Molecular Biology Reagents/Chemicals

MIDSCI™ gives you ultra-pure, cost-competitive molecular biology reagents and lab chemicals for life science research; often with significant cost savings over Sigma Aldrich and other lab reagent providers.

PR1MA™ Chemicals can help with a wide variety of research-based requirements.
 
Use our glycerol for polyacrylamide gel electrophoresis. Eliminate Agrobacterium after inoculation with the carbenicillin we carry. Or detect β-Galactosidase activity with X-GAL which, when combined with the IPTG we stock, can be used to detect lac gene expression in cloning applications.
 
We also carry Luria Broth (Lennox), for the propagation and maintenance of E. coli; DTT, in order to maintain –SH groups in a reduced state; and high gel strength agar for tissue culture and microbiological work.

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PR1MA  Potassium Phosphate, Dibasic

CAS Number: 7758-11-4
Molecular Weight: 174.2
Chemical Formula: K2HPO4
Solubility: Water
Storage Temperature: Room Temperature


Research or further manufacturing use only, not for food or drug use.


  • Appearance: White Powder
  • Aerobic Plate Count:  ≤  1000 CFU/g
  • Assay:  98.0 %
  • Chloride (Cl):  0.003 %
  • Heavy Metals :  5 ppm
  • Insoluble Matter:  0.01 %
  • Iron:  0.001 %
  • Loss on Drying:  1.0 %
  • Nitrogen Compound:  0.001 %
  • pH (5% Solution): 8.5 - 9.6
  • Sodium (Na):  0.05 %
  • Sulfate:  0.005 %
  • Total Coliforms: Negative
  • Yeast and Mold Count:  100 CFU/g

PR1MA Tryptone, Powder

CAS Number: 91079-40-2
Solubility: Water
Storage Temperature: Room Temperature


Pancreatic digest of casein used to supplement media in cultivation of anaerobic bacteria.


pH (1% solution): 7.3.


Ingredients:

  • Molecular Biology GradeTotal Nitrogen (TN): ~12.7%
  • Amino Nitrogen (AN): ~3.7%
  • AN/TN Ratio: 0.26
  • Sodium Chloride: ~0.4%
  • Tryptophan: ~1.0


Research or further manufacturing use only, not for food or drug use.


  • Appearance: Cream to beige free flowing powder
  • Amino Nitrogen:  4.0 %
  • Ash:  15 %
  • Loss on Drying:  5.0 %
  • Nitrites: Negative
  • pH (2% water) @25°C: 6.5 - 7.5
  • Total Nitrogen:  10 %
  • Undigested Protein: Negative
  • Broth Formulations (Growth Supporting Properties): Satisfactory
  • Peptone Agar (Growth Supporting Properties): Satisfactory
  • Proteoses: Positive
  • Salmonella: Negative/25g
  • Standard Plate Count :  1000 cfu/g
  • Coliform: Negative
  • E.Coli: Negative
  • Molds and Yeasts:  100 cfu/g
  • Raw Material Species: Bovine
  • Enzyme Species: Porcine
  • BSE/TSE Free: BSE/TSE Free
  • Country of Origin : Report

PR1MA Peptone, Granulated

CAS Number: 73049-73-7
Solubility: Water
Storage Temperature: Room Temperature


Mixtures of peptides and free amino acids from peptic digest of animal tissue.

  • Amino Nitrogen (AN): 2.5-3.5%
  • Loss on Drying: <6.0%



Research or further manufacturing use only, not for food or drug use.


  • Appearance: Light Beige Granular Powder
  • Amino Nitrogen Content:  3.4 %
  • Appearance of Solution: Clear and yellow-beige
  • Ash:  15.0 %
  • Loss on Drying:  5.0 %
  • pH (2.0% Solution @ 25 oC): 6.5 - 7.5
  • Total Nitrogen Content:  10.0 %
[3-[(3-Cholamidopropyl)-dimethylammonio]-1-propanesulfonate]

PR1MA CHAPS [3-[(3-Cholamidopropyl)-dimethylammonio]-1-propanesulfonate]

Common Name: 3-[(Cholamidopropyl) dimethylammonio]-1-propanesulfonate
CAS Number: 75621-03-3
Molecular Weight: 614.9
Chemical Formula: C32H58N2SO7
Solubility: Water
Storage Temperature: Room Temperature


Zwitterionic, non-denaturing detergent for solubilizing membrane proteins.

