S2K Commerce - Products Dropdown
S2K Commerce - Shopping Cart
S2K Commerce - Order Entry
Molecular Biology Reagents/Chemicals
MIDSCI™ gives you ultra-pure, cost-competitive molecular biology reagents and lab chemicals for life science research; often with significant cost savings over Sigma Aldrich and other lab reagent providers.
PR1MA™ Chemicals can help with a wide variety of research-based requirements.
Use our glycerol for polyacrylamide gel electrophoresis. Eliminate Agrobacterium after inoculation with the carbenicillin we carry. Or detect β-Galactosidase activity with X-GAL which, when combined with the IPTG we stock, can be used to detect lac gene expression in cloning applications.
We also carry Luria Broth (Lennox), for the propagation and maintenance of E. coli; DTT, in order to maintain –SH groups in a reduced state; and high gel strength agar for tissue culture and microbiological work.
EZ Pack™ Agarose Tablets
- Eliminates weighing
- Fast dissolving, just two minutes
- Environmentally friendly, free from organic solvents
- Consistent, reproducible gels
- Enhanced resolution and clarity
Researchers trust Benchmark’s Agarose LE to meet their electrophoretic needs for various applications. Now, the same agarose is available in a convenient, no-mess tablet.
Agarose LE is refined in an advanced process that excludes the use of organic solvents. The result is a cleaner end product with significantly reduced environmental impact. The agarose can be used for analyses of nucleic acids from 150 bp to 25,000 bp, protein electrophoresis, and various blotting protocols.
The low EEO of the agarose promotes increased electrophoretic mobility, yielding improved resolution and shorter run times. This also allows macromolecules and larger particles (subcellular fragments, viruses, etc.) to migrate more freely through the gel matrix. The consistently low EEO reduces band distortion (caused by counterflow) that can result from the presence of excessive sulfate-rich negative ions. The quick-dissolving EZ Pack™ tablet contains 0.5 g (500 mg) of Agarose LE, eliminating the hands-on time and inaccuracies normally associated with weighing. Use of the tablet is not only convenient and cleaner, but it also provides better consistency and reproducibility gel to gel.
Gel preparation is simple – add the desired number of tablets to the electrophoresis buffer, allow it to sit for two minutes, and then heat and pour as usual. The resulting gels are highly transparent, have exceptional thermal stability (ensuring safe and easy handling), and exhibit exceptionally low absorption of chemical staining agents.
EZ Pack™ Agarose Tablets are supplied in convenient blister packs for safe, clean dispensing.
Specifications
Gel Temperature | 36°C ± 1.5°C (1.5%) |
Melt Temperature | 88°C ± 1.5°C (1.5%) |
EEO (-mr) | < 0.13 |
Moisture Content | < 10% |
Sulfate | < 0.2% |
RNase/DNase | None Detected |
Protease/Endonuclease | None Detected |
Storage Conditions | Room Temperature |
Gel Strength | > 1200 g / cm2 (1%) > 2500 g / cm2 (1.5%) |
Buffer volume required to achieve desired gel strength | |||||||
GEL % | 0.8% | 1.0% | 1.2% | 1.3% | 1.5% | 1.8% | 2.0% |
1 Tab | 63 mL | 50 mL | 42 mL | 38 mL | 33 mL | 28 mL | 25 mL |
2 Tabs | 125 mL | 100 mL | 83 mL | 77 mL | 67 mL | 56 mL | 50 mL |
3 Tabs | 188 mL | 150 mL | 125 mL | 115 mL | 100 mL | 83 mL | 75 mL |
Citric Acid, Anhydrous
- Appearance (Color): Colorless or White
- Appearance (Form): Crystals or Crystalline Powder
- Purity: ≥ 99.5%
- Identification (FTIR): Conforms to Structure
- Water: ≤ 0.3%
- Lead: ≤ 5 ppm
- Iron: ≤ 5 ppm
- Oxalate: ≤ 100 ppm
- Sulfate: ≤ 150 ppm
- Chloride: ≤ 50 ppm
- Barium: Passes Test
- Residue on Ignition: ≤ 0.05%
- Readily Carbonizable Substances: Passes Test
- Bacterial Endotoxins: ≤ 0.5 IU/mg
- DNase: None Detected
- RNase: None Detected
*Custom sizes available upon request.
DOT Information: Non-regulated.
Sodium Phosphate Dibasic, Anhydrous
- Appearance: White Crystalline Powder
- Purity: ≥ 98.0%
- pH (1% Aqueous): 8.4 – 9.6
- Solubility: Clear and Colorless
- Identity (FTIR): Conforms to Structure
- Loss on Drying (105°C / 4h): ≤ 0.5%
- Insoluble Matter: ≤ 0.2%
- Phosphate: 49.0 - 51.0%
- Fluoride: ≤ 10 ppm
- Arsenic: ≤ 1 ppm
- Lead: ≤ 1 ppm
- RNase: None Detected
- DNase: None Detected
*Custom sizes available upon request.
