• Sample Size: 250-500 mg of soil
• Expectant Yield: up to 5 µg of genomic DNA
• Format: beadbeating tubes, PCR inhibitor removal columns and genomic DNA spin columns
• Operation Time: within 40 minutes
• Elution Volume: 30-100 µl
• Kit Storage: dry at room temperature (15-25°C) for up to 18 months without showing any reduction in performance

The Soil DNA Extraction Kit was designed for rapid isolation of genomic DNA from microorganisms such as bacteria, archaea, fungi, and algae in soil samples. The soil sample is homogenized and disrupted using SL1 lysis Buffer combined with ceramic beads. Insoluble particles, proteins and PCR inhibitors such as humic acid are then precipitated with a unique inhibitor removal Buffer (SL2). In addition, residual PCR inhibitors remaining in the clear supernatant are further removed by passing through a specialized PCR Inhibitor Removal Column. The flow-through is then mixed with a binding buffer (SL3) and the genomic DNA is bound by the GD Column. The column is then washed and the DNA is eluted with Elution Buffer. DNA phenol extraction or alcohol precipitation is not required and the entire procedure can be completed within 40 minutes. The purified genomic DNA is ready for use in PCR, restriction enzyme digestion, and sequencing reactions.

Introduction
IBI Plant Isolate provides a quick and easy 3 step CTAB and chloroform based method to isolate total DNA (including genomic, mitochondrial and chloroplast DNA) from a variety of plant species (including algae and cyanobacteria). This unique reagent is able to lyse most common plant samples and plant samples with high a polysaccharide content. The extracted DNA is suitable for routine PCR screening, Real-Time PCR, Southern Blotting, Mapping and RFLP. Phenol extraction is not required and the entire procedure can be completed within 50 minutes.

Quality Control
IBI Plant Isolate is tested on a lot-to-lot. 50 mg of fresh Arabidopsis leaves are initially ground in IBI Plant Isolate. A 15 µL aliquot of extracted genomic DNA from a µL eluate is analyzed by electrophoresis on a 1% agarose gel.

Advantages

• High molecular weight genomic DNA extraction from a variety of plant species 
  
• Sample: up to 1 g of fresh plant tissue and up to 0.5 g of dry plant tissue
  
• Scalable, simple and gentle CTAB and chloroform based DNA precipitation method
  
• Cost effective

  Applications
  PCR, Real-Time PCR, Southern Blotting, Mapping and RFLP

  Caution
  IBI Plant Isolate contains irritants. During operation, always wear a lab coat, disposable gloves, protective goggles and (anti-fog) procedure mask.

  Additional Requirements
  Mortar and pestle, 1.5 mL microcentrifuge tubes or 15 mL centrifuge tubes, absolute ethanol for preparing 70% ethanol in water, chloroform, isopropanol, TE buffer or ddH2O

  Components and Storage

  Item	Product	Volume	RNase A (50mg/mL)	Shipping	Storage
  IBI Plant Isolate & RNase A	IB47610	4 mL	N/A	Room Temperature	Plant Isolate Dry at room temperature (15-25°C) for up to 1 year   RNase A 4°C for extended periods
  	IB47611	100 mL	50 µL
  	IB47612	200 mL	100 µL

MIDI Flex Tube Kit

MIDI Flex Tubes combine two modes of action: electro-elution of nucleic acid molecules from polyacrylamide or agarose gels and dialysis or buffer exchange of protein molecules. Flex Tubes allow rapid, secure, simple loading and recovery, with high performance as the most convenient, user friendly, electro-elution and dialysis system on the market.

KIT CONTENTS

• Flex Tubes 2/10/30/50/ 100 pieces
• Supporting tray (for electro elution protocol) 1ea. (select kits)
• Floating rack (for dialysis protocol) 1ea. (select kits)
• Information and Protocol Manual 1ea.