Research or further manufacturing use only, not for food or drug use.

  • Appearance: White Powder
  • Identification: Pass Test
  • Moisture :  2.0 %
  • Purity:  99 %
  • RNase/DNase/Protease: None Detected
  • Solubility (20%, water): Complete / Colorless
  • TLC (silica gel: methanol/NH4OH = 95/5): One Spot

 

PR1MA  Ticarcillin Disodium Salt

CAS Number: 4697-14-7
Molecular Weight: 428.39
Chemical Formula: C15H14N2Na2O 6S2
Solubility: Water
Storage Temperature: 2-8°C
 
 

Inhibits bacterial cell wall synthesis. Highly active against gram negative bacteria such as Agrobacterium strains. -Lactamase inhibitor. Hygroscopic.


Research or further manufacturing use only, not for food or drug use.
 
 

  • Appearance: White to yellow crystalline powder
  • Assay (Ticarcillin Content, dried basis):  80.0 %
  • Assay (Ticarcillin, dried basis):  800 µg/mg
  • Heavy Metals :  10 ppm
  • Identification: Pass Test
  • pH: 6.0 - 8.0
  • Related Substances (Largest Impurity):  4.0 %
  • Related Substances (Second Major Impurity) :  2.5 %
  • Residual Solvents: Meets the requirements
  • Specific Rotation: +172 - +187 °
  • Sterility: Meet the requirements
  • Water:  6.0 %
  • Bacterial Endotoxins :  0.05 EU/mg
  • Grade: Conforms to USP Specifications

PR1MA Sodium Chloride

CAS Number: 7647-14-5
Molecular Weight: 58.4
Chemical Formula: NaCl
Solubility: Water
Storage Temperature: Room Temperature


Research or further manufacturing use only, not for food or drug use.


  • Appearance: Fine White Granular Powder
  • Acidity or Alkalinity: Pass Test
  • Appearance of Solution: Clear, Colorless Solution
  • Arsenic :  1 µg/g
  • Assay: 99.0 - 100.5 %
  • Barium: Pass Test
  • Bromides:  0.010 %
  • Ferrocyanide: Pass Test
  • Heavy Metals :  5 ppm
  • Identification (Chloride): Pass Test
  • Identification (Sodium): Pass Test
  • Loss on Drying:  0.5 %
  • Magnesium & Alkaline Earth Metals:  0.01 %
  • Iron(Fe):  2 µg/g
  • Nitrites:  0.01
  • Phosphates :  0.0025 %
  • Sulfate:  0.020 %
[Brain Heart Infusion Broth]

PR1MA™  BHI Broth [Brain Heart Infusion Broth]

Common Name: Brain Heart Infusion Broth
Solubility: Water
Storage Temperature: Room Temperature


Recommended for the cultivation of rare microorganisms. Adding 0.1% Agar will enhance the growth of anaerobic organisms. Store at room temperature and protect from moisture and light.


Ingredients:

  • Brain Heart Infusion from solids: 17.5 g/l
  • Pancreatic Digest of Gelatin: 10.0 g/l
  • Dextrose: 2.0 g/l
  • Sodium Chloride: 5.0 g/l
  • Disodium Phosphate: 2.5 g/l


Research or further manufacturing use only, not for food or drug use.


  • DCM Appearance: Homogeneous free flowing, beige
  • PPM Appearance: Light to Medium Amber/Clear
  • pH (3.7% H2O @25 oC): 7.2 - 7.6
  • Microorganism (Aspergillus niger ATCC 16404): Poor to Very Good
  • Microorganism (Candida albicans ATCC 10231): Good to Excellent
  • Microorganism (Enterococcus faecalis ATCC 29212): Good to Excellent
  • Microorganism (Escherichia Coli ATCC 25922): Very Good to Excellent
  • Microorganism (Neisseria meningitides ATCC 13090): Fair to Good
  • Microorganism -Pseudomonas Aeruginosa ATCC 27853: Good to Excellent
  • Microorganism (Saccharomyces cerevisiae ATCC 9763): Good to Excellent
  • Microorganism (Staphylcoccus Aureus ATCC 25923): Very Good to Excellent
  • Microorganism (Streptococcus pneumoniae ATCC 6305): Good to Excellent
  • Microorganism (Streptococcus pyogenes ATCC 19615): Good to Excellent
  • Raw Material Species: Bovine
  • Enzyme Species: Porcine

PR1MA Gelrite™

CAS Number: 71010-52-1
Solubility: Water
Storage Temperature: Room Temperature

Naturally derived gelling polymer that can be used in place of agar. Gelrite forms clear, rigid gels at approximately half the use level of agar in presence of soluble salts like Mg2+ and Ca2+.

