Molecular Biology Reagents/Chemicals

MIDSCI™ gives you ultra-pure, cost-competitive molecular biology reagents and lab chemicals for life science research; often with significant cost savings over Sigma Aldrich and other lab reagent providers.

PR1MA™ Chemicals can help with a wide variety of research-based requirements.
 
Use our glycerol for polyacrylamide gel electrophoresis. Eliminate Agrobacterium after inoculation with the carbenicillin we carry. Or detect β-Galactosidase activity with X-GAL which, when combined with the IPTG we stock, can be used to detect lac gene expression in cloning applications.
 
We also carry Luria Broth (Lennox), for the propagation and maintenance of E. coli; DTT, in order to maintain –SH groups in a reduced state; and high gel strength agar for tissue culture and microbiological work.

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PR1MA Agar, Granulated, High Gel Strength

CAS Number: 9002-18-0
Solubility: Water with Heat
Storage Temperature: Room Temperature


Purified agar with excellent properties for tissue culture and microbiological work.

  • To prepare media, add 15 grams to 1 liter of water.


Research or further manufacturing use only, not for food or drug use.


  • Appearance: White Cream Color, Average Particle Size Between 1-2 mm
  • Granulometry (ASTM) 60 Mesh Sieve Size: Report %
  • Ash:  6.5 %
  • E.Coli: Negative
  • Gel Point (1.5% Gel): 33 - 38°C
  • Gel Strength (1.5% Gel): 600 - 1100 g/cm2
  • Melting Point: 88 - 96°C
  • Moisture :  15 %
  • Molds and Yeasts: < 100 cfu/g
  • pH: 6.0 - 8.0
  • Total Plate Count: < 1000 cfu/g
  • Viscosity (1.5 Solution @ 60 oC):  30 cps
  • Water Absorption: ≤ 75 cc

Radiance ECL Chemiluminescent Substrate

 

Chemiluminescent HRP substrate for chemiluminescent Western blots

Radiance ECL is an HRP substrate with long-lasting signal and sensitivity down to low picograms. Use up to 10 times less antibody than other substrates used in life science.

 

Size: 500 mL

 

Features

  • Performance — compare performance to SuperSignal West Pico and Amersham ECL
  • Sensitivity — high sensitivity in low picograms
  • Duration — Signal duration 6-8 hours
  • Chemiluminescent Western Blotting
  • The most common method for Western blot detection is chemiluminescence. Detection by chemiluminescence is relatively sensitive and works well for detecting individual or low abundance proteins.   


Item#:
ASRADIANCEECL

Radiance Plus Chemiluminescent Substrate

 

The most sensitive HRP substrate available for chemiluminescent Western blotting.

With attomole sensitivity and a long-lasting signal, Radiance Plus allows you to detect bands not visualized with other substrates. High signal-to-noise and a large dynamic range make it ideal for quantifying low-intensity bands.

 

Size: 150 mL

 

Features

  • Low-abundance protein detection
  • Compare performance to competitor products, like SuperSignal West Femto and Amersham ECL Select
  • Sensitivity in low femtograms
  • Signal duration 6-8 hours   

Item#:
ASRADIANCEPLUS

Radiance Q Chemiluminescent Substrate

 

Radiance Q chemiluminescent substrate is specially formulated for use with Azure Imaging Systems, or other CCD imagers. This novel horseradish peroxidase (HRP) substrate provides a strong, long-lasting signal, the broadest useful linear range, and high sensitivity for the most quantitative chemiluminescent Western assays.

 

Size: 150 mL

 

Features

Increase the sensitivity and quantitative accuracy of your chemiluminescent Western blots. Specially developed for CCD imaging, Radiance Q produces a strong, long-lasting signal with extremely low background, perfect for detecting low abundance proteins. Because the substrate is not depleted at high protein loads, Radiance provides the largest dynamic range of any chemiluminescent substrate, improving quantitative accuracy.

  • Extended dynamic range for detecting low and high abundance proteins within the same blot
  • Compare performance to SuperSignal West Dura and Amersham ECL Prime
  • Sensitivity in mid femtograms
  • Signal duration 10-24 hours   

 
 


Item#:
ASRADIANCEQ

PR1MA Agarose 500mg Tablets

CAS Number: 9012-36-6
Solubility: Water
Storage Temperature: Room Temperature


Save time and labor preparing agarose gels for electrophoresis of nucleic acids. Just place the required number of tablets into a standard buffer, then mix and heat until completely dissolved.


