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TPP Cryogenic Tubes


Perfect for long-term storage of Cell lines or Harvested Tissues in the freezer or cryogenic dewars (LN2 vapor phase)

  • Pronounced external thread for gas and aerosol tight seal
    • Enables a fitted seal to prevent leaking during storage, transportation or centrifugation
    • Protects sample integrity by reducing contamination
  • The external threading results in a smooth internal surface that eliminates undue loss of specimen material during removal.
  • Uniquely designed vial geometry voids the use of silicone gaskets
  • Self-standing tube with star bottom permits one handed opening/closing
  • Manufactured with the highest quality of virgin raw materials
    • Minimizes sample interaction with the centrifuge tube material and prevents leaching that decreases sample integrity
    • No additives, slip agents, biocides or plasticizers are utilized in the manufacturing process
  • White writing surface for easy labeling
    • On side of tube permits detailed description labeling
    • Ideal pairing with labeling TPP cap inserts
  • Highly visible white & accurate graduations
  • Supplied in convenient, puncture and tear-resistant re-closable bags with zipper and laser perforation
  • Permits ergonomic open/close under sterile conditions
  • Application-based options available
    • Cell line freezing & long-term storage
    • Sample storage and secure transport
    • Long-term storage of harvested tissues

 

Quality Assurance:

Sterile (gamma irradiated)

DNAse/RNAse, pyrogen, and heavy metal-free

 

Recommendations:

Always use appropriate safety equipment and practices when handling frozen polar tubes

TPP Polar Tubes are to be used for storage  in the gas/vapor phase

Avoid filling up the Polar Tubes to the maximal filling volume to avoid spillage of liquid sample due to expansion of liquids upon freezing

 

 

Material

Temperature Range

Max. RCF

Polypropylene (PP) Tubes

-196°C to 121°C

9,500 x g


 

 

PR1MA™ Autoradiography and Chemiluminescence Film for Western Blots

 

One autoradiography film for ALL your needs!


Many companies sell film solely on price without considering your needs. Some offer many different films making it difficult to decide what is best for your needs.

MIDSCI™ narrowed the Autoradiography Film field to PR1MA™ Blue Sensitive Film. It will work outstandingly well for almost 100% of all molecular labs' needs.
 

  • PR1MA™ Autoradiography Film is double emulsion
  • High signal sensitivity
  • For autoradiography of 32P, 33P, 35S, 125I, and 14C
  • Excellent lot-to-lot consistency
  • Exceptional clarity with extremely low backgrounds
  • Consistently superior results for exposure of blotting experiments and sequencing gels
  • Publishable grade autoradiographs
  • Optimized for chemiluminescence
  • Film works with calcium tungstate or blue rare earth screens
  • For use manually or with automatic processing
  • 100 sheets per box
  • Available in 3 sizes: 5 x 7 in., 8 x 10 in., and 14 x 17 in.

 

Specifications

 

PR1MA™ Blue Film BX
Item #SizeqtyFeatures
BX575 x 7 in.100/pkDouble Emulsion
BX8108 x 10 in.100/pk
BX141714 x 17 in.100/pk, 5pks/cs

 


Item#:
ASFILM

PR1MA™-Shell Plates - 384-Well, Full Skirted


Discover the next generation of PCR plates: Rugged Polycarbonate Frame + Polypropylene Wells

 

Engineered for unparalleled durability, PR1MA™-Shell PCR Plates are designed to accommodate all laboratory workflows and are ideal for use on automated liquid handling platforms.

 

PR1MA™-Shell sets a new standard in performance and convenience.

 

·       The polycarbonate frame is rigid and flat to facilitate a firm grip by robotic arms and ensure reliable placement

·       Polypropylene wells provide optimal thermal transfer and higher reaction efficiency

·       White well options enhance the fluorescence signal during qPCR

·       Wells are slightly raised to ensure evaporation-proof sealing with film, foil, or strip caps

·       ANSI/SBS footprint and well spacing

·       Vibrant colors offer a visual distinction between different samples or experiments

·       Certified free of detectable RNase, DNase, DNA, PCR inhibitors, and tested pyrogen-free

 

 

Trust MIDSCI™ for PCR consumables that deliver accurate and reproducible results, empowering you to make groundbreaking discoveries.
Item#:
ASPR1MASHTES384FULL

PR1MA™-Shell Plates - 96-Well, Full Skirted


Discover the next generation of PCR plates: Rugged Polycarbonate Frame + Polypropylene Wells

 

Engineered for unparalleled durability, PR1MA™-Shell PCR Plates are designed to accommodate all laboratory workflows and are ideal for use on automated liquid handling platforms.

