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Advantages
- Sample: Tissue, rodent tails, ear punches, fresh or frozen blood, serum, plasma, buffy coat, body fluids, cultured cells, amniotic fluid, FFPE, hair, insects
- Yield: Up to 5 µg of gDNA from fresh whole blood samples
- Format: genomic DNA spin column
- Operation Time: Within 20 minutes
- Elution Volume: 30-100 µL
- Kit Storage: Dry at room temperature (15-25°C) for up to 1 year
Introduction
The gMax Mini Kit is optimized for genomic, mitochondrial and virus DNA purification from whole blood (fresh blood and frozen blood), tissue, formalin-fixed paraffin-embedded tissue (FFPE), amniotic fluid and insects in one convenient kit. This DNA extraction kit uses Proteinase K and chaotropic salt to lyse cells and degrade protein, allowing DNA to bind to the glass fiber matrix of the spin column. Contaminants are removed using a Wash Buffer and the purified genomic DNA is eluted by a low salt Elution Buffer, TE or water. The entire procedure can be completed within 20 minutes without phenol/chloroform extraction or alcohol precipitation. The purified DNA (approximately 20-30 kb) is suitable for use in PCR or other enzymatic reactions.
Quality Control
The quality of the gMax Mini Kit is tested on a lot-to-lot basis by isolating genomic DNA from 200 μL of whole human blood. The purified DNA (5 μg with an A260/A280 ratio of 1.8-2.0) is quantified with a spectrophotometer and analyzed by electrophoresis.
Kit Components
Component |
IB47280 |
IB47281 |
IB47282 |
GST Buffer |
3 mL |
30 mL |
75 mL |
GSB Buffer |
4 mL |
40 mL |
75 mL |
W1 Buffer |
2 mL |
45 mL |
130 mL |
Wash Buffer* |
1 mL |
25 mL |
50 mL |
Proteinase K** |
1 mg |
11 mg x 2 |
65 mg |
Elution Buffer |
1 mL |
30 mL |
75 mL |
GD Columns |
4 |
100 |
300 |
2 mL Collection Tubes |
8 |
200 |
600 |
*Add absolute ethanol (see the bottle label for volume) to Wash Buffer then mix by shaking for a few seconds. Check the box on the bottle. Be sure and close the bottle tightly after each use to avoid ethanol evaporation.
**Add ddH2O to Proteinase K (see the bottle label for volume) then vortex to ensure Proteinase K is completely dissolved. Check the box on the bottle. Once it is dissolved completely, centrifuge for a few seconds to spin the mixture down. For extended periods, the ddH2O and Proteinase K mixture should be stored at 4°C.
IBI MINI miRNA Isolation
The miRNA Isolation Kits are designed for purification of micro RNA (miRNA) and other small cellular RNAs from tissue samples or cultured cells. Purification of miRNA allows research into biological significant pathways for gene regulation. The standard protocol for isolating total RNA and mRNA are not optimized for isolation of small RNA molecules and result in the loss of substantial amounts of miRNA and other small RNA.
In addition, removal of the predominantly larger RNAs is required for accurate analysis of miRNA expression by qPCR or microarray analysis. This kit is specifically designed for purification of small RNA with minimal contamination from large RNA molecules or genomic DNA. The method employs a spin column with a silica-based fiber matrix that binds RNA in the presence of a chaotropic salt. The method is based on the selective binding of RNA molecules of various sizes to the silica-based fiber matrix when different ethanol concentrations are present in the solvent.
IBI MINI miRNA Isolation
The miRNA Isolation Kits are designed for purification of micro RNA (miRNA) and other small cellular RNAs from tissue samples or cultured cells. Purification of miRNA allows research into biological significant pathways for gene regulation. The standard protocol for isolating total RNA and mRNA are not optimized for isolation of small RNA molecules and result in the loss of substantial amounts of miRNA and other small RNA.
