Molecular Biology Reagents/Chemicals

MIDSCI™ gives you ultra-pure, cost-competitive molecular biology reagents and lab chemicals for life science research; often with significant cost savings over Sigma Aldrich and other lab reagent providers.

PR1MA™ Chemicals can help with a wide variety of research-based requirements.
 
Use our glycerol for polyacrylamide gel electrophoresis. Eliminate Agrobacterium after inoculation with the carbenicillin we carry. Or detect β-Galactosidase activity with X-GAL which, when combined with the IPTG we stock, can be used to detect lac gene expression in cloning applications.
 
We also carry Luria Broth (Lennox), for the propagation and maintenance of E. coli; DTT, in order to maintain –SH groups in a reduced state; and high gel strength agar for tissue culture and microbiological work.

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PR1MA Nutrient Broth

Solubility: Water
Storage Temperature: Room Temperature


For cultivating a variety of non-fastidious organisms.


Dissolve 8 grams in one liter distilled water. Heat solution to boiling with continuous stirring for one minute. Sterilize by autoclaving at 121°C for 15 minutes. Store at room temperature.

Beef Extract: 3 g/L
Pancreatic Digest of Gelatin: 5 g/L


Research or further manufacturing use only, not for food or drug use.


  • Appearance: Homogeneous Free Flowing,Beige Powder
  • pH (0.8 % Solution @25 oC): 6.6 - 7.0
  • Microorganism (Bacillus Subtlis ATCC 9372): Fair to Good
  • Microorganism (Escherichia Coli ATCC 25922): Good to Excellent
  • Microorganism (Salmonella Typhimurium ATCC 14028 ): Fair to Excellent
  • Microorganism (Staphylcoccus Aureus ATCC 25923): Good to Excellent
  • Pancreatic digest of gelatin: 5.0 g/L
  • Beef Extract: 3.0 g/L
Premixed Powder, 50:1 Ratio

PR1MA Acrylamide/bis-Acrylamide, Premixed Powder, 50:1 Ratio

Storage Temperature: Room Temperature
 

WARNING! This product can expose you to Acrylamide, a chemical which is known to the State of California to cause cancer, developmental and reproductive harm. For more information go to www.P65Warnings.ca.gov.

Research or further manufacturing use only, not for food or drug use.
 

  • Appearance: Powder
  • Solubility: Clear Solution
  • Conductivity: ≤ 10 µmhos
  • Electrophoresis: Pass

PR1MA Ethanol, Reagent Grade

Appearance: Clear Colorless Free of Foreign Matter
Color Value: ≤ 15
Ethyl Alcohol, Denatured:
Conforms Specific Gravity: 0.782 - 0.802
 

Components:

  • Ethyl Alcohol      90%
  • 2-Propanol          5%
  • Methanol            5%

Item#:
ASETHANOL

 

PR1MA Agar, Phyto

CAS Number: 9002-18-0
Solubility: Water
Storage Temperature: Room Temperature


Specially selected and tested agar for use in plant tissue culture. High gel strength.


Concentration of 5.0 g/l obtains a solid gel strength: (1600 g/cm2).


Research or further manufacturing use only, not for food or drug use.


  • Appearance: Pale Yellow Powder
  • Gelling Point: 34 - 36 °C
  • Gel Strength : 950 - 1050 g/cm2
  • Identification: Conforms
  • Loss on Drying:  18 %
  • Melting Point: 85 - 95 oC
  • Nephelos:  40 NTU
  • pH: 6.0 - 7.5
  • Total Ash:  3.5 %
  • Gelatin: Absent
  • Starch: Absent
  • Total Plate Count:  1000 cfu/g

PR1MA D-(+)-Biotin [Vitamin H]

Common Name: Vitamin H

CAS Number: 58-85-5

Molecular Weight: 244.3

Chemical Formula: C10H16N2O3S

Solubility: Water

Storage Temperature: 2-8°C


Research or further manufacturing use only, not for food or drug use.
 

  • Appearance: White or off white powder
  • Assay: 97.5 - 102.0 %
  • Related Substance (HPLC-Individual Impurity):  1.0 %
  • Related Substance (HPLC-Total Impurities):  2.0 %
  • Residual Solvents: To meet requirments
  • Specific Rotation: +89 - +93 °
  • BSE/TSE Free: BSE/TSE Free
  • Grade: USP

PR1MA Luria Broth 1 Liter Buffered Capsules [Miller's LB Broth]

Each capsules has 25 g.  The 500 g package has 20 capsules, the 1 KG is 40 capsules and the 5 KG is 200 capsules.
 

CAS Number: 91079-40-2; 8013-01-2; 7647-14-5; 77-86-1; 1185-53-1
Storage Temperature: Room Temperature
Solubility: Water
 

RPI Media is now available in convenient pre-measured and pre-mixed capsules. Pre-buffered media eliminates messy weighing and pH adjustments.


Simply dissolve 1 capsule per 1 Liter of distilled water. Water must be 37°C or warmer to completely dissolve gelatin capsule. Agitate. Sterilize by autoclaving for 15 minutes before use. Buffer is set for 7.2 at 20°C using a 10mM solution of Tris/Tris HCl. pH will vary depending on temperature.


