Molecular Biology Reagents/Chemicals

MIDSCI™ gives you ultra-pure, cost-competitive molecular biology reagents and lab chemicals for life science research; often with significant cost savings over Sigma Aldrich and other lab reagent providers.

PR1MA™ Chemicals can help with a wide variety of research-based requirements.
 
Use our glycerol for polyacrylamide gel electrophoresis. Eliminate Agrobacterium after inoculation with the carbenicillin we carry. Or detect β-Galactosidase activity with X-GAL which, when combined with the IPTG we stock, can be used to detect lac gene expression in cloning applications.
 
We also carry Luria Broth (Lennox), for the propagation and maintenance of E. coli; DTT, in order to maintain –SH groups in a reduced state; and high gel strength agar for tissue culture and microbiological work.

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Pall® Ampoule Media for Microbiological Analysis

  • Sterile Ampoule Media Pre-Measured for Maximum Efficiency and Convenience
  • Meets Membrane Filter (MF) Technique Standard Method requirements with 2 mL ampoules*
  • Maximizes efficiency with premixed and presterilized media
  • Choose between plastic or glass ampoules for your applications
  • Simplify pouring of the media with wide-mouth glass ampoules
  • All plastic ampoule media is economically packaged with 50 ampoules per box

Item#:
ASAMPOULEMEDIA

PR1MA LB Broth Powder [Lennox L Broth]

Common Name: Lennox L Broth
Solubility: Water
Storage Temperature: Room Temperature


For propagation and maintenance of E. Coli. Low Salt Formula. Molecular Biology Grade.


Dissolve 20 grams in 1 liter distilled water. Then adjust pH to 7.2. Heat and frequently agitate to completely dissolve. Sterilize by autoclaving for 15 minutes.


Ingredients:

  • Tryptone: 10 g/L
  • Yeast Extract: 5 g/L
  • Sodium Chloride: 5 g/L



Research or further manufacturing use only, not for food or drug use.


  • DCM Appearance: Homogeneous free flowing, light beige
  • PPM Appearance: Clear, No to light ppt, light yellow
  • pH (2% water) @25°C: 6.8 - 7.2
  • Microorganism (E. coli ATCC 23724): Good to Excellent
  • Microorganism (E. coli ATCC 33694): Good to Excellent
  • Microorganism (E. coli ATCC 33849): Fair to Excellent
  • Microorganism (E. coli ATCC 39403): Good to Excellent
  • Microorganism (E. coli ATCC 47014): Good to Excellent
  • Microorganism (E. coli ATCC 53868): Good to Excellent
  • Sodium Chloride: 5 g/L
  • Yeast Extract: 5 g/L
  • Casein Peptone: 10 g/L

PR1MA  Blasticidin S Hydrochloride Powder

CAS Number: 3513-03-9
Molecular Weight: 458.9
Chemical Formula: C17H26N8O5  HCl
Solubility: Water
Storage Temperature: -20°C


A peptidyl nucleoside antibiotic isolated from the culture broth of Streptomyces griseogenes, inhibits protein synthesis in both prokaryotes and eukaryotes.

 
Research or further manufacturing use only, not for food or drug use.
 
  • Appearance: White Powder
  • Endotoxin :  1.0 EU/mg
  • Identification: Pass Test
  • Potency:  850 µg/mg
  • Purity (HPLC):  98.0 %
  • Solubility: Clear, Slightly yellow solution at 5 mg/mL in water

PR1MA  Tris Base Ultra Pure [Tris (Hydroxymethyl) Aminomethane]

Common Name: Tris (Hydroxymethyl) Aminomethane
CAS Number: 77-86-1
Molecular Weight: 121.1
Chemical Formula: C4H11NO3
Solubility: Water
Storage Temperature: Room Temperature


Highly purified for use in molecular biology applications.


Moisture: <0.2%
Color: <20 APHA (colorless)
Heavy Metals (as Pb): <0.0001%
Insoluble Matter: <0.005%
Heavy Metals: 5 ppm
pKa (20°C): 8.1 ± 0.1


Research or further manufacturing use only, not for food or drug use.
 

