Molecular Biology Reagents/Chemicals

MIDSCI™ gives you ultra-pure, cost-competitive molecular biology reagents and lab chemicals for life science research; often with significant cost savings over Sigma Aldrich and other lab reagent providers.

PR1MA™ Chemicals can help with a wide variety of research-based requirements.
 
Use our glycerol for polyacrylamide gel electrophoresis. Eliminate Agrobacterium after inoculation with the carbenicillin we carry. Or detect β-Galactosidase activity with X-GAL which, when combined with the IPTG we stock, can be used to detect lac gene expression in cloning applications.
 
We also carry Luria Broth (Lennox), for the propagation and maintenance of E. coli; DTT, in order to maintain –SH groups in a reduced state; and high gel strength agar for tissue culture and microbiological work.

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PR1MA  Paromomycin Sulfate

CAS Number: 1263-89-4
Molecular Weight: 615.6
Chemical Formula: C23H45N5O14  H2SO4
Solubility: Water
Storage Temperature: Room Temperature


Aminoglycoside antibiotic with mode of action similar to Kanamycin and Neomycin. Used as a selective agent for the incorporation of the NPT II (APH3') gene in plant tissue. Plant culture tested.


Research or further manufacturing use only, not for food or drug use.


  • Appearance: White to Off White Powder
  • Assay: ≥ 675 U/mg
  • Identification: Conforms
  • Loss on Drying:  5.0 %
  • pH 3.0% Solution @25oC: 5.0 -7.5
  • Specific Optical Rotation: +50.0 - +55.0 °
Premixed Powder 29:1 Ratio

PR1MA Acrylamide/bis-Acrylamide, Premixed Powder 29:1 Ratio

Storage Temperature: Room Temperature


Pre-measured powder blend of acrylamide and bis-acrylamide for simple stock solution preparation.


WARNING!
This product can expose you to Acrylamide, a chemical which is known to the State of California to cause cancer, developmental and reproductive harm. For more information go to www.P65Warnings.ca.gov.


Research or further manufacturing use only, not for food or drug use.


  • Appearance: White Powder
  • Conductivity of Solution: ≤ 10.0 µmhos
  • Electrophoresis: Pass Test
  • Solubility: Pass Test

  • RNase & DNase Free Water A new process of purifying large quantity of DNase and RNase free water enables us to offer Molecular Grade Water at a price that any laboratory can afford. 

  • No toxic agents such as DEPC are used in making the Molecular Grade Water; therefore, there is no chance of DEPC interfering with enzymatic reactions. 

  • The Molecular Grade Water is suitable for use in molecular biology applications which demand a high quality of water and assurance that the water is free from DNase and RNase contamination. 

  • Molecular Grade Water can be used for making reagents and rinsing glass/plastic wares for DNA and RNA research. 

  • Each lot is quality tested for the absence of RNase and DNase contamination. Test reports are attached with each container as an added assurance.
Item#:
ASMOLWATER2

PR1MA  SOB Broth, Capsules

CAS Number: 91079-40-2; 8013-01-2; 7647-14-5; 7487-88-9; 7447-40-7
Solubility: Water
Storage Temperature: Room Temperature


SOB Broth is recommended for use in cultivation of recombinant strains of E. Coli. It is a nutritionally rich growth medium for use in the preparation and transformation of competent cells.


Add two capsules to one liter of distilled water. Heat and stir gently to completely dissolve medium. Sterilize by autoclaving at 121°C for 15 minutes.


Final Solution contains:

  • Casein Enzymic Hydrolysate: 20 g/L
  • Yeast Extract: 5 g/L
  • Sodium Chloride: 0.5 g/L
  • Magnesium Sulfate: 2.4 g/L
  • Potassium Chloride: 0.186 g/L



Research or further manufacturing use only, not for food or drug use.


