The Genomic DNA Mini Kit for Plants provide a quick and easy method for purifying total DNA (including genomic DNA, mitochondrial and chloroplast DNA) from plant tissue. Samples are disrupted by both grinding in liquid nitrogen and lysis buffer incubation. The lysate is treated with RNase A to degrade RNA and then filtered to remove cell debris and salt precipitates. In the presence of the binding buffer, coupled with chaotropic salt, genomic DNA in the lysate binds to the glass fiber matrix of the spin column. Contaminants are removed using a Wash Buffer (containing ethanol) and the purified genomic DNA is eluted by a low salt Elution Buffer or TE. The procedure does not require DNA phenol extraction or alcohol precipitation, and can be completed in less than 1 hour. The purified genomic DNA is ready for use in PCR, Real-time PCR, Southern Blotting and RFLP.

The Genomic DNA Mini Kit for Tissue was designed specifically for purifying total DNA (including genomic, mitochondrial and viral DNA) from a variety of animal tissue, paraffin-embedded tissue, buccal swab and amniotic fluid. The provided micropestle can efficiently homogenize tissue samples to shorten the time in the Lysis Step. Proteinase K and chaotropic salt are used to lyse cells and degrade protein, allowing DNA to be easily bound by the glass fiber matrix of the spin column. Once any contaminants have been removed, using a Wash Buffer (containing ethanol), the purified DNA is eluted by a low salt Elution Buffer or TE. The entire procedure can be completed within 1 hour without phenol/chloroform extraction or alcohol precipitation. The expected yield of genomic DNA is up to 50µg and the purified DNA (with approximately 20-30 Kb) is suitable for use in PCR or other enzymatic reactions.

The Genomic DNA Kit for Blood or Cultured Cells provides an efficient method for purifying total DNA (including genomic, mitochondrial and viral DNA) from whole blood, frozen blood, buffy coat, cultured animal/bacterial cells and fungus. Chaotropic salt is used to lyse cells and degrade protein, allowing DNA to bind to the glass fiber matrix of the spin column. Contaminants are removed using a Wash Buffer (containing ethanol) and the purified genomic DNA is eluted by a low salt Elution Buffer or TE. The entire procedure can be completed in 1 hour without phenol/chloroform extraction or alcohol precipitation, with an average DNA yield of 6 µ g from 200 µ l of whole human blood and up to 50µ g of DNA from 200µ l of buffy coat. Purified DNA, with approximately 20-30 Kb, is suitable for use in PCR or other enzymatic reactions.