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The RNase-Free DNase I set ensures complete DNA removal from RNA extracted using IBI's RNA extraction kits for use in DNA sensitive downstream applications. This is an optional treatment as IBI's spin column technology yields RNA with the majority of DNA removed. Without using DNase treatment the extracted RNA can be used in downstream applications which are not DNA sensitive. This set can be used for both in column DNase digestion and DNase digestion in solution.

Item#:
ASDNARNAKIT2

Advantages

  • Sample: Tissue, rodent tails, ear punches, fresh or frozen blood, serum, plasma, buffy coat, body fluids, cultured cells, amniotic fluid, FFPE, hair, insects
  • Yield: Up to 5 µg of gDNA from fresh whole blood samples
  • Format: genomic DNA spin column
  • Operation Time: Within 20 minutes
  • Elution Volume: 30-100 µL
  • Kit Storage: Dry at room temperature (15-25°C) for up to 1 year

Introduction
The gMax Mini Kit is optimized for genomic, mitochondrial and virus DNA purification from whole blood (fresh blood and frozen blood), tissue, formalin-fixed paraffin-embedded tissue (FFPE), amniotic fluid and insects in one convenient kit. This DNA extraction kit uses Proteinase K and chaotropic salt to lyse cells and degrade protein, allowing DNA to bind to the glass fiber matrix of the spin column. Contaminants are removed using a Wash Buffer and the purified genomic DNA is eluted by a low salt Elution Buffer, TE or water. The entire procedure can be completed within 20 minutes without phenol/chloroform extraction or alcohol precipitation. The purified DNA (approximately 20-30 kb) is suitable for use in PCR or other enzymatic reactions.

Quality Control
The quality of the gMax Mini Kit is tested on a lot-to-lot basis by isolating genomic DNA from 200 μL of whole human blood. The purified DNA (5 μg with an A260/A280 ratio of 1.8-2.0) is quantified with a spectrophotometer and analyzed by electrophoresis.

Kit Components


Component

IB47280

IB47281

IB47282

GST Buffer

3 mL

30 mL

75 mL

GSB Buffer

4 mL

40 mL

75 mL

W1 Buffer

2 mL

45 mL

130 mL

Wash Buffer*
(Add Ethanol)

1 mL
(4 mL)

25 mL
(100 mL)

50 mL
(200 mL)

Proteinase K**
(Add ddH2O)

1 mg
(0.10 mL)

11 mg x 2
(1.10 mL)

65 mg
(6.50 mL)

Elution Buffer

1 mL

30 mL

75 mL

GD Columns

4

100

300

2 mL Collection Tubes

8

200

600

*Add absolute ethanol (see the bottle label for volume) to Wash Buffer then mix by shaking for a few seconds. Check the box on the bottle. Be sure and close the bottle tightly after each use to avoid ethanol evaporation.

**Add ddH2O to Proteinase K (see the bottle label for volume) then vortex to ensure Proteinase K is completely dissolved. Check the box on the bottle. Once it is dissolved completely, centrifuge for a few seconds to spin the mixture down. For extended periods, the ddH2O and Proteinase K mixture should be stored at 4°C.

Item#:
ASDNARNAKIT4

The 96-Well Genomic Plant DNA Kit provides an efficient method for isolating total DNA (genomic, mitochondrial, and chloroplast DNA) from plant tissue and cells. Samples are initially disrupted by grinding in liquid nitrogen, followed by lysate treatment with RNase A. The unique GR Buffer is able to lyse most common plant samples and also samples high in polysaccharides. DNA phenol extraction is not required and the entire procedure can be completed in 1.5 hours. The isolated total DNA is ready for use in PCR, Real-time PCR, Southern Blotting, mapping, and RFLP.


