Molecular Biology Reagents/Chemicals

MIDSCI™ gives you ultra-pure, cost-competitive molecular biology reagents and lab chemicals for life science research; often with significant cost savings over Sigma Aldrich and other lab reagent providers.

PR1MA™ Chemicals can help with a wide variety of research-based requirements.
 
Use our glycerol for polyacrylamide gel electrophoresis. Eliminate Agrobacterium after inoculation with the carbenicillin we carry. Or detect β-Galactosidase activity with X-GAL which, when combined with the IPTG we stock, can be used to detect lac gene expression in cloning applications.
 
We also carry Luria Broth (Lennox), for the propagation and maintenance of E. coli; DTT, in order to maintain –SH groups in a reduced state; and high gel strength agar for tissue culture and microbiological work.

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PR1MA  Colistin Sulfate

CAS Number: 1264-72-8
Molecular Weight: 2801.27
Chemical Formula: (C52H98N16O13 )2  5(H2SO4)
Solubility: Water
Storage Temperature: Short Term: 2-8°C; Long Term: -20°C


Active against gram negative bacteria. Potency approximately 20,000 units per milligram.


Research or further manufacturing use only, not for food or drug use.


  • Appearance: White or almost white powder
  • Arsenic (As):  2 ppm
  • Heavy Metals : ≤ 20 ppm
  • Identification: Conforms
  • Loss on Drying:  3.5 %
  • pH (1% in H2O): 4.0 - 6.0
  • Potency (Dried basis):  19000 µg/mg
  • Solubility (50mg/ml in H2O): Clear and light yellow solution
  • Specific Rotation: -73 - -63 °
  • Sulfate: 16.0 - 18.0 %
  • Sulfated Ash:  1.0 %

PR1MA  Polymixin B Sulfate

CAS Number: 1405-20-5
Molecular Weight: 1385.61
Chemical Formula: C55H96N16O13  2H2SO4
Solubility: Water
Storage Temperature: 2-8°C


Mixture of sulfates of polypeptides from certain strains of Bacillus polymixa. Active against gram negative bacteria.


Research or further manufacturing use only, not for food or drug use.


  • Appearance: White to Light Yellow Powder
  • Identification: Pass Test
  • pH (0.5% Solution): 5.0 - 7.5
  • Loss on Drying:  7.0 %
  • Single Impurity:  3.0 %
  • Total impurities:  17.0 %
  • Bacteria Colonies:  10/g
  • Ecoli Colonies: Should not be found
  • Fungi and Yeast colonies:  10/g
  • Assay, HPLC (on dried basis) (Sum of polymxin B1,B2, B3 and B1-1):  80.0 %
  • Assay, HPLC (on dried basis) (Polymyxin B3):  6.0 %
  • Assay, HPLC (on dried basis) (Polymyxin B1-1):  15.0 %
  • Assay (Dried Basis):  6000 U/mg
  • Assay (Wet Basis): Report U/mg
  • Non Animal Origin: Non Animal Origin
  • Grade: USP
  • Country of Origin: Report

PR1MA Agar Noble

CAS Number: 9002-18-0
Solubility: Water with Heat
Storage Temperature: Room Temperature


Ultra-high purity Agar recommended for use in applications where maximum clarity and purity are essential. Thorough bleaching and washing procedures are utilized to remove trace amounts of ash and mineral contaminants.


Concentrations of 3-8 g/L obtains a semi-solid gel.


Research or further manufacturing use only, not for food or drug use.


  • Appearance: White to pale yellow crystalline powder
  • ASH (Residue Ignition):  1.6 %
  • Gelling Point: 32 - 39 °C
  • Melting Point: 80 - 95 oC
  • Moisture :  12.0 %
  • pH (1.5% solution): 5.5 - 7.2

PR1MA Acrylamide/bis-Acrylamide, 37.5:1 Ratio Solution

CAS Number: 79-06-1; 110-26-9; 7732-18-5
Storage Temperature: 2-8°C


A 40% solution containing 38.96% (w/v) Acrylamide and 1.04% (w/v) bis-Acrylamide for a monomer to crosslinker ratio of 37.5:1.


