PR1MA™ 2XYT Broth, Granulated

CAS Number: 91079-40-2; 8013-01-2; 7647-14-5
Solubility: Water
Storage Temperature: Room Temperature

Nutritionally enriched medium for the growth of recombinant strains of E. Coli and in the preparation and transformation of competent cells.


Material is specially granulated to reduce dust and increase solubility rate.


Dissolve 31g in one liter distilled water. Heat solution to boiling with continuous stirring. Sterilize by autoclaving at 121°C for 15 minutes. Final pH@ 25°C: 6.8-7.2.


Ingredients:

• Digest Peptone: 16 g/l
• Yeast Extract: 10 g/l
• Sodium Chloride: 5 g/l

Research or further manufacturing use only, not for food or drug use.

• Appearance: Cream to beige free flowing granulated
• DCM Appearance: Homogeneous free flowing, beige
• PPM Appearance: Clear,no to light ppt,yellow
• pH (3.1 % Solution @25 ^oC): 6.8 - 7.2
• Microorganism (E. coli ATCC 23724): Good to Excellent
• Microorganism (E. coli ATCC 33694): Good to Excellent
• Microorganism (E. coli ATCC 33849): Good to Excellent
• Microorganism (E. coli ATCC 39403): Good to Excellent
• Microorganism (E. coli ATCC 47014): Good to Excellent
• Microorganism (E. coli ATCC 53868): Good to Excellent

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PR1MA™ Casamino Acids [Casein acid hydrolysate]

Common Name: Casein acid hydrolysate
CAS Number: 91079-40-2
Solubility: Water
Storage Temperature: Room Temperature

Casein Acid Hydrolysate is a hydrochloric acid digest of casein that contains small amounts of cystine and Tryptophan. All vitamins have been eliminated by the acidification treatment. Provides a source of amino acids as nutrients for growing various organisms tolerant of high salt conditions.


Research or further manufacturing use only, not for food or drug use.

• Appearance: Homogeneous, Free Flowing Powder
• Color: Light Tan Yellow
• Amino Nitrogen: ≥ 4.0 %
• Moisture : ≤ 6.0 %
• pH: 6.0 - 7.5
• Protein: Report %
• Solution (2%): Clear Light Yellow
• Total Nitrogen: > 7.5 %
• Coliforms: < 10 cfu/g
• Standard Plate Count : < 5000 cfu/g
• Thermophile Count: Report cfu/g
• Source: Bovine

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PR1MA™  G-418 Disulfate

• Appearance: White Powder
• Absorbance @ 280nm (1mg/mL in H₂O): ≤ 0.015 nm
• Absorbance @ 570nm (100mg/mL in H₂O): ≤ 0.10 nm
• Ammonia: ≤ 1.0 %
• HPLC (Purity) : Report %
• Loss on Drying: ≤ 10.0 %
• pH: 5.0 - 6.0
• Potency (Dry basis): ≥ 700 µg/mg
• Purity (TLC-Spot): ≤ 3 Spots
• Solubility (100mg/ml in H₂O): Colorless Solution
• Specific Rotation: +104 - +121 °
• Non Animal Origin: The product does not come in contact with materials of animal origin.

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PR1MA™  Potassium Phosphate, Dibasic

CAS Number: 7758-11-4
Molecular Weight: 174.2
Chemical Formula: K₂HPO₄
Solubility: Water
Storage Temperature: Room Temperature

Research or further manufacturing use only, not for food or drug use.

• Appearance: White Powder
• Aerobic Plate Count:  ≤  1000 CFU/g
• Assay: ≥ 98.0 %
• Chloride (Cl): ≤ 0.003 %
• Heavy Metals : ≤ 5 ppm
• Insoluble Matter: ≤ 0.01 %
• Iron: ≤ 0.001 %
• Loss on Drying: ≤ 1.0 %
• Nitrogen Compound: ≤ 0.001 %
• pH (5% Solution): 8.5 - 9.6
• Sodium (Na): ≤ 0.05 %
• Sulfate: ≤ 0.005 %
• Total Coliforms: Negative
• Yeast and Mold Count: ≤ 100 CFU/g

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PR1MA™ Tryptone, Powder

CAS Number: 91079-40-2
Solubility: Water
Storage Temperature: Room Temperature

Pancreatic digest of casein used to supplement media in cultivation of anaerobic bacteria.


pH (1% solution): 7.3.