REPLACEMENT GD COLUMNS & COLLECTION TUBES - 100 PACK
- For use with leftover reagents from IBI Genomic DNA Kits (Tissue, Blood & Cultured Cells) or competitive products
- Sample Size: 300 mL fresh whole blood/107 animal cultured cells
- Elution Volume: 50 to 200 µL
- Binding Capacity: Up to 10 µg
The Replacement Genomic DNA Columns & Collection Tubes are for use with leftover reagents from IBI Genomic DNA Kits including the following item numbers: IB47201, IB47202, IB47211, IB47221, IB47222, IB47231, IB47241, IB47242, IB47281, IB47282, IB47291, and IB47292.
The columns and collection tubes can also be used with comparable, competitive Genomic DNA Kit reagents that utilize a lysis, bind, wash, elute method. However, you must observe the sample size, binding capacity, and elution volume for the replacement column stated in the specifications.
IBI MIDI I-Blue Plasmid Kit
The I-Blue MINI Plasmid Kit was designed for rapid isolation of plasmid DNA from 1-7 mL of cultured bacterial cells. I-Blue Lysis Buffer (an optional color indicator) is included with the kit in order to prevent common handling errors, ensuring efficient cell lysis and neutralization. A modified alkaline lysis method and RNase treatment are used to obtain clear cell lysate with minimal genomic DNA and RNA contaminants. Typical yields are 20-35 µg for high-copy number plasmid or 3-10 µg for low-copy number plasmid from 4 mL of cultured bacterial cells. DNA phenol extraction or alcohol precipitation is not required and the entire procedure can be completed within 15 minutes. The purified plasmid DNA is ready for use in restriction enzyme digestion, ligation, PCR, and sequencing reactions.
Advantages
- Sample: 1-7 mL of cultured bacterial cells
- Yield: Up to 50 µg of pure plasmid DNA
- Format: Plasmid spin column
- Operation Time: Within 15 minutes
- Elution Volume: 30-100 µL
- Kit Storage: Dry at room temperature (15-25°C) for up to 1 year; PD1 and RNase A mixture should be stored at 2-8°C for up to 6 months
Quality Control
Isolation of micro RNA (miRNA) from Tissue or Cultured Cells
The miRNA Isolation Kits are designed for purification of micro RNA (miRNA) and other small cellular RNAs from tissue samples or cultured cells. Purification of miRNA allows research into biological significant pathways for gene regulation. The standard protocol for isolating total RNA and mRNA are not optimized for isolation of small RNA molecules and result in the loss of substantial amounts of miRNA and other small RNA.
In addition, removal of the predominantly larger RNAs is required for accurate analysis of miRNA expression by qPCR or microarray analysis. This kit is specifically designed for purification of small RNA with minimal contamination from large RNA molecules or genomic DNA. The method employs a spin column with a silica-based fiber matrix that binds RNA in the presence of a chaotropic salt. The method is based on the selective binding of RNA molecules of various sizes to the silica-based fiber matrix when different ethanol concentrations are present in the solvent.
Sample Types: |
100mg tissue or 1 x 10 cultured cells |
Format: |
Spin column |
Operation Time: |
30 minutes |
IBI Small DNA Fragment
The Gel/PCR DNA Fragments Extraction Kits are designed to recover or concentrate DNA fragments (50bp-10kb) from agarose gel, PCR reaction, or any other enzymatic reaction. This method uses a chaotropic salt and guanidine thiocyanate to dissolve the agarose gel and denature the enzymes. The DNA fragments in the chaotropic salt are bound to the glass-fiber matrix of the spin column. After washing off the contaminants, the purified DNA fragments are eluted by low salt elution buffer or water. Salts, enzymes, and unincorporated nucleotides can be effectively removed from the reaction mixture without phenol extraction or alcohol precipitation.
IBI Small DNA Fragment
The Gel/PCR DNA Fragments Extraction Kits are designed to recover or concentrate DNA fragments (50bp-10kb) from agarose gel, PCR reaction, or any other enzymatic reaction. This method uses a chaotropic salt and guanidine thiocyanate to dissolve the agarose gel and denature the enzymes. The DNA fragments in the chaotropic salt are bound to the glass-fiber matrix of the spin column. After washing off the contaminants, the purified DNA fragments are eluted by low salt elution buffer or water. Salts, enzymes, and unincorporated nucleotides can be effectively removed from the reaction mixture without phenol extraction or alcohol precipitation.
