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Home > PCR, Protein, RNA, DNA > DNA and RNA Purification Kits > DNA and RNA Column Base Kits & Reagents > IBI gPURE Extraction Reagent Kits (Cultured Cells)

IBI gPURE Extraction Reagent Kits (Cultured Cells)

Item #: ASDNARNAKIT22

Sample: Cultured cells
Yield: High yield, High quality DNA (A260/A280 = 1.8-2.0)
Format: Scalable DNA precipitation method
Kit Storage: Dry at room temperature (15-25°C) for up to 2 years, RNase A should be stored at 4°C for extended periods

The gPURE DNA Isolation Kit offers a simple and gentle reagent DNA precipitation method for isolating high molecular weight genomic, mitochondrial or viral DNA suitable for archiving or sensitive downstream applications. This highly versatile solution based system can be scaled proportionately in order to satisfy larger sample volumes providing a convenient sample-storage procedure with minimal hands on time. Initially cells are lysed in the presence of detergents and a proprietary DNA stabilization solution followed by RNase A treatment. Once proteins and other contaminants are removed DNA is precipitated then rehydrated. The high quality extracted DNA is ready for use in a variety of downstream applications.

Quality Control
gPURE DNA Isolation Kits are tested on a lot-to-lot basis by isolating DNA from (3-5 x 106) cultured cells. The isolated DNA (5-15 Î¼g with an A260/A280 ratio of 1.8â2.0) is quantified with a spectrophotometer and analyzed by electrophoresis.

Components and Storage
Cell Lysis Buffer, Protein Removal Buffer, DNA Hydration Buffer should be stored dry at room
temperature (15-25°C) for up to 1 year. RNase A should be stored at 4°C for extended periods.

gPURE DNA Isolation Kit	IB47430	IB47431	IB47432
Number of Cells Processed Per Kit	2 x 10	6 x 10	6 x 10
Cell Lysis Buffer	3 mL	100 mL	1000 mL
Protein Removal Buffer	1 mL	40 mL	400 mL
DNA Hydration Buffer (10 mM Tris-HCl, 1mM EDTA, pH 8.0)	1 mL	50 mL	500 mL
RNase A (10 mg/mL)	25 µL	550 µL	5 mL

Cell Number	0.5-1 x 10	3-5 x 10	3.5 x 10
Tube Size	1.5 mL	1.5 mL	15 mL
Cell Lysis Buffer	150 µL	600 µL	6 mL
RNase A (10 mg/mL)	1 µL	3 µL	30 µL
Protein Removal Buffer	50 µL	200 µL	2 mL
Isopropanol	150 µL	600 µL	6 mL
70% Ethanol	150 µL	600 µL	6 mL
DNA Hydration Buffer	50 µL	100 µL	200 µL

PNRNADNA

Source Cell

Cultured Cell

Isolation type

DNA

AlphaPette™ by PR1MA™ Pipettors

PR1MA™ Low Retention Graduated Barrier Pipette Tips

SKU Number	# of Samples	Source Cell	Isolation type	Price	Quantity	Add to Cart
IB47430	1	Cultured Cell	DNA	30.4200 30.42 Each
IB47431	1	Cultured Cell	DNA	196.0600 196.06 Each
IB47432	1	Cultured Cell	DNA	1358.4900 1358.49 Each