SPECIFICATIONS

• Membrane cut-off: 1K(3bp), 3.5K(11bp) or 6-8K(18-24bp) MWCO
• Tube volume: 800µl
• Dialysis volume: 50-800µl
• Min. sample size for extraction: 0.5µg
• Max. gel slice: 1cm x 0.5cm
• Membrane ultra-clean, sulfur and heavy metal free. EDTA treated
• Flex Tube MWCO are in kilo Daltons (K) for proteins and corresponding base pairs (bp) for nucleic acids as indicated in the table below:

APPLICATIONS

• Dialysis, electro-elution or buffer exchange with volumes between 50-800µl
• Preparation of protein samples for MALDI-MS
• Sample concentration
• Large-scale protein dialysis, such as antibodies and recombinant protein purification
• Removal of contaminating micro-molecules
• Tissue culture extraction purification
• Removal of salts, surfactants, solvents, and detergents
• Complex formation studies (protein-protein, protein-DNA, and protein-RNA)
• pH and buffer adjustment of sample solutions, protein extraction or cell extraction
• High throughput dialysis
• Peptide dialysis, as small as 10 amino acids
• Virus-particles purification.

MAXI Flex Tube Kit

MAXI Flex Tubes combine two modes of action: electro-elution of nucleic acid molecules from polyacrylamide or agarose gels and dialysis or buffer exchange of protein molecules. Flex Tubes allow rapid, secure, simple loading and recovery, with high performance as the most convenient, user friendly, electro-elution and dialysis system on the market.

KIT CONTENTS

• Flex Tubes 2/10/30/50/ 100 pieces
• Supporting tray (for electro elution protocol) 1ea. (select kits)
• Floating rack (for dialysis protocol) 1ea. (select kits)
• Information and Protocol Manual 1ea.

SPECIFICATIONS

• Membrane cut-off: 3.5K(11bp), 6-8K(18-24bp), 12-14K(36-42bp), 25K(76bp) or 50K(152bp) MWCO
• Tube volume: 3ml
• Dialysis volume: 0.1-3ml
• Min. sample size for extraction: 20µg
• Max. gel slice: 2cm x 1cm
• Membrane ultra-clean, sulfur and heavy metal free. EDTA treated
• Flex Tube MWCO are in kilo Daltons (K) for proteins and corresponding base pairs (bp) for nucleic acids as indicated in the table below:

PR1MA™ β - Agarase

PR1MA™ β - Agarase has a high tolerance to inhibitors in electrophoretic buffers such as TBE and TAE, eliminating the need to perform a buffer exchange step before digestion. PR1MA™ β - Agarase has higher thermal stability than other commercially available β - agarases, with a broad range of activity between 42°C and 50°C. Higher temperature digestion reduces the chances of residual agarose gelling at the end of the reaction, resulting in higher recovery of DNA or RNA.

Benefits

• Complete digestion of agarose, with no agarose fragments, left after the digestion
• Obtain DNA or RNA faster - no buffer exchange needed, with direct digestion in TAE or TBE
• Concentration: 1000 u / mL
• Operating temperature: 42°C to 50°C
• Recommended temperature: 50°C
• Storage temperature: -20°C 

Storage Buffer

• 50% glycerol
• 50 mM Tris-HCI
• 50 mM KCI
• 1 mM DTT
• 0.1  mM EDTA
• pH = 7.5 

*These products are intended for research use only, not for therapeutic or diagnostic purposes in humans or animals. The safety and efficacy of these products in diagnostic or other clinical uses has not been established.

The Total RNA MINI and MAXI kits (Plant) provide a simple and fast method to isolate total RNA from plant tissue and cells. Samples are ground in liquid nitrogen and filtered to remove debris. In the presence of a binding buffer and chaotropic salt, the total RNA in the lysate binds to the glass fiber matrix of the spin column. The optional DNase treatments can remove DNA residues and the contaminants can be washed with an ethanol based wash buffer. Finally, the purified total RNA is eluted by RNase-free water. This protocol does not require phenol extraction or alcohol precipitation, and the entire procedure can be completed within 60 minutes. The purified total RNA is ready for RT, RT-PCR, Real Time PCR and northern blotting.