Isolation of micro RNA (miRNA) from Tissue or Cultured Cells
The miRNA Isolation Kits are designed for purification of micro RNA (miRNA) and other small cellular RNAs from tissue samples or cultured cells. Purification of miRNA allows research into biological significant pathways for gene regulation. The standard protocol for isolating total RNA and mRNA are not optimized for isolation of small RNA molecules and result in the loss of substantial amounts of miRNA and other small RNA.
In addition, removal of the predominantly larger RNAs is required for accurate analysis of miRNA expression by qPCR or microarray analysis. This kit is specifically designed for purification of small RNA with minimal contamination from large RNA molecules or genomic DNA. The method employs a spin column with a silica-based fiber matrix that binds RNA in the presence of a chaotropic salt. The method is based on the selective binding of RNA molecules of various sizes to the silica-based fiber matrix when different ethanol concentrations are present in the solvent.
Sample Types: |
100mg tissue or 1 x 10 cultured cells |
Format: |
Spin column |
Operation Time: |
30 minutes |
REPLACEMENT PD COLUMNS AND COLLECTION TUBES -100 PACK
- For use with leftover reagents from IBI Plasmid Kits or competitive products
- Sample Size: 1 -4 mL bacterial culture
- Elution Volume: 50 to 100 µL
- Binding Capacity: Up to 30 µg
The Replacement Mini Plasmid Columns and Collection Tubes are for use with leftover reagents from IBI Mini Plasmid Kits including the following item numbers: IB47101, IB47102, IB47170, IB47171, and IB47172.
The columns and collection tubes can also be used with comparable, competitive Mini Plasmid Kit reagents that utilize a bind, wash, and elute method. However, you must observe the sample size, binding capacity, and elution volume for the replacement column stated in the specifications.
SPECIFICATIONS
Sample Size: 1 - 4 mL bacterial culture
Format: Spin column or vacuum manifold
Expected yield: 20 -30 µg for high copy and 3 -10 µg for low copy.
Elution volume: 50 µL to 100 µL
Operation time: 15 minutes
Purified plasmid DNA is ready for use in ligation, PCR, restriction enzyme digestion, and sequencing reactions.
REPLACEMENT DF COLUMNS AND COLLECTION TUBES - 100 PACK
- For use with leftover reagents from IBI PCR/Gel Extraction Kits or competitive products
- Sample Size: 300 mg agarose gel/100 µL PCR product
- Elution Volume: 20 to 50 µL
- Binding Capacity: Up to 10 µg
The Replacement DF Columns and Collection Tubes are for use with leftover reagents from IBI PCR/Gel Extraction Kits including the following item numbers: IB47020 and IB47030.
The replacement spin columns and collection tubes can also be used with comparable, competitive PCR/Gel Extraction Kit reagents that utilize a bind, wash, elute method. However, you must observe the sample size, binding capacity, and elution volume for the replacement column stated in the specifications.
SPECIFICATIONS
Sample Size: 300 mg of agarose gel or 100 µL of PCR product
Format: Spin column or vacuum manifold
Expected yield: 90% for Gel extraction and 95% for PCR Clean-up
Elution volume: 20 µL to 50 µL
Operation time: 20 minutes
Purified DNA is ready for use in Fluorescent or Radioactive sequencing, ligation, PCR, restriction enzyme digestion, and DNA labeling.
IBI 96-Well Vacuum Manifold
- Optimized for use with all of IBI 96-well kits
- The thin upper portion of the manifold is designed to reduce cross contamination
- Allows for the most effective extraction and purification of plasmid DNA, genomic DNA, viral DNA & RNA, total RNA and PCR products
- Materials: Manifold - made of anodized aluminum, Gasket - Ethylene propylene, O-Ring - Silicone
- Dimensions: 17x12x8cm
- Maximum vacuum: Approx. 71cm Hg (28 in Hg) (-13.7psi)
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