  • Appearance: Capsules
  • Appearance of Solution: Prepared medium is light to medium amber and clear
  • pH @25 °C: 6.8 - 7.2
  • Microorganism (E. coli ATCC 23724): Good to Excellent
  • Microorganism (E. coli ATCC 33694): Good to Excellent
  • Microorganism (E. coli ATCC 33849): Good to Excellent
  • Microorganism (E. coli ATCC 39403): Good to Excellent
  • Microorganism (E. coli ATCC 47014): Good to Excellent
  • Microorganism (E. coli ATCC 53868): Good to Excellent
[NTC] [clonNAT] [Streptothricin Sulfate]

PR1MA  Nourseothricin Sulfate [NTC] [clonNAT] [Streptothricin Sulfate]

Common Name: [NTC] [clonNAT] [Streptothricin Sulfate]
CAS Number: 96736-11-7
Solubility: Water
Storage Temperature: 2-8°C


Dihydrogen sulfate form of the weekly basic antibiotic Nourseothricin consisting of components F, E and D. Effective against gram negative and gram positive bacteria. Also effective against mycobacteria, mycoplasms, protozoa, yeasts, viruses and plants. Commonly used as a selection agent for recombinant yeast strains.


Research or further manufacturing use only, not for food or drug use.
 

  • Appearance: White Powder
  • Carbon (C): Report %
  • Hydrogen (H): Report %
  • Nitrogen (N): Report %
  • Purity (HPLC):  85 %
  • Sulfur (S): Report %

PR1MA™  Bacitracin

CAS Number: 1405-87-4
Molecular Weight: 1422.69
Chemical Formula: C66H103N17O16 S
Solubility: Water, Ethanol, and Methanol
Storage Temperature: 2-8°C


Interferes with bacterial cell wall synthesis. Active against gram positive bacteria. Toxic to plant cells.


Research or further manufacturing use only, not for food or drug use.
 
 

  • Appearance: White to pale buff powder
  • Identification: Pass test
  • pH: 5.5 - 7.5
  • Loss on Drying:  5.0 %
  • Residue on Ignition:  0.5 %
  • Bacitracin A:  40.0 %
  • SUM of bacitracin A, B1, B2 and B3:  70.0 %
  • Limit of early eluting peptides:  20.0 %
  • Limit of Bacitracin F:  6.0 %
  • Microbial Assay (Dried Basis):  65.0 IU/mg
  • Heavy Metals :  30 ppm
  • Total Viable Aerobic Count:  100 CFU/g
  • Particle Size (< 20µm by volume):  99.0 %
  • Crystallinity : Pass
  • Appearance of Solution: Clear, colorless to slight hazy yellow
  • Source: Bacillus Licheniformis
  • Non Animal Origin: Non Animal Origin
  • Grade: USP
  • Country of Origin : Report

PR1MA bis-TRIS [bis-(2-hydroxyethyl)aminotris(hydroxymethyl)methane]

Common Name: bis(2-Hydroxyethyl)aminotris (hydroxymethyl)methane
CAS Number: 6976-37-0
Molecular Weight: 209.2
Chemical Formula: C8H19NO5
Solubility: Water
Storage Temperature: Room Temperature


 
Research or further manufacturing use only, not for food or drug use.
 

  • Appearance: White crystalline powder
  • Assay:  99.0 %
  • Identification (IR): Matches reference
  • Melting Point: Report oC
  • pH, 1.0M Aqueous solution @25ºC: 9.5 - 11.0
  • pKa @ 20°C: 6.0 - 7.0
  • Solubility: Complete and clear
  • UV Absorbance @280nm, 1.0 M in H2O:  0.40

 

PR1MA Citric Acid Anhydrous

CAS Number: 77-92-9
Molecular Weight: 192.1
Chemical Formula: C6H8O7
Solubility: Water
Storage Temperature: Room Temperature


Research or further manufacturing use only, not for food or drug use.


