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Molecular Biology Reagents/Chemicals
MIDSCI™ gives you ultra-pure, cost-competitive molecular biology reagents and lab chemicals for life science research; often with significant cost savings over Sigma Aldrich and other lab reagent providers.
PR1MA™ Chemicals can help with a wide variety of research-based requirements.
Use our glycerol for polyacrylamide gel electrophoresis. Eliminate Agrobacterium after inoculation with the carbenicillin we carry. Or detect β-Galactosidase activity with X-GAL which, when combined with the IPTG we stock, can be used to detect lac gene expression in cloning applications.
We also carry Luria Broth (Lennox), for the propagation and maintenance of E. coli; DTT, in order to maintain –SH groups in a reduced state; and high gel strength agar for tissue culture and microbiological work.
PR1MA™ Agar, Granulated, High Gel Strength
CAS Number: 9002-18-0
Solubility: Water with Heat
Storage Temp: Room Temperature
Purified agar with excellent properties for tissue culture and
microbiological work.
- To prepare media, add 15 grams to 1 liter of water.
Research or further manufacturing use only, not for food or drug use.
- Appearance: White Cream Color, Average Particle Size Between 1-2 mm
- Granulometry (ASTM) 60 Mesh Sieve Size: Report %
- Ash: ≤ 6.5 %
- E.Coli: Negative
- Gel Point (1.5% Gel): 33 - 38°C
- Gel Strength (1.5% Gel): 600 - 1100 g/cm2
- Melting Point: 88 - 96°C
- Moisture : ≤ 15 %
- Molds and Yeasts: < 100 cfu/g
- pH: 6.0 - 8.0
- Total Plate Count: < 1000 cfu/g
- Viscosity (1.5 Solution @ 60 oC): ≤ 30 cps
- Water Absorption: ≤ 75 cc
Proudly made in the USA! Click here for more made in America products!
Radiance ECL Chemiluminescent Substrate
Chemiluminescent HRP substrate for chemiluminescent Western blots
Radiance ECL is an HRP substrate with long-lasting signal and sensitivity down to low picograms. Use up to 10 times less antibody than other substrates used in life science.
Size: 500 mL
Features
- Performance — compare performance to SuperSignal West Pico and Amersham ECL
- Sensitivity — high sensitivity in low picograms
- Duration — Signal duration 6-8 hours
- Chemiluminescent Western Blotting
- The most common method for Western blot detection is chemiluminescence. Detection by chemiluminescence is relatively sensitive and works well for detecting individual or low abundance proteins.
Radiance Plus Chemiluminescent Substrate
The most sensitive HRP substrate available for chemiluminescent Western blotting.
With attomole sensitivity and a long-lasting signal, Radiance Plus allows you to detect bands not visualized with other substrates. High signal-to-noise and a large dynamic range make it ideal for quantifying low-intensity bands.
Size: 150 mL
Features
- Low-abundance protein detection
- Compare performance to competitor products, like SuperSignal West Femto and Amersham ECL Select
- Sensitivity in low femtograms
- Signal duration 6-8 hours
Radiance Q Chemiluminescent Substrate
Radiance Q chemiluminescent substrate is specially formulated for use with Azure Imaging Systems, or other CCD imagers. This novel horseradish peroxidase (HRP) substrate provides a strong, long-lasting signal, the broadest useful linear range, and high sensitivity for the most quantitative chemiluminescent Western assays.
Size: 150 mL
Features
Increase the sensitivity and quantitative accuracy of your chemiluminescent Western blots. Specially developed for CCD imaging, Radiance Q produces a strong, long-lasting signal with extremely low background, perfect for detecting low abundance proteins. Because the substrate is not depleted at high protein loads, Radiance provides the largest dynamic range of any chemiluminescent substrate, improving quantitative accuracy.
- Extended dynamic range for detecting low and high abundance proteins within the same blot
- Compare performance to SuperSignal West Dura and Amersham ECL Prime
- Sensitivity in mid femtograms
- Signal duration 10-24 hours
Vion Ultrapure ASTM Type I Water
Our ultrapure, ASTM Type I, water meets the strictest standards defined by the American Society for Testing and Materials (ASTM). It is ideal for a wide range of ultra-sensitive laboratory uses include gas chromatography, HPLC, mass spectrometry, atomic absorption spectroscopy, and trace analysis.
