Molecular Biology Reagents/Chemicals

MIDSCI™ gives you ultra-pure, cost-competitive molecular biology reagents and lab chemicals for life science research; often with significant cost savings over Sigma Aldrich and other lab reagent providers.

PR1MA™ Chemicals can help with a wide variety of research-based requirements.
 
Use our glycerol for polyacrylamide gel electrophoresis. Eliminate Agrobacterium after inoculation with the carbenicillin we carry. Or detect β-Galactosidase activity with X-GAL which, when combined with the IPTG we stock, can be used to detect lac gene expression in cloning applications.
 
We also carry Luria Broth (Lennox), for the propagation and maintenance of E. coli; DTT, in order to maintain –SH groups in a reduced state; and high gel strength agar for tissue culture and microbiological work.

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PR1MA  Spectinomycin Dihydrochloride Pentahydrate

CAS Number: 22189-32-8
Molecular Weight: 495.3
Chemical Formula: C14H24N2O7  2HCl  5H2O
Solubility: Water
Storage Temp: 2-8°C

Aminocyclitol antibiotic that inhibits protein synthesis. Cell culture tested.

Research or further manufacturing use only, not for food or drug use.

  • Appearance: White or almost white, Slightly hygroscopic powder
  • Identification: Pass Test
  • pH @25 °C: 3.8 - 5.6
  • Potency :  603 IU/mg
  • Residue after Ignition:  1.0 %
  • Solubility: Clear and colorless to light yellow
  • Specific Rotation: +15 - +21 °
  • Water (KF): 16.0 - 20.0 %

Proudly made in the USA! Click here for more made in America products!
 

PR1MA  Luria Agar Granulated [Miller's LB Agar]

Common Name: Miller's LB Agar
CAS Number: 91079-40-2; 8013-01-2; 7647-14-5; 9002-18-0
Solubility: Water with Heat
Storage Temp: Room Temperature

For propagation and maintenance of E. Coli.

Dissolve 37 grams in 1 Liter distilled water and adjust the pH to 7.2. Sterilize by autoclaving for 15 minutes.

Ingredients:

  • Tryptone: 10 g / L
  • Sodium Chloride: 10 g / L
  • Yeast Extract: 5 g / L
  • Agar: 12 g / L
Research or further manufacturing use only, not for food or drug use.
  • DCM Appearance: Homogeneous Free Flowing, Light Beige
  • PPM Appearance: Clear, none to light ppt, light yellow
  • pH (2.5 % Solution @25 oC): 6.8 - 7.2
  • Microorganism (E. coli ATCC 23724): Good to Excellent
  • Microorganism (E. coli ATCC 33694): Good to Excellent
  • Microorganism (E. coli ATCC 33849): Fair to Excellent
  • Microorganism (E. coli ATCC 39403): Good to Excellent
  • Microorganism (E. coli ATCC 47014): Good to Excellent
  • Microorganism (E. coli ATCC 53868): Good to Excellent

Proudly made in the USA! Click here for more made in America products!
 

PR1MA Mitomycin C

CAS Number: 50-07-7
Molecular Weight: 334.3
Chemical Formula: C15H18N4O5
Solubility: Ethanol
Storage Temp: 2-8°C
 

Antineoplastic antibiotic that acts as an alkylating agent after activation and also suppresses the synthesis of nucleic acids. A cell-cylce non specific agent, most active in the late G1 and early S phases.

WARNING! This product can expose you to Mitomycin-C, a chemical which is known to the State of California to cause cancer. For more information go to www.P65Warnings.ca.gov.

Research or further manufacturing use only, not for food or drug use.

  • Appearance: Blue violet solid
  • Identification (UV): Conforms
  • Purity-HPLC (RP CH3CN:H2O-0-100%):  99.0 %
  • Purity-HPLC (RP MEOH:H2O-90-10):  98.0 %
  • Purity-TLC (CH2CL2:MEOH- 9:1):  98.0 %
  • Solubility (10 mg/ml MEOH): Clear Blue Solution

Proudly made in the USA! Click here for more made in America products!
 


