IBI DNA/RNA/Protein Extraction Kits

• Sample Size: up to 5×106 cultured animal cells/up to 25mg animal tissue /up to 500µl whole blood/up to 200µl biological fluids (serum, plasma)
• Expectant Yield: up to 9µg of genomic DNA/20µg of total RNA/120µg of protein from 1×106 of HeLa cells
• Operation Time: DNA/RNA purification within 25 minutes, protein precipitation within 50 minutes
• Elution Volume: 50  200µl (genomic DNA)/25  50µl (total RNA)
• Format: genomic DNA spin column and total RNA spin column

The IBI All Prep DNA RNA Protein Mini Kit provides an efficient method for purifying genomic DNA, total RNA, and total protein simultaneously from cultured cells, animal tissues, whole blood, and biological fluids. Chaotropic salt is used to lyse cells and inactivate DNases and RNases, allowing DNA to bind to the genomic DNA spin column. The flow-through can then be transferred to the RNA Spin column for RNA binding. Contaminants are effectively removed using wash buffers followed by pure genomic DNA elution in a low salt buffer and pure total RNA elution in RNase-free water.

DNA/RNA purification can be completed in 25 minutes without phenol/chloroform extraction or alcohol precipitation, and protein purification can be completed in 50 minutes. The purified DNA, with approximately 20  30Kb, is suitable for use in PCR or other enzymatic reactions and the purified RNA (including miRNA) is ready for use in RT-PCR, Real-Time PCR, northern blotting, primer extension, mRNA selection, and cDNA synthesis. The purified proteins can be directly analyzed on a SDS-PAGE and subsequent western blot.

The quality of the All Prep DNA RNA Protein Mini Kit is tested on a lot-to-lot basis by isolating genomic DNA and total RNA from 1x106 cultured animal cells. The purified DNA and total RNA is quantified with a spectrophotometer and analyzed by electrophoresis on a 1% agarose gel.  The purified protein is quantified by Bradford assay analyzed on SDS-PAGE.

Specifications

Sample: Cultured cells, animal tissue, whole blood & biological fluids
Format: Genomic DNA spin column and Total RNA spin column.
Yield: up to 9ug of Genomic DNA / 20ug of Total RNA / 120ug of Protein from 1 x 106 HeLa cells.
Operation Time: Within 25 minutes
Elution volume: 50  200ul for Genomic DNA / 25  50ul for Total RNA.
Simultaneous extraction of Genomic DNA and Total RNA from cultured cells, animal tissues, whole blood and biological fluids.

• Sample: Cultured cells
• Yield: High yield, High quality DNA (A260/A280 = 1.8-2.0)
• Format: Scalable DNA precipitation method
• Kit Storage: Dry at room temperature (15-25°C) for up to 2 years, RNase A should be stored at 4°C for extended periods

The gPURE DNA Isolation Kit offers a simple and gentle reagent DNA precipitation method for isolating high molecular weight genomic, mitochondrial or viral DNA suitable for archiving or sensitive downstream applications. This highly versatile solution based system can be scaled proportionately in order to satisfy larger sample volumes providing a convenient sample-storage procedure with minimal hands on time. Initially cells are lysed in the presence of detergents and a proprietary DNA stabilization solution followed by RNase A treatment. Once proteins and other contaminants are removed DNA is precipitated then rehydrated. The high quality extracted DNA is ready for use in a variety of downstream applications.

Quality Control
gPURE DNA Isolation Kits are tested on a lot-to-lot basis by isolating DNA from (3-5 x 106) cultured cells. The isolated DNA (5-15 μg with an A260/A280 ratio of 1.8â2.0) is quantified with a spectrophotometer and analyzed by electrophoresis.

Components and Storage
Cell Lysis Buffer, Protein Removal Buffer, DNA Hydration Buffer should be stored dry at room
temperature (15-25°C) for up to 1 year. RNase A should be stored at 4°C for extended periods.

