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Molecular Biology Reagents/Chemicals

MIDSCI™ gives you ultra-pure, cost-competitive molecular biology reagents and lab chemicals for life science research; often with significant cost savings over Sigma Aldrich and other lab reagent providers.

PR1MA™ Chemicals can help with a wide variety of research-based requirements.
 
Use our glycerol for polyacrylamide gel electrophoresis. Eliminate Agrobacterium after inoculation with the carbenicillin we carry. Or detect β-Galactosidase activity with X-GAL which, when combined with the IPTG we stock, can be used to detect lac gene expression in cloning applications.
 
We also carry Luria Broth (Lennox), for the propagation and maintenance of E. coli; DTT, in order to maintain –SH groups in a reduced state; and high gel strength agar for tissue culture and microbiological work.

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PR1MA™ 2XYT Broth, Capsules

CAS Number: 91079-40-2; 8013-01-2; 7647-14-5
Solubility: Water
Storage Temperature: Room Temperature


Nutritionally enriched medium for the growth of recombinant strains of E. Coli and in the preparation and transformation of competent cells.


2XYT Broth packaged in convenient ready to use capsules. Each capsule contains 15.5 g 2XYT Broth.


Simply add 2 capsules to one liter distilled water and heat until completely dissolved. Sterilize by autoclaving at 121°C for 15 minutes. Final pH at 25°C: 6.8-7.2.


Ingredients:

  • Digest Peptone: 16 g/l
  • Yeast Extract: 10 g/l
  • Sodium Chloride:5 g/l


Research or further manufacturing use only, not for food or drug use.


  • Appearance: Capsule (Cream to beige free flowing powder)
  • DCM Appearance: Capsule (Homogeneous, free flowing, beige)
  • PPM Appearance: Capsule (Clear, no to light ppt, yellow)
  • pH (3.1 % Solution @25 oC): 6.8 - 7.2
  • Microorganism (E. coli ATCC 23724): Good to Excellent
  • Microorganism (E. coli ATCC 33694): Good to Excellent
  • Microorganism (E. coli ATCC 33849): Good to Excellent
  • Microorganism (E. coli ATCC 39403): Good to Excellent
  • Microorganism (E. coli ATCC 47014): Good to Excellent
  • Microorganism (E. coli ATCC 53868): Good to Excellent
  • Casein Peptone: 16.0 g/L
  • Yeast Extract: 10.0 g/L
  • Sodium Chloride: 5.0 g/l
  • Description: Add 2 capsules 2XYT broth in 1 liter purified water until evenly dispersed. Boil for 1 minute with stirring to completely dissolve. Distribute and autoclave at 121oC for 15 minutes.
Item#:
AS2XYTBROTHCAP
 
PR1MA Growth Media
Safety Data Sheet for PR1MA 2XYT Broth, Capsules

PR1MA™ 2XYT Broth, Powder

CAS Number: 91079-40-2; 8013-01-2; 7647-14-5
Solubility: Water
Storage Temperature: Room Temperature


Nutritionally enriched medium for the growth of recombinant strains of E. Coli and in the preparation and transformation of competent cells.


Dissolve 31g in one liter distilled water. Heat solution to boiling with continuous stirring. Sterilize by autoclaving at 121°C for 15 minutes. Final ph @ 25°C: 6.8-7.2.


Ingredients:

  • Digest Peptone: 16 g/l
  • Yeast Extract: 10 g/l
  • Sodium Chloride: 5 g/l


Research or further manufacturing use only, not for food or drug use.


  • Appearance: Cream to beige free flowing powder
  • DCM Appearance: Homogeneous free flowing, beige
  • PPM Appearance: Clear,no to light ppt,yellow
  • pH (3.1 % Solution @25 oC): 6.8 - 7.2
  • Microorganism (E. coli ATCC 23724): Good to Excellent
  • Microorganism (E. coli ATCC 33694): Good to Excellent
  • Microorganism (E. coli ATCC 33849): Good to Excellent
  • Microorganism (E. coli ATCC 39403): Good to Excellent
  • Microorganism (E. coli ATCC 47014): Good to Excellent
  • Microorganism (E. coli ATCC 53868): Good to Excellent
Item#:
AS2XYTBROTHPOW
 
PR1MA Growth Media
Safety Data Sheet for PR1MA 2XYT Broth, Powder

PR1MA™ Tryptone, Powder

CAS Number: 91079-40-2
Solubility: Water
Storage Temperature: Room Temperature


Pancreatic digest of casein used to supplement media in cultivation of anaerobic bacteria.


pH (1% solution): 7.3.