• Sample: Gram (+) positive and Gram (-) negative bacterial cells
• Yield: Up to 60 µg of RNA - (1 x 10 Escherichia coli: 40-45 µg, 1 x 10 Bacillus subtilis: 50-55 µg)
• Convenient: Includes Lysozyme and Bacteria Lysis Buffer
• Format: RNA spin columns
• Operation Time: Within 30 minutes
• Elution Volume: 50-100 µL
• Kit Storage: Dry at room temperature (15-25°C) for up to 9 months, Lysozyme should be stored at -20°C for extended periods

The rBAC Mini RNA Bacteria Kit was designed for total RNA purification from Gram (-) negative and Gram (+) positive bacteria. The provided Lysozyme and Bacteria Lysis Buffer will efficiently lyse bacterial cell walls consisting of the peptidoglycan layer. Detergents and chaotropic salt are used to further lyse cells and inactivate RNase while RNA is bound by the glass fiber matrix of the RNA spin column. Once any contaminants have been removed, using the Wash Buffer (containing ethanol), the purified total RNA is eluted by RNase-free Water and is ready for use in a variety of subsequent reactions.

Quality Control
The quality of the rBAC Mini RNA Bacteria Kit is tested on a lot-to-lot basis by isolating RNA from Escherichia coli (1x10) culture (OD600=1.3, 1 mL) harvested by centrifugation at 16,000 x g for 1 minute. 10 µL from a 50 µL eluate of purified RNA is analyzed by electrophoresis on a 0.8% agarose gel.

*Lysozyme should be stored at -20°C for extended periods. Add Lysozyme to Bacteria Lysis Buffer immediately prior to use. Once Lysozyme is mixed with Bacteria Lysis Buffer, the solution can be stored for 2 weeks at 4°C.

**Add absolute ethanol (see the bottle label for volume) to Wash Buffer then mix by shaking for a few seconds. Check the box on the bottle. Be sure and close the bottle tightly after each use to avoid ethanol evaporation.

• Sample: Variety of yeast and other fungus species
• Yield: Up to 30 µg of RNA (5 x 107 Saccharomyces cerevisiae: 20 µg)
• Convenient: Includes Sorbitol Buffer to reduce sample preparation time
• Format: RNA spin columns
• Operation Time: Within 70 minutes
• Elution Volume: 50-100 µL
• Kit Storage: Dry at room temperature (15-25°C) for up to 9 months

The rYeast Total RNA Mini Kit was designed for total RNA purification from yeast and a wide variety of other fungus species. Sorbitol Buffer is included with the kit to reduce sample preparation time and minimize hands on time. Detergents and chaotropic salt are used to lyse cells and inactivate RNase while RNA is bound by the glass fiber matrix of the RNA spin column. Once any contaminants have been removed, using the Wash Buffer (containing ethanol), the purified total RNA is eluted by RNase-free Water. The entire procedure can be completed within 70 minutes and the purified RNA is ready for use in RT-PCR, Northern Blotting, Primer Extension, mRNA Selection and cDNA Synthesis.

Quality Control
The quality of the rYeast Total RNA Mini Kit is tested on a lot-to-lot basis by isolating RNA from Saccharomyces cerevisiae (5 x 10  ) harvested by centrifugation at 5,000 x g for 10 minutes. A 5 µL aliquot of purified RNA from a 50 µL eluate is analyzed by electrophoresis on a 0.8% agarose gel.

*Add absolute ethanol (see the bottle label for volume) to Wash Buffer then mix by shaking for a few seconds. Check the box on the bottle. Be sure and close the bottle tightly after each use to avoid ethanol evaporation.

IBI's Viral Nucleic Acid Extraction Kit was specifically designed for purification of viral DNA/RNA from cell-free samples; such as serum, plasma, body fluids and the supernatant of viral-infected cell cultures. These kits are recommended for parallel purification of viral DNA including HBV and CMV, as well as viral RNA including HCV, HIV and HTLV. The detection limit for certain viruses depends on the sensitivity of individual PCR or RT-PCR assays.