• Sample: up to 25 mg of tissue, up to 25 mg of paraffin-embedded tissue
• Yield: 5-30 µg
• Format: Spin column
• Operation time: Within 20 minutes

Introduction
The Total RNA Mini Kit (Tissue) was designed specifically for purifying total RNA from a variety of animal and paraffin-embedded tissue. Tissue samples can be efficiently homogenized in a microcentrifuge tube using the provided micropestle. Detergents and chaotropic salt are used to lyse cells and inactivate RNase and optional DNase treatments can be followed to remove unwanted DNA residue. RNA in the chaotropic salt is bound by the glass fiber matrix of the spin column (1). Once any contaminants have been removed, using the Wash Buffer (containing ethanol), the purified total RNA is eluted by RNase-Free Water, and is ready for use in RT-PCR, Northern Blotting, Primer Extension and cDNA Library Construction. Phenol extraction or alcohol precipitation is not required.

Quality Control 
The quality of the Total RNA Mini Kit (Tissue) is tested on a lot-to-lot basis by isolating total RNA from a 25 mg animal tissue sample. The purified RNA is quantified with a spectrophotometer and checked by electrophoresis.

*Add absolute ethanol (see the bottle label for volume) to the Wash Buffer prior to initial use.

Caution 
RB Buffer contains chaotropic salt which is a harmful irritant. During operation, always wear a lab coat, disposable gloves, protective goggles, and (anti-fog) procedure mask.

Introduction
IBI Isolate is a phenol, chloroform and guanidine isothiocyanate based scalable solution for extracting high-quality total RNA as well as simultaneous extraction of RNA, DNA and protein from a wide variety of samples such as blood, buffy coat, plasma, serum, cultured cells and tissue. The extracted RNA can be used directly in a variety of downstream applications such as cDNA Library Construction, Cloning, RT-PCR (Endpoint), Real-Time PCR, Nuclease Protection Assays and Northern Blotting.

Quality Control
IBI Isolate is tested on a lot-to-lot basis. RNA from a 1 ml human blood sample is extracted using IBI Isolate. 10 µL from a 50 µL eluate of RNA is analyzed by electrophoresis on a 0.8% agarose gel.

Advantages
 Extract total RNA or simultaneous RNA, DNA and protein within 1 hour
 Sample: up to 300 µL (blood, buffy coat, serum, plasma), up to 5 x 106 (cultured cells), 50-100 mg (tissue)
 Scalable
 Format: phenol, chloroform and guanidine isothiocyanate

Applications
cDNA Library Construction, Cloning, RT-PCR (Endpoint), Real-Time PCR, Nuclease Protection Assays and Northern Blotting

Caution
IBI Isolate contains phenol and guanidine isothiocyanate. During operation, always work in a fume hood, always wear a lab coat, disposable gloves, protective goggles and (anti-fog) procedure mask. Disposable/non-disposable glassware, plasticware and automatic pipettes should be sterile (RNase-free) and used only for RNA procedures.

Additional Requirements
RNA Extraction: chloroform, isopropanol, 70% ethanol, RNase-free Water, 1.5 mL microcentrifuge tubes (RNase-free) DNA Extraction: chloroform, absolute ethanol, 70% ethanol, sodium citrate/ethanol solution (0.1 M sodium citrate in 10% ethanol, pH 8.5), 8 mM NaOH solution or TE Buffer pH 8.5, 1.5 mL microcentrifuge tubes

Components and Storage
IBI Isolate is shipped at room temperature and can be stored dry at 2°C to 25°C for up to 9 months.

The 96-Well Genomic Plant DNA Kit provides an efficient method for isolating total DNA (genomic, mitochondrial, and chloroplast DNA) from plant tissue and cells. Samples are initially disrupted by grinding in liquid nitrogen, followed by lysate treatment with RNase A. The unique GR Buffer is able to lyse most common plant samples and also samples high in polysaccharides. DNA phenol extraction is not required and the entire procedure can be completed in 1.5 hours. The isolated total DNA is ready for use in PCR, Real-time PCR, Southern Blotting, mapping, and RFLP.

MINI Flex Tube Kit

MINI Flex Tubes combine two modes of action: electro-elution of nucleic acid molecules from polyacrylamide or agarose gels and dialysis or buffer exchange of protein molecules. Flex Tubes allow rapid, secure, simple loading and recovery, with high performance as the most convenient, user friendly, electro-elution and dialysis system on the market.

KIT CONTENTS

• Flex Tubes 2/10/30/50/ 100 pieces
• Supporting tray (for electro elution protocol) 1ea. (select kits)
• Floating rack (for dialysis protocol) 1ea. (select kits)
• Information and Protocol Manual 1ea.