DOT Information: Non-regulated.
Bullseye Agarose General Purpose
Bullseye Molecular Biology Grade Agarose is certified for use in standard electrophoresis procedures, including nucleic acid analysis, preparative electrophoresis, and blotting. This highly refined and purified agarose is produced under strict testing parameters to ensure lot-to-lot consistency. It features low electrophoretic mobility characteristics, making it ideal for a variety of applications.
Extracted from the cell walls of certain Rhodophyceae (agarophyte seaweeds), Bullseye Standard Agarose is a neutral polysaccharide composed of galactan, linked by agarobioses in a 1-3, 1-4 arrangement. This unique chemical structure enables agarose to form robust gels even at low concentrations. The gels exhibit a macroreticular structure with an open mesh, easily adjustable by varying the agarose concentration.
Bullseye Agarose GP2 offers:
- General-purpose electrophoresis and blotting capabilities
- Excellent separation, resolution, and clarity
- Low absorption and background from staining reagents
- High gel strength for safe and easy handling
- Superior refinement and purification
- Enhanced thermal stability
- Comprehensive packaging including a specification sheet, preparation instructions, and MSDS
- Choose Bullseye Standard Agarose for reliable performance in your molecular biology applications.
Physical Specifications:
CAS#: 9012-36-6
Moisture: ≤ 7%
Ash: ≤ 0.4%
EEO: 0.05-0.13
Sulfate: ≤ 0.10%
Clarity @ 1.5% (NTU): ≤ 3
Gel Strength @ 1.0% (g/cm2): ≥ 1200
Gel Strength @ 1.5% (g/cm2): ≥ 2500
Gelling Temperature @ 1.5%: 36°C ± 1.5°C
Melting Temperature @ 1.5%: 88°C ± 1.5°C
Molecular Biology Specifications:
DNase assay: None Detected
RNase assay: None Detected
Protease assay: None Detected
Gel analysis assay: Pass
DNA Resolution: ≥ 1000bp (Finely Resolved)
DNA Extraction assay: Pass
Gel Background: Very Low
Base Pair Data | ||
1X TAE Buffer | Gel Concentration % | 1X TBE Buffer |
50-1500bp | 2 | 50-1,000bp |
100-2500bp | 1.75 | 100-2,000bp |
200-5000bp | 1.5 | 150-3,000bp |
250-10,000bp | 1.25 | 200-4,000bp |
300-16,000bp | 1 | 300-8,000bp |
500-20,000bp | 0.75 | 500-12,000bp |
Sodium Phosphate Monobasic, Monohydrate
- Appearance (Color): White to Off-White
- Appearance (Form): Crystalline Powder
- Purity: ≥ 99.0%
- pH (5% Aqueous): 8.7 - 9.3
- Identity (FTIR): Conforms to Structure
- Loss on Drying (105°C/4h): 10.0 - 15.0%
- Insoluble Matter: ≤ 0.2%
- Lead: ≤ 4 ppm
- Arsenic: ≤ 3 ppm
- Phosphorous: 20.0 - 24.0%
- Chloride: ≤ 140 ppm
- Fluoride: ≤ 50 ppm
- Sulfate: ≤ 150 ppm
- RNase: None Detected
- DNase: None Detected
*Custom sizes available upon request.
DOT Information: Non-regulated.
Dextrose (D-Glucose)
- Appearance (Color): White to Off-White
- Appearance (Form): Crystalline Powder
- Purity: ≥ 99.5%
- Solubility (2 g / 15 mL): Clear and Colorless
- Identity (FTIR): Conforms to Structure
- Conductivity: ≤ 20 uS / cm
- Specific Rotation: 52.6° to 53.2°
- Water: ≤ 1.0%
- Lead: ≤ 0.5 ppm
- Sulfates: ≤ 200 ppm
- Chloride: ≤ 125 ppm
- Sulfated Ash: ≤ 0.1%
- Calcium: ≤ 200 ppm
- Endotoxins: ≤ 1.25 EU/g
- RNase: None Detected
- DNase: None Detected
*Custom sizes available upon request.
DOT Information: Non-regulated.
PR1MA™ Agarose 3:1, Molecular Biology Grade
CAS Number: 9012-36-6
Solubility: Water
Storage Temp: Room Temperature
Molecular Biology Grade. For DNA/RNA fragments 150-1500bp. Exceptional gel strength. DNase/RNase free.
Gel Strength, 4%: ≥ 1400 g/cm2
Gelling Temperature (4%): 32-28°C
Melting Temperature (4%): 90°C
Sulfate: <0.15%
EEO: <0.13
Research or further manufacturing use only, not for food or drug use.
