Molecular Biology Reagents/Chemicals

MIDSCI™ gives you ultra-pure, cost-competitive molecular biology reagents and lab chemicals for life science research; often with significant cost savings over Sigma Aldrich and other lab reagent providers.

PR1MA™ Chemicals can help with a wide variety of research-based requirements.
 
Use our glycerol for polyacrylamide gel electrophoresis. Eliminate Agrobacterium after inoculation with the carbenicillin we carry. Or detect β-Galactosidase activity with X-GAL which, when combined with the IPTG we stock, can be used to detect lac gene expression in cloning applications.
 
We also carry Luria Broth (Lennox), for the propagation and maintenance of E. coli; DTT, in order to maintain –SH groups in a reduced state; and high gel strength agar for tissue culture and microbiological work.

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PR1MA Luria Broth 1 Liter Buffered Capsules [Miller's LB Broth]

Each capsules has 25 g.  The 500 g package has 20 capsules, the 1 KG is 40 capsules and the 5 KG is 200 capsules.
 

CAS Number: 91079-40-2; 8013-01-2; 7647-14-5; 77-86-1; 1185-53-1
Storage Temperature: Room Temperature
Solubility: Water
 

RPI Media is now available in convenient pre-measured and pre-mixed capsules. Pre-buffered media eliminates messy weighing and pH adjustments.


Simply dissolve 1 capsule per 1 Liter of distilled water. Water must be 37°C or warmer to completely dissolve gelatin capsule. Agitate. Sterilize by autoclaving for 15 minutes before use. Buffer is set for 7.2 at 20°C using a 10mM solution of Tris/Tris HCl. pH will vary depending on temperature.


For maintenance and propagation of E. Coli. High Salt Formula. Molecular Biology Grade.


Ingredients:

  • Tryptone: 10 g/L
  • Sodium Chloride: 10 g/L
  • Yeast Extract: 5 g/L
  • Tris/Tris HCl: 1.5 g/L


Research or further manufacturing use only, not for food or drug use.

 

  • Appearance: Capsules (cream to pale beige free flowing powder)
  • pH (2.5 % Solution @25 oC): 6.8 - 7.2
  • Microorganism (E. coli ATCC 23724): Good to Excellent
  • Microorganism (E. coli ATCC 33694): Good to Excellent
  • Microorganism (E. coli ATCC 33849): Fair to Excellent
  • Microorganism (E. coli ATCC 39403): Good to Excellent
  • Microorganism (E. coli ATCC 47014): Good to Excellent
  • Microorganism (E. coli ATCC 53868): Good to Excellent

PR1MA™ Tris-Acetate


Molecular Weight: 181.19

Chemical Formula: C14H11NO3C2H4O2

Solubility: Water

Storage Temperature: Room Temperature


Moisture: <0.3%
pH (0.1M, H2O) @25°C: 6.0-7.0%
Heavy Metals: 0.001%

Shipping Weight: 0.3 lbs

Shipping Dimensions: 3.50 x 3.75 x 3.50

  • Appearance: White crystalline powder
  • Heavy Metals: ≤ 0.001 %
  • Moisture: ≤ 0.3 %
  • pH (0.1M Solution in water): 6.0 - 7.0
  • Purity: ≥ 98.0 %
  • Residue on Ignition: ≤ 0.1 %
  • BSE/TSE Free: BSE/TSE Free
  • Country of Origin: Report
 
Item#:
ASTRISACETATE

PR1MA™ Potato Dextrose Agar

 

CAS Number: 9012-36-6
Storage Temperature: Room Temperature
 

Potato Dextrose Agar (PDA) is a versatile medium used for cultivating fungi, yeasts, and molds. It can be modified with acid or antibiotics to prevent bacterial growth and is commonly used for plate count methods in food, dairy, and cosmetic testing. PDA is suitable for growing clinically important yeast and molds. The nutrient-rich potato infusion in PDA promotes mold sporulation and pigment production in certain dermatophytes.
Research or further manufacturing use only, not for food or drug use.

 

  • Microorganism (Aspergillus niger ATCC 16404): Good to Excellent
  • Microorganism (Candida albicans ATCC 10231): Good to Excellent
  • Microorganism (Penicillium Roquefortii ATCC 10110): Good to Excellent
  • Microorganism (Saccharomyces cerevisiae ATCC 9763): Good to Excellent
  • Microorganism (Trichophyton interdigitale ATCC 9533): Good to Excellent
  • pH (4.04% H2O @25°C): 5.4 - 5.8
 
 

PR1MA™ Terrific Broth, Modified, Powder

CAS Number: 91079-40-2; 8013-01-2; 7778-77-0; 7758-11-4
Solubility: Water
Storage Temperature: Room Temperature


Enriched media for maintenance and propagation of E. coli and plasmid growth. Produces higher cell densities resulting in greater yields of DNA or recombinant problem. Glycerol is used as a carbon source with this formulation instead of glucose as it will not ferment to acetic acid.