Sample Size:

Fresh or dry plant tissue

Format:

96-Well Plates

Expectant Yield:

Up to 80ug/well

Operation Time:

90 min

 

Ordering Information
IB47270 IBI Genomic Plant DNA Sample Kit, 96-Well, 2 x 96 preps
IB47271 IBI Genomic Plant DNA, 96-Well, 4 x 96 preps
IB47272 IBI Genomic Plant DNA, 96-Well, 10 x 96 preps
Item#:
ASDNARNAKIT20

The 96-Well Genomic DNA Kits are designed for high-throughput purification of total DNA (including genomic, mitochondrial and viral DNA) from whole blood and a variety of animal tissues or cells. This method uses Proteinase K and a chaotropic salt to lyse cells and degrade proteins. DNA in the chaotropic salt is bound by the glass fiber matrix of each well. Once any contaminants have been removed, the purified DNA is eluted by a low salt elution buffer or water. The entire procedure can be completed in 1 hour without phenol extraction or alcohol precipitation. These kits can be used for manual filtration or with robotic handling systems, and purified DNA with approximately 20-30kb is suitable for PCR or other enzymatic reactions.

Sample Size:

Up to 25mg of animal tissues, mouse tails or swabs
cultured animal cells  (up to 1 x 10), bacterial cells (up to 1 x 10) and fungus cells (up to 5 x 10)

Format:

96-Well Plates

Operation:

Centrifuge/Vacuum manifold

Binding Capacity:

Up to 30ug per well

Operation Time:

60 minutes

Item#:
ASDNARNAKIT19
  • Optimized for use with all of IBI 96-well kits
  • The thin upper portion of the manifold is designed to reduce cross contamination
  • Allows for the most effective extraction and purification of plasmid DNA, genomic DNA, viral DNA & RNA, total RNA and PCR products
  • Materials: Manifold - made of anodized aluminum, Gasket - Ethylene propylene, O-Ring - Silicone
  • Dimensions: 17x12x8cm
  • Maximum vacuum: Approx. 71cm Hg (28 in Hg) (-13.7psi)
Item#:
IB47500
Your Price:
1546.05
Each

    IBI DNA/RNA/Protein Extraction Kits

  • Sample Size: up to 5×106 cultured animal cells/up to 25mg animal tissue /up to 500µl whole blood/up to 200µl biological fluids (serum, plasma)
  • Expectant Yield: up to 9µg of genomic DNA/20µg of total RNA/120µg of protein from 1×106 of HeLa cells
  • Operation Time: DNA/RNA purification within 25 minutes, protein precipitation within 50 minutes
  • Elution Volume: 50  200µl (genomic DNA)/25  50µl (total RNA)
  • Format: genomic DNA spin column and total RNA spin column

The IBI All Prep DNA RNA Protein Mini Kit provides an efficient method for purifying genomic DNA, total RNA, and total protein simultaneously from cultured cells, animal tissues, whole blood, and biological fluids. Chaotropic salt is used to lyse cells and inactivate DNases and RNases, allowing DNA to bind to the genomic DNA spin column. The flow-through can then be transferred to the RNA Spin column for RNA binding. Contaminants are effectively removed using wash buffers followed by pure genomic DNA elution in a low salt buffer and pure total RNA elution in RNase-free water.

DNA/RNA purification can be completed in 25 minutes without phenol/chloroform extraction or alcohol precipitation, and protein purification can be completed in 50 minutes. The purified DNA, with approximately 20  30Kb, is suitable for use in PCR or other enzymatic reactions and the purified RNA (including miRNA) is ready for use in RT-PCR, Real-Time PCR, northern blotting, primer extension, mRNA selection, and cDNA synthesis. The purified proteins can be directly analyzed on a SDS-PAGE and subsequent western blot.

The quality of the All Prep DNA RNA Protein Mini Kit is tested on a lot-to-lot basis by isolating genomic DNA and total RNA from 1x106 cultured animal cells. The purified DNA and total RNA is quantified with a spectrophotometer and analyzed by electrophoresis on a 1% agarose gel.  The purified protein is quantified by Bradford assay analyzed on SDS-PAGE.

Specifications

Sample: Cultured cells, animal tissue, whole blood & biological fluids
Format: Genomic DNA spin column and Total RNA spin column.
Yield: up to 9ug of Genomic DNA / 20ug of Total RNA / 120ug of Protein from 1 x 106 HeLa cells.
Operation Time: Within 25 minutes
Elution volume: 50  200ul for Genomic DNA / 25  50ul for Total RNA.
Simultaneous extraction of Genomic DNA and Total RNA from cultured cells, animal tissues, whole blood and biological fluids.

