Mystaire® PCR Prep Stations

They are compact laminar flow enclosures that reduce the risk of sample contamination while performing polymerase chain reaction experiments.

Airborne contaminants are prevented from entering into the work zone through constant positive pressure HEPA filtered air. Each PCR prep station is equipped with a built-in 254 nanometer shortwave lamp to effectively decontaminate the work area between amplifications. Safety interlocks are included on all PCR prep stations to prevent operator exposure to potentially harmful UV radiation.

MY-PCR prep stations are bench-top personal clean zones allowing the operator to amplify RNA and DNA while minimizing the potential for background from airborne contaminants.

Note: MY-PCR and FE-PCR are not biological safety cabinets. They are designed to provide process protection only from potential airborne contaminants found in the laboratory space.

MY-PCR WORKSTATIONS FEATURES

1. Class 100 vertical laminar flow air
2. 15-Minute UV light timer
3. Clear polycarbonate construction with chemically impervious polypropylene work surface
4. UV shelf with integrated pipette holder
5. Electrical cord pass-through port

Sash position switch and overlap to prevent UV light exposure

HEPA Filters, Pre-Filters, UV bulbs, additional ports and shelves also available.

PR1MA™ Sealing Mats

PR1MA™ 96-Well PCR Sealing Mats are made of silicone, dimensions are 4.67 in. (L) x 2.90 in. (W).

• Pierceable
• Designed for an evaporation-resistant seal to minimize sample loss
• For use in medical, pharmaceutical, food industries, and molecular biology
• Certified free of detectable RNase, DNase, DNA, and PCR inhibitors

PR1MA™ Taq

• Ideal for routine PCR applications as well as genotyping, colony PCR, and fast PCR
• Improved template affinity and solubility for higher enzyme activity and greater yields
• Proprietary buffer optimized for a variety of assay conditions
• Conveniently supplied as a 2X Master Mix or in a 2-tube format of polymerase and separate 5X buffer

PR1MA™ Taq provides superior results for routine applications. Modified to improve DNA-binding, this polymerase offers higher solubility and greater template affinity, resulting in consistently superior performance. PR1MA™ Taq Polymerase exhibits a 5' to 3' nuclease activity, but no 3' to 5' (proofreading) activity and works well with a wide range of DNA templates including GC-rich sequences.

The polymerase is supplied with a 5X buffer containing MgCl2 and a proprietary mix of enhancers (dNTP's not included). For convenience, PR1MA Taq is also available in a ready-to-use 2X Master Mix - just add primers and template DNA. Our "Red Dye" Master Mix incorporates a red-loading dye that allows amplified samples to be loaded directly on an agarose gel.  The red color aids in visualization during pipetting, and higher density of the buffer ensures that the samples will drop into the gel wells.

TAKE ADVANTAGE OF THIS SPECIAL PRICING

 on the MultiGene™ OptiMax Gradient Thermal Cycler TC9610 - now $3,088.50  $2,849.00!!

 

Extended Promo!! Now valid 04/01/24 - 06/30/24. Not to be combined with any offers or discounts. No institutional pricing. All terms apply.

 

MultiGene™ OptiMax Thermal Cycler

This simple-to-program PCR thermal cycler unit is compact in design and built to perform. Programming is intuitive with the large display and multiple pre-programmed templates supplied with the unit.

The OptiMax comes with standard built-in 2-step, temperature optimization, touchdown, and time increment protocols that are easy to adjust to meet your cycling needs. You can select lid temperature or turn it off depending on your needs. The faster ramp rates allow more work to be done in a workday.
 

Additional Thermal Cycler Product Features:

• Unique Flexible Programming with FlexTemp™ technology
• Fast ramp rates 5°C heating / 3.5°C cooling
• Simple user interface
• No condensation after overnight cooling at 4°C
• 3-Year Warranty

Distinct Heat Optimization

The MultiGene™ OptiMax employs a new protocol optimization process improving on older gradient features. This “Better than Gradient” capability allows users to select up to 6 distinct annealing temperatures to be run simultaneously. This is ideal for method optimization as well as applications like genotyping.

The OptiMax utilizes FlexTemp™ technology that effectively separates the 96-well plate into 6 – (4 x 4) temperature regions that allow you to pick the temperatures you want to test against. These regions are easily identified by the blue and black squares visible on the microplate platen. Instead of having the thermal cycler choose temperatures for you, you can choose any temperatures you want within a 24°C range.

The MultiGene™ OptiMax has a heating rate of 5°C /sec and a cooling rate of 3.5°C/sec. We have minimized overshooting and undershooting so that your reactions only see the temperatures they are supposed to see. This also leads to faster runs time.

