Molecular Biology Reagents/Chemicals

MIDSCI™ gives you ultra-pure, cost-competitive molecular biology reagents and lab chemicals for life science research; often with significant cost savings over Sigma Aldrich and other lab reagent providers.

PR1MA™ Chemicals can help with a wide variety of research-based requirements.
 
Use our glycerol for polyacrylamide gel electrophoresis. Eliminate Agrobacterium after inoculation with the carbenicillin we carry. Or detect β-Galactosidase activity with X-GAL which, when combined with the IPTG we stock, can be used to detect lac gene expression in cloning applications.
 
We also carry Luria Broth (Lennox), for the propagation and maintenance of E. coli; DTT, in order to maintain –SH groups in a reduced state; and high gel strength agar for tissue culture and microbiological work.

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PR1MA  Blasticidin S Hydrochloride 10 mg/mL Solution

CAS Number: 3513-03-9
Molecular Weight: 458.9
Chemical Formula: C17H26N8O5  HCl
Solubility: Water
Storage Temperature: -20°C

 
A peptidyl nucleoside antibiotic isolated from the culture broth of Streptomyces griseogenes, inhibits protein synthesis in both prokaryotes and eukaryates.


Supplied as a 10mg/ml sterile solution in HEPES buffer.
Research or further manufacturing use only, not for food or drug use.
 
  • Appearance: Clear Solution
  • Concentration: 10mg/ml sterile solution
  • Description: Colorless solution in HEPES buffer
  • pH: 7.2 - 7.5
  • Purity:  98.0 %

PR1MA Calcium Chloride Anhydrous

CAS Number: 10043-52-4
Molecular Weight: 110.98
Chemical Formula: CaCl2
Solubility: Water
Storage Temperature: Room Temperature


Research or further manufacturing use only, not for food or drug use.
 

  • Appearance: White crystalline powder
  • Assay (Titration):  96.0 %
  • Titrable Base:  0.006 meq/g

PR1MA  Agarose, Low EEO

CAS Number: 9012-36-6
Solubility: Water
Storage Temperature: Room Temperature


Molecular Biology Grade. High purity for preparative and analytical electrophoresis of nucleic acids >1000 bp.


Gelling Temperature: 34.5 -37.5°C
Sulfate: 0.15
EEO: 0.09- 0.13
DNA Binding: None Detected
DNA and RNase: None Detected


Research or further manufacturing use only, not for food or drug use.
 

  • Appearance: Powder
  • DNase/ RNase: Not Detected
  • EEO: 0.09 - 0.13
  • Gelling Point: 34.5 - 37.5 °C
  • Gel Strength (1.0%):  1200 g/cm²
  • Moisture Content:  10.0 %
  • Sulfate:  0.15 %

PR1MA Bromophenol Blue [3',3",5',5"-Tetrabromo-phenolsulfonephthalein]

Common Name: 3',3",5',5"-Tetrabromo-phenolsulfonephthalein
CAS Number: 115-39-9
Molecular Weight: 669.96
Chemical Formula: C19H10Br4O5S
Solubility: Methanol, ethanol
Storage Temperature: Room Temperature


Research or further manufacturing use only, not for food or drug use.


  • Appearance: Pink to Red Crystalline Powder
  • Clarity of Solution: Pass
  • Visual Transition Interval (pH 3.0 - 4.6): Pass

IBI Ethanol (Anhydrous Alcohol)

 

Introducing our premium-grade Ethanol, also known as Anhydrous Alcohol – the versatile solution for a myriad of applications. Sourced and refined with meticulous care, our Ethanol stands as a hallmark of purity and quality, ready to meet your diverse needs.

 

Our Ethanol is widely used for precipitating nucleic acids. The nucleic precipitate, which is formed in the presence of moderate concentrations of monovalent cations, is recovered by centrifugation and re-dissolved in an appropriate buffer at the desired concentration.

 

200 Proof Ethanol is denatured with methyl alcohol. It is also known as ethyl alcohol, alcohol anhydrous, denatured alcohol.

