Molecular Biology Reagents/Chemicals

MIDSCI™ gives you ultra-pure, cost-competitive molecular biology reagents and lab chemicals for life science research; often with significant cost savings over Sigma Aldrich and other lab reagent providers.

PR1MA™ Chemicals can help with a wide variety of research-based requirements.
 
Use our glycerol for polyacrylamide gel electrophoresis. Eliminate Agrobacterium after inoculation with the carbenicillin we carry. Or detect β-Galactosidase activity with X-GAL which, when combined with the IPTG we stock, can be used to detect lac gene expression in cloning applications.
 
We also carry Luria Broth (Lennox), for the propagation and maintenance of E. coli; DTT, in order to maintain –SH groups in a reduced state; and high gel strength agar for tissue culture and microbiological work.

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PR1MA Glycine, Free Base

CAS Number: 56-40-6
Molecular Weight: 75.1
Chemical Formula: C2H5NO2
Solubility: Water
Storage Temperature: Room Temperature


For use in preparing electrophoresis buffers. Cell culture tested.


Research or further manufacturing use only, not for food or drug use.


  • Appearance: White odorless crystalline powder with sweet taste.
  • Solubility (5% in water): Clear & colorless
  • Identification (IR): Test Passes
  • Loss on Drying (@105 oC for 2 hrs):  0.20 %
  • Residue on Ignition:  0.10 %
  • Chloride (Cl):  0.007 %
  • Sulfate:  0.0065 %
  • Heavy Metals:  0.002 %
  • Hydrolyzable Substances: Test Passes
  • Related Compound (Diglycine):  0.1 %
  • Related Compound (Triglycine):  0.1 %
  • Related Compound (Glycine Anhydride):  0.1 %
  • Related Compound (Monochloro Acetic Acid):  0.1 %
  • Related Compound (Iminodiacetic Acid):  0.1 %
  • Related Compound (Haxamathylenetetraamine):  0.1 %
  • Related Compound (Total Impurities):  1.0 %
  • Residual Solvent (Chloroform):  60 mcg/gm
  • Residual Solvent (1,4 Dioxane):  380 mcg/gm
  • Residual Solvent (Methylene Chloride):  600 mcg/gm
  • Residual Solvent (Trichloroethylene):  80 mcg/gm
  • Methanol:  0.3 %
  • Assay: 98.5 - 101.5 %
  • Arsenic:  1.0 ppm
  • Total Viable Bacterial Count:  1000 Cfu/gm
  • Coliform Count: Negative
  • Yeast and Mold Count:  100 CFU/g
  • Test for E-Coli: Absent
  • Test for Salmonella: Absent
  • Test for S-Aureus: Absent
  • Test for Pseudoomonas: Absent
  • BSE/TSE Free: BSE/TSE Free
  • Grade: USP
  • Country of Origin : Report

[Dextrose Anhydrous]

PR1MA D-(+)-Glucose [Dextrose Anhydrous]

  • Common Name: Dextrose Anhydrous
  • CAS Number: 50-99-7
  • Molecular Weight: 180.16
  • Chemical Formula: C6H12O6
  • Solubility: Water
  • Storage Temperature: Room Temperature


USP Grade material.


Research or further manufacturing use only, not for food or drug use.
 
 

  • Appearance: White Crystalline Powder
  • Assay: 97.5 - 102.0 %
  • Identification (FTIR Spectrum): Pass Test
  • Identification (HPLC): Matches the reference
  • Specific Rotation: +52.6 - +53.2 °
  • Moisture:  1.0 %
  • Residue on Ignition:  0.1 %
  • Color of solution: Qualified
  • Conductivity:  20.0 µs/cm
  • Dextrins: Qualified
  • Soluble starch and sulfite: Qualified
  • Related Substance ( Maltitol and Isomalt):  0.4 %
  • Related Substance (Maltotriose):  0.2 %
  • Related Substance (Fructose):  0.15 %
  • Related Substance ( Unspecified Impurities):  0.1 %
  • Related Substance (Total impurity):  0.5 %
  • Heavy Metals:  5 ppm
  • Residual Solvents: None
  • Total Bacterial Count:  1000 cfu/g
  • Mold and Yeast:  100 cfu/g
  • E.Coli: Negative
  • Endotoxin:  1.25 EU/g
  • Source: Corn
  • Grade: Meets the current USP Grade
  • BSE/TSE Free: BSE/TSE Free
  • Country of Origin: Report

PR1MA™  Carbenicillin, Disodium Salt

CAS Number: 4800-94-6
Molecular Weight: 422.4
Chemical Formula: C17H16N2Na2O 6S
Solubility: Water
Storage Temperature: 2-8°C

USP grade


Prevents bacterial cell wall synthesis. Inhibits crosslinking of peptidoglycan by binding and inactivating transpeptidases. Highly active against gram negative bacteria. Commonly used for the elimination of Agrobacterium species after inoculation. Sensitive to B-Lactamase.
 