Research or further manufacturing use only, not for food or drug use.

  • Appearance: Solid
  • Gel Strength: 400 - 700 g/cm2
  • Loss on Drying:  15.0 %
  • Particle Size 42 Mesh (355 µm):  95 %
  • Transmittance:  80.0 %

 

PR1MA Agar, Phyto

CAS Number: 9002-18-0
Solubility: Water
Storage Temperature: Room Temperature


Specially selected and tested agar for use in plant tissue culture. High gel strength.


Concentration of 5.0 g/l obtains a solid gel strength: (1600 g/cm2).


Research or further manufacturing use only, not for food or drug use.


  • Appearance: Pale Yellow Powder
  • Gelling Point: 34 - 36 °C
  • Gel Strength : 950 - 1050 g/cm2
  • Identification: Conforms
  • Loss on Drying:  18 %
  • Melting Point: 85 - 95 oC
  • Nephelos:  40 NTU
  • pH: 6.0 - 7.5
  • Total Ash:  3.5 %
  • Gelatin: Absent
  • Starch: Absent
  • Total Plate Count:  1000 cfu/g
[Casein acid hydrolysate]

PR1MA Casamino Acids [Casein acid hydrolysate]

Common Name: Casein acid hydrolysate
CAS Number: 91079-40-2
Solubility: Water
Storage Temperature: Room Temperature


Casein Acid Hydrolysate is a hydrochloric acid digest of casein that contains small amounts of cystine and Tryptophan. All vitamins have been eliminated by the acidification treatment. Provides a source of amino acids as nutrients for growing various organisms tolerant of high salt conditions.


Research or further manufacturing use only, not for food or drug use.


  • Appearance: Homogeneous, Free Flowing Powder
  • Color: Light Tan Yellow
  • Amino Nitrogen:  4.0 %
  • Moisture :  6.0 %
  • pH: 6.0 - 7.5
  • Protein: Report %
  • Solution (2%): Clear Light Yellow
  • Total Nitrogen: > 7.5 %
  • Coliforms: < 10 cfu/g
  • Standard Plate Count : < 5000 cfu/g
  • Thermophile Count: Report cfu/g
  • Source: Bovine

PR1MA Glycerol

CAS Number: 56-81-5
Molecular Weight: 92.1
Chemical Formula: C3H8O3
Solubility: Water
Storage Temperature: Room Temperature


Used in sample preparation and gel formation for polyacrylamide gel electrophoresis. Also used in casting gradient gels, as a protein stabilizer and storage buffer component.


Research or further manufacturing use only, not for food or drug use.


  • Appearance: Clear and Colorless Liquid
  • Chloride (Cl):  0.001 %
  • Chlorinated Compounds:  0.003 %
  • Color, APHA:  10.0
  • Fatty Acids & Esters (USP):  1.0 (ml 0.5 N NaOH) %
  • Glycerine :  99.7 %
  • Heavy Metals :  5.0 ppm
  • Identification C (GC): Pass
  • Limit of Diethylene Glycol & RC: Pass
  • Purity (USP): 99.0 - 101.0 %
  • Residue on Ignition:  0.01 %
  • Specific Gravity:  1.259
  • Water:  5.0 %
  • Date of Manufacture: Report


PR1MA Agar, High Gel Strength, Powder

CAS Number: 9002-18-0
Solubility: Water
Storage Temperature: Room Temperature


Purified high gel strength agar with excellent properties for tissue culture and microbiological work.


Molecular Biology Grade


Research or further manufacturing use only, not for food or drug use.


  • Appearance: White to Cream Powder
  • Ash: ≤ 4.5 %
  • Gel Point (1.5% Solution): 33 - 38 °C
  • Gel Strength (1.5% Gel):  800 g/cm2
  • Loss on Drying:  12.0 %
  • Melting Point (1.5 %): 88.0 - 96.0
  • pH (1.5% solution): 6.0 - 8.0
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