 

Specifications

 

CAS#: 64-17-5

200 Proof Ethanol denatured with 5% methanol

Used for precipitating nucleic acids

Specific Gravity: 0.7964 Max.
Formula Weight: 46.07
Molecular Formula: C2H5OH
Ethanol: 95%
Methanol: 5%
Moisture (KF): 1% Max.
Identification (IR): Pass


Molecular Biology Specifications

DNase assay: None Detected
RNase assay: None Detected

 

This product cannot be shipped to a personal residence

 

 

IBI Cell Culture Grade, Pyrogen/Endotoxin Free Water is prepared for nuclear transfer, transfection, transformation, cell and tissue culture,
LAL testing, and all other aqueous solution preparations that require stringent quality control.


IBI Cell Culture Grade Water is tested for use in critical assays and applications. The water is endotoxin-free (to the maximum detection
limit of <0.005EU/ml by turbidimetric assay). The purification process of our Cell Culture Grade Water includes distillation, continuous
deionization, reverse osmosis, 0.1µm filtration, followed by steam sterilization in an autoclave.


IB42010

Cell Culture Grade Water, Pyrogen and Endotoxin Free, 1L

IB42011

Cell Culture Grade Water, Pyrogen and Endotoxin Free, 6 x 1L

IB42012

Cell Culture Grade Water, Pyrogen and Endotoxin Free, 12 x 1L

IB42020

Cell Culture Grade Water, Pyrogen and Endotoxin Free, 2L

IB42021

Cell Culture Grade Water, Pyrogen and Endotoxin Free, 6 x 2L

IB42030

Cell Culture Grade Water, Pyrogen and Endotoxin Free, 10L

Item#:
ASMOLWATER

PR1MA  Rifampicin

CAS Number: 13292-46-1
Molecular Weight: 823.0
Chemical Formula: C43H58N4O12
Solubility: Dilute acid solutions
Storage Temperature: 2-8°C


Interferes with synthesis of nucleic acids by inhibiting DNA dependent RNA polymerase. Active against gram positive bacteria. Less active against gram negative bacteria. Plant cell culture tested.


Research or further manufacturing use only, not for food or drug use.


  • Appearance: Brownish-Red Crystalline Powder
  • Crystallinity: Pass Test
  • Heavy Metals :  20 ppm
  • Identification: Pass Test
  • Loss on Drying:  1.0 %
  • pH: 4.5 - 6.5
  • Purity: 97.0 - 102.0 %
  • Rifampicine Quinone:  1.5 %
  • Solubility: Pass
  • Sulfated Ash:  0.1 %

PR1MA  Hygromycin B Liquid

CAS Number: 31282-04-9
Molecular Weight: 527.53
Chemical Formula: C20H37N3O13
Solubility: Water
Storage Temperature: 2-8°C

 
Aminoglycoside antibiotic inhibits growth of prokaryotic (bacteria), eukaryotic microorganism (yeasts) and mammalian cells. Particularly useful as a selective agent for the incorporation of the APH 4 gene in plant tissue. Inhibits peptide chain elongation by preventing elongation-factor EF-2 dependent translocation. Supplied as 1 million units in solution per vial. 1 Million Units of Hygromycin

 
Research or further manufacturing use only, not for food or drug use.
 
  • Appearance: Light amber solution
  • Purity:  80 %
  • Potency : Report U/mg
  • Concentration: Report (500 mg Hygromycin B/ml) units/ml
  • Density: Report mg/ml

 

PR1MA LB Agar, Low Salt Formula, Granulated [Lennox L Agar]

Common Name: Lennox L Agar
CAS Number: 91079-40-2, 8013-01-2, 7647-14-5, 9002-18-0
Solubility: Water with Heat
Storage Temperature: Room Temperature


For propagation and maintenance of E. Coli. Dissolve 32 grams in 1 Liter distilled water and adjust the pH to 7.2. Sterilize by autoclaving for 15 minutes.


Ingredients:

  • Casein Peptone: 10 g/L
  • Sodium Chloride: 5 g/L
  • Yeast Extract: 5 g/L
  • Agar: 12 g/L


Research or further manufacturing use only, not for food or drug use.