PR1MA DTT [DL-Dithiothreitol] (Cleland's Reagent)]

Common Name: [DL-Dithiothreitol] (Cleland's Reagent)]
CAS Number: 3483-12-3
Molecular Weight: 154.25
Chemical Formula: C4H10O2S2
Solubility: Water
Storage Temp: 2-8°C

Protective reagent for maintaining -SH groups in reduced state. DTT permeates the cell membrane, protecting protein sulfhydryls, thereby restoring enzyme activity lost by oxidation of the groups.

Research or further manufacturing use only, not for food or drug use.

  • Appearance: A white free flowing powder, hygroscopic
  • Solubility: Freely soluble in methanol, methylene chloride
  • Clarity in water: 5% (w/v) solution in water is clear and colorless
  • Identification by TLC: Rf value of the sample is comparable with that of the standard
  • Melting Range: 37.0 - 44.0 °C
  • Related Substance (UV Spectroscopy- Content of Oxidised DTT): 0.4 %
  • Assay (By Iodimetry):  98.0 %
  • Country of Origin: Report

Proudly made in the USA! Click here for more made in America products!
 


Sodium Sulfate

  • Appearance: White Crystalline Powder
  • Purity: ≥ 99.0%
  • pH (5% Aqueous): 5.2 - 9.2
  • Identification (FTIR): Conforms to Structure
  • Water: ≤ 0.5%
  • Insoluble Matter: ≤ 0.01%
  • Loss on Ignition: ≤ 0.05%
  • Chloride: ≤ 10 ppm
  • Nitrogen: ≤ 5 ppm
  • Phosphate: ≤ 10 ppm
  • Lead: ≤ 5 ppm
  • Iron: ≤ 10 ppm
  • DNase: None Detected
  • RNase: None Detected

*Custom sizes available upon request.

 

DOT Information: Non-regulated.   
 

Item#:
ASODIUMSULF
[N-(2-Hydroxyethyl) piperazine N'-(2-ethanesulfonic acid)]

PR1MA  HEPES, Free Acid [N-(2-Hydroxyethyl) piperazine N'-(2-ethanesulfonic acid)]

Common Name: [N-(2-Hydroxyethyl) piperazine N'-(2-ethanesulfonic acid)]
CAS Number: 7365-45-9
Molecular Weight: 238.3
Chemical Formula: C8H18N2O4S
Solubility: Water
Storage Temp: Room Temperature

Zwitterionic N-substituted aminosulfonic acid buffer.

  • pka (25°C): 7.5
  • pH Range (Working): 6.8 - 8.2
  • Heavy Metals: <0.001% Max
Research or further manufacturing use only, not for food or drug use.
  • Appearance: White Crystalline Powder
  • Assay (Titration, dried basis):  99.5 %
  • DNase, RNase, protease: Not Detected
  • Endotoxin (0.2% Solution):  0.25 EU/ml
  • Heavy Metals (as Pb):  0.001 %
  • Identification (IR): Pass
  • Iron (Fe):  0.0005 %
  • Loss on Drying:  1.0 %
  • pH (5% in water): 5.0 - 6.5
  • pKa @ 20°C: 7.35 - 7.70
  • Residue on Ignition:  0.1 %
  • Solubility (1M Solution in Water ): Clear and Colorless Solution
  • Sulfate:  0.05 %
  • UV Absorbance (1M@260nm):  0.05
  • UV Absorbance (1M@280nm):  0.05

Proudly made in the USA! Click here for more made in America products!
 


PR1MA Acrylamide/bis-Acrylamide, Premixed Powder 19:1 Ratio

Storage Temp: Room Temperature

Pre-measured powder blend of acrylamide and bis-acrylamide for simple stock solution preparation.

WARNING!
This product can expose you to Acrylamide, a chemical which is known to the State of California to cause cancer, developmental and reproductive harm. For more information go to www.P65Warnings.ca.gov.

Research or further manufacturing use only, not for food or drug use.

  • Appearance: Powder
  • Solubility: Clear and complete
  • Conductivity:  10 µmhos

Proudly made in the USA! Click here for more made in America products!
 

PR1MA Puromycin Dihydrochloride

CAS Number: 58-58-2
Molecular Weight: 544.43
Chemical Formula: C22H29N7O5 
 2HCl
Solubility: Water
Storage Temp: -20°C

Amino nucleoside antibiotic allows the selection of cells expressing the pac gene. Inhibits the growth of gram-positive bacteria and various animal and insect cells.

Research or further manufacturing use only, not for food or drug use.