  • Appearance: White Crystalline Powder
  • Purity (Dried Basis By GC):  99.9 %
  • Related Substances By GC:  0.1 %
  • Water:  0.2 %
  • Loss on Drying @ 105 oC, 3hrs:  1.0 %
  • Melting Point: 170 - 172 oC
  • APHA Color, 20% Aqueous solution:  20
  • Insoluble Matter:  0.005 %
  • Heavy Metals (as Pb):  5 ppm
  • Residue on Ignition:  0.5 %
  • Iron (Fe):  5 ppm
  • UV Absorbance @260nm, 10% Aqueous solution :  0.03
  • UV Absorbance @280nm, 10% Aqueous solution :  0.02
  • UV Absorbance @290nm, 40% solution in H2O:  0.20
  • UV Absorbance @430nm, 10% Aqueous solution :  0.004
  • Identification A: Pass
  • Identification B: Pass
  • Identification C: Pass
  • pH (5% in water): 10.0 - 11.5
  • Arsenic (As):  1 ppm
  • Chloride:  100 ppm
  • Copper (Cu):  1 ppm
  • DNase, RNase, protease: None Detected
  • Lead:  1 ppm
  • Magnesium (Mg):  1 ppm
  • pH (1M in H2O): 10.5 - 11.5
  • Residual Solvents: Conforms to USP/EP
  • Residue on Ignition (USP):  0.1 %
  • Solubility (2M In H2O): Pass

PR1MA Terrific Broth, Modified, Granulated

CAS Number: 91079-40-2; 8013-01-2; 7778-77-0; 7758-11-4
Solubility: Water
Storage Temperature: Room Temperature


Enriched media for maintenance and propagation of E. Coli and plasmid growth. Produces higher cell densities resulting in greater yields of DNA or recombinant problem. Glycerol is used as a carbon source with this formulation instead of glucose as it will not ferment to acetic acid.


Ingredients:

  • Casein Digest Peptone: 12g/L
  • Yeast Extract: 24g/L
  • Dipotassium phosphate: 9.5g/L
  • Monopotassium phosphate: 2.2g/L



Research or further manufacturing use only, not for food or drug use.


  • DCM Appearance: Homogeneous, free flowing beige to yellow-beige
  • PPM Appearance: Clear, yellow to amber no precipitate (ppt)
  • pH (4.76% solution at 25°C): 6.8 - 7.4
  • Microorganism (E. coli ATCC 23724): Good to Excellent
  • Microorganism (E. coli ATCC 33694): Good to Excellent
  • Microorganism (E. coli ATCC 33849): Good to Excellent
  • Microorganism (E. coli ATCC 39403): Good to Excellent
  • Microorganism (E. coli ATCC 47014): Good to Excellent
  • Microorganism (E. coli ATCC 53868): Good to Excellent
[Phosphate Buffered Saline 1X Sterile Solution]

PR1MA  PBS 1X Solution [Phosphate Buffered Saline 1X Sterile Solution]

Common Name: Phosphate Buffered Saline 1X Sterile Solution
CAS Number: 7647-14-5, 7447-40-7, 7558-79-4, 7778-77-0, 7732-18-5
Solubility: Water
Storage Temperature: Room Temperature


Phosphate Buffered Saline is a buffer solution commonly used in biological research. PBS has many uses because it is isotonic and non-toxic to most cells. These uses include substance dilution and cell container rinsing.


Ready to use sterile 1X solution.


Ingredients:

  • Sodium Chloride: 137 mM
  • Potassium Chloride: 2.7 mM
  • Phosphate Buffer: 11.9 mM
  • pH: 7.4
  • Does not contain Calcium or Magnesium


Research or further manufacturing use only, not for food or drug use.
 

  • Appearance: Clear and Colorless Solution
  • pH: 7.25 - 7.55

PR1MA  Doxycycline Hydrochloride

 
CAS Number: 10592-13-9
Molecular Weight: 480.9
Chemical Formula: C22H24N2O8  HCl
Solubility: Water
Storage Temperature: 2-8°C

 
Tetracycline antibiotic active against gram positive and gram negative bacteria. Interferes with bacterial cell wall synthesis.
 

Research or further manufacturing use only, not for food or drug use.
 
 

  • Appearance: Yellow crystalline powder
  • Absorbance (349nm): 300 - 335
  • Ethanol: 4.3 - 6.0 %
  • Heavy Metals :  50 ppm
  • Identification (A, B, C): Pass Test
  • Light Absorbing Impurities (490nm):  0.5
  • pH: 2.0 - 3.0
  • Potency : 800 - 920 µg/mg
  • Purity: 95.0 - 102.0 %
  • Related Compound (6-Epidoxycyline):  2.0 %
  • Related Substance (Any Other Impurity):  0.5 %
  • Specific Optical Rotation: -120 - -105 o
  • Sulfated Ash:  0.4 %
  • Water: 1.4 - 2.8 %

PR1MA  Metronidazole

CAS Number: 443-48-1
Molecular Weight: 171.2
Chemical Formula: C6H9N3O3
Solubility: Dilute acids
Storage Temperature: Room Temperature

 
Derivative of 5-Nitroimidazole with activity against anaerobic protozoa and anaerobic bacteria. Protect from light.