Ingredients:

  • Molecular Biology GradeTotal Nitrogen (TN): ~12.7%
  • Amino Nitrogen (AN): ~3.7%
  • AN/TN Ratio: 0.26
  • Sodium Chloride: ~0.4%
  • Tryptophan: ~1.0


Research or further manufacturing use only, not for food or drug use.
 
  • Appearance: Cream to beige free flowing powder
  • Amino Nitrogen: ≥ 4.0 %
  • Ash: ≤ 15 %
  • Loss on Drying: ≤ 5.0 %
  • Nitrites: Negative
  • pH (2% water) @25°C: 6.5 - 7.5
  • Total Nitrogen: ≥ 10 %
  • Undigested Protein: Negative
  • Broth Formulations (Growth Supporting Properties): Satisfactory
  • Peptone Agar (Growth Supporting Properties): Satisfactory
  • Proteoses: Positive
  • Salmonella: Negative/25g
  • Standard Plate Count : ≤ 1000 cfu/g
  • Coliform: Negative
  • E.Coli: Negative
  • Molds and Yeasts: ≤ 100 cfu/g
  • Raw Material Species: Bovine
  • Enzyme Species: Porcine
  • BSE/TSE Free: BSE/TSE Free
  • Country of Origin : Report

Item#:
ASTRYPTONE
 
Safety Data Sheet for PR1MA Tryptone, Powder

PR1MA™ TBE Buffer 10x Powder

 

Common Name: TRIS-Borate-EDTA

CAS Number: 77-86-1; 10043-35-3;6381-92-6/60-00-4

Solubility: Water

Storage Temperature: Room Temperature


Dry homogeneous blend of molecular biology grade TRIS base, Boric Acid and EDTA.

For use in gel electrophoresis.

Shipping Weight: 0.5 lbs

Shipping Dimensions: 3.50 x 3.75 x 3.50

Appearance: White Powder
 
pH (1 X Solution): 8.20 - 8.40
 
Solubility: Clear and Colorless
 

Research or further manufacturing use only, not for food or drug use.
Item#:
ASTBEBUFFER10XP
 
Safety Data Sheet for PR1MA TBE Buffer 10x Powder
[N-(2-Hydroxyethyl) piperazine-N'-(2-ethanesulfonic acid)]

PR1MA™  HEPES, Sodium Salt [N-(2-Hydroxyethyl) piperazine-N'-(2-ethanesulfonic acid)]

Common Name: N-(2-Hydroxyethyl)piperazine-N'-(2-ethanesulfonic acid)
CAS Number: 75277-39-3
Molecular Weight: 260.3
Chemical Formula: C8H17N2O4SNa
Solubility: Water
Storage Temperature: Room Temperature


  • pKa (20°C): 7.45-7.65
  • pH Range: 6.8-8.2


Research or further manufacturing use only, not for food or drug use.


  • Appearance: White crystalline powder
  • Assay (Titration, dried basis): ≥ 98.0 %
  • Chloride (Cl): ≤ 0.1 %
  • Heavy Metals (as Pb): ≤ 1 ppm
  • Loss on Drying @ 105 oC, 3hrs: ≤ 2.0 %
  • pH (1% aqueous @ 25 oC): 9.4 - 11.0
  • pKa @ 20°C: 7.45 - 7.65
  • Solubility (1M Solution in H2O): Clear, complete and colorless
  • UV Absorbance @ 250nm 1M: ≤ 1.0

Item#:
ASHEPESSS
 
PR1MA Biological Buffers
Safety Data Sheet for PR1MA HEPES, Sodium Salt

PR1MA™ Hygromycin B Powder 

 
CAS Number: 31282-04-9

Molecular Weight: 527.5

Chemical Formula: C20H37N3O13

Solubility: Water

Storage Temperature: 2-8°C


Aminoglycoside antibiotic inhibits protein synthesis in prokaryote and eukaryote cells. Allows selection of cells expressing the Hygromycin B resistant gene. Working concentrations vary from 30-1000 ug/mg, depending on cell lines and growth conditions.