Ingredients:

  • Casein Digest Peptone: 12 g/L
  • Yeast Extract: 24 g/L
  • Dipotassium phosphate: 9.4 g/L
  • Monopotassium phosphate: 2.2 g/L


Research or further manufacturing use only, not for food or drug use.


  • DCM Appearance: Homogeneous Free Flowing, Beige to Yellow Beige
  • PPM Appearance: Clear Yellow to Amber ,No PPT
  • pH (4.76% solution at 25°C): 6.8 - 7.4
  • Microorganism (E. coli ATCC 23724): Good to Excellent
  • Microorganism (E. coli ATCC 33694): Good to Excellent
  • Microorganism (E. coli ATCC 33849): Good to Excellent
  • Microorganism (E. coli ATCC 39403): Good to Excellent
  • Microorganism (E. coli ATCC 47014): Good to Excellent
  • Microorganism (E. coli ATCC 53868): Good to Excellent

PR1MA Phleomycin, Powder


CAS Number: 11006-33-0
Molecular Weight: 1525
Chemical Formula: C55H85O21N202Cu 
 HCl
Solubility: Water
Storage Temperature: 2-8°C


Produced by Streptomyces verticullus and part of the structurally related group of Bleomycin/Phleomycin type antibiotics. Used as a selective agent in transformation experiments with mammalian cells, plant cells, and yeast. Cytotoxic action of this type of antibiotic results from the ability to cause fragmentation of DNA. In Vitro studies indicate that Phleomycin causes the accumulation of cells in the G2 phase of the cell cycle.

Research or further manufacturing use only, not for food or drug use.

  • Appearance: Light Blue Powder
  • U.V. Absorbance (244nm, 600 µg/ml, 0.1cm Path): 0.20 - 0.30
  • Storage Condition: Store at 2 - 8 oC (Very Hygroscopic: Keep container tightly sealed until ready for use).

 

PR1MA DTT [DL-Dithiothreitol] (Cleland's Reagent)]

Common Name: [DL-Dithiothreitol] (Cleland's Reagent)]
CAS Number: 3483-12-3
Molecular Weight: 154.25
Chemical Formula: C4H10O2S2
Solubility: Water
Storage Temperature: 2-8°C


Protective reagent for maintaining -SH groups in reduced state. DTT permeates the cell membrane protecting protein sulfhydryls thereby restoring enzyme activity lost by oxidation of the groups.


Research or further manufacturing use only, not for food or drug use.


  • Appearance: A white free flowing powder, hygroscopic
  • Solubility: Freely soluble in methanol, methylene chloride
  • Clarity in water: 5% (w/v) solution in water is clear and colorless
  • Identification by TLC: Rf value of the sample is comparable with that of the standard
  • Melting Range: 37.0 - 44.0 °C
  • Related Substance (UV Spectroscopy- Content of Oxidised DTT):  0.4 %
  • Assay (By Iodimetry):  98.0 %
  • Country of Origin : Report

PR1MA  Sodium Phosphate Dibasic, Anhydrous

CAS Number: 7558-79-4
Molecular Weight: 142.0
Chemical Formula: Na2HPO4
Solubility: Water
Storage Temperature: Room Temperature


Research or further manufacturing use only, not for food or drug use.
 

  • Appearance: White Crystal
  • Assay:  99.0 %
  • Chloride (Cl):  0.002 %
  • Heavy Metals :  10 ppm
  • Insoluble Matter:  0.01 %
  • Iron:  0.002 %
  • Loss on Drying:  0.2 %
  • pH (5% Solution): 8.7 - 9.3
  • Sulfate:  0.005 %
  • Aerobic Plate Count:  1000 CFU/g
  • Yeast and Mold :  100 cfu/g
  • Total Coliforms: Negative

 

PR1MA  Luria Broth, High Salt Formula, Powder [Miller's LB Broth]

Common Name: Miller's LB Broth
CAS Number: 91079-40-2, 8013-01-2, 7647-14-5
Solubility: Water
Storage Temperature: Room Temperature


For propagation and maintenance of E. Coli. High Salt Grade. Molecular Biology Grade.

  • Dissolve 25 grams in 1 liter distilled water
  • Adjust the pH to 7.2
  • Sterilize by autoclaving for 15 minutes


Ingredients:

  • Tryptone: 10 g/L
  • Yeast: 5 g/L
  • Sodium Chloride: 10 g/L


Research or further manufacturing use only, not for food or drug use.