 

PR1MA™-Shell sets a new standard in performance and convenience.

 

·       The polycarbonate frame is rigid and flat to facilitate a firm grip by robotic arms and ensure reliable placement

·       Polypropylene wells provide optimal thermal transfer and higher reaction efficiency

·       White well options enhance the fluorescence signal during qPCR

·       Wells are slightly raised to ensure evaporation-proof sealing with film, foil, or strip caps

·       ANSI/SBS footprint and well spacing

·       Vibrant colors offer a visual distinction between different samples or experiments

·       Certified free of detectable RNase, DNase, DNA, PCR inhibitors, and tested pyrogen-free

 

 

Trust MIDSCI™ for PCR consumables that deliver accurate and reproducible results, empowering you to make groundbreaking discoveries.
Item#:
ASPR1MASHTES96FULL

PR1MA™-Shell Plates - 96-Well, Semi Skirted


Discover the next generation of PCR plates: Rugged Polycarbonate Frame + Polypropylene Wells

 

Engineered for unparalleled durability, PR1MA™-Shell PCR Plates are designed to accommodate all laboratory workflows and are ideal for use on automated liquid handling platforms.

 

PR1MA™-Shell sets a new standard in performance and convenience.

 

·       The polycarbonate frame is rigid and flat to facilitate a firm grip by robotic arms and ensure reliable placement

·       Polypropylene wells provide optimal thermal transfer and higher reaction efficiency

·       White well options enhance the fluorescence signal during qPCR

·       Wells are slightly raised to ensure evaporation-proof sealing with film, foil, or strip caps

·       ANSI/SBS footprint and well spacing

·       Vibrant colors offer a visual distinction between different samples or experiments

·       Certified free of detectable RNase, DNase, DNA, PCR inhibitors, and tested pyrogen-free

 

 

Trust MIDSCI™ for PCR consumables that deliver accurate and reproducible results, empowering you to make groundbreaking discoveries.
Item#:
ASPR1MASHTES96SEMI

PR1MA™ Untreated Multi-Well Plates

  • Available in 6-, 12-, 24-, 48-, and 96-well format
  • Polystyrene, NOT Tissue Culture Treated (suspension culture)
  • Sterile (gamma irradiated)
  • Plates are labeled with alpha-numeric markings for easy identification of individual wells
  • Each well has a raised rim that nests into the rings on the single-direction lid to reduce evaporation and limit the risk of cross-contamination
  • Lids have a stacking feature for added stability when handling tall stacks of plates 
Item#:
ASCHROPLATES1


Need an order expedited? Contact your sales rep or email Tech@midsci.com

Looking for mats? Click here! Looking for sealing film? Click here!


 

Deep Well Plates and Combs for KingFisher Systems 

FEATURING:

Ultra-flat surfaces
Alphanumeric grid reference
Raised Well Design - improved heat sealing and reduced cross contamination
Storage down to -80°C
Automation compliant footprint (SBS/ ANSI/SLAS specification)
Made from Virgin Polypropylene; no mold release agents or regrind
RNase, DNase, DNA, and Pyrogen Free Certification

MID-KF05US-9-N

Our 250 ul 96-well elution plate is ideal for magnetic bead-based nucleic acid and protein affinity purification performed on automated separation systems.  The unique square top with round bottom design allows for optimum mixing and enhanced recovery of magnetic beads.  Wells also have a conical V-shaped bottom to help concentrate precipitates.

Maximum Volume:         350 ul
Working Volume:            250 ul
Volume with Mat:          250 ul
Matching silicone cap mats: MID-MATS-9-PRT, MID-MATS-9-BULK, MID-SQ-9-PRT

 

MID-KF10US-24-N

Our 5.0 mL 24-well plate is ideal for magnetic bead-based nucleic acid and protein affinity purification (of larger sample sizes, 5 mL) performed on automated separation systems.  The unique square top with round bottom design allows for optimum mixing and enhanced recovery of magnetic beads.  Wells also have a conical V-shaped bottom to help concentrate precipitates.