SPECIFICATIONS

• Membrane cut-off: 6-8K(18-24bp), 12-14K(36-42bp) or 25K(76bp) MWCO
• Tube volume: 250µl
• Dialysis volume: 10-250µl
• Min. sample size for extraction: 0.5µg
• Max. gel slice: 0.4cm x 1.1cm
• Membrane ultra-clean, sulfur and heavy metal free. EDTA treated
• Flex Tube MWCO are in kilo Daltons (K) for proteins and corresponding base pairs (bp) for nucleic acids as indicated in the table below:

APPLICATIONS

• Dialysis, electro-elution or buffer exchange with volumes between 10-250µl
• Preparation of protein samples for MALDI-MS
• Sample concentration
• Large-scale protein dialysis, such as antibodies and recombinant protein purification
• Removal of contaminating micro-molecules
• Tissue culture extraction purification
• Removal of salts, surfactants, solvents, and detergents
• Complex formation studies (protein-protein, protein-DNA, and protein-RNA)
• pH and buffer adjustment of sample solutions, protein extraction or cell extraction
• High throughput dialysis
• Peptide dialysis, as small as 10 amino acids
• Virus-particles purification

• Sample Size: 250-500 mg of soil
• Expectant Yield: up to 5 µg of genomic DNA
• Format: beadbeating tubes, PCR inhibitor removal columns and genomic DNA spin columns
• Operation Time: within 40 minutes
• Elution Volume: 30-100 µl
• Kit Storage: dry at room temperature (15-25°C) for up to 18 months without showing any reduction in performance

The Soil DNA Extraction Kit was designed for rapid isolation of genomic DNA from microorganisms such as bacteria, archaea, fungi, and algae in soil samples. The soil sample is homogenized and disrupted using SL1 lysis Buffer combined with ceramic beads. Insoluble particles, proteins and PCR inhibitors such as humic acid are then precipitated with a unique inhibitor removal Buffer (SL2). In addition, residual PCR inhibitors remaining in the clear supernatant are further removed by passing through a specialized PCR Inhibitor Removal Column. The flow-through is then mixed with a binding buffer (SL3) and the genomic DNA is bound by the GD Column. The column is then washed and the DNA is eluted with Elution Buffer. DNA phenol extraction or alcohol precipitation is not required and the entire procedure can be completed within 40 minutes. The purified genomic DNA is ready for use in PCR, restriction enzyme digestion, and sequencing reactions.

Introduction
IBI Plant Isolate provides a quick and easy 3 step CTAB and chloroform based method to isolate total DNA (including genomic, mitochondrial and chloroplast DNA) from a variety of plant species (including algae and cyanobacteria). This unique reagent is able to lyse most common plant samples and plant samples with high a polysaccharide content. The extracted DNA is suitable for routine PCR screening, Real-Time PCR, Southern Blotting, Mapping and RFLP. Phenol extraction is not required and the entire procedure can be completed within 50 minutes.

Quality Control
IBI Plant Isolate is tested on a lot-to-lot. 50 mg of fresh Arabidopsis leaves are initially ground in IBI Plant Isolate. A 15 µL aliquot of extracted genomic DNA from a µL eluate is analyzed by electrophoresis on a 1% agarose gel.

Advantages

• High molecular weight genomic DNA extraction from a variety of plant species 
  
• Sample: up to 1 g of fresh plant tissue and up to 0.5 g of dry plant tissue
  
• Scalable, simple and gentle CTAB and chloroform based DNA precipitation method
  
• Cost effective

  Applications
  PCR, Real-Time PCR, Southern Blotting, Mapping and RFLP

  Caution
  IBI Plant Isolate contains irritants. During operation, always wear a lab coat, disposable gloves, protective goggles and (anti-fog) procedure mask.

  Additional Requirements
  Mortar and pestle, 1.5 mL microcentrifuge tubes or 15 mL centrifuge tubes, absolute ethanol for preparing 70% ethanol in water, chloroform, isopropanol, TE buffer or ddH2O

  Components and Storage

  Item	Product	Volume	RNase A (50mg/mL)	Shipping	Storage
  IBI Plant Isolate & RNase A	IB47610	4 mL	N/A	Room Temperature	Plant Isolate Dry at room temperature (15-25°C) for up to 1 year   RNase A 4°C for extended periods
  	IB47611	100 mL	50 µL
  	IB47612	200 mL	100 µL