PR1MA™ Mini Gel Electrophoresis System

• Complete system, includes gel box, power supply and casting sets
• Quick and easy gel casting, no tape, clamps or spacers required
• Compact design, footprint: 7 x 5.1 in.

The PR1MA™ Mini Gel electrophoresis system includes all of the equipment that you need to get up and running: gel tank, power supply, and two casting sets. Ideal for personal use, small laboratories, or the classroom, this gel box/power supply combination is compact and easy to use.

The mini gel box attaches directly to the power supply for a secure connection every time. Selectable parameters include 3 preset voltages – 35V, 50V or 100V – and a 99-minute timer. All are set using the membrane keypad.

The safety lid is vented to help dissipate heat generated during electrophoresis and features a viewing area above the gel. Current will only run through the system when the lid is properly in place on top of the gel box.

Trays and casting stands for both 10.5 x 6 cm and 5 x 6 cm gels are included with the PR1MA™ Mini system, as are double-sided combs. The smaller tooth combs are compatible with multi-channel pipettes. Casting is quick and easy – just place the tray in the casting stand and pour. The trays feature a dark contrast strip to help visualize wells while loading and lines that fluoresce under UV and blue light to track the progress of the run.

Specifications

• Convenient cDNA synthesis and PCR in a single tube from 1 pg total RNA
• Formulated for highly specific and sensitive RT-PCR from any RNA templates
• Incorporates thermostable reverse transcriptase and Hot-Start Taq for preparation at room temperature

The PR1MA™ One-Step RT-PCR Kit has been formulated for cDNA synthesis and subsequent PCR in a single tube for end-point analysis. This latest generation RT-PCR Kit consists of an MMLV-derived, thermostable Reverse Transcriptase (45°C to 55°C), an advanced RNase Inhibitor and PR1MA™ Hot Start Taq for ultra-sensitive one-step RT-PCR from as little as 1pg total RNA starting material.

The optimized buffer chemistry allows for efficient reverse transcription and PCR of problematic sequences with significant secondary structure (such as GC-rich targets). The PR1MA One-Step RT-PCR Kit is ideal for determining the presence or absence of RNA templates and quantifying expression through qualitative analysis of RNA transcription levels. The kit also efficiently synthesizes double-stranded cDNA for subsequent gene expression analysis.

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 Item PR2110-100 has been discontinued by the manufacturer;
however, we will be continuing to supply the other quantities.

 

In place of item PR2110-100, please order items PR2110-50 or PR2110-200

PR1MA™ qMAX™ cDNA Synthesis Kits

PR1MA™ now offers two cDNA Synthesis Kits, to meet a range of requirements.
 
The original cDNA Synthesis Kit is a 2-tube format for easy reaction setup and is ideal for 4 pg to 0.5 µg of input RNA. One tube includes our exceptionally stable Reverse Transcriptase combined with a potent RNAse inhibitor, and the other tube contains a 5X reaction buffer with an optimal mixture of anchored oligo (dT) primers and random hexamers to produce a non-biased population of cDNA. 
 
The 1st Strand cDNA Synthesis Flex Kit includes four components: a high-capacity Reverse Transcriptase, an optimized 5X buffer, separate solutions of oligo (dT) primers, and random hexamer primers. This multi-component format is ideal for 10 pg to 2.0 µg of input RNA and allows for greater flexibility in assay design.

 
PR1MA™ qMax cDNA Synthesis Kits
  

Item	Description	Volume (20 µL)
PR2100-C-S	PR1MA™ qMax™ cDNA Synthesis Kit	10 Reactions (Sample)
PR2100-C-25	PR1MA™ qMax™ cDNA Synthesis Kit	25 Reactions
PR2100-C-100	PR1MA™ qMax™ cDNA Synthesis Kit	100 Reactions
PR2100-C-250	PR1MA™ qMax™ cDNA Synthesis Kit	250 Reactions

 

PR1MA™ qMax First Strand cDNA Synthesis Flex Kits  
 

Item	Description	Volume (20 µL)
PR2110-S	PR1MA™ qMax™ First Strand cDNA Synthesis Flex Kit	10 Reactions (Sample)
PR2110-50	PR1MA™ qMax™ First Strand cDNA Synthesis Flex Kit	50 Reactions
PR2110-100	PR1MA™ qMax™ First Strand cDNA Synthesis Flex Kit	100 Reactions
PR2110-200	PR1MA™ qMax™ First Strand cDNA Synthesis Flex Kit	200 Reactions

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PR1MA™ dNTPs

• Supplied as a ready-to-use 40 mM mix or a set of 4 separate 100 mM solutions
• Free of impurities and inhibitors that reduce sensitivity and yield
• No nuclease, protease or nickase activity
• >99% pure, purified by HPLC
• 24-month shelf life

PR1MA™ dNTP's are purified by HPLC in a strict process that results in greater than 99% purity. The stringent purification process eliminates PCR inhibitors such as tetraphosphates and pyrophosphates that can interfere with the sensitivity of your PCR and reduce yields.
The 40 mM dNTP Mix is a single tube that contains premixed dNTPs at a concentration of 10 mM each. The 100 mM dNTP Set contains four individual tubes, one each of dATP, dCTP, dGTP and dTTP. The nucleotides are supplied in ultra-pure water as an ammonium salt. Both the set and mix are stable for 24 months when stored at -20ºC.