Item#:
ASDNARNAKIT40

IBIs MIDI and MAXI plasmid kits use pre-packed ion-exchange resin columns to purify plasmid or cosmid DNA from bacterial cultures. In the process, the modified alkaline lysis method and RNase treatment are used for creating cleared cell lysate with minimal genomic DNA and RNA contaminants. Using a gravity-flow procedure, the plasmid DNA in crude lystate has been bound to the column. he contaminants can be washed off with a wash buffer. Finally, the purified plasmid DNA is eluted by a high salt buffer and then precipitated with isopropanol for desalting. The entire procedure can be completed in less than 2 hours and the obtained high purity plasmid DNA is suitable for transfection, sequencing reactions, PCR, and in-vitro transcription.

Sample size: MIDI50 mL for high copy plasmid, 100 mL for low copy plasmid
MAXI- 100 mL for high copy plasmid, 250 mL for low copy plasmid
Format Ion-Exchange Resin Column
Operation Gravity Flow
Binding Capacity MIDI:  500 ug/ MAXI-1mg
Expectant Yield MIDI-up to 200 ug of plasmid DNA/MAAX-up to 1 mg of plasmid DNA
Purity Equal to that obtained by 2X CsCL-Gradient Centrifugation
Operation Time 120 minutes or less
Applications Transfection,: Sequencing, In vitro Trascrioption
Item#:
ASDNARNAKIT11

The Fast Ion Plasmid Maxi Kit (Endotoxin Free) uses pre-packed anion exchange resin columns to purify plasmid DNA from 100-250 ml of bacterial cultures. Modified alkaline lysis method (1) and RNase treatment are used for obtaining clear cell lysate with minimal genomic DNA and RNA contaminants. Once the plasmid DNA has been bound to the column, the contaminants can be washed off using the wash buffer. Finally, the purified plasmid DNA is eluted by a high salt buffer and precipitated with isopropanol for desalting. The entire procedure can be completed in 120 minutes and the obtained high purity plasmid DNA is suitable for transfection, sequencing reactions, PCR and in-vitro transcription.

Specifications:
Endo Free Midi
Endo Free Maxi
Sample:
50ml culture for high-copy
100ml culture for high-copy
100ml culture for low-copy
250ml culture for low-copy
Yield:
up to 200ug of plasmid DNA
500ug to 1mg of plasmid DNA
Format:
gravity flow
gravity flow
Time:
approx 120 min.
approx 120 min.
Item#:
ASDNARNAKIT12
  • Sample: Gram (+) positive and Gram (-) negative bacterial cells
  • Yield: Up to 30 µg of gDNA - (1 x 10 Escherichia coli: 25-30 µg, 1 x 10 Bacillus subtilis: 10-15 µg)
  • Convenient: Includes Gram+ Buffer for preparing lysozyme solutions and to speed up sample preparation
  • Format: Genomic DNA spin columns
  • Operation Time: within 30 minutes
  • Elution Volume: 50-200 µL
  • Kit Storage: Dry at room temperature (15-25°C) for up to 1 year

The gBAC Mini DNA Bacteria Kit is optimized for genomic and viral DNA purification from Gram (-) negative and Gram (+) positive bacterial cells. Gram+ Buffer, when combined with lysozyme, will efficiently lyse bacterial cell walls consisting of the peptidoglycan layer. Chaotropic salt is used to further lyse cells and degrade protein, allowing DNA to easily bind to the glass fiber matrix of the spin column. Contaminants are removed using a Wash Buffer (containing ethanol) and the purified genomic DNA is eluted by a low salt Elution Buffer, TE or water. Phenol/chloroform extraction or alcohol precipitation is not required and the purified genomic DNA is ready for use in a variety of downstream applications.

Quality Control
The quality of the gBAC Mini DNA Bacteria Kit is tested on a lot-to-lot basis by isolating DNA from Escherichia coli (1x10) culture (OD600=1.3, 1 mL) harvested by centrifugation at 16,000 x g for 1 minute. 10 µL from a 50 µL eluate of purified DNA is analyzed by electrophoresis on a 1% agarose gel.

