Molecular Biology Reagents/Chemicals

MIDSCI™ gives you ultra-pure, cost-competitive molecular biology reagents and lab chemicals for life science research; often with significant cost savings over Sigma Aldrich and other lab reagent providers.

PR1MA™ Chemicals can help with a wide variety of research-based requirements.
 
Use our glycerol for polyacrylamide gel electrophoresis. Eliminate Agrobacterium after inoculation with the carbenicillin we carry. Or detect β-Galactosidase activity with X-GAL which, when combined with the IPTG we stock, can be used to detect lac gene expression in cloning applications.
 
We also carry Luria Broth (Lennox), for the propagation and maintenance of E. coli; DTT, in order to maintain –SH groups in a reduced state; and high gel strength agar for tissue culture and microbiological work.

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PR1MA  Doxycycline Hydrochloride

 
CAS Number: 10592-13-9
Molecular Weight: 480.9
Chemical Formula: C22H24N2O8  HCl
Solubility: Water
Storage Temperature: 2-8°C

 
Tetracycline antibiotic active against gram positive and gram negative bacteria. Interferes with bacterial cell wall synthesis.
 

Research or further manufacturing use only, not for food or drug use.
 
 

  • Appearance: Yellow crystalline powder
  • Absorbance (349nm): 300 - 335
  • Ethanol: 4.3 - 6.0 %
  • Heavy Metals :  50 ppm
  • Identification (A, B, C): Pass Test
  • Light Absorbing Impurities (490nm):  0.5
  • pH: 2.0 - 3.0
  • Potency : 800 - 920 µg/mg
  • Purity: 95.0 - 102.0 %
  • Related Compound (6-Epidoxycyline):  2.0 %
  • Related Substance (Any Other Impurity):  0.5 %
  • Specific Optical Rotation: -120 - -105 o
  • Sulfated Ash:  0.4 %
  • Water: 1.4 - 2.8 %

PR1MA  Metronidazole

CAS Number: 443-48-1
Molecular Weight: 171.2
Chemical Formula: C6H9N3O3
Solubility: Dilute acids
Storage Temperature: Room Temperature

 
Derivative of 5-Nitroimidazole with activity against anaerobic protozoa and anaerobic bacteria. Protect from light.

 
Soluble in:
Water (10 mg/ml at 20°C)
Ethanol (5 mg/ml)
Methanol
Chloroform (<0.5 mg/ml)
DMSO (34 mg/ml at 25°C)

 
WARNING! This product can expose you to Metronidazole, a chemical which is known to the State of California to cause cancer. For more information go to www.P65Warnings.ca.gov.

 
Research or further manufacturing use only, not for food or drug use.

  • Appearance: White or yellowish powder
  • Assay (Dry Basis): 99.0 - 101.0 %
  • Identification: Conform
  • Sulfated Ash:  0.1 %
  • Non Animal Origin: Non Animal Origin
  • Country of Origin : Report

PR1MA Acrylamide/bis-Acrylamide, 29:1 Ratio Solution

CAS Number: 79-06-1; 110-26-9; 7732-18-5
Storage Temperature: 2-8°C


A 40% solution containing 38.67% (w/v) Acrylamide and 1.33% (w/v) bis-Acrylamide for a monomer to crosslinker ratio of 29:1.


WARNING!
This product can expose you to Acrylamide, a chemical which is known to the State of California to cause cancer, developmental and reproductive harm. For more information go to www.P65Warnings.ca.gov.


Research or further manufacturing use only, not for food or drug use.


  • Appearance: Clear Colorless Solution
  • Conductivity of Solution:  10 µmhos
  • Electrophoresis: Pass Test
  • pH @25 oC: Report

[3',3",5',5"-Tetrabromo-phenolsulfonephthalein]

PR1MA Ammonium Acetate

CAS Number: 631-61-8
Molecular Weight: 77.08
Chemical Formula: C2H7NO2
Solubility: Water
Storage Temperature: 2-8°C


Widely used reagent in molecular biology and chromatography.


Research or further manufacturing use only, not for food or drug use.


  • Appearance: White crystalline hygroscopic powder
  • Assay:  97.0 %
  • Chloride (Cl):  0.0005 %
  • Heavy Metals (as Pb):  0.0005 %
  • Insoluble Matter:  0.005 %
  • Iron (Fe):  0.0005 %
  • Nitrate:  0.001 %
  • pH, 5% solution in H2O @25 ºC: 6.7 - 7.3
  • Residue after Ignition:  0.01 %
  • Sulfate:  0.001 %

PR1MA Phosphate Buffered Saline, 10X Solution

Common Name: Phosphate Buffered Saline 10X Solution
CAS Number: 7732-18-5; 7647-14-5; 7447-40-7; 7558-79-4; 7778-77-0
Solubility: Water
Storage Temperature: Room Temperature

Phosphate Buffered Saline is a buffer solution commonly used in biological research. PBS has many uses because it is isotonic and non-toxic to most cells. These uses include substance dilution and cell container rinsing.
Ready to use sterile 10X solution.