Tablet Weight Range: 485-515 mg
Gel Strength, 1.5%: >1200 g/cm2
Gelling Range: 36-39°C
Melting Temp: 87-89°C
EEO: <0.10
Sulfate: <0.15
DNase, RNase: None Detected


Research or further manufacturing use only, not for food or drug use.
 

  • Appearance: White tablets
  • Gelling Range (1.5% Water): 36 - 39 oC
  • pH (3% water, 25°C): 5.7 - 7.7
  • Tablet Weight: 485 - 515 mg

Electrophoresis Buffers
QuickSilver - TAE (Tris-acetate-EDTA)

TAE is ideal for separation of large DNA fragments in agarose gels and is used as running buffer and in gel preparation.

TAE is the buffer of choice when recovering DNA from gels and when downstream processing involves enzymatic reactions. Final concentration of a 1X working solution is 40mM Tris, 20mM acetic acid, 1mM EDTA.

QS1000 QuickSilver TAE Buffer, 1X, 100 packs

 

QuickSilver - TBE (Tris-borate-EDTA)

TBE is used in gel preparation and as a running buffer for electrophoresis of nucleic acids. TBE works best for resolution of smaller fragments (<2kb). Its higher buffering capacity makes it useful for longer runs. The final concentration of a 1X working solution is 89mM Tris, 89mM boric acid, 2mM EDTA.

QS1001 QuickSilver TBE Buffer, 1X, 50 packs

 

QuickSilver - Fast Running Buffer

QuickSilver Fast Running Buffer is a proprietary blend formulated for agarose gel electrophoresis at high voltage (300-350V). Its strong buffering capacity minimizes heat generation all allows for extremely fast separation of fragments smaller than 5kb. A typical 30-minute run can be performed in just 10 minutes using this buffer.

QS1002 QuickSilver Fast Running Buffer, 1X, 100 packs
 
 

Protein Electrophoresis Buffers

QuickSilver™ TGS (Tris-Glycine-SDS)

Tris-Glycine-SDS is one of the most common running buffers used for SDS-PAGE. A 1X solution contains 25mM Tris, 192mM Glycine and 0.1% SDS.

QS1100 QuickSilver Tris Glycine-SDS Buffer, 1X, 50 packs


QuickSilver™ Tris-MES-SDS

Small to medium molecular weight proteins can be resolved on SDS-PAGE gels using Tris-MES-SDS as a running buffer. Proteins resolve faster in gels using Tris-MES-SDS as a running buffer than those using Tris-MOPS-SDS. A 1X solution has a final concentration of 50mM Tris, 50mM MES, 0.1% SDS and 1mM EDTA.

QS1101 QuickSilver Tris-MES-SDS Buffer, 1X, 50 packs


QuickSilver™ Tris-MOPS-SDS

Tris-MOPS-SDS is ideal for use as a running buffer when resolving medium to large proteins with SDS-PAGE. Gels run with Tris-MOPS-SDS buffer run slower than those using Tris-MES-SDS buffer. The final concentration of a 1X working solution is 50mM Tris, 50mM MOPS, 0.1% SDS and 1mM EDTA.

QS1102 QuickSilver Tris-MOPS-SDS, 1X, 50 packs



Western Blotting, ELISA, Cell Culture, Chromatography

QuickSilver™ PBS and PBST

Because its osmolality and ion concentration closely mimic that of the human body, PBS is commonly used for cell culture applications, as well as HPLC and MS. PBST is a wash buffer and diluent for ELISA. The 1X PBS solution contains 137mN NaCl, 2.7mM KCl and 10mM phosphate. PBST is supplemented with 0.05% Tween-20.

QS1200 QuickSilver PBS Buffer, 1X, 100 packs

QS1201 QuickSilver PBST Buffer, 1X, 100 packs


QuickSilver™ TBS and TBST

TBS and TBST are used to maintain pH within a very narrow range for a variety of applications including western blotting and immunoassays. The final concentration of a 1X working solution is 137mM NaCl, 2.7mM KCl, 50mM Tris. In addition, 1X TBST contains 0.1% Tween-20.

QS1202 QuickSilver TBS buffer, 1X, 100 packs

QS1203 QuickSilver TBST buffer, 1X, 100 packs


Item#:
ASQUICKSILVER

PR1MA Agarose 3:1, Molecular Biology Grade

CAS Number: 9012-36-6
Solubility: Water
Storage Temperature: Room Temperature


Molecular Biology Grade. For DNA/RNA fragments 150-1500bp. Exceptional gel strength. DNase/RNase free.