Shipping Weight: 0 lbs

Shipping Dimensions: 1.25 x 1.75 x 1.25



Research or further manufacturing use only, not for food or drug use.
Item#:
ASPRHYGROMYCINB
 
PR1MA Safety Data Sheet KCH75020-1_sds_H75020
[Miller's LB Broth]

 

PR1MA™ Luria Broth Granulated [Miller's LB Broth]

Common Name: Miller's LB Broth
CAS Number: 91079-40-2; 8013-01-2; 7647-14-5
Solubility: Water
Storage Temperature: Room Temperature


For propagation and maintenance of E. Coli. High Salt Formula. Molecular Biology Grade.


Dissolve 25 grams in 1 liter distilled water and adjust the pH to 7.2. Heat and frequently agitate to completely dissolve. Sterilize by autoclaving for 15 minutes. Material specially granulated to reduce dust and increase solubility rate.


Ingredients:

  • Tryptone:10 g/L
  • Sodium Chloride: 10 g/L
  • Yeast Extract: 5 g/L


Research or further manufacturing use only, not for food or drug use.


  • Appearance: Cream to beige granular powder
  • DCM Appearance: Homogeneous, free flowing beige to yellow-beige
  • PPM Appearance: Clear, yellow to amber no precipitate (ppt)
  • Appearance of Solution: Prepared medium is yellow to gold and clear to moderately hazy
  • pH (2.5% H2O @25 °C): 6.8 - 7.2
  • Microorganism (E. coli ATCC 23724): Good to Excellent
  • Microorganism (E. coli ATCC 33694): Good to Excellent
  • Microorganism (E. coli ATCC 33849): Fair to Excellent
  • Microorganism (E. coli ATCC 39403): Good to Excellent
  • Microorganism (E. coli ATCC 47014): Good to Excellent
  • Microorganism (E. coli ATCC 53868): Good to Excellent
  • Casein Peptone: 10 g/L
  • Sodium Chloride: 10 g/L
  • Yeast Extract: 5 g/L
Item#:
ASLURIABGRAN
 
PR1MA Growth Media
Safety Data Sheet for PR1MA Luria Broth Granulated [Miller's LB Broth]

PR1MA™ Urea, UltraPure (USP Grade)

CAS Number: 57-13-6
Molecular Weight: 60.06
Chemical Formula: CH4N2O
Solubility: Water
Storage Temperature: Room Temperature


Research or further manufacturing use only, not for food or drug use.


  • Appearance: White Crystalline Powder
  • Assay: 99.0 - 100.5 %
  • Identification (A, B): Pass Test
  • Appearance of 20% Solution: Pass Test
  • Melting Point: 132.5 to 134.5 oC
  • Moisture (LOD): ≤ 1.0 %
  • Alcohol- Insoluble Matter: ≤ 0.04 %
  • Residue on Ignition: ≤ 0.1 %
  • Chloride: ≤ 5 ppm
  • Sulfate: ≤ 0.001 %
  • Alkalinity: To pass test
  • Ammonium: ≤ 0.05 %
  • Urea Related Compound A: ≤ 0.1 %
  • Individual Unspecified Impurities: ≤ 0.1 %
  • Total impurities: ≤ 2.0 %
  • Heavy Metals: ≤ 0.001 %
  • Iron: ≤ 0.001 %
  • Residual Solvents USP 467: Meets requirements
  • BSE/TSE Free: BSE/TSE Free
  • Grade: USP
  • Country of Origin : Report

Item#:
ASUREAUP
 
Safety Data Sheet for PR1MA Urea, UltraPure (USP Grade)

PR1MA™ PBS/Tween 20 10X Solution

CAS Number: 7558-79-4, 7778-77-0, 7647-14-5, 7447-40-7, 9005-64-5, 7732-18-5
Solubility: Water
Storage Temperature: Room Temperature


Research or further manufacturing use only, not for food or drug use.
 

