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Home > PCR, Protein, RNA, DNA > DNA and RNA Purification Kits > DNA and RNA Column Base Kits & Reagents > IBI Total RNA Kits (Tissue)

IBI Total RNA Kits (Tissue)

Item #: ASDNARNAKIT21

Sample: up to 25 mg of tissue, up to 25 mg of paraffin-embedded tissue
Yield: 5-30 µg
Format: Spin column
Operation time: Within 20 minutes

Introduction
The Total RNA Mini Kit (Tissue) was designed specifically for purifying total RNA from a variety of animal and paraffin-embedded tissue. Tissue samples can be efficiently homogenized in a microcentrifuge tube using the provided micropestle. Detergents and chaotropic salt are used to lyse cells and inactivate RNase and optional DNase treatments can be followed to remove unwanted DNA residue. RNA in the chaotropic salt is bound by the glass fiber matrix of the spin column (1). Once any contaminants have been removed, using the Wash Buffer (containing ethanol), the purified total RNA is eluted by RNase-Free Water, and is ready for use in RT-PCR, Northern Blotting, Primer Extension and cDNA Library Construction. Phenol extraction or alcohol precipitation is not required.

Quality Control
The quality of the Total RNA Mini Kit (Tissue) is tested on a lot-to-lot basis by isolating total RNA from a 25 mg animal tissue sample. The purified RNA is quantified with a spectrophotometer and checked by electrophoresis.

Kit	IB47300	IB47301	IB47302	IB47303
RB Buffer	2 mL	30 mL	60 mL	130 mL
W1 Buffer	2 mL	30 mL	50 mL	130 mL
Wash Buffer* (Add EtOH)	1 mL (4 mL)	12.5 mL (50 mL)	25 mL (100 mL)	50 mL x 2 (200 mL x 2)
RNase-free H2O	1 mL	6 mL	15 mL	30 mL
RB Column	4 pcs	50 pcs	100 pcs	300 pcs
Filter Column	4 pcs	50 pcs	100 pcs	300 pcs
2 mL Collection Tubes	8 pcs	100 pcs	200 pcs	600 pcs
Micropestle	4 pcs	50 pcs	100 pcs	300 pcs

*Add absolute ethanol (see the bottle label for volume) to the Wash Buffer prior to initial use.

Caution
RB Buffer contains chaotropic salt which is a harmful irritant. During operation, always wear a lab coat, disposable gloves, protective goggles, and (anti-fog) procedure mask.

PNRNADNA

Source Cell

Lyse Cell

Isolation type

RNA

AlphaPette™ by PR1MA™ Pipettors

PR1MA™ Low Retention Graduated Barrier Pipette Tips

SKU Number	# of Samples	Source Cell	Isolation type	Price	Quantity	Add to Cart
IB47300	4	Lyse Cell	RNA	29.8200 29.82 Each
IB47301	50	Lyse Cell	RNA	307.4000 307.40 Each
IB47302	100	Lyse Cell	RNA	574.0100 574.01 Each
IB47303	300	Lyse Cell	RNA	1368.1800 1368.18 Each