PCR/qPCR

MIDSCI can supply almost every needed product for PCR/qPCR: tubes, plates, strips, caps, taq, Mastermixes, dNPTs, dye and probe for qPCR, sealing film and much more.  If you are looking for any of these things, we can match almost all needs to all cyclers.  If you don’t find it easily contact us at tech@midsci.com and we will find it for you. 

96 or 384 we have it.  Non-Skirted, semi-skirted or full skirted, we have it.  0.1, 0.2, or 0.5 mL we have it.  A1, A12, H12, A24, P24 cuts, we have them.  Sybr© Green or probe alternative, we have it.  

Pro Tip: Colored plastics do not have any impact on DNA amplification.  But when setting up qPCR it is often recommended to use white plastics.  This will limit or prevent fluorescence refraction.  Light refraction can minimize sensitivity and consistency. 

Pro Tip 2: If you are seeing inconsistencies in PCR/qPCR try PR1MA Pipette Tips.  This will help optimize and reduce variance in the reactions.  Simple test, see the residual liquid left in tips?  This means some of the taq, dNTPs, sample, etc is left in the tip.  PR1MA tips help reduce that significantly and improve your consistency. 

Pro Tip 3: Seeing evaporation in the outer wells of a PCR/qPCR plate?  Sometimes it’s the plate, sometimes it the seal, and sometimes it’s the cycler.  Call us we have solutions.

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Semi-Dry Blotting Systems, 20cm x 20cm

Choose Galileo Insight™ semi-dry sytems for rapid and efficient molecular transfer.
Galileo semi-dry electroblotters provide quick and efficient transfer of nucleic acids and proteins from agarose or acrylamide gels. Since only the tranfer "sandwich" of gel, membrane and blotting papers must be kept wet, much less buffer is required than traditional tank transfer systems. Solid plate style electrodes (stainless steel cathode and platinum anode) assure even and complete molecular transfer.

The larger format 2020 system is ideal for transfer of larger gels or simultaneous transfer of multiple smaller gels. System is supplied complete with power leads and a sample pack of our ultra-pure cotton fiber filter paper.
Item#:
ASSEMIELECT2020
Polyolefin, 2.8 mil, Ultra-

These optically clear polyester films are ideally suited for Real Time PCR (qPCR). They feature an inert, ultra-clear, and strong encapsulated silicone adhesive that provides extraordinary performance in real-time PCR applications.

Encapsulated silicone greatly reduces the risk of sample contamination due to exposure to adhesive. Once the film is pressed onto the plate, the raised portion of the well penetrates the encapsulation and a tight seal is formed on just that surface (not over the sample). This silicone seal adheres aggressively and diminishes the possibility of "Edge Effect".

The plasticized backing of this film is scored on both ends to allow for two handed (precise) placement on the plate. Both film and adhesive maintain incredible clarity and are the choice for highly demanding manual and automated processes.

Certified free of RNase, DNase, Pyrogen, and nucleic-acid contamination.

Length

141.27 mm

Length (w/ end tabs removed)

117.5 mm

Width

77.8 mm

Overall Thickness

2.8 mil

Adhesive Thickness

2.0 mil

Material

Polyester

Adhesive

Encapsulated Silicone

Max. Temperature

120 °C

Min. Temperature

-40 °C

Primary Usages

Real Time PCR (qPCR), PCR

Item#:
MID-UC500
Your Price:
1114.00
Each

The polyester films are a great general purpose seal; ideal for sample protection and elimination of well-to-well sample cross contamination. The acrylic adhesive offers a strong bond that seals tightly on polypropylene and polystyrene plates.

This seal is ideally suited for short term storage, Elisa assays, prevention of sample loss, and general lab usage.

The paper backing of this film is scored on both ends to allow for two handed (precise) placement on the plate.

For PCR applications see our standard PCR film or our Real Time PCR film.

For applications where accessing samples through the seal with pipet tips or a probe is required, see the pierceable aluminum (MID-PCR-200) film.

Certified free of RNase, DNase, Pyrogen, and nucleic-acid contamination.

Length

146.05 mm

Length (w/ end tabs removed)

119.05 mm

Width

79.38 mm

Overall Thickness

3.1 mil

Adhesive Thickness

1.1 mil

Material

Polyester

Adhesive

Medical Grade Acrylic

Max. Temperature

120 °C

Min. Temperature

-40 °C

Primary Usages

Elisa, Storage or Sample Cover

Item#:
MID-PCR-TS
Your Price:
195.00
Each
This item is discontinued
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