 
Soluble in:
Water (10 mg/ml at 20°C)
Ethanol (5 mg/ml)
Methanol
Chloroform (<0.5 mg/ml)
DMSO (34 mg/ml at 25°C)

 
WARNING! This product can expose you to Metronidazole, a chemical which is known to the State of California to cause cancer. For more information go to www.P65Warnings.ca.gov.

 
Research or further manufacturing use only, not for food or drug use.

  • Appearance: White or yellowish powder
  • Assay (Dry Basis): 99.0 - 101.0 %
  • Identification: Conform
  • Sulfated Ash:  0.1 %
  • Non Animal Origin: Non Animal Origin
  • Country of Origin : Report

PR1MA Acrylamide/bis-Acrylamide, 29:1 Ratio Solution

CAS Number: 79-06-1; 110-26-9; 7732-18-5
Storage Temperature: 2-8°C


A 40% solution containing 38.67% (w/v) Acrylamide and 1.33% (w/v) bis-Acrylamide for a monomer to crosslinker ratio of 29:1.


WARNING!
This product can expose you to Acrylamide, a chemical which is known to the State of California to cause cancer, developmental and reproductive harm. For more information go to www.P65Warnings.ca.gov.


Research or further manufacturing use only, not for food or drug use.


  • Appearance: Clear Colorless Solution
  • Conductivity of Solution:  10 µmhos
  • Electrophoresis: Pass Test
  • pH @25 oC: Report

[3',3",5',5"-Tetrabromo-phenolsulfonephthalein]

PR1MA Ammonium Acetate

CAS Number: 631-61-8
Molecular Weight: 77.08
Chemical Formula: C2H7NO2
Solubility: Water
Storage Temperature: 2-8°C


Widely used reagent in molecular biology and chromatography.


Research or further manufacturing use only, not for food or drug use.


  • Appearance: White crystalline hygroscopic powder
  • Assay:  97.0 %
  • Chloride (Cl):  0.0005 %
  • Heavy Metals (as Pb):  0.0005 %
  • Insoluble Matter:  0.005 %
  • Iron (Fe):  0.0005 %
  • Nitrate:  0.001 %
  • pH, 5% solution in H2O @25 ºC: 6.7 - 7.3
  • Residue after Ignition:  0.01 %
  • Sulfate:  0.001 %

PR1MA Phosphate Buffered Saline, 10X Solution

Common Name: Phosphate Buffered Saline 10X Solution
CAS Number: 7732-18-5; 7647-14-5; 7447-40-7; 7558-79-4; 7778-77-0
Solubility: Water
Storage Temperature: Room Temperature

Phosphate Buffered Saline is a buffer solution commonly used in biological research. PBS has many uses because it is isotonic and non-toxic to most cells. These uses include substance dilution and cell container rinsing.
Ready to use sterile 10X solution.

Research or further manufacturing use only, not for food or drug use.
  • Appearance: Clear and Colorless Solution
  • Composition: Sodium Chloride: 1.37M, Potassium Chloride: 0.027M, Phosphate Buffer: 0.12M
  • Method: Prepared in 18.2 megaohams-cm ± 1 water and filtered through 1 micron filter
  • pH (1X Solution): 7.2 - 7.6
  • pH (10X Solution): 6.8 - 7.2

PR1MA Acrylamide

CAS Number: 79-06-1
Molecular Weight: 71.1
Chemical Formula: C3H5NO
Solubility: Water
Storage Temperature: Room Temperature


WARNING!
This product can expose you to Acrylamide, a chemical which is known to the State of California to cause cancer, developmental and reproductive harm. For more information go to www.P65Warnings.ca.gov.


Research or further manufacturing use only, not for food or drug use.


  • Appearance: White Crystalline Powder
  • Acrylic Acid :  10 ppm
  • Assay:  99.9 %
  • Conductivity:  3.5 µmhos
  • DNase, RNase, protease: Not Detected
  • Electrophoresis: Pass Test
  • Iron:  1 ppm
  • Lead:  1 ppm
  • Magnesium:  1 ppm
  • Melting Point: 84.0 - 86.0 oC
  • pH (10%, 0.1 M NaCl @ 25 oC) : 6.5 - 7.5
  • Solubility (40% Aqueous Solution): Clear and colorless

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