MIDI Flex Tube Kit

MIDI Flex Tubes combine two modes of action: electro-elution of nucleic acid molecules from polyacrylamide or agarose gels and dialysis or buffer exchange of protein molecules. Flex Tubes allow rapid, secure, simple loading and recovery, with high performance as the most convenient, user friendly, electro-elution and dialysis system on the market.

KIT CONTENTS

• Flex Tubes 2/10/30/50/ 100 pieces
• Supporting tray (for electro elution protocol) 1ea. (select kits)
• Floating rack (for dialysis protocol) 1ea. (select kits)
• Information and Protocol Manual 1ea.

SPECIFICATIONS

• Membrane cut-off: 1K(3bp), 3.5K(11bp) or 6-8K(18-24bp) MWCO
• Tube volume: 800µl
• Dialysis volume: 50-800µl
• Min. sample size for extraction: 0.5µg
• Max. gel slice: 1cm x 0.5cm
• Membrane ultra-clean, sulfur and heavy metal free. EDTA treated
• Flex Tube MWCO are in kilo Daltons (K) for proteins and corresponding base pairs (bp) for nucleic acids as indicated in the table below:

APPLICATIONS

• Dialysis, electro-elution or buffer exchange with volumes between 50-800µl
• Preparation of protein samples for MALDI-MS
• Sample concentration
• Large-scale protein dialysis, such as antibodies and recombinant protein purification
• Removal of contaminating micro-molecules
• Tissue culture extraction purification
• Removal of salts, surfactants, solvents, and detergents
• Complex formation studies (protein-protein, protein-DNA, and protein-RNA)
• pH and buffer adjustment of sample solutions, protein extraction or cell extraction
• High throughput dialysis
• Peptide dialysis, as small as 10 amino acids
• Virus-particles purification.

MAXI Flex Tube Kit

MAXI Flex Tubes combine two modes of action: electro-elution of nucleic acid molecules from polyacrylamide or agarose gels and dialysis or buffer exchange of protein molecules. Flex Tubes allow rapid, secure, simple loading and recovery, with high performance as the most convenient, user friendly, electro-elution and dialysis system on the market.

KIT CONTENTS

• Flex Tubes 2/10/30/50/ 100 pieces
• Supporting tray (for electro elution protocol) 1ea. (select kits)
• Floating rack (for dialysis protocol) 1ea. (select kits)
• Information and Protocol Manual 1ea.

SPECIFICATIONS

• Membrane cut-off: 3.5K(11bp), 6-8K(18-24bp), 12-14K(36-42bp), 25K(76bp) or 50K(152bp) MWCO
• Tube volume: 3ml
• Dialysis volume: 0.1-3ml
• Min. sample size for extraction: 20µg
• Max. gel slice: 2cm x 1cm
• Membrane ultra-clean, sulfur and heavy metal free. EDTA treated
• Flex Tube MWCO are in kilo Daltons (K) for proteins and corresponding base pairs (bp) for nucleic acids as indicated in the table below:

The Total RNA MINI and MAXI kits (Plant) provide a simple and fast method to isolate total RNA from plant tissue and cells. Samples are ground in liquid nitrogen and filtered to remove debris. In the presence of a binding buffer and chaotropic salt, the total RNA in the lysate binds to the glass fiber matrix of the spin column. The optional DNase treatments can remove DNA residues and the contaminants can be washed with an ethanol based wash buffer. Finally, the purified total RNA is eluted by RNase-free water. This protocol does not require phenol extraction or alcohol precipitation, and the entire procedure can be completed within 60 minutes. The purified total RNA is ready for RT, RT-PCR, Real Time PCR and northern blotting.

• Sample: Gram (+) positive and Gram (-) negative bacterial cells
• Yield: Up to 60 µg of RNA - (1 x 10 Escherichia coli: 40-45 µg, 1 x 10 Bacillus subtilis: 50-55 µg)
• Convenient: Includes Lysozyme and Bacteria Lysis Buffer
• Format: RNA spin columns
• Operation Time: Within 30 minutes
• Elution Volume: 50-100 µL
• Kit Storage: Dry at room temperature (15-25°C) for up to 9 months, Lysozyme should be stored at -20°C for extended periods

The rBAC Mini RNA Bacteria Kit was designed for total RNA purification from Gram (-) negative and Gram (+) positive bacteria. The provided Lysozyme and Bacteria Lysis Buffer will efficiently lyse bacterial cell walls consisting of the peptidoglycan layer. Detergents and chaotropic salt are used to further lyse cells and inactivate RNase while RNA is bound by the glass fiber matrix of the RNA spin column. Once any contaminants have been removed, using the Wash Buffer (containing ethanol), the purified total RNA is eluted by RNase-free Water and is ready for use in a variety of subsequent reactions.

Quality Control
The quality of the rBAC Mini RNA Bacteria Kit is tested on a lot-to-lot basis by isolating RNA from Escherichia coli (1x10) culture (OD600=1.3, 1 mL) harvested by centrifugation at 16,000 x g for 1 minute. 10 µL from a 50 µL eluate of purified RNA is analyzed by electrophoresis on a 0.8% agarose gel.

