Molecular Biology Reagents/Chemicals

MIDSCI™ gives you ultra-pure, cost-competitive molecular biology reagents and lab chemicals for life science research; often with significant cost savings over Sigma Aldrich and other lab reagent providers.

PR1MA™ Chemicals can help with a wide variety of research-based requirements.
 
Use our glycerol for polyacrylamide gel electrophoresis. Eliminate Agrobacterium after inoculation with the carbenicillin we carry. Or detect β-Galactosidase activity with X-GAL which, when combined with the IPTG we stock, can be used to detect lac gene expression in cloning applications.
 
We also carry Luria Broth (Lennox), for the propagation and maintenance of E. coli; DTT, in order to maintain –SH groups in a reduced state; and high gel strength agar for tissue culture and microbiological work.

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PR1MA  G-418 Disulfate 50 mg/ml Solution

CAS Number: 108321-42-2
Molecular Weight: 692.7
Chemical Formula: C20H40N4O10  2H2SO4
Solubility: Water
Storage Temperature: 2-8°C

 
Aminoglycoside antibiotic used as a selective agent in transformation experiments.

 
Dissolved in Molecular Biology Grade Water and sterile filtered.

 
Research or further manufacturing use only, not for food or drug use.

 
  • Appearance: Clear Colorless Solution
  • Absorbance @ 280nm (1mg/mL in H2O):  0.015 nm
  • Absorbance @ 570nm (100mg/mL in H2O):  0.10 nm
  • Potency (Dried basis):  700 µg/mg
  • Sterility: Filtered through 0.22 µm filter Into sterile PETG bottles

PR1MA  TAE 10X Solution [TRIS Acetate EDTA 10X Solution]

Common Name: TRIS Acetate EDTA 10X Solution
CAS Number: 77-86-1, 64-19-7, 6381-92-6, 7732-18-5
Solubility: Water
Storage Temperature: Room Temperature


10X solution contains:

  • TRIS-Base: 0.4 M
  • Acetic Acid: 0.2 M
  • EDTA: 10 mM
  • pH (10X Solution (@25°C): 8.2-8.4

Research or further manufacturing use only, not for food or drug use.
 

  • Appearance: Clear, Colorless Solution
  • pH: 7.8 - 8.2

PR1MA p-Amino Benzoic Acid [Vitamin H1]

Common Name: Vitamin H1

CAS Number: 150-13-0

Molecular Weight: 137.1

Chemical Formula: C7H7NO2

Solubility: Water

Storage Temperature: Room Temperature


Research or further manufacturing use only, not for food or drug use.
 

  • Appearance: White crystalline powder
  • Assay:  98.5 %
  • Loss on Drying:  0.20 %
  • Melting Point: 184 - 191 oC

 
PR1MA
 Doxorubicin Hydrochloride, Powder

CAS Number: 25316-40-9
Molecular Weight: 580.0
Chemical Formula: C27H29NO11 
 HCl
Solubility: Water
Storage Temperature: 2-8°C
 

Antineoplastic antibiotic interferes with nucleic acid synthesis. Also acts on cell membranes.


WARNING! This product can expose you to Doxorubicin HCl, a chemical which is known to the State of California to cause developmental harm. For more information go to www.P65Warnings.ca.gov.


Research or further manufacturing use only, not for food or drug use.
 

  • Appearance: Orange red crystalline powder
  • Assay (Anhydrous): 98.0 - 102.0 %
  • pH: 4.0 - 5.5
  • Solubility: Soluble in water; slightly soluble in methanol; practically insoluble in acetone and ethanol
  • Water:  4.0 %
  • Bacterial Endotoxins:  2.2 U/mg
  • Chloride: Complies with standard
  • Crystallinity: Meets requirements
  • FTIR: Complies with standard
  • HPLC: Complies with standard
  • 1-Butanol:  0.5 %
  • Acetone:  0.5 %
  • Doxorubicinone:  0.50 %
  • Ethanol:  1.0 %
  • Other impurities:  0.10 %
  • Total impurities:  1.0 %
  • BSE/TSE Free: BSE/TSE Free
  • Country of Origin : Report

 

 

 

PR1MA LB Broth 1 Gram Buffered Capsules [Lennox L Broth]

Common Name: Lennox L Broth
Solubility: Water
Storage Temperature: Room Temperature


RPI media is now available in convenient premeasured and pre-mixed capsules. Prebuffered media eliminates pH adjustments, dust and smells from the lab. For propagation and maintenance of E.Coli. Low Salt Formula. Molecular Biology Grade.


Add 20 capsules per liter of water. Heat and stir until capsule is completely dissolved. Buffer is set for 7.2 at 20°C using a 10mM solution of TRIS/TRIS HCl. Sterilize by autoclaving for 15 minutes.


Ingredients:

  • Tryptone: 10 g/L
  • Yeast Extract: 5 g/L
  • Sodium Chloride: 5 g/L



Research or further manufacturing use only, not for food or drug use.


  • Appearance: Capsules
  • Appearance of Solution: Prepared medium is yellow to gold and clear to moderately hazy
  • pH (2.15% solution @25°C): 6.8 - 7.2
  • Microorganism (E. coli ATCC 23724): Good to Excellent
  • Microorganism (E. coli ATCC 33694): Good to Excellent
  • Microorganism (E. coli ATCC 33849): Fair to Excellent
  • Microorganism (E. coli ATCC 39403): Good to Excellent
  • Microorganism (E. coli ATCC 47014): Good to Excellent
  • Microorganism (E. coli ATCC 53868): Good to Excellent

PR1MA  D-Cycloserine

CAS Number: 68-41-7
Molecular Weight: 102.1
Chemical Formula: C3H6N2O2
Solubility: Water
Storage Temperature: 2-8°C


Interferes with bacterial cell wall synthesis by competing with D-Alanine for incorporation into the cell wall. Active against most mycobacteria with some activity against gram negative bacteria.