Specifications
Resistivity | ≥ 18 MΩ |
Conductivity | ≤ 0.056 uS / cm |
Total Organic Carbon (TOC) | ≤ 50 ppb |
Silicate (as SiO2) | ≤ 3 ppb |
Chloride (Cl) | ≤ 1 ppb |
Sodium (Na) | ≤ 1 ppb |
Endotoxin | ≤ 0.03 EU / mL |
Heterotrophic BacteriaCount (HBC) | ≤ 10 CFU / L |
Proudly made in the USA! Click here for more made in America products!
Vion ACS Reagent Grade Water
Vion ACS Reagent grade water is guaranteed to meet the purity standards set by the American Chemical Society (ACS). It is a high-quality, highly purified water with minimal contaminants, and is ideal for use in laboratory settings for sensitive chemical analyses, like liquid chromatography and UV-Visible spectrophotometry, and reactions where extreme purity is required.
Specifications
Conductivity | ≤ 2.0 uS / cm |
Silicate (as SiO2) | ≤ 0.01 ppm |
Heavy Metals (as Pb) | ≤ 0.01 ppm |
Substances Reducing Permanganate | Passes Test |
Chloride (Cl) | ≤ 0.4 ppm |
Nitrate (NO3) | ≤ 0.4 ppm |
Phosphate (PO4) | ≤ 1 ppm |
Sulfate (SO4) | ≤ 1 ppm |
UV A300 nm | ≤ 0.005 |
UV A254 nm | ≤ 0.005 |
UV A210 nm | ≤ 0.010 |
UV A200 nm | ≤ 0.010 |
LC Absorbance | Passes Test |
LC Gradient Elution | Passes Test |
Proudly made in the USA! Click here for more made in America products!
PR1MA™ Agarose 500 mg Tablets
CAS Number: 9012-36-6
Solubility: Water
Storage Temp: Room Temperature
Save time and labor preparing agarose gels for electrophoresis of nucleic acids. Just place the required number of tablets into a standard buffer, then mix and heat until completely dissolved.
Tablet Weight Range: 485-515 mg
Gel Strength, 1.5%: >1200 g/cm2
Gelling Range: 36-39°C
Melting Temp: 87-89°C
EEO: <0.10
Sulfate: <0.15
DNase, RNase: None Detected
Research or further manufacturing use only, not for food or drug use.
- Appearance: White tablets
- Gelling Range (1.5% Water): 36 - 39 oC
- pH (3% water, 25°C): 5.7 - 7.7
- Tablet Weight: 485 - 515 mg
Proudly made in the USA! Click here for more made in America products!
Electrophoresis Buffers
QuickSilver - TAE (Tris-acetate-EDTA)
TAE is ideal for separation of large DNA fragments in agarose gels and is used as running buffer and in gel preparation.
TAE is the buffer of choice when recovering DNA from gels and when downstream processing involves enzymatic reactions. Final concentration of a 1X working solution is 40mM Tris, 20mM acetic acid, 1mM EDTA.
QS1000 QuickSilver TAE Buffer, 1X, 100 packs
QuickSilver - TBE (Tris-borate-EDTA)
TBE is used in gel preparation and as a running buffer for electrophoresis of nucleic acids. TBE works best for resolution of smaller fragments (<2kb). Its higher buffering capacity makes it useful for longer runs. The final concentration of a 1X working solution is 89mM Tris, 89mM boric acid, 2mM EDTA.
QS1001 QuickSilver TBE Buffer, 1X, 50 packs
QuickSilver - Fast Running Buffer
QuickSilver Fast Running Buffer is a proprietary blend formulated for agarose gel electrophoresis at high voltage (300-350V). Its strong buffering capacity minimizes heat generation all allows for extremely fast separation of fragments smaller than 5kb. A typical 30-minute run can be performed in just 10 minutes using this buffer.
QS1002 QuickSilver Fast Running Buffer, 1X, 100 packs
Protein Electrophoresis Buffers
QuickSilver™ TGS (Tris-Glycine-SDS)
Tris-Glycine-SDS is one of the most common running buffers used for SDS-PAGE. A 1X solution contains 25mM Tris, 192mM Glycine and 0.1% SDS.