*Lysozyme should be stored at -20°C for extended periods. Add Lysozyme to Bacteria Lysis Buffer immediately prior to use. Once Lysozyme is mixed with Bacteria Lysis Buffer, the solution can be stored for 2 weeks at 4°C.

**Add absolute ethanol (see the bottle label for volume) to Wash Buffer then mix by shaking for a few seconds. Check the box on the bottle. Be sure and close the bottle tightly after each use to avoid ethanol evaporation.

• Sample: Variety of yeast and other fungus species
• Yield: Up to 30 µg of RNA (5 x 107 Saccharomyces cerevisiae: 20 µg)
• Convenient: Includes Sorbitol Buffer to reduce sample preparation time
• Format: RNA spin columns
• Operation Time: Within 70 minutes
• Elution Volume: 50-100 µL
• Kit Storage: Dry at room temperature (15-25°C) for up to 9 months

The rYeast Total RNA Mini Kit was designed for total RNA purification from yeast and a wide variety of other fungus species. Sorbitol Buffer is included with the kit to reduce sample preparation time and minimize hands on time. Detergents and chaotropic salt are used to lyse cells and inactivate RNase while RNA is bound by the glass fiber matrix of the RNA spin column. Once any contaminants have been removed, using the Wash Buffer (containing ethanol), the purified total RNA is eluted by RNase-free Water. The entire procedure can be completed within 70 minutes and the purified RNA is ready for use in RT-PCR, Northern Blotting, Primer Extension, mRNA Selection and cDNA Synthesis.

Quality Control
The quality of the rYeast Total RNA Mini Kit is tested on a lot-to-lot basis by isolating RNA from Saccharomyces cerevisiae (5 x 10  ) harvested by centrifugation at 5,000 x g for 10 minutes. A 5 µL aliquot of purified RNA from a 50 µL eluate is analyzed by electrophoresis on a 0.8% agarose gel.

*Add absolute ethanol (see the bottle label for volume) to Wash Buffer then mix by shaking for a few seconds. Check the box on the bottle. Be sure and close the bottle tightly after each use to avoid ethanol evaporation.

IBI's Viral Nucleic Acid Extraction Kit was specifically designed for purification of viral DNA/RNA from cell-free samples; such as serum, plasma, body fluids and the supernatant of viral-infected cell cultures. These kits are recommended for parallel purification of viral DNA including HBV and CMV, as well as viral RNA including HCV, HIV and HTLV. The detection limit for certain viruses depends on the sensitivity of individual PCR or RT-PCR assays.

IBI Tri-Isolate is a phenol and guanidine isothiocyanate plus spin column system for convenient purification of high-quality total RNA from a variety of samples. Initially, samples are homogenized in IBI Isolate without chloroform phase separation or isopropanol RNA precipitation. Following sample homogenization, simply bind, wash and elute the high-quality, total RNA in RNase-free Water and use in a variety of sensitive downstream applications.

Quality Control
IBI Tri-Isolate is tested on a lot-to-lot basis. An Escherichia coli (1 x 109) culture (OD600=1.3, 1 mL) is harvested by centrifugation at 16,000 x g for 2 minutes, followed by IBI Isolate homogenization. RNA is then purified using a spin column procedure. 10 µL from a 50 µL eluate of purified RNA is analyzed by electrophoresis on a 0.8% agarose gel.

Advantages

• Purify total RNA within 15 minutes without chloroform phase separation or isopropanol RNA precipitation
• Up to: 200 µL (blood, buffy coat, serum, plasma), 5 x 106 (cultured cells), 10-50 mg (tissue), 1 x 109 (bacteria cells)
• A cost effective phenol, guanidine isothiocyanate solution plus spin column system
• High quality RNA: A260/A280 >1.8, A260/A230 >1.8
• Applications: cDNA Library Construction, Cloning, RT-PCR (Endpoint), Real-Time PCR, Nuclease Protection Assays,
• Northern Blotting

Caution
IBI Isolate contains phenol and guanidine isothiocyanate. During operation, always work in a fume hood, always wear a lab coat, disposable gloves, protective goggles and (anti-fog) procedure mask. Disposable/non-disposable glassware, plasticware and automatic pipettes should be sterile (RNase-free) and used only for RNA procedures.

Components and Storage

*Add absolute ethanol (see the bottle label for volume) to Wash Buffer prior to initial use.

IBIs MIDI and MAXI plasmid kits use pre-packed ion-exchange resin columns to purify plasmid or cosmid DNA from bacterial cultures. In the process, the modified alkaline lysis method and RNase treatment are used for creating cleared cell lysate with minimal genomic DNA and RNA contaminants. Using a gravity-flow procedure, the plasmid DNA in crude lystate has been bound to the column. he contaminants can be washed off with a wash buffer. Finally, the purified plasmid DNA is eluted by a high salt buffer and then precipitated with isopropanol for desalting. The entire procedure can be completed in less than 2 hours and the obtained high purity plasmid DNA is suitable for transfection, sequencing reactions, PCR, and in-vitro transcription.