Ingredients:

• Molecular Biology GradeTotal Nitrogen (TN): ~12.7%
• Amino Nitrogen (AN): ~3.7%
• AN/TN Ratio: 0.26
• Sodium Chloride: ~0.4%
• Tryptophan: ~1.0

Research or further manufacturing use only, not for food or drug use.

• Appearance: Cream to beige free flowing powder
• Amino Nitrogen: ≥ 4.0 %
• Ash: ≤ 15 %
• Loss on Drying: ≤ 5.0 %
• Nitrites: Negative
• pH (2% water) @25°C: 6.5 - 7.5
• Total Nitrogen: ≥ 10 %
• Undigested Protein: Negative
• Broth Formulations (Growth Supporting Properties): Satisfactory
• Peptone Agar (Growth Supporting Properties): Satisfactory
• Proteoses: Positive
• Salmonella: Negative/25g
• Standard Plate Count : ≤ 1000 cfu/g
• Coliform: Negative
• E.Coli: Negative
• Molds and Yeasts: ≤ 100 cfu/g
• Raw Material Species: Bovine
• Enzyme Species: Porcine
• BSE/TSE Free: BSE/TSE Free
• Country of Origin : Report

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PR1MA™ Peptone, Granulated

CAS Number: 73049-73-7
Solubility: Water
Storage Temperature: Room Temperature

Mixtures of peptides and free amino acids from peptic digest of animal tissue.

• Amino Nitrogen (AN): 2.5-3.5%
• Loss on Drying: <6.0%

Research or further manufacturing use only, not for food or drug use.

• Appearance: Light Beige Granular Powder
• Amino Nitrogen Content: ≥ 3.4 %
• Appearance of Solution: Clear and yellow-beige
• Ash: ≤ 15.0 %
• Loss on Drying: ≤ 5.0 %
• pH (2.0% Solution @ 25 ^oC): 6.5 - 7.5
• Total Nitrogen Content: ≥ 10.0 %

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PR1MA™ CHAPS [3-[(3-Cholamidopropyl)-dimethylammonio]-1-propanesulfonate]

Common Name: 3-[(Cholamidopropyl) dimethylammonio]-1-propanesulfonate
CAS Number: 75621-03-3
Molecular Weight: 614.9
Chemical Formula: C₃₂H₅₈N₂SO₇
Solubility: Water
Storage Temperature: Room Temperature


Zwitterionic, non-denaturing detergent for solubilizing membrane proteins.

Research or further manufacturing use only, not for food or drug use.

• Appearance: White Powder
• Identification: Pass Test
• Moisture : ≤ 2.0 %
• Purity: ≥ 99 %
• RNase/DNase/Protease: None Detected
• Solubility (20%, water): Complete / Colorless
• TLC (silica gel: methanol/NH₄OH = 95/5): One Spot

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PR1MA™  Ticarcillin Disodium Salt

CAS Number: 4697-14-7
Molecular Weight: 428.39
Chemical Formula: C₁₅H₁₄N₂Na₂O ₆S₂
Solubility: Water
Storage Temperature: 2-8°C
 
 

Inhibits bacterial cell wall synthesis. Highly active against gram negative bacteria such as Agrobacterium strains. -Lactamase inhibitor. Hygroscopic.


Research or further manufacturing use only, not for food or drug use.
 
 

• Appearance: White to yellow crystalline powder
• Assay (Ticarcillin Content, dried basis): ≥ 80.0 %
• Assay (Ticarcillin, dried basis): ≥ 800 µg/mg
• Heavy Metals : ≤ 10 ppm
• Identification: Pass Test
• pH: 6.0 - 8.0
• Related Substances (Largest Impurity): ≤ 4.0 %
• Related Substances (Second Major Impurity) : ≤ 2.5 %
• Residual Solvents: Meets the requirements
• Specific Rotation: +172 - +187 °
• Sterility: Meet the requirements
• Water: ≤ 6.0 %
• Bacterial Endotoxins : ≤ 0.05 EU/mg
• Grade: Conforms to USP Specifications

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PR1MA™ Sodium Chloride

CAS Number: 7647-14-5
Molecular Weight: 58.4
Chemical Formula: NaCl
Solubility: Water
Storage Temperature: Room Temperature

Research or further manufacturing use only, not for food or drug use.