QS1100 QuickSilver Tris Glycine-SDS Buffer, 1X, 50 packs
QuickSilver™ Tris-MES-SDS
Small to medium molecular weight proteins can be resolved on SDS-PAGE gels using Tris-MES-SDS as a running buffer. Proteins resolve faster in gels using Tris-MES-SDS as a running buffer than those using Tris-MOPS-SDS. A 1X solution has a final concentration of 50mM Tris, 50mM MES, 0.1% SDS and 1mM EDTA.
QS1101 QuickSilver Tris-MES-SDS Buffer, 1X, 50 packs
QuickSilver™ Tris-MOPS-SDS
Tris-MOPS-SDS is ideal for use as a running buffer when resolving medium to large proteins with SDS-PAGE. Gels run with Tris-MOPS-SDS buffer run slower than those using Tris-MES-SDS buffer. The final concentration of a 1X working solution is 50mM Tris, 50mM MOPS, 0.1% SDS and 1mM EDTA.
QS1102 QuickSilver Tris-MOPS-SDS, 1X, 50 packs
Western Blotting, ELISA, Cell Culture, Chromatography
QuickSilver™ PBS and PBST
Because its osmolality and ion concentration closely mimic that of the human body, PBS is commonly used for cell culture applications, as well as HPLC and MS. PBST is a wash buffer and diluent for ELISA. The 1X PBS solution contains 137mN NaCl, 2.7mM KCl and 10mM phosphate. PBST is supplemented with 0.05% Tween-20.
QS1200 QuickSilver PBS Buffer, 1X, 100 packs
QS1201 QuickSilver PBST Buffer, 1X, 100 packs
QuickSilver™ TBS and TBST
TBS and TBST are used to maintain pH within a very narrow range for a variety of applications including western blotting and immunoassays. The final concentration of a 1X working solution is 137mM NaCl, 2.7mM KCl, 50mM Tris. In addition, 1X TBST contains 0.1% Tween-20.
QS1202 QuickSilver TBS buffer, 1X, 100 packs
QS1203 QuickSilver TBST buffer, 1X, 100 packs
Citric Acid, Monohydrate
- Appearance (Color): Colorless or White
- Appearance (Form): Crystals
- Purity: 99.0 - 102.0%
- Identification (FTIR): Conforms to Structure
- Water: 7.5 - 8.8%
- Lead: ≤ 3 ppm
- Iron: ≤ 3 ppm
- Oxalate: To Pass Test
- Sulfate: ≤ 20 ppm
- Chloride: ≤ 10 ppm
- Readily Carbonizable Substances: Passes Test
- Insoluble Matter: ≤ 0.005%
- Residue After Ignition: ≤ 0.02%
- DNase: None Detected
- RNase: None Detected
*Custom sizes available upon request.
DOT Information: Non-regulated.
Sodium Bicarbonate
- Appearance: White Crystalline Powder
- Purity: 99.0 - 100.5%
- pH (5% Aqueous): 7.9 - 8.4
- Water: ≤ 0.25%
- Lead: ≤ 5 ppm
- Arsenic: ≤ 2 ppm
- Chloride: ≤ 150 ppm
- Sulfate: ≤ 150 ppm
- RNase: None Detected
- DNase: None Detected
*Custom sizes available upon request.
DOT Information: Non-regulated.
Tris Hydrochloride
- Appearance: White, Crystalline Powder
- Purity: ≥ 99.0%
- pH (1% Aqueous): 4.2 - 5.0
- Solubility (1M Aqueous): Clear and Colorless
- Identity (FTIR): Conforms to Structure
- Loss on Drying (105°C/4h): ≤ 0.5%
- Melting Point: 149ºC - 153ºC
- A260 (40% Aqueous): ≤ 0.06
- A280 (40% Aqueous): ≤ 0.05
- Lead: 5 ppm
- RNase: None Detected
- DNase: None Detected
*Custom sizes available upon request.
DOT Information: Non-regulated.
Guanidine Hydrochloride
- Appearance: White Crystalline Powder
- Purity: ≥ 99.0%
- Solubility (6M Aqueous): Clear and Colorless
- pH: 5.0 - 7.0
- Identity (FTIR): Conforms to Structure
- Water: ≤ 0.3%
- Heavy Metals (as Fe): ≤ 5 ppm
- A260 (6M Aqueous): ≤ 0.10
- A280 (6M Aqueous) ≤ 0.05
- RNase: None Detected
- DNase: None Detected
*Custom sizes available upon request.
DOT Information: Non-regulated.