 12 position PCR strips. It comes 2 per pack.

C2000-DWMP - This optional rotor for the PCRCENT Plate Centrifuge has carriers for deep well plates up to 35 mm tall.

Item#:
ASPCRCENT2

MultiGene Thermal Cycler

MIDSCI bring you the new MultiGene™ Thermal Cycler, which guarantees to produce consistent and reliable results while making gradient technology affordable. This unit is extremely simple to program, compact in design and built to perform.

Features:

  • Faster speeds - up to 30 min
  • Gradient capability
  • 6-segment block temperature control
    • Independent block temperature selection capabilities
  • Precision thermal control
  • TM calculator
  • PC Viewer
  • No condensation after overnight cooling at 4°C
  • Simple, user-friendly programming
  • 3-yr warranty

The MultiGene™ OptiMax employs a new protocol optimization process improving on older gradient features. The OptiMax utilizes Flex™ technology that effectively separates the 96 well plate into 6 - (4 x 4) temperature regions that allow you to pick the temperatures you want to test against. These regions are easily identified by the blue and black squares visible on the microplate platen. Instead of having the thermal cycler choose temperatures for you, you can choose any temperatures you want within a 24°C range.

The MultiGene™ OptiMax has a heating rate of 4°C /sec and a cooling rate of 3°C/sec. We have minimized overshooting and undershooting so that your reactions only see the temperatures they are supposed to see. This also leads to faster runs time.


New is the PC Viewer Option.

By downloading our software from our website and attaching a USB cable to your Windows PC to can view the temperature profiles real time as they are occurring to monitor your system.


Intuitive User Interface


The MultiGene™ OptiMax utilizes an intuitive user interface. This user interface is friendly to the laboratory environment and consistent with what our customers previously enjoyed in our earlier models. It can be used with lab gloves even if wet. We have improved the ramping speed and eliminated overshooting and undershooting which contributes to longer run times.


Fully Adjustable Heated Lid


The OptiMax's heated lid is fully adjustable to provide optimum pressure for use with a broad range of tubes and microplates. For optimum performance, the lid can also be programmed to hold different temperatures between 60° to 65°C and 100° to 115°C. The OptiMax lid prevents condensation and slides away and back from the user to provide full access to samples and reduce the risk of coming in contact with a hot surface.


Product Specifications:


Sample Capacity

1 x 96 well plate, 12 8 x 0.2 mL strip tubes, 96 x 0.2 mL tubes

Programmable Temperature Range

4°C to 99.9°C

Temperature Control

Calculated or block

Temperature Accuracy/Uniformity

±0.5°C/±0.5°C

Heating/cooling method

Peltier

Max. heating/cooling rate

4°C/3°C per second

Temperature range of 6-segment blocks

30°C to 99°C. Temperature of 6-segment blocks can be set independently.

Maximum Temperature difference between 6-segment blocks

24°C

6-segement temperature blocks

6 temperature blocks in 4 x 4 well format

Programmable lid temperature

60° to 65°C, 100° to 115°C

Program memory

200 complete programs

Temp. increments/decrements

Yes

User program folders

50 sets

Password protected programs

Yes

Communication

USB and RS232 ports

Dimensions (W x D x H)

9.4 x 16.5 x 9.8 inches

Weight

19.8 lbs

Electrical

120 V (TC9610) or 240 V (TC9610-230), 50/60 Hz

Item#:
ASPCRTC8
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