• Appearance: Fine White Granular Powder
• Acidity or Alkalinity: Pass Test
• Appearance of Solution: Clear, Colorless Solution
• Arsenic : ≤ 1 µg/g
• Assay: 99.0 - 100.5 %
• Barium: Pass Test
• Bromides: ≤ 0.010 %
• Ferrocyanide: Pass Test
• Heavy Metals : ≤ 5 ppm
• Identification (Chloride): Pass Test
• Identification (Sodium): Pass Test
• Loss on Drying: ≤ 0.5 %
• Magnesium & Alkaline Earth Metals:  0.01 %
• Iron(Fe): ≤ 2 µg/g
• Nitrites: ≤ 0.01
• Phosphates : ≤ 0.0025 %
• Sulfate: ≤ 0.020 %

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PR1MA™ Rapid Commassie Stain

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PR1MA™ Protein Gel-Loading Dye for SDS-PAGE

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Electrophoresis Buffers
QuickSilver - TAE (Tris-acetate-EDTA)

TAE is ideal for separation of large DNA fragments in agarose gels and is used as running buffer and in gel preparation.

TAE is the buffer of choice when recovering DNA from gels and when downstream processing involves enzymatic reactions. Final concentration of a 1X working solution is 40mM Tris, 20mM acetic acid, 1mM EDTA.

QS1000 QuickSilver TAE Buffer, 1X, 100 packs

QuickSilver - TBE (Tris-borate-EDTA)

TBE is used in gel preparation and as a running buffer for electrophoresis of nucleic acids. TBE works best for resolution of smaller fragments (<2kb). Its higher buffering capacity makes it useful for longer runs. The final concentration of a 1X working solution is 89mM Tris, 89mM boric acid, 2mM EDTA.

QS1001 QuickSilver TBE Buffer, 1X, 50 packs

QuickSilver - Fast Running Buffer

QuickSilver Fast Running Buffer is a proprietary blend formulated for agarose gel electrophoresis at high voltage (300-350V). Its strong buffering capacity minimizes heat generation all allows for extremely fast separation of fragments smaller than 5kb. A typical 30-minute run can be performed in just 10 minutes using this buffer.

QS1002 QuickSilver Fast Running Buffer, 1X, 100 packs
 
 

Protein Electrophoresis Buffers

QuickSilver™ TGS (Tris-Glycine-SDS)

Tris-Glycine-SDS is one of the most common running buffers used for SDS-PAGE. A 1X solution contains 25mM Tris, 192mM Glycine and 0.1% SDS.

QS1100 QuickSilver Tris Glycine-SDS Buffer, 1X, 50 packs

QuickSilver™ Tris-MES-SDS

Small to medium molecular weight proteins can be resolved on SDS-PAGE gels using Tris-MES-SDS as a running buffer. Proteins resolve faster in gels using Tris-MES-SDS as a running buffer than those using Tris-MOPS-SDS. A 1X solution has a final concentration of 50mM Tris, 50mM MES, 0.1% SDS and 1mM EDTA.

QS1101 QuickSilver Tris-MES-SDS Buffer, 1X, 50 packs

QuickSilver™ Tris-MOPS-SDS

Tris-MOPS-SDS is ideal for use as a running buffer when resolving medium to large proteins with SDS-PAGE. Gels run with Tris-MOPS-SDS buffer run slower than those using Tris-MES-SDS buffer. The final concentration of a 1X working solution is 50mM Tris, 50mM MOPS, 0.1% SDS and 1mM EDTA.

QS1102 QuickSilver Tris-MOPS-SDS, 1X, 50 packs

Western Blotting, ELISA, Cell Culture, Chromatography

QuickSilver™ PBS and PBST

Because its osmolality and ion concentration closely mimic that of the human body, PBS is commonly used for cell culture applications, as well as HPLC and MS. PBST is a wash buffer and diluent for ELISA. The 1X PBS solution contains 137mN NaCl, 2.7mM KCl and 10mM phosphate. PBST is supplemented with 0.05% Tween-20.

QS1200 QuickSilver PBS Buffer, 1X, 100 packs

QS1201 QuickSilver PBST Buffer, 1X, 100 packs

QuickSilver™ TBS and TBST

TBS and TBST are used to maintain pH within a very narrow range for a variety of applications including western blotting and immunoassays. The final concentration of a 1X working solution is 137mM NaCl, 2.7mM KCl, 50mM Tris. In addition, 1X TBST contains 0.1% Tween-20.

QS1202 QuickSilver TBS buffer, 1X, 100 packs

QS1203 QuickSilver TBST buffer, 1X, 100 packs