Advantages

• Sample: Tissue, rodent tails, ear punches, fresh or frozen blood, serum, plasma, buffy coat, body fluids, cultured cells, amniotic fluid, FFPE, hair, insects
• Yield: Up to 5 µg of gDNA from fresh whole blood samples
• Format: genomic DNA spin column
• Operation Time: Within 20 minutes
• Elution Volume: 30-100 µL
• Kit Storage: Dry at room temperature (15-25°C) for up to 1 year

Introduction
The gMax Mini Kit is optimized for genomic, mitochondrial and virus DNA purification from whole blood (fresh blood and frozen blood), tissue, formalin-fixed paraffin-embedded tissue (FFPE), amniotic fluid and insects in one convenient kit. This DNA extraction kit uses Proteinase K and chaotropic salt to lyse cells and degrade protein, allowing DNA to bind to the glass fiber matrix of the spin column. Contaminants are removed using a Wash Buffer and the purified genomic DNA is eluted by a low salt Elution Buffer, TE or water. The entire procedure can be completed within 20 minutes without phenol/chloroform extraction or alcohol precipitation. The purified DNA (approximately 20-30 kb) is suitable for use in PCR or other enzymatic reactions.

Quality Control
The quality of the gMax Mini Kit is tested on a lot-to-lot basis by isolating genomic DNA from 200 μL of whole human blood. The purified DNA (5 μg with an A260/A280 ratio of 1.8-2.0) is quantified with a spectrophotometer and analyzed by electrophoresis.

Kit Components

*Add absolute ethanol (see the bottle label for volume) to Wash Buffer then mix by shaking for a few seconds. Check the box on the bottle. Be sure and close the bottle tightly after each use to avoid ethanol evaporation.

**Add ddH2O to Proteinase K (see the bottle label for volume) then vortex to ensure Proteinase K is completely dissolved. Check the box on the bottle. Once it is dissolved completely, centrifuge for a few seconds to spin the mixture down. For extended periods, the ddH2O and Proteinase K mixture should be stored at 4°C.

IBI MINI miRNA Isolation

IBI MINI miRNA Isolation

The miRNA Isolation Kits are designed for purification of micro RNA (miRNA) and other small cellular RNAs from tissue samples or cultured cells. Purification of miRNA allows research into biological significant pathways for gene regulation. The standard protocol for isolating total RNA and mRNA are not optimized for isolation of small RNA molecules and result in the loss of substantial amounts of miRNA and other small RNA. 

REPLACEMENT GD COLUMNS & COLLECTION TUBES - 100 PACK

• For use with leftover reagents from IBI Genomic DNA Kits (Tissue, Blood & Cultured Cells) or competitive products
• Sample Size: 300 mL fresh whole blood/107 animal cultured cells
• Elution Volume: 50 to 200 µL
• Binding Capacity: Up to 10 µg

The Replacement Genomic DNA Columns & Collection Tubes are for use with leftover reagents from IBI Genomic DNA Kits including the following item numbers: IB47201, IB47202, IB47211, IB47221, IB47222, IB47231, IB47241, IB47242, IB47281, IB47282, IB47291, and IB47292.

The columns and collection tubes can also be used with comparable, competitive Genomic DNA Kit reagents that utilize a lysis, bind, wash, elute method. However, you must observe the sample size, binding capacity, and elution volume for the replacement column stated in the specifications.

IBI MIDI I-Blue Plasmid Kit

The I-Blue MINI Plasmid Kit was designed for rapid isolation of plasmid DNA from 1-7 mL of cultured bacterial cells. I-Blue Lysis Buffer (an optional color indicator) is included with the kit in order to prevent common handling errors, ensuring efficient cell lysis and neutralization. A modified alkaline lysis method and RNase treatment are used to obtain clear cell lysate with minimal genomic DNA and RNA contaminants. Typical yields are 20-35 µg for high-copy number plasmid or 3-10 µg for low-copy number plasmid from 4 mL of cultured bacterial cells. DNA phenol extraction or alcohol precipitation is not required and the entire procedure can be completed within 15 minutes. The purified plasmid DNA is ready for use in restriction enzyme digestion, ligation, PCR, and sequencing reactions.

Advantages

• Sample: 1-7 mL of cultured bacterial cells
• Yield: Up to 50 µg of pure plasmid DNA
• Format: Plasmid spin column
• Operation Time: Within 15 minutes
• Elution Volume: 30-100 µL
• Kit Storage: Dry at room temperature (15-25°C) for up to 1 year; PD1 and RNase A mixture should be stored at 2-8°C for up to 6 months

Quality Control

PR1MA™ MS2 Phage

PR1MA™ MS2 is an E. coli bacteriophage with a single-stranded RNA genome of 3569 nucleotides protected from nuclease degradation by a capsid of 180 coat protein monomers. This virus is a Biosafety Level 1 organism, not pathogenic to humans. These properties make MS2 phage useful as a process control in nucleic acid-based amplification techniques like RT-PCR, RT-LAMP, particularly those that involve viral RNA extraction.

• Biosafety level 1
• Lysis: 65°C for 20 minutes or by standard RNA extraction
• Storage temperature: 4°C 

Buffer composition

• 10 mM Tris-HCl
• 0.1  mM EDTA
• pH = 8.0 

*These products are intended for research use only, not for therapeutic or diagnostic purposes in humans or animals. The safety and efficacy of these products in diagnostic or other clinical uses has not been established.
 

IBI Small DNA Fragment

IBI Small DNA Fragment

REPLACEMENT PD COLUMNS AND COLLECTION TUBES -100 PACK

• For use with leftover reagents from IBI Plasmid Kits or competitive products
• Sample Size: 1 -4 mL bacterial culture
• Elution Volume: 50 to 100 µL
• Binding Capacity: Up to 30 µg

The Replacement Mini Plasmid Columns and Collection Tubes are for use with leftover reagents from IBI Mini Plasmid Kits including the following item numbers: IB47101, IB47102, IB47170, IB47171, and IB47172.

The columns and collection tubes can also be used with comparable, competitive Mini Plasmid Kit reagents that utilize a bind, wash, and elute method. However, you must observe the sample size, binding capacity, and elution volume for the replacement column stated in the specifications.

SPECIFICATIONS

Sample Size: 1 - 4 mL bacterial culture
Format: Spin column or vacuum manifold
Expected yield: 20 -30 µg for high copy and 3 -10 µg for low copy.
Elution volume: 50 µL to 100 µL
Operation time: 15 minutes
Purified plasmid DNA is ready for use in ligation, PCR, restriction enzyme digestion, and sequencing reactions.

PR1MA™ RNase Inhibitor

PR1MA™ RNase Inhibitor (RI) is a 50 kDa protein that specifically inhibits RNases A, B, and C by binding noncovalently in a 1:1 ratio at high affinity. It can be used in isothermal amplification and molecular diagnostic assays, cDNA synthesis, and other applications where RNA stability is important. It is ineffective against RNase1, T1, S1 Nuclease, or RNase H. It has no inhibition of polymerase activity when used with Taq DNA polymerase, AMV, M-MuLV, HIV reverse transcriptases, or phage RNA polymerases.

Storage Buffer

• 50% glycerol
• 10 mM Tris-HCl
• 50 mM KCl
• 8 mM DTT
• pH = 7.5 

*To prevent the release of ribonuclease from RNase Inhibitor, temperatures greater than 50°C and high concentrations of denaturing reagents such as urea should be avoided.

*These products are intended for research use only, not for therapeutic or diagnostic purposes in humans or animals. The safety and efficacy of these products in diagnostic or other clinical uses has not been established.