Molecular Biology Reagents/Chemicals

MIDSCI™ gives you ultra-pure, cost-competitive molecular biology reagents and lab chemicals for life science research; often with significant cost savings over Sigma Aldrich and other lab reagent providers.

PR1MA™ Chemicals can help with a wide variety of research-based requirements.
 
Use our glycerol for polyacrylamide gel electrophoresis. Eliminate Agrobacterium after inoculation with the carbenicillin we carry. Or detect β-Galactosidase activity with X-GAL which, when combined with the IPTG we stock, can be used to detect lac gene expression in cloning applications.
 
We also carry Luria Broth (Lennox), for the propagation and maintenance of E. coli; DTT, in order to maintain –SH groups in a reduced state; and high gel strength agar for tissue culture and microbiological work.

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Sodium Phosphate Dibasic, Anhydrous

  • Appearance: White Crystalline Powder
  • Purity: ≥ 98.0%
  • pH (1% Aqueous): 8.4 – 9.6
  • Solubility: Clear and Colorless
  • Identity (FTIR): Conforms to Structure
  • Loss on Drying (105°C / 4h): ≤ 0.5%
  • Insoluble Matter: ≤ 0.2%
  • Phosphate: 49.0 - 51.0%
  • Fluoride: ≤ 10 ppm
  • Arsenic: ≤ 1 ppm
  • Lead: ≤ 1 ppm
  • RNase: None Detected
  • DNase: None Detected

*Custom sizes available upon request.

 

DOT Information: Non-regulated.
 

Item#:
ASPHOSODANH1

Bullseye Agarose General Purpose

 

Bullseye Molecular Biology Grade Agarose is certified for use in standard electrophoresis procedures, including nucleic acid analysis, preparative electrophoresis, and blotting. This highly refined and purified agarose is produced under strict testing parameters to ensure lot-to-lot consistency. It features low electrophoretic mobility characteristics, making it ideal for a variety of applications.

 

Extracted from the cell walls of certain Rhodophyceae (agarophyte seaweeds), Bullseye Standard Agarose is a neutral polysaccharide composed of galactan, linked by agarobioses in a 1-3, 1-4 arrangement. This unique chemical structure enables agarose to form robust gels even at low concentrations. The gels exhibit a macroreticular structure with an open mesh, easily adjustable by varying the agarose concentration.

 

Bullseye Agarose GP2 offers:

  • General-purpose electrophoresis and blotting capabilities
  • Excellent separation, resolution, and clarity
  • Low absorption and background from staining reagents
  • High gel strength for safe and easy handling
  • Superior refinement and purification
  • Enhanced thermal stability
  • Comprehensive packaging including a specification sheet, preparation instructions, and MSDS
  • Choose Bullseye Standard Agarose for reliable performance in your molecular biology applications.

 

 

Physical Specifications:

CAS#: 9012-36-6
Moisture: ≤ 7%
Ash: ≤ 0.4%
EEO: 0.05-0.13
Sulfate: ≤ 0.10%
Clarity @ 1.5% (NTU): ≤ 3
Gel Strength @ 1.0% (g/cm2): ≥ 1200
Gel Strength @ 1.5% (g/cm2): ≥ 2500
Gelling Temperature @ 1.5%: 36°C ± 1.5°C
Melting Temperature @ 1.5%: 88°C ± 1.5°C

 

 

Molecular Biology Specifications:

DNase assay: None Detected
RNase assay: None Detected
Protease assay: None Detected
Gel analysis assay: Pass
DNA Resolution: ≥ 1000bp (Finely Resolved)
DNA Extraction assay: Pass
Gel Background: Very Low

 

 

Base Pair Data
1X TAE BufferGel Concentration %1X TBE Buffer
50-1500bp250-1,000bp
100-2500bp1.75100-2,000bp
200-5000bp1.5150-3,000bp
250-10,000bp1.25200-4,000bp
300-16,000bp1300-8,000bp
500-20,000bp0.75500-12,000bp
Item#:
ASPCRAGAROSE1