Extensive quality control testing is performed to ensure the dNTPs are free of nuclease, protease and nickase activity. Each lot is performance tested in standard PCR, long PCR and qPCR reactions to assess reproducibility and sensitivity.

PR1MA™ dNTP Mix

PR1MA™ dNTP Set  

Item	Description	Volume
PR3101-S-1	PR1MA™ 100 mM dNTP Set, dATP, dCTP, dGTP, dTTP	250 uL / 25 umol each
PR3101-S-4	PR1MA™ 100 mM dNTP Set, dATP, dCTP, dGTP, dTTP	1 mL / 100 umol each
PR3101-S-20	PR1MA™ 100 mM dNTP Set, dATP, dCTP, dGTP, dTTP	20 x 250 uL / 25 umol each

 

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Mystaire® CleanPrep™ dead air boxes are circulation-free enclosures that establish an ideal work area to protect against contamination in sensitive PCR amplification reactions. CleanPrep™ workstations are constructed from thermally-fused polypropylene that offers exceptional chemical resistance, does not adsorb moisture and can be easily cleaned and decontaminated between amplifications using a variety of disinfectant solutions.Seamless polypropylene design for easy decontamination between amplifications.

FEATURES:

1. Clear polycarbonate access with overlap and safety position switch
2. UV shelf with integrated pipette holder - accommodates four pipettors
3. High intensity fluorescent lamps and shortwave UV light
4. Available in 36 and 48 inch widths with nominal 24 inch depth
5. Circulation-free enclosure for PCR applications

HEPA Filters, Pre-Filters, UV bulbs, additional ports and shelves also available.

TC-96 Turbo™ 2D Gradient Thermal Cycler

• Exceptionally fast cycling – speeds up to 9°C/second
• 2D Gradient for simultaneous optimization of annealing and denaturation temperatures
• Large, folding 10” touch screen
• Program wizard for quick and easy protocol entry
• Optimize results with MIDSCI™ PCR reagents

The Benchmark™ TC-96 Turbo™ 2D Gradient Thermal Cycler is designed for fast cycling and quick protocol optimization. Exceptionally easy to program, the unit features tight temperature control and excellent block uniformity for consistent, reliable results.

Many thermal cyclers offer users the option to select a temperature gradient across the block to test a range of annealing temperatures simultaneously. The TC-96 Turbo™ offers a 2D gradient – across both the columns and rows – allowing for optimization of annealing and denaturation temperatures in the same run. The ability to optimize both steps provides for an increase in yield and specificity of complex or GC rich templates. Temperature ramp rates as fast as 9°C per second allow reactions to be performed quickly – up to 20% faster than other popular thermal cyclers. Temperature change is precisely controlled by a proprietary algorithm that simulates sample temperature and allows samples to arrive at the programmed temperature without any lag or overshoot.

All operating parameters are controlled and monitored from the large, full color, 10” touch screen. The screen can be angled toward the user for easy access and folded back against the instrument when not in use to save space. Entering and editing programs is intuitive with the program wizard. Programming features include adjustable ramp rates, temperature and time increments/decrements, password protected multi-user login and a memory for 30,000 programs.

TC-96 Turbo™ will accommodate 96 x 0.2 or 96 x 0.1 mL tubes as well as non-skirted 96 well plates. The height of the heated lid is auto-adjusting to prevent condensation for all of the accepted consumables. Temperature of the lid is adjustable.

Compatible with select MIDSCI™ PCR reagents, click here to see options.

*For research use only.

Specifications

 
MultiCycler™ Multi-block Thermal Cycler

Designed for laboratories wanting flexibility in their applications, Benchmark’s MultiCycler™ Thermal Cyclers offer multiple, independent cycling blocks in a single unit.

• Dual or triple block models are available, both offering extremely fast cycling profiles
• Large touchscreen interfaces
• Self-adjusting heated lids
• Gradient capabilities
• Easy-to-use program wizard.

All programming features are accessed from the large, 10” full-color touch screen. The screen can be angled towards the user when in use and then tucked away to save space. A program wizard guides users through the simple protocol setup process. Advanced programming features include gradient, adjustable ramp rates, and time increments and decrements. For multi-user labs, password protection prevents interference with an existing run. A USB provides for unlimited program storage and easy transfer of protocols between MultiCycler™ machines.

The auto-adjusting heated lid prevents condensation and evaporation of samples. Lid temperature can be programmed, and when the block temperature is below 35°C, the lid automatically shuts off. Peltier units allow for exceptionally fast heating and cooling of the blocks. Temperature change is precisely and accurately controlled within tight tolerances by a proprietary algorithm that simulates sample temperature, resulting in fast runs and reproducible results.
 