  • DCM Appearance: Homogeneous Free Flowing ,Light Beige
  • PPM Appearance: Clear, no to light ppt, light yellow
  • pH (2.0% Solution @ 25 oC): 6.8 - 7.2
  • Microorganism (E. coli ATCC 23724): Good to Excellent
  • Microorganism (E. coli ATCC 33694): Good to Excellent
  • Microorganism (E. coli ATCC 33849): Fair to Excellent
  • Microorganism (E. coli ATCC 39403): Good to Excellent
  • Microorganism (E. coli ATCC 47014): Good to Excellent
  • Microorganism (E. coli ATCC 53868): Good to Excellent
  • Sodium Chloride: 5 g/l
  • Yeast Extract: 5 g/L
  • Agar: 12 g/l
  • Casein Peptone: 10 g/L

PR1MA PBS [Phosphate Buffered Saline], 10X Powder Concentrate, White Granular Powder

CAS Number: 7778-77-0; 7447-40-7; 7558-79-4; 7647-14-5
Storage Temperature: Room Temperature


Research or further manufacturing use only, not for food or drug use.


  • Appearance: White Uniform Powder (No Granular)
  • Chloride: 0.136 - 0.144 moles/liter
  • Conductivity @ 25 oC: 14000 - 17800 µomhos/cm
  • DNase, RNase, protease: Not Detected
  • pH (10X Solution): 6.7 - 6.9
  • pH (1 X Solution): 7.3 - 7.5
  • Solubility: Pass Test (9.89%)
  • Seal Integrity Testing: Pass Test

PR1MA  Sodium Phosphate Dibasic, Anhydrous, ACS Grade

CAS Number: 7558-79-4
Molecular Weight: 142.0
Chemical Formula: Na2HPO4
Solubility: Water
Storage Temperature: Room Temperature


Research or further manufacturing use only, not for food or drug use.


  • Appearance: White crystals
  • Assay:  99.0 %
  • Chloride:  0.002 %
  • Heavy Metals :  10 ppm
  • Insoluble Matter:  0.01 %
  • Iron:  0.002 %
  • Loss on Drying:  0.2 %
  • pH (5% Solution): 8.7 - 9.3
  • Sulfate:  0.005 %
  • Yeast and Mold Count:  100 CFU/g
  • Aerobic Plate Count:  1000 CFU/g

PR1MA LB Broth, Granulated [Lennox LB Broth]

Common Name: Lennox L Broth
Solubility: Water
Storage Temperature: Room Temperature


For propagation and maintenance of E. Coli. Low Salt Formula. Molecular Biology Grade.


Dissolve 20 grams in 1 liter distilled water. Then adjust the pH to 7.2. Heat and frequently agitate to completely dissolve. Material is specially granulated to reduce dust and increase solubility rate. Sterilize by autoclaving for 15 minutes.


Ingredients:

  • Tryptone:10 g/L
  • Sodium Chloride: 5 g/L
  • Yeast Extract: 5 g/L



Research or further manufacturing use only, not for food or drug use.


  • Appearance: Light beige color, average particle size between 1 to 2mm
  • DCM Appearance: Homogeneous, free flowing light beige
  • PPM Appearance: Clear, no to light ppt, light yellow
  • Appearance of Solution: Prepared medium is yellow to gold and clear to moderately hazy
  • pH (2.0% Solution @ 25 oC): 6.8 - 7.2
  • Microorganism (E. coli ATCC 23724): Good to Excellent
  • Microorganism (E. coli ATCC 33694): Good to Excellent
  • Microorganism (E. coli ATCC 33849): Fair to Excellent
  • Microorganism (E. coli ATCC 39403): Good to Excellent
  • Microorganism (E. coli ATCC 47014): Good to Excellent
  • Microorganism (E. coli ATCC 53868): Good to Excellent
  • Casein Peptone: 10 g/L
  • Sodium Chloride: 5 g/L
  • Yeast Extract: 5 g/L

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