  • Non-toxic to plant cells
  • Plant cell culture tested
  • Hygroscopic


Research or further manufacturing use only, not for food or drug use.
 

  • Appearance: White to Off White Crystalline Powder
  • Identification, Sodium Reaction: Pass Test
  • Water:  6.0 %
  • pH: 6.50 - 8.0
  • Clarity of Solution: Clear Solution
  • Benzyl penicillin sodium:  5.0 %
  • Chromatographic Purity (HPLC):  92.0 %
  • Assay (Anhydrous):  88.0 %
  • Bacterial Endotoxins :  0.05 EU/mg
  • Iodine Absorbing Substances:  8.0 %
  • Potency (Dried basis):  770 µg/mg
  • Grade: USP
  • Country of Origin : Report

PR1MA Yeast Extract, Granulated

CAS Number: 8013-01-2
Solubility: Water
Storage Temperature: Room Temperature


Dried yeast autolysate with a high content of amino nitrogen and water soluble B complex vitamins. Material is specially granulated to reduce dust and increase solubility rate.


Suitable for Microbiology Applications.


Research or further manufacturing use only, not for food or drug use.


  • Appearance: Light Beige Powder
  • Amino Nitrogen Content:  3.5 %
  • Dry Matter:  94 %
  • Moisture :  6.0 %
  • pH (2.0% Solution @ 25 oC): 6.5 - 7.5
  • Sodium Chloride:  0.5 %
  • Total Nitrogen Content:  8.0 %
  • Non Animal Origin: The product does not come in contact with materials of animal origin.
[Miller's LB Agar]

PR1MA Luria Agar Powder [Miller's LB Agar]

Common Name: Miller's LB Agar
CAS Number: 91079-40-2; 8013-01-2; 7647-14-5; 9002-18-0
Solubility: Water with Heat
Storage Temperature: Room Temperature


For propagation and maintenance of E. Coli. High Salt Formula. Molecular Biology Grade.

  • Dissolve 40 grams in 1 liter distilled water
  • Adjust pH to 7.2
  • Sterilize by autoclaving for 15 minutes


Ingredients:

  • Peptone: 10g/L
  • Yeast: 5 g/L
  • Sodium Chloride: 10g/L
  • Agar: 15g/L


Research or further manufacturing use only, not for food or drug use.


  • DCM Appearance: Homogeneous Free Flowing,Light Beige
  • PPM Appearance: Clear, no to light ppt, light yellow
  • pH (4% solution at 25°C): 6.8 - 7.2
  • Microorganism (E. coli ATCC 23724): Good to Excellent
  • Microorganism (E. coli ATCC 33694): Good to Excellent
  • Microorganism (E. coli ATCC 33849): Fair to Excellent
  • Microorganism (E. coli ATCC 39403): Good to Excellent
  • Microorganism (E. coli ATCC 47014): Good to Excellent
  • Microorganism (E. coli ATCC 53868): Good to Excellent

PR1MA bis-Acrylamide

CAS Number: 110-26-9
Molecular Weight: 154.2
Chemical Formula: C7H10N2O2
Solubility: Water
Storage Temperature: Room Temperature


Research or further manufacturing use only, not for food or drug use.


  • Appearance: White Crystalline Powder
  • Absorbance 290nm (1% water ):  0.2
  • Acrylic Acid:  0.001 %
  • Conductivity (2% Water):  5 umhos
  • DNase: Not Detected
  • Identification: Pass
  • pH (1%, 0.1M NaCl) 25oC:  5.0
  • Protease: Not Detected
  • Purity:  99.0 %
  • RNase: Not Detected

PR1MA  Kanamycin Monosulfate [Kanamycin A]

CAS Number: 25389-94-0
Molecular Weight: 582.6
Chemical Formula: C18H36N4O11  H2SO4
Solubility: Water
Storage Temperature: Room Temperature


Aminoglycoside antibiotic with a bactericidal action against most gram negative bacteria. Incorporates into sensitive bacterial cells by an active transport process. Binds to the 30S and to some extent the 50S subunits of the bacterial ribosome, inhibiting protein synthesis and generating errors in the transcription of the genetic code. Widely used as a selective agent for the incorporation of the NPT II (APH3') gene in plant tissue.


Research or further manufacturing use only, not for food or drug use.
 

  • Appearance: White Powder
  • Chromatographic Purity : Pass Test
  • Identification: Pass Test
  • Loss on Drying:  1.5 %
  • pH (1% Solution): 6.5 - 8.5
  • Potency:  750 µg/mg
  • Residue on Ignition:  1.0 %
  • Specific Rotation: +116 - +123°

 

PR1MA Bicine Ultra-Pure

CAS Number: 150-25-4
Molecular Weight: 163.2
Chemical Formula: C6H13NO4
Solubility: Water
Storage Temperature: Room Temperature


Zwitterionic buffer used in a variety of molecular biology applications such as protein resolution, protein crystallization and SDS-PAGE.


pKa @ 25°C: 8.3
pH Range: 7.6-9.0
Melting Range: 190-192°C


Research or further manufacturing use only, not for food or drug use.