  • Appearance: Clear, yellow solution
  • pH: 7.2 - 7.6
Item#:
ASPBSTWEEN
 
PR1MA Biological Buffers
Safety Data Sheet for PR1MA PBS/Tween 20 10X Solution

PR1MA™  Cefotaxime, Sodium Salt

CAS Number: 64485-93-4
Molecular Weight: 477.5
Chemical Formula: C16H16N5NaO7S 2
Solubility: Water
Storage Temperature: 2-8°C


Inhibitor of bacterial cell wall synthesis. Inhibits the crosslinking of peptidoglycan by binding and inactivating transpeptidases. High activity against gram negative bacteria. Often used for elimination of Agrobacterium species after inoculation. Resistant to B-Lactamase activity.


Non toxic to plant cells
Plant cell culture tested


Research or further manufacturing use only, not for food or drug use.
  

  • Appearance: Off White to Pale Yellow Crystalline Powder
  • Assay (Potency dry basis) : 916 - 964 µg/mg
  • Bacterial Endotoxins : Meet the requirements
  • Clarity of Solution: Meet the requirements
  • Color of solution: Meet the requirements
  • Identification (A, B, C): Pass Test
  • Impurity - Individual: ≤ 1.0 %
  • Loss on Drying: ≤ 3.0 %
  • pH: 4.5 - 6.5
  • Specific Rotation: +58 - +64 °
  • Total impurities: ≤ 3.0 %
Item#:
ASCEFOTX
 
PR1MA Antibiotics
Safety Data Sheet for PR1MA Cefotaxime, Sodium Salt

PR1MA™  Gentamicin Sulfate

CAS Number: 1405-41-0
Molecular Weight: 575.67
Chemical Formula: C21H43N5O7 • H2SO4
Solubility: Water
Storage Temperature: Room Temperature

 
Cell culture tested. Aminoglycoside antibiotic with bactericidal activity against most gram negative bacteria.

 
Research or further manufacturing use only, not for food or drug use.


  • Appearance: White to off white powder
  • Composition (C1): 20.0 - 50.0 %
  • Composition (C1a): 10.0 - 35.0 %
  • Composition (C2a + C2): 25.0 - 55.0 %
  • Identification: Meet The Requirements
  • Loss on Drying: ≤ 18.0 %
  • Methanol: ≤ 1.0 %
  • pH: 3.5 - 5.5
  • Potency (Dried basis): ≥ 590 µg/mg
  • Residual Solvents: Meet Requirements
  • Residue on Ignition: ≤ 1.0 %
  • Specific Optical Rotation: +107 - +121 °
  • Country of Origin : Report
Item#:
ASGENTAM
 
PR1MA Antibiotics
Safety Data Sheet for PR1MA Gentamicin Sulfate

PR1MA™ Peptone, Powder

CAS Number: 73049-73-7
Solubility: Water
Storage Temperature: Room Temperature


Mixture of peptides and free amino acids from pancreatic hydrolysis of animal tissue. Low NaCl content suitable for tissue culture.


Total Nitrogen (TN): 15.0-16.0%
Amino Nitrogen (AN): 3.0-3.5%
AN/TN Ratio: 0.21-0.23


Research or further manufacturing use only, not for food or drug use.


  • Appearance: White to Light Beige Fine Powder
  • Amino Nitrogen: ≥ 3.4 %
  • Ash, Residue in Ignition: ≤ 15 %
  • Loss on Drying: ≤ 5 %
  • Nitrites: Negative
  • pH (2.0% Solution @ 25 oC): 6.5 - 7.5
  • Proteoses: Report
  • Total Nitrogen: ≥ 10 %
  • Undigested Protein: Negative
  • Coliforms: Negative
  • E.Coli: Negative
  • Molds and Yeasts: Report cfu/g
  • Salmonella : Negative cfu/25g
  • Standard Plate Count : ≤ 1000 cfu/g
  • Broth Formulations (Growth Supporting Properties): Satisfactory
  • Peptone Agar (Growth Supporting Properties): Satisfactory
  • Raw Material Species: Bovine
  • Enzyme Species: Porcine
Item#:
ASPEPTONEPOW
 
PR1MA Growth Media
Safety Data Sheet for PR1MA Peptone, Powder
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