  • Appearance: Fine white crystalline powder
  • Assay (Titration):  99.5 %
  • Carbonizable Substances (H2SO4): Pass Test
  • Clarity and Color: Pass Test
  • Identification: Pass Test
  • Lead :  0.5 mg/kg
  • Limit of Oxalic Acid:  0.036 %
  • Oxalate Test: Pass Test
  • Residual Solvents: Meet requirements
  • Residue on Ignition:  0.05 %
  • Sulfate:  0.015 %
  • Water:  0.5 %
Premixed Powder 37.5:1 Ratio

PR1MA Acrylamide/bis-Acrylamide, Premixed Powder 37.5:1 Ratio

CAS Number: 79-06-1, 110-26-9
Storage Temperature: Room Temperature


Pre-measured powder blend of acrylamide and bis-acrylamide for simple stock solution preparation.


WARNING!
This product can expose you to Acrylamide, a chemical which is known to the State of California to cause cancer, developmental and reproductive harm. For more information go to www.P65Warnings.ca.gov.


Research or further manufacturing use only, not for food or drug use.


  • Appearance: White powder
  • Conductivity of Solution:  10 µmhos
  • Electrophoresis: Pass Test
  • Solubility: Pass Test


PR1MA LB Agar, Low Salt Formula, Powder [Lennox L Agar]

Common Name: Lennox L Agar
CAS Number: 91079-40-2, 8013-01-2, 7647-14-5, 9002-18-0
Solubility: Water with Heat
Storage Temperature: Room Temperature


Molecular Biology Grade. For propagation and maintenance of E. Coli. Low Salt Formula.


Dissolve 35 grams in 1 Liter distilled water. Adjust the pH to 7.2 Sterilize by autoclaving for 15 minutes


Ingredients:

  • Casein Peptone: 10 g/L
  • Sodium Chloride: 5 g/L
  • Yeast Extract: 5 g/L
  • Agar: 15 g/L



Research or further manufacturing use only, not for food or drug use.


  • Appearance: Powder
  • pH: 6.8 - 7.2
  • Solubility: Clear Solution

PR1MA Yeast Nitrogen Base without Amino Acids

Solubility: Water
Storage Temperature: Room Temperature


For classifying yeasts based on amino acid and carbohydrates requirements. Contains all essential vitamins and inorganic salts necessary for the cultivaiton of yeasts. Excludes histidine methionine, tryptophan and carbon source. Also contains <1 mg/L each of vitamins and trace inorganic salts.


Research or further manufacturing use only, not for food or drug use.


  • DCM Appearance: Free flowing powder, Homogeneous, Off white to cream
  • PPM Appearance: Pale to light yellow (10X Solution)
  • pH (0.67% Solution @25 oC): 5.2 - 5.6
  • Microorganism Koleckera Apiculata ATCC 9774 (With Additives): Good to Excellent
  • Microorganism Saccharomyces Cerevisiae ATCC 9080 (With Additives): Good to Excellent
  • Microorganism Saccharomyces Cerevisiae ATCC 9763 (With Additives): Good to Excellent
  • Microorganisms Candida Albicans ATCC 10231 (With Additives): Good to Excellent

PR1MA Lysozyme from Chicken Egg White, Salt Free

CAS Number: 12650-88-3

Solubility: Water

Storage Temperature: 2-8°C
 

Purified from chicken egg white, 2x crystallized, dialyzed and supplied as a salt free lyophilized powder.

Dry powder stable for 3 - 5 years when stored dry at 2°C to 8°C.

Stock solution is stable up to 3 days at 4°C and up to 1 year at -20°C.

Research or further manufacturing use only, not for food or drug use.
 

  • Appearance: 2X Crystallized; dialyzed, lyophilized powder
  • A 280@ mg/ml: Report
  • Protein: Report %
  • U/mg dw (Unit Per mg Dry Weight):  8000
  • Raw Material Species: Chicken Egg Whites
  • Country of Origin: Report

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