WARNING!
This product can expose you to Acrylamide, a chemical which is known to the State of California to cause cancer, developmental and reproductive harm. For more information go to www.P65Warnings.ca.gov.


Research or further manufacturing use only, not for food or drug use.


  • Appearance: Clear, colorless solution
  • Conductivity of Solution:  10 µmhos
  • Electrophoresis: Pass Test
  • pH: Report

PR1MA  Spectinomycin Dihydrochloride Pentahydrate

 

CAS Number: 22189-32-8
Molecular Weight: 495.3
Chemical Formula: C14H24N2O7  2HCl  5H2O
Solubility: Water
Storage Temperature: 2-8°C


Aminocyclitol antibiotic that inhibits protein synthesis. Cell culture tested.


Research or further manufacturing use only, not for food or drug use.


  • Appearance: White or almost white, Slightly hygroscopic powder
  • Identification: Pass Test
  • pH @25 °C: 3.8 - 5.6
  • Potency :  603 IU/mg
  • Residue after Ignition:  1.0 %
  • Solubility: Clear and colorless to light yellow
  • Specific Rotation: +15 - +21 °
  • Water (KF): 16.0 - 20.0 %

PR1MA  Luria Agar Granulated [Miller's LB Agar]

Common Name: Miller's LB Agar
CAS Number: 91079-40-2; 8013-01-2; 7647-14-5; 9002-18-0
Solubility: Water with Heat
Storage Temperature: Room Temperature


For propagation and maintenance of E. Coli.


Dissolve 37 grams in 1 Liter distilled water and adjust the pH to 7.2. Sterilize by autoclaving for 15 minutes.


Ingredients:

  • Tryptone: 10g/l
  • Sodium Chloride: 10g/l
  • Yeast Extract: 5g/l
  • Agar: 12g/l

Research or further manufacturing use only, not for food or drug use.


  • DCM Appearance: Homogeneous Free Flowing,Light Beige
  • PPM Appearance: Clear, none to light ppt, light yellow
  • pH (2.5 % Solution @25 oC): 6.8 - 7.2
  • Microorganism (E. coli ATCC 23724): Good to Excellent
  • Microorganism (E. coli ATCC 33694): Good to Excellent
  • Microorganism (E. coli ATCC 33849): Fair to Excellent
  • Microorganism (E. coli ATCC 39403): Good to Excellent
  • Microorganism (E. coli ATCC 47014): Good to Excellent
  • Microorganism (E. coli ATCC 53868): Good to Excellent

PR1MA Mitomycin C

CAS Number: 50-07-7
Molecular Weight: 334.3
Chemical Formula: C15H18N4O5
Solubility: Ethanol
Storage Temperature: 2-8°C

 
Antineoplastic antibiotic that acts as an alkylating agent after activation and also suppresses the synthesis of nucleic acids. A cell-cylce non specific agent, most active in the late G1 and early S phases.

WARNING! This product can expose you to Mitomycin-C, a chemical which is known to the State of California to cause cancer. For more information go to www.P65Warnings.ca.gov.

Research or further manufacturing use only, not for food or drug use.
 

  • Appearance: Blue violet solid
  • Identification (UV): Conforms
  • Purity-HPLC (RP CH3CN:H2O-0-100%):  99.0 %
  • Purity-HPLC (RP MEOH:H2O-90-10):  98.0 %
  • Purity-TLC (CH2CL2:MEOH- 9:1):  98.0 %
  • Solubility (10 mg/ml MEOH): Clear Blue Solution


 

PR1MA DTT [DL-Dithiothreitol] (Cleland's Reagent)]

Common Name: [DL-Dithiothreitol] (Cleland's Reagent)]
CAS Number: 3483-12-3
Molecular Weight: 154.25
Chemical Formula: C4H10O2S2
Solubility: Water
Storage Temperature: 2-8°C


Protective reagent for maintaining -SH groups in reduced state. DTT permeates the cell membrane protecting protein sulfhydryls thereby restoring enzyme activity lost by oxidation of the groups.


Research or further manufacturing use only, not for food or drug use.


