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PCR/qPCR
MIDSCI can supply almost every needed product for PCR/qPCR: tubes, plates, strips, caps, taq, Mastermixes, dNPTs, dye and probe for qPCR, sealing film and much more. If you are looking for any of these things, we can match almost all needs to all cyclers. If you don’t find it easily contact us at tech@midsci.com and we will find it for you.
96 or 384 we have it. Non-Skirted, semi-skirted or full skirted, we have it. 0.1, 0.2, or 0.5 mL we have it. A1, A12, H12, A24, P24 cuts, we have them. Sybr© Green or probe alternative, we have it.
Pro Tip: Colored plastics do not have any impact on DNA amplification. But when setting up qPCR it is often recommended to use white plastics. This will limit or prevent fluorescence refraction. Light refraction can minimize sensitivity and consistency.
Pro Tip 2: If you are seeing inconsistencies in PCR/qPCR try PR1MA Pipette Tips. This will help optimize and reduce variance in the reactions. Simple test, see the residual liquid left in tips? This means some of the taq, dNTPs, sample, etc is left in the tip. PR1MA tips help reduce that significantly and improve your consistency.
Pro Tip 3: Seeing evaporation in the outer wells of a PCR/qPCR plate? Sometimes it’s the plate, sometimes it the seal, and sometimes it’s the cycler. Call us we have solutions.
The Rotocycler 72 facilitates the handling and insertion of caps on the tubes by offering a special solid aluminum loading rack. This rack can hold up to 18 x 0.1 mL tube strips for a total of 72 tubes. Other cavities can hold larger reaction tubes. To keep reactions cool during setup, simply place the rack in a refrigerated area. For easy reference, all wells are numbered. Color coding is made possible by inserting a Capinsert in up to two locations on the rack.
Includes: Rotocycler 72 and 500 assorted color coding inserts
Accessories for the Rotocycler 72
T319-4N |
Rotocycler 0.1 mL Tube and Cap Strips for Qiagen Rotor-Gene Q |
T319-72D2 |
Rotocycler 72 Rotor with 100 µL wells for Qiagen Rotor-Gene Q |
T319-100D1 |
Rotocycler 100 Rotor with 25 µL wells for Qiagen Rotor-Gene Q |
The RotoCycler Discs are specially made to be used with the Qiagen Rotor-Gene Q Real-Time Rotary Analyzer. Two models are available: a 72-well format with 100 µL tubes and a 100-well format for reactions up to 25 µL. The discs a"one-piece "plate" equivalent, having vertically oriented wells compatible with automated reaction setup using a robotic liquid handling system.
- The Extractor removes over 99% of the ethidium bromide from gel staining solutions quickly and easily.
- Each device can decontaminate up to 10 liters of gel staining solution
The RotoCycler Discs are specially made to be used with the Qiagen Rotor-Gene Q Real-Time Rotary Analyzer. Two models are available: a 72-well format with 100 µL tubes and a 100-well format for reactions up to 25 µL. The discs a"one-piece "plate" equivalent, having vertically oriented wells compatible with automated reaction setup using a robotic liquid handling system.
- Selection of each wavelength is by an easy turn of the dial located on one end of the housing
- The uniquely designed reflector surfaces mounted behind each tube provides maximum UV for fluorescence applications
- Lightweight and ergonomically designed for hand or stationary use
- 1 year warranty
Dimensions
3UV-34: 9.5L x 3W x 4.5D in. (241 x 76 x 114mm)
3UV-36: 12.5L x 3W x 4.5D in. (318 x 76 x 114mm)
3UV-38: 15.5L x 3W x 4.5D in. (394 x 76 x 114mm)
| 3UV Model | Part # 115V/60Hz |
Watts | Wavelengths |
| 3UV-34 | 95-0341-01 | 4 Watts | 254/302/365nm |
| 3UV-36 | 95-0342-01 | 6 Watts | 254/302/365nm |
| 3UV-38 | 95-0343-01 | 8 Watts | 254/302/365nm |
- Small footprint for benchtop use
- Preset and manual controls for ultraviolet or time exposures. Preset exposure delivers 120,000 microjoules or five minutes of exposure
- Microprocessor measures and control UV output, ensuring maximum energy efficiency
- Large LED display and touch panel which displays time or energy settings
- Window on the door allows viewing of the process yet blocks out the UV radiation
- Select from models with 302nm midrange UV, 365nm longwave UV or 254nm shortwave UV Interior: 12 deep x 10 wide x 5 high inches (305 x 254 x 127mm)
- Overhead UV supplies uniform UV
- Shortwave UV model is available with choice of door (CL-1000) or drawer (CX-2000) styles
- Built-in UV sensor is calibrated at the UVP factory to ensure accuracy and reliability
- Crosslinking for attaching nucleic acids to a membrane takes seconds as compared to oven baking
CL-1000 Dimensions: Exterior: 13.7 deep x 15.6 wide x 8.75 high inches (248 x 396 x 222mm)
CX-2000 Dimensions:
Exterior: 17.5"W x 15"D x 9.75"H (445 x 381 x 248mm)
Interior: 11.4"W x 11.3"D x 3.6"H (290 x 287 x 91mm)
Depth of sample tray 11.25 Width of sample tray 11.5
Sample surface to lamp approximately 3.5 in. (89mm)
1 year warranty
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- Lamps are lightweight.
- Models available include shortwave, longwave UV or a combination shortwave/longwave version
- Combination version comes with a snap-on wavelength selector for switching between wavelengths
- Use these lamps with the J-129 Lamp Stand (pictured) or the C-10 Cabinet
- 1 year warranty
Handheld Lamp Dimensions:
14.9"L x 3.2"W x 2.5"H
Lamp Stand Dimensions: 9.5"H x 13"L
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