- Appearance: White to Off White Powder
- EEO: ≤ 0.13 -Mr
- Gelling Temperature: 32.5 - 38.0 oC
- Gel Strength : ≥ 1400 g/cm2
- Melting Temperature: ≤ 90.0 oC
- Moisture Content: ≤ 10.0 %
- Sulfate: ≤ 0.15 %
Proudly made in the USA! Click here for more made in America products!
PR1MA™ TRIS Hydrochloride [Tris(hydroxymethyl) aminomethane HCl]
Common Name: Tris(hydroxymethyl) aminomethane hydrochloride
CAS Number: 1185-53-1
Molecular Weight: 157.6
Chemical Formula: C4H11NO3 • HCl
Solubility: Water
Storage Temp: Room Temperature
Highly purified for use in molecular biology applications.
Research or further manufacturing use only, not for food or drug use.
- Appearance: White crystalline
- Assay: 99.0 - 101.0 %
- pH (1% aqueous solution): 4.2 - 5.0
- Solubility (1M Solution in Water ) : Clear, colorless solution
- UV Absorbance @260nm, 1.0 M in H2O: ≤ 0.06
- UV Absorbance @280nm, 1.0 M in H2O: ≤ 0.05
- Identification: Conforms
- Heavy Metals : ≤ 5 ppm
- Grade: USP
Proudly made in the USA! Click here for more made in America products!
PR1MA™ Nystatin
CAS Number: 1400-61-9
Molecular Weight: 926.1
Chemical Formula: C47H75NO17
Solubility: DMSO
Storage Temp: 2-8°C
Polyene antifungal antibiotic produced by Streptomyces noursei.
Research or further manufacturing use only, not for food or drug use.
- Appearance: Yellow to light tan powder
- Crystallinity: Pass Test
- Identification: Pass Test
- Loss on Drying: ≤ 5.0 %
- pH @25 °C: 6.0 - 8.0
- Potency (Assay): ≥ 4400 U/mg
- Suspendability: Pass Test
- Residual Solvent (n-butanol): ≤ 1000 ppm
- Residual Solvent (Methanol): ≤ 3000 ppm
- Residual Solvent (Acetone): ≤ 5000 ppm
- Residual Solvents: The following Class 1,2, or 3 Solvents may be present in this product. All comply with the limits prescribed in the current USP.
Proudly made in the USA! Click here for more made in America products!
PR1MA™ MES Monohydrate [2-(N-Morpholino)-ethanesulfonic acid]
Common Name: 2-(N-Morpholino)ethanesulfonic acid
CAS Number: 145224-94-8
Molecular Weight: 213.24
Chemical Formula: C6H13NO4S • H 2O
Solubility: Water
Storage Temp: Room Temperature
Buffer for use in plant tissue culture.
- pKa (20°C): 6.15
- pH range: 5.5 - 6.7
Research or further manufacturing use only, not for food or drug use.
- Appearance: White crystalline powder
- Assay (Titration): ≥ 99.0 %
- Heavy Metals: ≤ 0.0010 %
- Loss on Drying: 7.0 - 10.0 %
- pH: 2.5 - 4.0
- pKa @20 oC: 6.02 - 6.25
- Solubility (10% solution in H2O): Clear
- Sulfate: ≤ 0.01 %
- UV Absorbance 250nm in 10% H2O: ≤ 0.05
- Country of Origin: Report
Proudly made in the USA! Click here for more made in America products!
PR1MA™ bis-Acrylamide, 2% Solution
CAS Number: 110-26-9
Storage Temp: 2-8°C
A 2% (w/v) bis-Acrylamide solution.
Research or further manufacturing use only, not for food or drug use.
- Appearance: Clear, colorless liquid
- Conductivity of Solution: Report µmhos
- Electrophoresis: Pass Test
- pH: Report
Proudly made in the USA! Click here for more made in America products!
PR1MA™ Potassium Acetate
CAS Number: 127-08-2
Molecular Weight: 98.14
Chemical Formula: C2H3O2K
Solubility: Water
Storage Temp: Room Temperature
Research or further manufacturing use only, not for food or drug use.
- Appearance: White crystalline powder
- Calcium (Ca): ≤ 0.005 %
- Chloride (Cl): ≤ 0.003 %
- Heavy Metals : ≤ 5 ppm
- Insolubles: ≤ 0.005 %
- Iron: ≤ 5 ppm
- Magnesium (Mg): ≤ 0.002 %
- pH (5% in water): 6.5 - 9.0
- Phosphate: ≤ 0.001 %
- Purity: ≥ 99.0 %
- Sodium (Na): ≤ 0.03 %
- Sulfate: ≤ 0.002 %
- Country of Origin: Report