Research or further manufacturing use only, not for food or drug use.
  • Appearance: Clear and Colorless Solution
  • Composition: Sodium Chloride: 1.37M, Potassium Chloride: 0.027M, Phosphate Buffer: 0.12M
  • Method: Prepared in 18.2 megaohams-cm ± 1 water and filtered through 1 micron filter
  • pH (1X Solution): 7.2 - 7.6
  • pH (10X Solution): 6.8 - 7.2

 

PR1MA Agar, Granulated, High Gel Strength

CAS Number: 9002-18-0
Solubility: Water with Heat
Storage Temperature: Room Temperature


Purified agar with excellent properties for tissue culture and microbiological work.

  • To prepare media, add 15 grams to 1 liter of water.


Research or further manufacturing use only, not for food or drug use.


  • Appearance: White Cream Color, Average Particle Size Between 1-2 mm
  • Granulometry (ASTM) 60 Mesh Sieve Size: Report %
  • Ash:  6.5 %
  • E.Coli: Negative
  • Gel Point (1.5% Gel): 33 - 38°C
  • Gel Strength (1.5% Gel): 600 - 1100 g/cm2
  • Melting Point: 88 - 96°C
  • Moisture :  15 %
  • Molds and Yeasts: < 100 cfu/g
  • pH: 6.0 - 8.0
  • Total Plate Count: < 1000 cfu/g
  • Viscosity (1.5 Solution @ 60 oC):  30 cps
  • Water Absorption: ≤ 75 cc

PR1MA Acrylamide

CAS Number: 79-06-1
Molecular Weight: 71.1
Chemical Formula: C3H5NO
Solubility: Water
Storage Temperature: Room Temperature


WARNING!
This product can expose you to Acrylamide, a chemical which is known to the State of California to cause cancer, developmental and reproductive harm. For more information go to www.P65Warnings.ca.gov.


Research or further manufacturing use only, not for food or drug use.


  • Appearance: White Crystalline Powder
  • Acrylic Acid :  10 ppm
  • Assay:  99.9 %
  • Conductivity:  3.5 µmhos
  • DNase, RNase, protease: Not Detected
  • Electrophoresis: Pass Test
  • Iron:  1 ppm
  • Lead:  1 ppm
  • Magnesium:  1 ppm
  • Melting Point: 84.0 - 86.0 oC
  • pH (10%, 0.1 M NaCl @ 25 oC) : 6.5 - 7.5
  • Solubility (40% Aqueous Solution): Clear and colorless

Kappa is now PR1MA - Same great product, new name
Looking for something else? Email tech@midsci.com. If we can get it, we will add it to the website.

 

PR1MA Agarose, Low Melt Temperature

CAS Number: 9012-36-6
Solubility: Water
Storage Temperature: Room Temperature


Ideal gelling properties for use in cloning assays or for recovery of heat labile samples following electrophoresis.


Gel Strength, 1%: >200 g/cm2
Gelling Temperature: 26-30°C
Melting Temperature: <65°C
Sulfate: <0.10%
EEO: <0.10
DNase and RNase: None Detected


Research or further manufacturing use only, not for food or drug use.
 

  • Appearance: White to off white powder
  • DNase/ RNase: Not Detected
  • EEO:  0.10 -Mr
  • Gelling Temperature: 26 - 30 oC
  • Gel Strength :  200 g/cm2
  • Melting Temperature:  65 oC
  • Moisture Content:  10 %
  • Sulfate Content:  0.10 %

PR1MA Terrific Broth Modified Capsules

CAS Number: 91079-40-2; 8013-01-2; 7778-77-0; 7758-11-4
Solubility: Water
Storage Temperature: Room Temperature


Terrific Broth formulation packaged in easy to use 24 gram capsules. Formulation from powder.


Add 1 capsule per 500ml of water. Heat and stir until capsules are completely dissolved. Add 2ml of glycerol to mixture. Sterilize by autoclaving for 15 minutes.


Ingredients:

  • Casein Digest Peptone: 12 g/L
  • Yeast Extract: 24 g/L
  • Dipotassium Phosphate: 9.4 g/L
  • Potassium Phosphate: 2.2 g/L



Research or further manufacturing use only, not for food or drug use.