IBI Tri-Isolate is a phenol and guanidine isothiocyanate plus spin column system for convenient purification of high-quality total RNA from a variety of samples. Initially, samples are homogenized in IBI Isolate without chloroform phase separation or isopropanol RNA precipitation. Following sample homogenization, simply bind, wash and elute the high-quality, total RNA in RNase-free Water and use in a variety of sensitive downstream applications.

Quality Control
IBI Tri-Isolate is tested on a lot-to-lot basis. An Escherichia coli (1 x 109) culture (OD600=1.3, 1 mL) is harvested by centrifugation at 16,000 x g for 2 minutes, followed by IBI Isolate homogenization. RNA is then purified using a spin column procedure. 10 µL from a 50 µL eluate of purified RNA is analyzed by electrophoresis on a 0.8% agarose gel.

Advantages

• Purify total RNA within 15 minutes without chloroform phase separation or isopropanol RNA precipitation
• Up to: 200 µL (blood, buffy coat, serum, plasma), 5 x 106 (cultured cells), 10-50 mg (tissue), 1 x 109 (bacteria cells)
• A cost effective phenol, guanidine isothiocyanate solution plus spin column system
• High quality RNA: A260/A280 >1.8, A260/A230 >1.8
• Applications: cDNA Library Construction, Cloning, RT-PCR (Endpoint), Real-Time PCR, Nuclease Protection Assays,
• Northern Blotting

Caution
IBI Isolate contains phenol and guanidine isothiocyanate. During operation, always work in a fume hood, always wear a lab coat, disposable gloves, protective goggles and (anti-fog) procedure mask. Disposable/non-disposable glassware, plasticware and automatic pipettes should be sterile (RNase-free) and used only for RNA procedures.

Components and Storage

*Add absolute ethanol (see the bottle label for volume) to Wash Buffer prior to initial use.

IBIs MIDI and MAXI plasmid kits use pre-packed ion-exchange resin columns to purify plasmid or cosmid DNA from bacterial cultures. In the process, the modified alkaline lysis method and RNase treatment are used for creating cleared cell lysate with minimal genomic DNA and RNA contaminants. Using a gravity-flow procedure, the plasmid DNA in crude lystate has been bound to the column. he contaminants can be washed off with a wash buffer. Finally, the purified plasmid DNA is eluted by a high salt buffer and then precipitated with isopropanol for desalting. The entire procedure can be completed in less than 2 hours and the obtained high purity plasmid DNA is suitable for transfection, sequencing reactions, PCR, and in-vitro transcription.

IBI High-Speed Mini Plasmid Kit

The High-Speed Mini Plasmid Kit isolates plasmid DNA from 1-5ml cultures using alkaline lysis and RNase treatment, ensuring minimal genomic DNA/RNA contamination.

Additional Details

The High-Speed Mini Plasmid Kits by IBI Scientific are designed to process a sample size of 1-4 mL of bacterial culture, yielding 20-30 µg for high-copy plasmids and 3-10 µg for low-copy plasmids. With an operation time of 30 minutes or less and a binding capacity of up to 30 µg, these kits offer a rapid and efficient solution for plasmid DNA extraction. Check out our basic guide for plasmid kits for more information.

Technical Details

In the presence of a chaotropic salt, the plasmid DNA in the lysate binds to the glass-fiber matrix in the spin column. The contaminants are washed off with an ethanol-based wash buffer. The purified plasmid DNA is eluted by a low salt elution buffer or water. This procedure does not require DNA phenol extraction or alcohol plasmid. Typical yields are 20-30 µg for high-copy numbered plasmids or 3-10 µg for low-copy numbered plasmids. The purified plasmid DNA is ready to use for restriction enzyme digestion, ligation, PCR, or sequencing reactions. The entire procedure can be completed in under 30 minutes.

Quality Control

The quality of the High-Speed Plasmid Mini Kit is tested on a lot-to-lot basis, by isolating plasmid DNA from a 4 ml overnight E. coli (DH5α) culture, containing plasmid p Bluescript (A600>2U/mL). Following the purification process, a yield of more than 20 µg is expected and the ratio of A260/A280 is between 1.7-1.9. The purified plasmid (1 µg) is used in EcoRI digestion and checked by electrophoresis.

Physical Specifications