The wells are arranged in a 4×8 and 6×8 format, allowing for the use of PCR strip tubes in both models. Each block is independently controlled.

Specifications

myGel InstaView™ System | Complete System with Power Supply

• View DNA migration and separation as it happens
• Blue light illumination - won't damage DNA
• Capture high-resolution images without removing the gel from the tank

The myGel InstaView™ comes complete with everything needed to cast, run, and view mini electrophoresis gels, including an integrated power supply. One large (10.5 x 10 cm) or small (10.5 x 6 cm) gel is accommodated by the gel tank. Gels are easily poured using the supplied trays, combs, and leak-proof casting stand. The power supply connects directly to the gel tank – no more leads to contend with. The connection is guaranteed to be right, every time.

Output voltage can be adjusted to 35V, 50V, or 100V and run time can be set for up to 99 minutes. A magnetic interlock between the gel tank and the lid prevents current from running through the system when the lid is not properly in place.

Once the myGel InstaView™ System is set up, simply slide the mini LED transilluminator into place under the tank and at any time turn it on to view the run in real-time. An array of blue LEDs emits bright blue light at a wavelength of 465 nm for optimal excitation of common green fluorescent stains such as SmartGlow™, SYBYR™ Green, and Gel Green, without damaging DNA. An orange filter is incorporated into the lid for visualizing DNA bands without any additional equipment or special glasses. When it’s time to document results, place the included gel imaging enclosure over top of the myGel InstaView, and use the camera app on any smartphone to capture high-resolution images without removing the gel from the tank.

Images are easily shared via email or text message or printed.
 

Specifications

InstaView™ Gel Tank

For labs that prefer to use a separate power supply, the InstaView™ Gel Tank is the perfect choice for running, viewing, and documenting electrophoretic separations. The InstaView™ Gel Tank accommodates one large (10.5 x 10 cm) or one small (10.5 x 6 cm) gel tray and is supplied with trays, combs, and a leak-proof casting stand. The gel tank lid includes attached power leads which prevent the unit from operating without the lid properly in place.

Any standard power supply can be connected, including the Accuris myVolt™ Mini and myVolt Touch. The InstaView™ Gel Tank is shaped to allow the mini-LED transilluminator (included) to slide underneath. Blue light, at a wavelength of 465 nm, provides excitation of common green fluorescent stains such as SmartGlow™, SYBR™ Green, and GelGreen. An orange filter is incorporated into the gel tank lid for visualizing the DNA in the gel box without any additional equipment or glasses. The transilluminator can be turned on at any time during a run to track progress and automatically shuts off after 5 minutes.

After electrophoresis, simply place the included imaging enclosure over the InstaView™ Gel Tank to capture high-resolution images with a smartphone. Images are easily printed or shared via email or text message.

Specifications
 

Safety	Physical interlock between lid and tank
Buffer Volume	230 mL
Gel Capacity	1-10.5 x 10 cm or 1-10.5 x 6 cm gels, 0.5 cm thick
Transilluminator	Blue LED, 465 nm
WxDxH (w/enclosure)	7.5 x 8.25 x 5 in /19 x 21 x 14 cm
Weight	1.65lb/0.75kg
Electrical	115V, 60Hz or 230V, 50Hz
Warranty	2-year

  

System & Accessories
 

PR1MA™ qMAX One-Step RT-qPCR Kits

• RNA to cDNA to qPCR in one tube
• High purity enzyme formulation for enhanced stability and performance
• PR1MA™ Hot-Start Taq allows for preparation at room-temperature
• Compatible with all qPCR instruments
• Available for green fluorescence or probe detection

Both One-Step Kits are compatible with standard and fast cycling protocols and provide increased sensitivity, speed and reproducibility for a broad range of samples and targets.  The polymerase mix is available with different levels of ROX reference dye for compatibility with all qPCR instruments.

Two versions of our One-Step qPCR Kits are available:

PR1MA™ qMAX Green One-Step Kits incorporate our proprietary intercalating dye which exhibits higher fluorescent and lower PCR inhibition than other popular dyes such as SYBR.

PR1MA™ qMAX Probe One-Step Kits are optimized for use with popular TaqMan, Scorpions, and molecular beacon probes.

*Please note these products ship on dry ice.  Appropriate shipping charges apply unless otherwise noted on a quote.

Proudly made in the USA! Click here for more made in America products!
  

IBI RT-PCR Certified and Nuclease-Free Water is ideal for all applications in a molecular biology lab including PCR, RT-PCR, restriction enzyme
assays, modifying enzyme assays, transfection, cloning, transformation, and all general molecular biology lab procedures.

IBI PCR Grade water is manufactured under stringent conditions. The purification process includes continuous deionization, reverse osmosis,
UV-treatment, 0.2µm filtration, followed by steam sterilization in an autoclave.

• RNA to cDNA to qPCR, in one tube
• High purity enzyme formulation for enhanced stability and performance
• Accuris Hot-Start Taq allows for preparation at room-temperature
• Blue dye facilitates pipetting and visualization in plates
• Available for green fluorescence or probe detection
• Multiplex formulation available for multiple target amplification