Sodium Phosphate Monobasic, Monohydrate

  • Appearance (Color): White to Off-White
  • Appearance (Form): Crystalline Powder
  • Purity: ≥ 99.0%
  • pH (5% Aqueous): 8.7 - 9.3
  • Identity (FTIR): Conforms to Structure
  • Loss on Drying (105°C/4h): 10.0 - 15.0%
  • Insoluble Matter: ≤ 0.2%
  • Lead: ≤ 4 ppm
  • Arsenic: ≤ 3 ppm
  • Phosphorous: 20.0 - 24.0%
  • Chloride: ≤ 140 ppm
  • Fluoride: ≤ 50 ppm
  • Sulfate: ≤ 150 ppm
  • RNase: None Detected
  • DNase: None Detected 

*Custom sizes available upon request.

 

DOT Information: Non-regulated.   
 

Item#:
ASPHOSODMONMON


Dextrose (D-Glucose)

  • Appearance (Color): White to Off-White
  • Appearance (Form): Crystalline Powder
  • Purity: ≥ 99.5%
  • Solubility (2 g / 15 mL): Clear and Colorless
  • Identity (FTIR): Conforms to Structure
  • Conductivity: ≤ 20 uS / cm
  • Specific Rotation: 52.6° to 53.2°
  • Water: ≤ 1.0%
  • Lead: ≤ 0.5 ppm
  • Sulfates: ≤ 200 ppm
  • Chloride: ≤ 125 ppm
  • Sulfated Ash: ≤ 0.1%
  • Calcium: ≤ 200 ppm
  • Endotoxins: ≤ 1.25 EU/g
  • RNase: None Detected
  • DNase: None Detected

*Custom sizes available upon request.

 

DOT Information: Non-regulated.   
 

Item#:
ASDEXANHY

PR1MA Agarose 3:1, Molecular Biology Grade

CAS Number: 9012-36-6
Solubility: Water
Storage Temp: Room Temperature

Molecular Biology Grade. For DNA/RNA fragments 150-1500bp. Exceptional gel strength. DNase/RNase free.

Gel Strength, 4%: ≥ 1400 g/cm2
Gelling Temperature (4%): 32-28°C
Melting Temperature (4%): 90°C
Sulfate: <0.15%
EEO: <0.13

Research or further manufacturing use only, not for food or drug use.

  • Appearance: White to Off White Powder
  • EEO:  0.13 -Mr
  • Gelling Temperature: 32.5 - 38.0 oC
  • Gel Strength :  1400 g/cm2
  • Melting Temperature:  90.0 oC
  • Moisture Content:  10.0 %
  • Sulfate:  0.15 %


Proudly made in the USA! Click here for more made in America products!

PR1MA  TRIS Hydrochloride [Tris(hydroxymethyl) aminomethane HCl]

Common Name: Tris(hydroxymethyl) aminomethane hydrochloride
CAS Number: 1185-53-1
Molecular Weight: 157.6
Chemical Formula: C4H11NO3  HCl
Solubility: Water
Storage Temp: Room Temperature

Highly purified for use in molecular biology applications.

Research or further manufacturing use only, not for food or drug use.

  • Appearance: White crystalline
  • Assay: 99.0 - 101.0 %
  • pH (1% aqueous solution): 4.2 - 5.0
  • Solubility (1M Solution in Water ) : Clear, colorless solution
  • UV Absorbance @260nm, 1.0 M in H2O:  0.06
  • UV Absorbance @280nm, 1.0 M in H2O:  0.05
  • Identification: Conforms
  • Heavy Metals :  5 ppm
  • Grade: USP

Proudly made in the USA! Click here for more made in America products!
 

PR1MA Nystatin

CAS Number: 1400-61-9
Molecular Weight: 926.1
Chemical Formula: C47H75NO17
Solubility: DMSO
Storage Temp: 2-8°C

Polyene antifungal antibiotic produced by Streptomyces noursei.

Research or further manufacturing use only, not for food or drug use.