  • Appearance: A white free flowing powder, hygroscopic
  • Solubility: Freely soluble in methanol, methylene chloride
  • Clarity in water: 5% (w/v) solution in water is clear and colorless
  • Identification by TLC: Rf value of the sample is comparable with that of the standard
  • Melting Range: 37.0 - 44.0 °C
  • Related Substance (UV Spectroscopy- Content of Oxidised DTT):  0.4 %
  • Assay (By Iodimetry):  98.0 %
  • Country of Origin : Report

[N-(2-Hydroxyethyl) piperazine N'-(2-ethanesulfonic acid)]

PR1MA  HEPES, Free Acid [N-(2-Hydroxyethyl) piperazine N'-(2-ethanesulfonic acid)]

Common Name: [N-(2-Hydroxyethyl) piperazine N'-(2-ethanesulfonic acid)]
CAS Number: 7365-45-9
Molecular Weight: 238.3
Chemical Formula: C8H18N2O4S
Solubility: Water
Storage Temperature: Room Temperature



Zwitterionic N-substituted aminosulfonic acid buffer.

  • pka (25°C): 7.5
  • pH Range (Working): 6.8 - 8.2
  • Heavy Metals: <0.001% Max

Research or further manufacturing use only, not for food or drug use.


  • Appearance: White Crystalline Powder
  • Assay (Titration, dried basis):  99.5 %
  • DNase, RNase, protease: Not Detected
  • Endotoxin (0.2% Solution):  0.25 EU/ml
  • Heavy Metals (as Pb):  0.001 %
  • Identification (IR): Pass
  • Iron (Fe):  0.0005 %
  • Loss on Drying:  1.0 %
  • pH (5% in water): 5.0 - 6.5
  • pKa @ 20°C: 7.35 - 7.70
  • Residue on Ignition:  0.1 %
  • Solubility (1M Solution in Water ) : Clear and Colorless Solution
  • Sulfate:  0.05 %
  • UV Absorbance (1M@260nm):  0.05
  • UV Absorbance (1M@280nm):  0.05

Premixed Powder 19:1 Ratio

PR1MA™ Acrylamide/bis-Acrylamide, Premixed Powder 19:1 Ratio

Storage Temperature: Room Temperature


Pre-measured powder blend of acrylamide and bis-acrylamide for simple stock solution preparation.


WARNING!
This product can expose you to Acrylamide, a chemical which is known to the State of California to cause cancer, developmental and reproductive harm. For more information go to www.P65Warnings.ca.gov.


Research or further manufacturing use only, not for food or drug use.


  • Appearance: Powder
  • Solubility: Clear and complete
  • Conductivity:  10 µmhos

PR1MA Puromycin Dihydrochloride

CAS Number: 58-58-2
Molecular Weight: 544.43
Chemical Formula: C22H29N7O5 
 2HCl
Solubility: Water
Storage Temperature: -20°C


Amino nucleoside antibiotic allows the selection of cells expressing the pac gene. Inhibits the growth of gram-positive bacteria and various animal and insect cells.

Research or further manufacturing use only, not for food or drug use.
 

  • Appearance: White to Off White Powder
  • Identification (TLC): Conforms
  • Melting Point: 168 - 182 oC
  • Purity (HPLC):  98.0 %
  • Solubility: Clear, colorless or light yellow solution
  • Water (KF):  12.0 %


 

PR1MA  Peptone, Soy

 
CAS Number: 91079-46-8
Solubility: Water
Storage Temperature: Room Temperature


From papaic hydrolysis of soybean meal. Low NaCl content.
 

Research or further manufacturing use only, not for food or drug use.
 

  • Amino Nitrogen:  2.2 %
  • Ash:  15 %
  • Loss on Drying:  6.0 %
  • pH (2% water) @25°C: 6.5 - 7.5
  • Total Nitrogen:  7.0 %
  • Coliform: Negative
  • E.Coli: Negative
  • Molds and Yeasts:  100 cfu/g
  • Salmonella: Negative /25g
  • Standard Plate Count :  5000 cfu/g
  • Broth Formulations (Growth Supporting Properties): Satisfactory
  • Peptone Agar (Growth Supporting Properties): Satisfactory
  • Country of Origin of Raw Material : Report
  • Raw Material Species: Plant
  • Country of Origin of Enzymes : Report
  • Enzyme Species: Porcine
  • Country Of Manufacture: Report
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