  • Appearance: White Powder
  • FTIR: Conforms to reference
  • Optical ABS @ 260 NM:  0.1
  • Optical ABS @ 280 NM:  0.1

 

PR1MA  Phosphate Buffered Saline, 10X Solution

Common Name: Phosphate Buffered Saline 10X Solution
CAS Number: 7732-18-5; 7647-14-5; 7447-40-7; 7558-79-4; 7778-77-0
Solubility: Water
Storage Temperature: Room Temperature


Phosphate Buffered Saline is a buffer solution commonly used in biological research. PBS has many uses because it is isotonic and non-toxic to most cells. These uses include substance dilution and cell container rinsing. Ready to use sterile 10X solution.


Research or further manufacturing use only, not for food or drug use.


  • Appearance: Clear and Colorless Solution
  • Composition: Sodium Chloride: 1.37M, Potassium Chloride: 0.027M, Phosphate Buffer: 0.12M
  • Method: Prepared in 18.2 megaohams-cm ± 1 water and filtered through 1 micron filter
  • pH (1X Solution): 7.2 - 7.6
  • pH (10X Solution): 6.8 - 7.2
[1,3-Diaza-2-,4-cyclopentadiene]

PR1MA Imidazole [Glyoxaline] [1,3-Diaza-2-,4-cyclopentadiene]

Common Name: [Glyoxaline] [1,3-Diaza-2,4-cyclopentadiene]
CAS Number: 288-32-4
Molecular Weight: 68.1
Chemical Formula: C3H4N2
Solubility: Water
Storage Temperature: Room Temperature


pKa (25°C):
6.9-7.0
pH range: 6.2-7.8


Research or further manufacturing use only, not for food or drug use.


  • Appearance: White to off white crystals
  • Assay:  99.0 %
  • DNase: Not Detected
  • Identification: Pass Test
  • Loss on Drying:  0.5 %
  • Melting Point: 88 - 92 oC
  • OPTICAL @ 260 NM:  0.2
  • PKa @ 25°C: 6.79 - 7.19
  • Protease: Not Detected
  • RNase: Not Detected

PR1MA 2XYT Broth, Granulated

CAS Number: 91079-40-2; 8013-01-2; 7647-14-5
Solubility: Water
Storage Temperature: Room Temperature


Nutritionally enriched medium for the growth of recombinant strains of E. Coli and in the preparation and transformation of competent cells.


Material is specially granulated to reduce dust and increase solubility rate.


Dissolve 31g in one liter distilled water. Heat solution to boiling with continuous stirring. Sterilize by autoclaving at 121°C for 15 minutes. Final pH@ 25°C: 6.8-7.2.


Ingredients:

  • Digest Peptone: 16 g/l
  • Yeast Extract: 10 g/l
  • Sodium Chloride: 5 g/l


Research or further manufacturing use only, not for food or drug use.


  • Appearance: Cream to beige free flowing granulated
  • DCM Appearance: Homogeneous free flowing, beige
  • PPM Appearance: Clear,no to light ppt,yellow
  • pH (3.1 % Solution @25 oC): 6.8 - 7.2
  • Microorganism (E. coli ATCC 23724): Good to Excellent
  • Microorganism (E. coli ATCC 33694): Good to Excellent
  • Microorganism (E. coli ATCC 33849): Good to Excellent
  • Microorganism (E. coli ATCC 39403): Good to Excellent
  • Microorganism (E. coli ATCC 47014): Good to Excellent
  • Microorganism (E. coli ATCC 53868): Good to Excellent

PR1MA  G-418 Disulfate

CAS Number: 108321-42-2
Molecular Weight: 692.7
Chemical Formula: C20H40N4O10  2H2SO4
Solubility: Water
Storage Temperature: 2-8°C
 

Aminoglycoside antibiotic used as a selective agent in transformation experiments. Alternative for Kanamycin in monocots eg: rice, lollum and graminea which are highly resistant to the latter. Optical rotation +121.2°. Cell culture tested on Bacillus subtilus.

 
Research or further manufacturing use only, not for food or drug use.

 
  • Appearance: White Powder
  • Absorbance @ 280nm (1mg/mL in H2O):  0.015 nm
  • Absorbance @ 570nm (100mg/mL in H2O):  0.10 nm
  • Ammonia:  1.0 %
  • HPLC (Purity) : Report %
  • Loss on Drying:  10.0 %
  • pH: 5.0 - 6.0
  • Potency (Dry basis):  700 µg/mg
  • Purity (TLC-Spot):  3 Spots
  • Solubility (100mg/ml in H2O): Colorless Solution
  • Specific Rotation: +104 - +121 °
  • Non Animal Origin: The product does not come in contact with materials of animal origin.
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