  • Appearance: Yellow to light tan powder
  • Crystallinity: Pass Test
  • Identification: Pass Test
  • Loss on Drying:  5.0 %
  • pH @25 °C: 6.0 - 8.0
  • Potency (Assay):  4400 U/mg
  • Suspendability: Pass Test
  • Residual Solvent (n-butanol):  1000 ppm
  • Residual Solvent (Methanol):  3000 ppm
  • Residual Solvent (Acetone):  5000 ppm
  • Residual Solvents: The following Class 1,2, or 3 Solvents may be present in this product. All comply with the limits prescribed in the current USP.

Proudly made in the USA! Click here for more made in America products!
 

PR1MA  MES Monohydrate [2-(N-Morpholino)-ethanesulfonic acid]

Common Name: 2-(N-Morpholino)ethanesulfonic acid
CAS Number: 145224-94-8
Molecular Weight: 213.24
Chemical Formula: C6H13NO4 H 2O
Solubility: Water
Storage Temp: Room Temperature

Buffer for use in plant tissue culture.

  • pKa (20°C): 6.15
  • pH range: 5.5 - 6.7

Research or further manufacturing use only, not for food or drug use.

  • Appearance: White crystalline powder
  • Assay (Titration):  99.0 %
  • Heavy Metals:  0.0010 %
  • Loss on Drying: 7.0 - 10.0 %
  • pH: 2.5 - 4.0
  • pKa @20 oC: 6.02 - 6.25
  • Solubility (10% solution in H2O): Clear
  • Sulfate:  0.01 %
  • UV Absorbance 250nm in 10% H2O:  0.05
  • Country of Origin: Report

Proudly made in the USA! Click here for more made in America products!
 


PR1MA bis-Acrylamide, 2% Solution

CAS Number: 110-26-9
Storage Temp: 2-8°C

A 2% (w/v) bis-Acrylamide solution.

Research or further manufacturing use only, not for food or drug use.

  • Appearance: Clear, colorless liquid
  • Conductivity of Solution: Report µmhos
  • Electrophoresis: Pass Test
  • pH: Report

Proudly made in the USA! Click here for more made in America products!
 

PR1MA Potassium Acetate

CAS Number: 127-08-2
Molecular Weight: 98.14
Chemical Formula: C2H3O2K
Solubility: Water
Storage Temp: Room Temperature

Research or further manufacturing use only, not for food or drug use.

  • Appearance: White crystalline powder
  • Calcium (Ca):  0.005 %
  • Chloride (Cl):  0.003 %
  • Heavy Metals :  5 ppm
  • Insolubles:  0.005 %
  • Iron:  5 ppm
  • Magnesium (Mg):  0.002 %
  • pH (5% in water): 6.5 - 9.0
  • Phosphate:  0.001 %
  • Purity:  99.0 %
  • Sodium (Na):  0.03 %
  • Sulfate:  0.002 %
  • Country of Origin: Report

Proudly made in the USA! Click here for more made in America products!
 


PR1MA  Blasticidin S Hydrochloride 10 mg / mL Solution

CAS Number: 3513-03-9
Molecular Weight: 458.9
Chemical Formula: C17H26N8O5  HCl
Solubility: Water
Storage Temp: -20°C

A peptidyl nucleoside antibiotic isolated from the culture broth of Streptomyces griseogenes, inhibits protein synthesis in both prokaryotes and eukaryates.

Supplied as a 10 mg / mL sterile solution in HEPES buffer.
Research or further manufacturing use only, not for food or drug use.
  • Appearance: Clear Solution
  • Concentration: 10mg/ml sterile solution
  • Description: Colorless solution in HEPES buffer
  • pH: 7.2 - 7.5
  • Purity:  98.0 %

Proudly made in the USA! Click here for more made in America products!
 

PR1MA Calcium Chloride Anhydrous

CAS Number: 10043-52-4
Molecular Weight: 110.98
Chemical Formula: CaCl2
Solubility: Water
Storage Temp: Room Temperature

Research or further manufacturing use only, not for food or drug use.

  • Appearance: White crystalline powder
  • Assay (Titration):  96.0 %
  • Titratable Base:  0.006 meq/g

Proudly made in the USA! Click here for more made in America products!


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