Research or further manufacturing use only, not for food or drug use.
 

  • Appearance: White to pale yellowish crystalline powder
  • Identification: A blue color gradually develops
  • Specific Rotation: +108 - +114 °
  • Polymer (285nm):  0.80
  • Crystallinity: Should Comply
  • pH: 5.5 - 6.5
  • Loss on Drying:  1.0 %
  • Residue on Ignition:  0.50 %
  • Methanol:  0.30 %
  • Ethyl Acetate:  0.50 %
  • Ethanol:  0.5 %
  • Acetone:  0.50 %
  • Acetic Acid:  0.50 %
  • Assay:  90.0 %
  • Grade: USP
  • Country of Origin : Report

PR1MA Chloramphenicol

CAS Number: 56-75-7
Molecular Weight: 323.1
Chemical Formula: C11H12Cl2N25
Solubility: Ethanol, Methanol and Slightly Soluble in Water
Storage Temperature: Room Temperature


Bacteriostatic antibiotic with activity against gram-positive and gram-negative bacteria.

Plant cell culture tested.


Research or further manufacturing use only, not for food or drug use.

  • Appearance: White, greyish-white or yellowish-white, fine, crystalline powder or fine crystals, needles or elongated plates
  • Identification: Pass
  • Melting Point : 149 - 153 oC
  • pH: 4.5 - 7.5
  • Specific Optical Rotation: +17.0 - +20.0 o
  • Chloride:  0.01 %
  • Related Substances:  0.50 %
  • Loss on Drying:  0.50 %
  • Sulfated Ash:  0.10 %
  • Assay (Dried Basis): 98.0 - 102.0 %
  • Non Animal Origin: Non Animal Origin
  • Grade: USP
  • Country of Origin : Report

 

PR1MA NZCYM Broth

Solubility: Water
Storage Temperature: Room Temperature


Enriched medium for cultivating recombinant strains of E.Coli and propagating theta bacteriophage.


Dissolve 22 grams in 1 liter distilled water. Sterilize by autoclaving for 15 minutes.


Casein Digest Protein: 10 g/L
Yeast Extract: 5 g/L
Casamino Acids: 1 g/L
Magnesium Sulfate: 0.98 g/L
Sodium Chloride: 5 g/L


Research or further manufacturing use only, not for food or drug use.


  • DCM Appearance: Homogeneous, free flowing, light beige
  • PPM Appearance: Clear, No to light ppt, light yellow
  • pH (2.2 % Solution): 6.8 - 7.2
  • Escherichia coli ATCC 23724: Good to Excellent
  • Escherichia coli ATCC 33694: Good to Excellent
  • Escherichia coli ATCC 33849: Good to Excellent
  • Escherichia coli ATCC 39403: Good to Excellent
  • Escherichia coli ATCC 47014: Good to Excellent
  • Escherichia coli ATCC 53868: Good to Excellent
  • Country of Origin : Report

PR1MA Nutrient Broth

Solubility: Water
Storage Temperature: Room Temperature


For cultivating a variety of non-fastidious organisms.


Dissolve 8 grams in one liter distilled water. Heat solution to boiling with continuous stirring for one minute. Sterilize by autoclaving at 121°C for 15 minutes. Store at room temperature.

Beef Extract: 3 g/L
Pancreatic Digest of Gelatin: 5 g/L


Research or further manufacturing use only, not for food or drug use.


  • Appearance: Homogeneous Free Flowing,Beige Powder
  • pH (0.8 % Solution @25 oC): 6.6 - 7.0
  • Microorganism (Bacillus Subtlis ATCC 9372): Fair to Good
  • Microorganism (Escherichia Coli ATCC 25922): Good to Excellent
  • Microorganism (Salmonella Typhimurium ATCC 14028 ): Fair to Excellent
  • Microorganism (Staphylcoccus Aureus ATCC 25923): Good to Excellent
  • Pancreatic digest of gelatin: 5.0 g/L
  • Beef Extract: 3.0 g/L
Premixed Powder, 50:1 Ratio

PR1MA Acrylamide/bis-Acrylamide, Premixed Powder, 50:1 Ratio

Storage Temperature: Room Temperature
 

WARNING! This product can expose you to Acrylamide, a chemical which is known to the State of California to cause cancer, developmental and reproductive harm. For more information go to www.P65Warnings.ca.gov.

Research or further manufacturing use only, not for food or drug use.
 

  • Appearance: Powder
  • Solubility: Clear Solution
  • Conductivity: ≤ 10 µmhos
  • Electrophoresis: Pass

PR1MA Ethanol, Reagent Grade

Appearance: Clear Colorless Free of Foreign Matter
Color Value: ≤ 15
Ethyl Alcohol, Denatured:
Conforms Specific Gravity: 0.782 - 0.802
 

Components:

  • Ethyl Alcohol      90%
  • 2-Propanol          5%
  • Methanol            5%

PR1MA D-(+)-Biotin [Vitamin H]

Common Name: Vitamin H

CAS Number: 58-85-5

Molecular Weight: 244.3

Chemical Formula: C10H16N2O3S

Solubility: Water

Storage Temperature: 2-8°C


Research or further manufacturing use only, not for food or drug use.
 

  • Appearance: White or off white powder
  • Assay: 97.5 - 102.0 %
  • Related Substance (HPLC-Individual Impurity):  1.0 %
  • Related Substance (HPLC-Total Impurities):  2.0 %
  • Residual Solvents: To meet requirments
  • Specific Rotation: +89 - +93 °
  • BSE/TSE Free: BSE/TSE Free
  • Grade: USP

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