  • DCM Appearance: Homogeneous free flowing, light beige
  • PPM Appearance: Clear,no to light ppt, light yellow
  • pH (2.5% H2O @25 °C): 6.8 - 7.2
  • Microorganism (E. coli ATCC 23724): Good to Excellent
  • Microorganism (E. coli ATCC 33694): Good to Excellent
  • Microorganism (E. coli ATCC 33849): Good to Excellent
  • Microorganism (E. coli ATCC 39403): Good to Excellent
  • Microorganism (E. coli ATCC 47014): Good to Excellent
  • BSE/TSE Free: BSE/TSE Free
  • Country of Origin : USA

PR1MA™ Chloramphenicol 

CAS Number: 56-75-7
Molecular Weight: 323.1
Chemical Formula: C11H12Cl2N2O 5
Solubility: Ethanol, Methanol and Slightly Soluble in Water
Storage Temperature: Room Temperature

 
Bacteriostatic antibiotic with activity against gram positive and gram negative bacteria.

 
Plant cell culture tested.

 
Research or further manufacturing use only, not for food or drug use.

 

  • Appearance: White, greyish-white or yellowish-white, fine, crystalline powder or fine crystals, needles or elongated plates
  • Identification: Pass
  • Melting Point : 149 - 153 oC
  • pH: 4.5 - 7.5
  • Specific Optical Rotation: +17.0 - +20.0o
  • Chloride:  0.01 %
  • Related Substances:  0.50 %
  • Loss on Drying:  0.50 %
  • Sulfated Ash:  0.10 %
  • Assay (Dried Basis): 98.0 - 102.0 %
  • Non Animal Origin: Non Animal Origin
  • Grade: USP
  • Country of Origin : Report

PR1MA  Cycloheximide

CAS Number: 66-81-9
Molecular Weight: 281.35
Chemical Formula: C15H23NO4
Solubility: Ethanol, methanol
Storage Temperature: 2-8°C


Antifungal antibiotic that inhibits protein synthesis in eukaryotes. Blocks translation of mRNA on the ribosome derived from Streptomyces griseus.


WARNING! This product can expose you to Cycloheximide, a chemical which is known to the State of California to cause developmental harm. For more information go to www.P65Warnings.ca.gov.


Research or further manufacturing use only, not for food or drug use.
 

  • Appearance: White to Off White Crystalline Powder
  • HPLC (Purity):  96.0 %
  • Identification (HPLC): Conforms
  • Melting Point: 102 - 120 oC
  • Potency:  950 µg/mg
  • Solubility: Soluble In Chloroform; Ethanol, Methanol
  • Water Content : < 0.3 %



 

PR1MA Acrylamide/bis-Acrylamide, 19:1 Ratio Solution

CAS Number: 79-06-1; 110-26-9; 7732-18-5
Storage Temperature: 2-8°C


A 40% solution containing 38% (w/v) Acrylamide and 2% (w/v) bis-Acrylamide for a monomer to crosslinker ratio of 19:1.


WARNING! This product can expose you to Acrylamide, a chemical which is known to the State of California to cause cancer, developmental and reproductive harm. For more information go to www.P65Warnings.ca.gov.


Research or further manufacturing use only, not for food or drug use.


  • Appearance: Clear, colorless liquid
  • Conductivity of Solution:  10 µmhos
  • DNase/ RNase: Not Detected
  • Electrophoresis: Pass Test
  • pH: Report

PR1MA Luria Broth 1 Gram Buffered Capsules [Miller's LB Broth]

Common Name: Miller's LB Broth
CAS Number: 91079-40-2; 8013-01-2; 7647-14-5; 77-86-1/1185-53-1
Solubility: Water
Storage Temperature: Room Temperature


High salt formula. Molecular Biology Grade. For maintenance and propagation of E. Coli.


RPI Media is now available in convenient pre-measured and pre-mixed capsules. Prebuffered media eliminates messy weighing and pH adjustments.


Simply dissolve 25 capsules per 1 Liter of distilled water. Water must be 37°C or warmer to completely dissolve gelatin capsule. Agitate. Sterilize by autoclaving for 15 minutes before use. Buffer is set for 7.0 ± 0.2 at 25°C using a 10mM solution of Tris/Tris HCl. pH will vary depending on temperature.


Ingredients:

  • Tryptone: 10 g/L
  • Sodium Chloride: 10 g/L
  • Yeast Extract: 5 g/L
  • Tris/Tris HCl: 1.5 g/L



Research or further manufacturing use only, not for food or drug use.


  • Appearance: Capsules
  • pH (2.5% H2O @25 °C): 6.8 - 7.2
  • Microorganism (E. coli ATCC 23724): Growth
  • Microorganism (E. coli ATCC 33694): Growth
  • Microorganism (E. coli ATCC 33849): Growth
  • Microorganism (E. coli ATCC 39403): Growth
  • Microorganism (E. coli ATCC 47014): Growth
  • Microorganism (E. coli ATCC 53868): Growth
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