Gel Strength, 4%: 
≥ 1400 g/cm2
Gelling Temperature (4%): 32-28°C
Melting Temperature (4%): 90°C
Sulfate: <0.15%
EEO: <0.13


Research or further manufacturing use only, not for food or drug use.

 

  • Appearance: White to Off White Powder
  • EEO:  0.13 -Mr
  • Gelling Temperature: 32.5 - 38.0 oC
  • Gel Strength :  1400 g/cm2
  • Melting Temperature:  90.0 oC
  • Moisture Content:  10.0 %
  • Sulfate:  0.15 %

PR1MA  TRIS Hydrochloride [Tris(hydroxymethyl) aminomethane HCl]

Common Name: Tris(hydroxymethyl) aminomethane hydrochloride
CAS Number: 1185-53-1
Molecular Weight: 157.6
Chemical Formula: C4H11NO3  HCl
Solubility: Water
Storage Temperature: Room Temperature


Highly purified for use in molecular biology applications.


Research or further manufacturing use only, not for food or drug use.


  • Appearance: White crystalline
  • Assay: 99.0 - 101.0 %
  • pH (1% aqueous solution): 4.2 - 5.0
  • Solubility (1M Solution in Water ) : Clear, colorless solution
  • UV Absorbance @260nm, 1.0 M in H2O:  0.06
  • UV Absorbance @280nm, 1.0 M in H2O:  0.05
  • Identification: Conforms
  • Heavy Metals :  5 ppm
  • Grade: USP

PR1MA Nystatin

CAS Number: 1400-61-9
Molecular Weight: 926.1
Chemical Formula: C47H75NO17
Solubility: DMSO
Storage Temperature: 2-8°C


Polyene antifungal antibiotic produced by Streptomyces noursei.

Research or further manufacturing use only, not for food or drug use.

  • Appearance: Yellow to light tan powder
  • Crystallinity: Pass Test
  • Identification: Pass Test
  • Loss on Drying:  5.0 %
  • pH @25 °C: 6.0 - 8.0
  • Potency (Assay):  4400 U/mg
  • Suspendability: Pass Test
  • Residual Solvent (n-butanol):  1000 ppm
  • Residual Solvent (Methanol):  3000 ppm
  • Residual Solvent (Acetone):  5000 ppm
  • Residual Solvents: The following Class 1,2, or 3 Solvents may be present in this product. All comply with the limits prescribed in the current USP.

 

PR1MA  MES Monohydrate [2-(N-Morpholino)-ethanesulfonic acid]

Common Name: 2-(N-Morpholino)ethanesulfonic acid
CAS Number: 145224-94-8
Molecular Weight: 213.24
Chemical Formula: C6H13NO4 H 2O
Solubility: Water
Storage Temperature: Room Temperature


Buffer for use in plant tissue culture.


  • pKa (20°C): 6.15
  • pH range: 5.5 - 6.7


Research or further manufacturing use only, not for food or drug use.
 

  • Appearance: White crystalline powder
  • Assay (Titration):  99.0 %
  • Heavy Metals:  0.0010 %
  • Loss on Drying: 7.0 - 10.0 %
  • pH: 2.5 - 4.0
  • pKa @20 oC: 6.02 - 6.25
  • Solubility (10% solution in H2O): Clear
  • Sulfate:  0.01 %
  • UV Absorbance 250nm in 10% H2O:  0.05
  • Country of Origin : Report

PR1MA bis-Acrylamide, 2% Solution

CAS Number: 110-26-9
Storage Temperature: 2-8°C


A 2% (w/v) bis-Acrylamide solution.


Research or further manufacturing use only, not for food or drug use.


  • Appearance: Clear, colorless liquid
  • Conductivity of Solution: Report µmhos
  • Electrophoresis: Pass Test
  • pH: Report

PR1MA™ Potassium Acetate

CAS Number: 127-08-2
Molecular Weight: 98.14
Chemical Formula: C2H3O2K
Solubility: Water
Storage Temperature: Room Temperature


Research or further manufacturing use only, not for food or drug use.


  • Appearance: White crystalline powder
  • Calcium (Ca):  0.005 %
  • Chloride (Cl):  0.003 %
  • Heavy Metals :  5 ppm
  • Insolubles:  0.005 %
  • Iron:  5 ppm
  • Magnesium (Mg):  0.002 %
  • pH (5% in water): 6.5 - 9.0
  • Phosphate:  0.001 %
  • Purity:  99.0 %
  • Sodium (Na):  0.03 %
  • Sulfate:  0.002 %
  • Country of Origin: Report
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