  • Appearance: White to Off White Powder
  • Identification (TLC): Conforms
  • Melting Point: 168 - 182 oC
  • Purity (HPLC):  98.0 %
  • Solubility: Clear, colorless or light yellow solution
  • Water (KF):  12.0 %

Proudly made in the USA! Click here for more made in America products!
 

PR1MA Peptone, Soy 

CAS Number: 91079-46-8
Solubility: Water
Storage Temp: Room Temperature

From papaic hydrolysis of soybean meal. Low NaCl content.

Research or further manufacturing use only, not for food or drug use.

  • Amino Nitrogen:  2.2 %
  • Ash:  15 %
  • Loss on Drying:  6.0 %
  • pH (2% water) @25°C: 6.5 - 7.5
  • Total Nitrogen:  7.0 %
  • Coliform: Negative
  • E.Coli: Negative
  • Molds and Yeasts:  100 cfu/g
  • Salmonella: Negative /25g
  • Standard Plate Count :  5000 cfu/g
  • Broth Formulations (Growth Supporting Properties): Satisfactory
  • Peptone Agar (Growth Supporting Properties): Satisfactory
  • Country of Origin of Raw Material : Report
  • Raw Material Species: Plant
  • Country of Origin of Enzymes : Report
  • Enzyme Species: Porcine
  • Country Of Manufacture: Report

Proudly made in the USA! Click here for more made in America products!
 

Pall® Ampoule Media for Microbiological Analysis

  • Sterile Ampoule Media Pre-Measured for Maximum Efficiency and Convenience
  • Meets Membrane Filter (MF) Technique Standard Method requirements with 2 mL ampoules*
  • Maximizes efficiency with premixed and presterilized media
  • Choose between plastic or glass ampoules for your applications
  • Simplify pouring of the media with wide-mouth glass ampoules
  • All plastic ampoule media is economically packaged with 50 ampoules per box

Item#:
ASAMPOULEMEDIA


Sodium Chloride

  • Appearance: White Crystalline Powder
  • Purity: 99.0 - 101.5%
  • pH (5% Aqueous): 5.0 - 9.0
  • Lead: ≤ 5 ppm
  • Iron: ≤ 2 ppm
  • Insoluble Matter: ≤ 0.005%
  • Iodide: ≤ 20 ppm
  • Bromide: ≤ 100 ppm
  • Phosphate: ≤ 5 ppm
  • Sulfate: ≤ 40 ppm
  • Barium: To Pass Test
  • Calcium: ≤ 20 ppm
  • Magnesium: ≤ 10 ppm
  • Potassium: ≤ 50 ppm
  • Chlorate & Nitrate: ≤ 30 ppm
  • RNase: None Detected
  • DNase: None Detected

*Custom sizes available upon request.

 

DOT Information: Non-regulated.   
 

Item#:
ASCHLORIDESOD1

PR1MA LB Broth Powder [Lennox L Broth]

Common Name: Lennox L Broth
Solubility: Water
Storage Temp: Room Temperature

For propagation and maintenance of E. Coli. Low Salt Formula. Molecular Biology Grade.

Dissolve 20 grams in 1 liter distilled water. Then adjust pH to 7.2. Heat and frequently agitate to completely dissolve. Sterilize by autoclaving for 15 minutes.

Ingredients:

  • Tryptone: 10 g/L
  • Yeast Extract: 5 g/L
  • Sodium Chloride: 5 g/L

Research or further manufacturing use only, not for food or drug use.

  • DCM Appearance: Homogeneous free flowing, light beige
  • PPM Appearance: Clear, No to light ppt, light yellow
  • pH (2% water) @25°C: 6.8 - 7.2
  • Microorganism (E. coli ATCC 23724): Good to Excellent
  • Microorganism (E. coli ATCC 33694): Good to Excellent
  • Microorganism (E. coli ATCC 33849): Fair to Excellent
  • Microorganism (E. coli ATCC 39403): Good to Excellent
  • Microorganism (E. coli ATCC 47014): Good to Excellent
  • Microorganism (E. coli ATCC 53868): Good to Excellent
  • Sodium Chloride: 5 g/L
  • Yeast Extract: 5 g/L
  • Casein Peptone: 10 g/L

Proudly made in the USA! Click here for more made in America products!
 

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