  • Appearance: Water soluble gelatin capsules containing cream to beige free flowing powder.
  • Appearance of Solution: Prepared medium is light to medium amber and clear
  • pH (4.76% solution at 25°C): 7.0 - 7.4
  • Microorganism (E. coli ATCC 23724): Good to Excellent
  • Microorganism (E. coli ATCC 33694): Good to Excellent
  • Microorganism (E. coli ATCC 33849): Good to Excellent
  • Microorganism (E. coli ATCC 39403): Good to Excellent
  • Microorganism (E. coli ATCC 47014): Good to Excellent
  • Microorganism (E. coli ATCC 53868): Good to Excellent
  • Yeast Extract: 24.0 g/L
  • Casein Peptone: 12.0 g/L
  • Potassium phosphate dibasic: 9.4 g/L
  • Potassium phosphate monobasic: 2.2 g/L
  • Direction (Preparation): Add 2 capsules to one liter of purified water until evenly dispersed. Add 4ml of Glycerol to the mixture. Boil for one minute with stirring to completely dissolve. Distribute and autoclave at 121°C for 15 minutes.

 

PR1MA LB Broth 20 Gram Buffered Capsules [Lennox L Broth]

Common Name: Lennox L Broth
Solubility: Water
Storage Temperature: Room Temperature


RPI media is now available in convenient premeasured and pre-mixed capsules. Prebuffered media eliminates pH adjustments, dust and smells from the lab. Low Salt Formula. Molecular Biology Grade.


For propagation and maintenance of E.Coli:

  • Add 1 capsule per liter of water.
  • Heat and stir until capsule is completely dissolved.
  • Buffer is set for 7.2 at 20°C using a 10mM solution of TRIS/TRIS HCl
  • Sterilize by autoclaving for 15 minutes.



Ingredients:

  • Tryptone: 10 g/L
  • Yeast Extract: 5 g/L
  • Sodium Chloride: 5 g/L



Research or further manufacturing use only, not for food or drug use.


  • Appearance: Capsules
  • Appearance of Solution: Prepared medium is yellow to gold and clear to moderately hazy
  • Microorganism (E. coli ATCC 23724): Good to Excellent
  • Microorganism (E. coli ATCC 33694): Good to Excellent
  • Microorganism (E. coli ATCC 33849): Fair to Excellent
  • Microorganism (E. coli ATCC 39403): Good to Excellent
  • Microorganism (E. coli ATCC 47014): Good to Excellent
  • Microorganism (E. coli ATCC 53868): Good to Excellent

PR1MA  Potassium Phosphate, Dibasic, Anhydrous

CAS Number: 7758-11-4
Molecular Weight: 174.2
Chemical Formula: K2HPO4
Solubility: Water
Storage Temperature: Room Temperature


Research or further manufacturing use only, not for food or drug use.


  • Appearance: White Powder
  • Assay:  98.0 %
  • Chloride:  0.003 %
  • Heavy Metals :  5 ppm
  • Insoluble Matter:  0.01 %
  • Iron (Fe):  0.001 %
  • Loss on Drying @150 oC:  1.0 %
  • Nitrogen Compound:  0.001 %
  • pH (5%): 8.5 - 9.6
  • Sodium (Na):  0.05 %
  • Sulfate:  0.005 %

PR1MA  Tetracycline Hydrochloride

CAS Number: 64-75-5
Molecular Weight: 480.9
Chemical Formula: C22H24N2O8  HCl
Solubility: Water
Storage Temperature: Room Temperature


Bacteriostatic antibiotic with activity against gram positive and gram negative bacteria. Slightly soluble in water.

 
WARNING! This product can expose you to Tetracycline HCl, a chemical which is known to the State of California to cause developmental harm. For more information go to www.P65Warnings.ca.gov.

 
Research or further manufacturing use only, not for food or drug use.

  • Appearance: Yellow, Crystalline Powder
  • Identification (A, B, C): Pass Test
  • Specific Rotation: -255 - - 240 °
  • Crystallinity: Meets with specification
  • pH (1% Solution): 1.8 - 2.8
  • Loss on Drying (105 oC):  2.0 %
  • Epitetracycline:  3.0 %
  • 4-Ephianhydrotetracyline:  2.0 %
  • Anhydrotetracycline:  0.5 %
  • 2-Acetyl-2-Decarbamoyltetracycline:  1.5 %
  • Chlortetracycline:  0.5 %
  • Sum of impurities other than epitetracycline:  5.0 %
  • Assay of Tetracycline Hydrochloride (On Dried Basis):  900 µg/mg
  • Residual Solvent (1-Butanol):  5000 ppm
  • Residual Solvent (Acetone):  500 ppm
  • Grade: USP
  • Country of Origin: Report
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