Items

IB47290

IB47291

IB47292

Gram+ Buffer*

1.5 mL

30 mL

75 mL

GT Buffer

1.5 mL

30 mL

75 mL

GB Buffer

2 mL

40 mL

100 mL

W1 Buffer

2 mL

45 mL

130 mL

Wash Buffer**
(Add Ethanol)

1 mL
(4 mL)

25 mL
(100 mL

50 mL
(200 mL)

Elution Buffer

1 mL

30 mL

75 mL

GD Columns

4

100

300

2 mL Collection Tubes

8

200

600

*Add lysozyme to Gram+ Buffer immediately prior to use. Once lysozyme is mixed with Gram+ Buffer, the solution can be stored for 2 weeks at 4°C.


**Add absolute ethanol (see the bottle label for volume) to Wash Buffer then mix by shaking for a few seconds. Check the box on the bottle. Be sure and close the bottle tightly after each use to avoid ethanol evaporation.

Item#:
ASDNARNAKIT3

The Large DNA Fragments Extraction Kit was designed to recover or concentrate DNA fragments (> 8 Kb) from agarose gel in 4 easy steps. Salts and enzymes can be effectively removed from the reaction mixture without phenol extraction. Typically, recoveries are up to 85% for Gel Extraction. The entire procedure can be completed in 45 minutes and the DNA is ready for use in PCR, Fluorescent or Radioactive Sequencing, Restriction Enzyme Digestion, DNA Labeling and Ligation.

Sample agarose gel
Recovery up to 85%
Format spin column
Operation time 45 minutes
Item#:
ASDNARNAKIT9

Efficient Gel Extraction and PCR Clean-up

The Gel/PCR DNA Fragments Extraction Kits are designed to recover or concentrate DNA fragments (50bp-10kb) from agarose gel, PCR reaction, or any other enzymatic reaction. This method uses a chaotropic salt and guanidine thiocyanate to dissolve the agarose gel and denature the enzymes. The DNA fragments in the chaotropic salt are bound to the glass-fiber matrix of the spin column. After washing off the contaminants, the purified DNA fragments are eluted by low salt elution buffer or water. Salts, enzymes, and unincorporated nucleotides can be effectively removed from the reaction mixture without phenol extraction or alcohol precipitation.

Sample:
up to 300mg agarose gel slice
up to 100µl PCR product or other enzymatic reaction
Format:
Spin columns
Operation:
Centrifuge/Vacuum Manifold
Binding Capacity:
10µg DNA
Expectant Yield:
80-90% for gel extraction
90-95% for PCR clean-up
Operational Time: 15min. for PCR clean-up/20min. for gel extraction
Applications:
PCR, Fluorescent or Radioactive Sequencing, Restriction Digests, DNA Labeling, Ligations
Item#:
ASDNARNAKIT6

The Genomic DNA Kit for Blood or Cultured Cells provides an efficient method for purifying total DNA (including genomic, mitochondrial and viral DNA) from whole blood, frozen blood, buffy coat, cultured animal/bacterial cells and fungus. Chaotropic salt is used to lyse cells and degrade protein, allowing DNA to bind to the glass fiber matrix of the spin column. Contaminants are removed using a Wash Buffer (containing ethanol) and the purified genomic DNA is eluted by a low salt Elution Buffer or TE. The entire procedure can be completed in 1 hour without phenol/chloroform extraction or alcohol precipitation, with an average DNA yield of 6 µ g from 200 µ l of whole human blood and up to 50µ g of DNA from 200µ l of buffy coat. Purified DNA, with approximately 20-30 Kb, is suitable for use in PCR or other enzymatic reactions.

 

MINI

MAXI

Sample Size:

up to 300µ l fresh whole blood, up to 10 cultured cells

10ml frozen blood, up to 10 cultured cells

Format:

Spin Column

Spin Column

Expectant Yield:

up to 50µ g DNA in 50-200µ l

up to 140µ g DNA in 1-2ml

Operation Time:

40 min or less

60 min or less


Item#:
ASDNARNAKIT16
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