Fast and Economical Plasmid Minipreps

• Ideal for purification from bacterial culture
• Up to 30ug binding capacity
• Expected yield: 20-30ug for high-copy plasmid / 3-10ug for low-copy plasmid
• Perfect for cleanup prior to sequencing and cloning

The IBI High-Speed Plasmid Mini Kits are designed for the rapid isolation of plasmid or cosmid DNA from 1-4ml of bacterial cultures. The modified alkaline lysis method and RNAse treatment are used to create cleared cell lysate with minimal genomic DNA and RNA contaminants. In the presence of chaotropic salt, the plasmid DNA in the lysate binds to the glass fiber matrix in the spin column. The purified plasmid DNA is eluted by a low salt elution buffer or water. This procedure does not require DNA phenol-extraction or alcohol precipitation.

Sample:	1-4ml bacterial culture
Format:	Spin columns
Operation:	Centrifuge/Vacuum Manifold
Binding Capacity:	up to 30µg
Expectant Yield:	20-30µg for high-copy plasmid 3-10µg for low-copy plasmid
Operational Time:	30 min.
Applications:	PCR, Fluorescent or Radioactive Sequencing, Restriction Digests, DNA Labeling, Ligations

The I-Blue MINI Plasmid Kit was designed for rapid isolation of plasmid DNA from 1-7 mL of cultured bacterial cells. I-Blue Lysis Buffer (an optional color indicator) is included with the kit in order to prevent common handling errors, ensuring efficient cell lysis and neutralization. A modified alkaline lysis method and RNase treatment are used to obtain clear cell lysate with minimal genomic DNA and RNA contaminants. Typical yields are 20-35 µg for high-copy number plasmid or 3-10 µg for low-copy number plasmid from 4 mL of cultured bacterial cells. DNA phenol extraction or alcohol precipitation is not required and the entire procedure can be completed within 15 minutes. The purified plasmid DNA is ready for use in restriction enzyme digestion, ligation, PCR, and sequencing reactions.

Advantages

• Sample: 1-7 mL of cultured bacterial cells
• Yield: Up to 50 µg of pure plasmid DNA
• Format: Plasmid spin column
• Operation Time: Within 15 minutes
• Elution Volume: 30-100 µL
• Kit Storage: Dry at room temperature (15-25°C) for up to 1 year; PD1 and RNase A mixture should be stored at 2-8°C for up to 6 months

Quality Control
The quality of the I-Blue MINI Plasmid Kit is tested on a lot-to-lot basis by isolating plasmid DNA from a 4 mL overnight E. coli (DH5α) culture containing plasmid pBluescript (A600 > 2 U/mL). Following the purification process, a yield of more than 20 µg is obtained and the A260/A280 ratio is between 1.8-2.0. The purified plasmid DNA (1 µg) is used in EcoRI digestion, and analyzed by electrophoresis.

Kit Components

*For IB47171 and IB47172 add provided RNase A to PD1 Buffer then mix by shaking for a few seconds. Check the box on the bottle. PD1 and RNase A mixture should be stored at 2-8°C for up to 6 months. For IB47170 samples, RNase A was already added to PD1.

**If precipitates have formed in PD2 Buffer, warm the buffer in a 37°C water bath, followed by gentle shaking to dissolve.

***Add absolute ethanol (see the bottle label for volume) to Wash Buffer then mix by shaking for a few seconds. Check the box on the bottle. Be sure and close the bottle tightly after each use to avoid ethanol evaporation.

The Fast Ion Plasmid Maxi Kit (Endotoxin Free) uses pre-packed anion exchange resin columns to purify plasmid DNA from 100-250 ml of bacterial cultures. Modified alkaline lysis method (1) and RNase treatment are used for obtaining clear cell lysate with minimal genomic DNA and RNA contaminants. Once the plasmid DNA has been bound to the column, the contaminants can be washed off using the wash buffer. Finally, the purified plasmid DNA is eluted by a high salt buffer and precipitated with isopropanol for desalting. The entire procedure can be completed in 120 minutes and the obtained high purity plasmid DNA is suitable for transfection, sequencing reactions, PCR and in-vitro transcription.

The 96-Well Genomic DNA Kits are designed for high-throughput purification of total DNA (including genomic, mitochondrial and viral DNA) from whole blood and a variety of animal tissues or cells. This method uses Proteinase K and a chaotropic salt to lyse cells and degrade proteins. DNA in the chaotropic salt is bound by the glass fiber matrix of each well. Once any contaminants have been removed, the purified DNA is eluted by a low salt elution buffer or water. The entire procedure can be completed in 1 hour without phenol extraction or alcohol precipitation. These kits can be used for manual filtration or with robotic handling systems, and purified DNA with approximately 20-30kb is suitable for PCR or other enzymatic reactions.