Maximum Volume:         10.0 mL
Working Volume:            5.0 mL
Volume with Mat:          5.0 mL
Matching silicone cap mats: MID-MATS-24-N

 

MID-KF22US-9-N

Our 2.0 mL 96-well plate is ideal for magnetic bead-based nucleic acid and protein affinity purification performed on automated separation systems.  The unique square top with round bottom design allows for optimum mixing and enhanced recovery of magnetic beads.  Wells also have a conical V-shaped bottom to help concentrate precipitates.

Maximum Volume:         2.0mL
Working Volume:            1.0mL
Volume with Mat:          1.0mL

Matching silicone cap mats: MID-MATS-9-PRT, MID-MATS-9-BULK, MID-SQ-9-PRT
ladder, 10-180 kDa, 2x250ul

Bullseye Pre-stained Protein Ladder, 10-180 kDa

  • Ready to use - no boiling necessary
  • Stable at ambient temperature
  • 2 reference bands - red at ~75kDa and green at ~25kDa
  • Use 5-15µl, depending on the size of the wells
  • No freeze-thaw involved
  • Broad range ladder

Application: The Bullseye Pre-stained Protein Ladder is suitable for visualizing proteins during electrophoresis without staining and for monitoring transfer onto membrane.

The Bullseye Pre-stained Protein Ladder contains 10 prestained proteins, covering a wide range of molecular weights from 10 to 180kDa. It is designed for monitoring protein separated during polyacrylamide gel electrophoresis, verification of Western transfer efficiency on membranes and for sizing of proteins. The ladder is supplied in protein gel loading buffer and is ready-to-use. No need to heat, dilute, or add reducing agent before loading. One prominent feature of this protein ladder, is that it remains in the liquid state at the storage temperature; therefore, there is not a freeze-thaw cycle involved, making the ladder very stable.


Contents:
0.1~0.4 mg/ml of each protein in 20 mM Trisphosphate (pH 7.5 at 25°C), 2% SDS, 1 mM reducing reagent, 3.6 M Urea, and stabilizer.

Usage:
For each application, 5-15µl is needed, depending on the size of the wells.

Quality Control:
Tested in polyacrylamide gel electrophoresis and Western Blotting.

Storage: -20 °C.

Note for image:

The apparent molecular weight of each protein (kDa) has been determined by calibration against an unstained protein ladder in each electrophoresis condition.
Item#:
BEPAR
Your Price:
167.00
Each

Autoradiography Cassette Aluminum 5x7

Classic Push-Button Cassettes

  • Constructed From Lightweight Aluminum & Nylon
  • Special Curved Door Gives Exceptional Screen-Film Contact
Item#:
ASCASSETTES

RunBlue gels are more rigid and up to 10 times stronger than conventional precast gels resulting in more robust gels that can be handled with confidence. No tearing - an 8% gel can be thrown into the air and caught without breaking!

  • RunBlue gels are manufactured using a unique method of polymerisation that results in more uniform and more complete polymerisation of the Acrylamide when compared to conventional, chemical based methods. This results in gels that have superior batch to batch reproducibility and practically eliminates exposure to un-polymerised Acrylamide.
  • RunBlue gels incorporate a stacking gel that has been dyed (red for SDS) for the simplest ever sample loading. In addition, there is no comb to remove as the top of the teeth are fixed in place. This not only prevents the teeth from being accidentally torn off during comb removal but that all of the wells are uniform, with the teeth remaining vertical. This prevents sample cross contamination / inability to load desired Vol. The teeth also protrude above the lower front plate of the gel cassette preventing sample flow over into adjacent wells.
  • Runblue gels are available in two different cassette sizes providing compatibility with the most common gel tanks and apparatus.  10x10 cm cassettes are compatible with Invitrogen/Life Technologies equipment and any other equipment designed for this format, such as the Hoeffer and Owl tanks. Our 8x10 cm cassette is designed to fit the Biorad equipment (Mini Protean I, II and II, Tetra etc).
  • The resolving gel on RunBlue gels extends to the very bottom of the cassette which not only provides improved resolution but also there is no protruding gel that requires trimming prior to blotting. There is also no additional tape / covers to remove - simply take the gel from the pack and use.
  • RunBlue gels are supplied in boxes of 10 gels. They are available in a 12 well format, covering a range of concentrations and gradients.