Intuitive User Interface

The MultiGene™ OptiMax utilizes an intuitive user interface. This user interface is friendly to the laboratory environment and consistent with what our customers previously enjoyed in our earlier models. It can be used with lab gloves even if wet. We have improved the ramping speed and eliminated overshooting and undershooting which contributes to longer run times.

Fully Adjustable Heated Lid

The OptiMax’s heated lid is fully adjustable to provide optimum pressure for use with a broad range of tubes and microplates. For optimum performance, the lid can also be programmed to hold different temperatures between 60° to 65°C and 100° to 115°C. The OptiMax lid prevents condensation and slides away and back from the user to provide full access to samples and reduce the risk of coming in contact with a hot surface.

Specifications 

• 96-well, 8 x 12 format
• Holds 0.2ml PCR tubes, strip tubes, and plates (does not fit full skirted plates)
• Supplied with clear lift-off lids

• Replaces hazardous Ethidium Bromide (EtBr)
• Better sensitivity than EtBr
• Detect as little as 0.1ng of DNA
• Two types available
• PS Pre Stain
• LD Loading Dye
• Excitation by UV light or blue light
• Compatible with Accuris SmartBlue™ Transilluminator
• Ships at ambient temperature (stored at 4°C)

PR1MA™ SmartStain™ PS (Pre-Stain) can be used as a direct replacement for Ethidium Bromide in agarose and polyacrylamide gel electrophoresis. The stain emits green fluorescence when bound to dsDNA or ssDNA and emits red fluorescence when bound to RNA. PR1MA™ SmartStain™ PS exhibits excitation peaks at 290 nm and 490 nm, allowing it to be used with UV and blue light.

Protocol:

• Prepare 100 mL of agarose or polyacrylamide solution.
• Add 5 µL of PR1MA™ SmartStain™ to the gel solution before pouring gels.
• For enhanced results, add PR1MA™ SmartStain™ PS to the running buffer at a ratio of 5 µL per 100 mL. Adding PR1MA™ SmartStain™ PS to the running buffer will result in increased sensitivity and better detection of small quantities of nucleic acid.
• After electrophoresis is complete, view the gel using a UV or blue light illuminator   

IsoFreeze PCR and IsoFreeze PCR SBS are innovative, high performance, personal chiller racks. Using proven IsoFreeze temperature maintenance technology, the filled racks maintain a cool(refrigerator) environment on the bench or on the workstation platform. IsoFreeze gives a chilled and stable environment for PCR reagents, cells, enzymes or other temperature sensitive reagents.

Industry Standard format tubes, strips and 96 well plates, in both standard and low profile well shape, are accommodated and are efficiently cooled in PCR, QPCR and many other applications.

IsoFreeze PCR Racks are manufactured using polypropylene containing novel, temperature dependent color-change dye giving simple, at-a-glance, temperature indication for each well. There is a dramatic change to a contrasting color at 7C. IsoFreeze gel technology means the wells will stay cool for up to 4 hours (see charts below), depending on ambient temperature and other factors.

IsoFreeze PCR can keep your sample cool for over 4 hours!

IsoFreeze PCR SBS high performance is ideal for your automated systems!

IsoFreeze PCR and IsoFreeze PCR SBS available in 2 color change formats!

 
PR1MA™ Taq Plus

• Higher fidelity for long PCR amplicons, up to 35 kb
• Ideal for problematic templates
• Better sensitivity and higher activity for low-copy and long PCR
• Enzyme of choice for TA cloning

PR1MA™ Taq Plus, an optimized blend of our Hot Start Taq and a proofreading polymerase, provides 5X better fidelity than wild-type Taq and increased enzyme activity. It is the perfect choice for GC and AT rich templates, low copy number, and long PCR.

PR1MA™ Taq Start Taq DNA Polymerase 

PR1MA™ Taq Plus Master Mix, 2X Concentration

TAKE ADVANTAGE OF THIS SPECIAL PRICING! 

Azure Cielo 6 qPCR (AIQ060) PROMOTIONAL PRICE: $16,895
 

*Includes: Azure Cielo 6, analysis software, and 18 months of warranty.

 

Promo is extended! Now valid 04/01/24 - 06/30/24. Not to be combined with any offers or discounts. No institutional pricing. All terms apply.

Azure Cielo 3 & 6

The Azure Cielo Real-Time PCR system provide accuracy and sensitivity needed for research, with intuitive touch screen software.

Product Features

• Flexibility:  Engineered for a wide variety of qPCR applications, with high sensitivity and wide dynamic range.

• Intelligent workflow: The user interface is a simple touch screen assay setup.  Protocols can be saved to make assay set up easy and reproducible.

• Reliability: Cielo's optics and thermal block are designed to deliver the same uniformity and reproducibility over at least 1,000 qPCR experiments, giving you confidence in the reliability of the instrument.

• Connectivity: An external PC is not required to run the instrument.

• Data Transfer: This can be performed via Ethernet, Wi-Fi or USB.

Operation - A standalone qPCR system with 10.3” touchscreen interface
Optics - 3 or 6 dye channel filters with LED based excitation for multiplexing
Speed - Fiber optic detection system scans 16 wells simultaneously
Durability - Tested reliability for over 1000 continuous runs
Connectivity - Easily export data via Wi-Fi, ethernet, USB or LAN
Capacity - 96-well format for semi-skirted or un-skirted plates and tubes
Thermal Performance - Marlow Peltier based block with 12 temperature gradient zones, ramp rates up to 6.0°C/sec
Chemistry - Supports reaction volumes between 10 µl–150 µl, open source chemistry, including FAST ~20 minute run-times
Sensitivity - Detect 2-fold differences in target quantities in singleplex reactions and up to 1 copy of amplified DNA, 10 logs of dynamic range

Competitor Q uses a single light source to excite all samples.

Differences in light path length lead to differences in excitation, which requires normalization. Though Competitor Q has said that they do not require normalization dyes, this is not reflected in the data as the data CV values for replicates is significantly higher.

Why doesn’t the Cielo require ROX dye?

 The Cielo does not require ROX dye because it uses in-well detection technology. The light path for excitation and reading emission are collimated directly into the well. As such, the light path length is always the same for each well, so there is no need to normalize for varying light path length. The data from the Cielo is consistently uniform and reproducible.

 

 
PR1MA™ qMAX™ Probe qPCR Mix

• Same high efficiency for multiplex and singleplex reactions
• Includes PR1MA Hot Start Taq Polymerase for greater specificity and accuracy
• Compatible with popular hydrolysis and beacon probes
• Ready to use 2X mastermix
• Early Ct values and detection across a broad dynamic range

Optimized for use with TaqMan™, Scorpions^® and molecular beacon probes, qMax Probe qPCR Mix is a ready-to-use formulation for real-time quantitative assays. qMax Probe utilizes PR1MA™ Hot Start Taq Polymerase or robust PCR with a variety of templates. A specially formulated buffer provides optimal conditions for both superior polymerase function and probe detection, resulting in earlier Ct values and a broad detection range. Complicated, multiplex reactions can be performed without any loss in performance or decrease in detection. The 2X mix requires little, if any optimization and can be used with both fast and standard protocols.

PR1MA™ qMax Probe qPCR Mix can be used to detect any DNA template, including genomic DNA and cDNA. Available in high, low and no ROX formulations, qMax Probe is compatible with most qPCR instruments.

*Please note these products ship on dry ice.  Appropriate shipping charges apply unless otherwise noted on a quote.

• Designed for convenience & versatility, this rack can be used while you are working or as a storage rack.
• It includes a lid and can accomodate 32 x 0.2 ml PCR tubes, 24 x 1.5/2.0 ml & 16 x 0.5 ml microtubes.
• Tube wells are alpha-numerically matrixed with raised bold type for quick identification.
  
• If you use cycle plates, 8 or 12 tube strips, or lage quantities of 0.2 ml PCR tubes, the WRI-4A is for you.
• Four retainer clips hold the tray firmly in place, yet allow fast and convenient removal when necessary.
• Available with a clear lid and five flourescent colors: blue, green, orange, pink, yellow, or assorted.

MyGo Mini S

• Small format 16 x 0.1 mL QPCR System with Taqman Multiplexing at an amazing price.
• Amazing accuracy and easy to use software that allows you to control 100s of systems with one PC and analyze the data at the same time.  Windows, Linux and Mac OSX
• Solid state reliability with up to 5-year warranty

Precision and Performance
The MyGo Mini demonstrates superior intra-run and inter-run analytical precision in DNA quantification and melting point analysis. Users can now address biological phenomena with subtle effects on gene expression, or pathogen levels, for example discriminating 10% differences in transcript concentrations. For users performing HRM-based analysis of genetic variants, the combination of thermal control, optical data quality and HRM data analysis of the MyGo Mini S system provide compelling functionality. The system can discriminate all classes of SNP, including Class 4 SNPs via HRM.

Users can now address biological phenomena with subtle effects on gene expression, or pathogen levels, for example discriminating 10% differences in transcript concentrations.

For users performing HRM-based. analysis of genetic variants, the combination of thermal control, optical data quality and HRM data analysis of the MyGo Mini system provide compelling functionality. The system can discriminate all classes of SNP, including Class 4 SNPs via HRM.
 

Easy Analysis
The MyGo Mini system comes with easy-to-use software, and robust, high performance algorithms which provide accurate results from complex data. Automated analysis of complex HRM data makes this powerful technique more accessible to non-experts, and minimizes operator-dependent variability in data analysis. Automated report generation makes life easier for service providers, and users operating within GLP quality systems.

Automated report generation makes life easier for service providers, and users operating within GLP quality systems.