Benefits of RunBlue Precast Gels

  • 2 year shelf life - never throw away another gel
  • Up to 10x stronger - no more torn, ruined gels
  • Load up to 20µl - see everything in your sample
  • Dyed stacking gel - makes gel loading child's play (SDS = Red)
  • No comb to remove - no more broken or bent teeth
  • Full length resolving gel - improved resolution and better blotting
  • 10x10 cm and 8x10 cm cassette - total compatibility with most popular tanks including Invitrogen and Biorad equipment
  • Fast delivery with immediate and expert technical support for total peace of mind
  • Best value gels available - save money!
Item#:
ASPRECASTGBIOR

MAXI Flex Tube Kit

MAXI Flex Tubes combine two modes of action: electro-elution of nucleic acid molecules from polyacrylamide or agarose gels and dialysis or buffer exchange of protein molecules. Flex Tubes allow rapid, secure, simple loading and recovery, with high performance as the most convenient, user friendly, electro-elution and dialysis system on the market.

KIT CONTENTS

  • Flex Tubes 2/10/30/50/ 100 pieces
  • Supporting tray (for electro elution protocol) 1ea. (select kits)
  • Floating rack (for dialysis protocol) 1ea. (select kits)
  • Information and Protocol Manual 1ea.

SPECIFICATIONS

  • Membrane cut-off: 3.5K(11bp), 6-8K(18-24bp), 12-14K(36-42bp), 25K(76bp) or 50K(152bp) MWCO
  • Tube volume: 3ml
  • Dialysis volume: 0.1-3ml
  • Min. sample size for extraction: 20µg
  • Max. gel slice: 2cm x 1cm
  • Membrane ultra-clean, sulfur and heavy metal free. EDTA treated
  • Flex Tube MWCO are in kilo Daltons (K) for proteins and corresponding base pairs (bp) for nucleic acids as indicated in the table below:

kilo Daltons

base pairs

1K

3 bp

3.5K

11bp

6-8K

18-24bp

12-14K

36-42bp

25K

76bp

50K

152bp

 

APPLICATIONS

  • Dialysis, electro-elution or buffer exchange with volumes between 0.1-3ml
  • Preparation of protein samples for MALDI-MS
  • Sample concentration
  • Large-scale protein dialysis, such as antibodies and recombinant protein purification
  • Removal of contaminating micro-molecules
  • Tissue culture extraction purification
  • Removal of salts, surfactants, solvents, and detergents
  • Complex formation studies (protein-protein, protein-DNA, and protein-RNA)
  • pH and buffer adjustment of sample solutions, protein extraction or cell extraction
  • High throughput dialysis
  • Peptide dialysis, as small as 10 amino acids
  • Virus-particles purification
Item#:
ASISOLAION2

MIDI Flex Tube Kit

MIDI Flex Tubes combine two modes of action: electro-elution of nucleic acid molecules from polyacrylamide or agarose gels and dialysis or buffer exchange of protein molecules. Flex Tubes allow rapid, secure, simple loading and recovery, with high performance as the most convenient, user friendly, electro-elution and dialysis system on the market.

KIT CONTENTS

  • Flex Tubes 2/10/30/50/ 100 pieces
  • Supporting tray (for electro elution protocol) 1ea. (select kits)
  • Floating rack (for dialysis protocol) 1ea. (select kits)
  • Information and Protocol Manual 1ea.

SPECIFICATIONS

  • Membrane cut-off: 1K(3bp), 3.5K(11bp) or 6-8K(18-24bp) MWCO
  • Tube volume: 800µl
  • Dialysis volume: 50-800µl
  • Min. sample size for extraction: 0.5µg
  • Max. gel slice: 1cm x 0.5cm
  • Membrane ultra-clean, sulfur and heavy metal free. EDTA treated
  • Flex Tube MWCO are in kilo Daltons (K) for proteins and corresponding base pairs (bp) for nucleic acids as indicated in the table below:

kilo Daltons

base pairs

1K

3 bp

3.5K

11bp

6-8K

18-24bp

12-14K

36-42bp

25K

76bp

50K

152bp

 

APPLICATIONS

  • Dialysis, electro-elution or buffer exchange with volumes between 50-800µl
  • Preparation of protein samples for MALDI-MS
  • Sample concentration
  • Large-scale protein dialysis, such as antibodies and recombinant protein purification
  • Removal of contaminating micro-molecules
  • Tissue culture extraction purification
  • Removal of salts, surfactants, solvents, and detergents
  • Complex formation studies (protein-protein, protein-DNA, and protein-RNA)
  • pH and buffer adjustment of sample solutions, protein extraction or cell extraction
  • High throughput dialysis
  • Peptide dialysis, as small as 10 amino acids
  • Virus-particles purification.
Item#:
ASISOLAION4
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