For maintenance and propagation of E. Coli. High Salt Formula. Molecular Biology Grade.


Ingredients:

  • Tryptone: 10 g/L
  • Sodium Chloride: 10 g/L
  • Yeast Extract: 5 g/L
  • Tris/Tris HCl: 1.5 g/L


Research or further manufacturing use only, not for food or drug use.

 

  • Appearance: Capsules (cream to pale beige free flowing powder)
  • pH (2.5 % Solution @25 oC): 6.8 - 7.2
  • Microorganism (E. coli ATCC 23724): Good to Excellent
  • Microorganism (E. coli ATCC 33694): Good to Excellent
  • Microorganism (E. coli ATCC 33849): Fair to Excellent
  • Microorganism (E. coli ATCC 39403): Good to Excellent
  • Microorganism (E. coli ATCC 47014): Good to Excellent
  • Microorganism (E. coli ATCC 53868): Good to Excellent

PR1MA™ Terrific Broth, Modified, Powder

CAS Number: 91079-40-2; 8013-01-2; 7778-77-0; 7758-11-4
Solubility: Water
Storage Temperature: Room Temperature


Enriched media for maintenance and propagation of E. coli and plasmid growth. Produces higher cell densities resulting in greater yields of DNA or recombinant problem. Glycerol is used as a carbon source with this formulation instead of glucose as it will not ferment to acetic acid.


Ingredients:

  • Casein Digest Peptone: 12 g/L
  • Yeast Extract: 24 g/L
  • Dipotassium phosphate: 9.4 g/L
  • Monopotassium phosphate: 2.2 g/L


Research or further manufacturing use only, not for food or drug use.


  • DCM Appearance: Homogeneous Free Flowing, Beige to Yellow Beige
  • PPM Appearance: Clear Yellow to Amber ,No PPT
  • pH (4.76% solution at 25°C): 6.8 - 7.4
  • Microorganism (E. coli ATCC 23724): Good to Excellent
  • Microorganism (E. coli ATCC 33694): Good to Excellent
  • Microorganism (E. coli ATCC 33849): Good to Excellent
  • Microorganism (E. coli ATCC 39403): Good to Excellent
  • Microorganism (E. coli ATCC 47014): Good to Excellent
  • Microorganism (E. coli ATCC 53868): Good to Excellent

PR1MA Phleomycin, Powder


CAS Number: 11006-33-0
Molecular Weight: 1525
Chemical Formula: C55H85O21N202Cu 
 HCl
Solubility: Water
Storage Temperature: 2-8°C


Produced by Streptomyces verticullus and part of the structurally related group of Bleomycin/Phleomycin type antibiotics. Used as a selective agent in transformation experiments with mammalian cells, plant cells, and yeast. Cytotoxic action of this type of antibiotic results from the ability to cause fragmentation of DNA. In Vitro studies indicate that Phleomycin causes the accumulation of cells in the G2 phase of the cell cycle.

Research or further manufacturing use only, not for food or drug use.

  • Appearance: Light Blue Powder
  • U.V. Absorbance (244nm, 600 µg/ml, 0.1cm Path): 0.20 - 0.30
  • Storage Condition: Store at 2 - 8 oC (Very Hygroscopic: Keep container tightly sealed until ready for use).

 

Kappa is now PR1MA - Same great product, new name
Looking for something else? Email tech@midsci.com. If we can get it, we will add it to the website.

 

PR1MA Agarose 500mg Tablets

CAS Number: 9012-36-6
Solubility: Water
Storage Temperature: Room Temperature


Save time and labor preparing agarose gels for electrophoresis of nucleic acids. Just place the required number of tablets into a standard buffer, then mix and heat until completely dissolved.


Tablet Weight Range: 485-515 mg
Gel Strength, 1.5%: >1200 g/cm2
Gelling Range: 36-39°C
Melting Temp: 87-89°C
EEO: <0.10
Sulfate: <0.15
DNase, RNase: None Detected


Research or further manufacturing use only, not for food or drug use.
 

  • Appearance: White tablets
  • Gelling Range (1.5% Water): 36 - 39 oC
  • pH (3% water, 25°C): 5.7 - 7.7
  • Tablet Weight: 485 - 515 mg

PR1MA DTT [DL-Dithiothreitol] (Cleland's Reagent)]

Common Name: [DL-Dithiothreitol] (Cleland's Reagent)]
CAS Number: 3483-12-3
Molecular Weight: 154.25
Chemical Formula: C4H10O2S2
Solubility: Water
Storage Temperature: 2-8°C


Protective reagent for maintaining -SH groups in reduced state. DTT permeates the cell membrane protecting protein sulfhydryls thereby restoring enzyme activity lost by oxidation of the groups.


Research or further manufacturing use only, not for food or drug use.


  • Appearance: A white free flowing powder, hygroscopic
  • Solubility: Freely soluble in methanol, methylene chloride
  • Clarity in water: 5% (w/v) solution in water is clear and colorless
  • Identification by TLC: Rf value of the sample is comparable with that of the standard
  • Melting Range: 37.0 - 44.0 °C
  • Related Substance (UV Spectroscopy- Content of Oxidised DTT):  0.4 %
  • Assay (By Iodimetry):  98.0 %
  • Country of Origin : Report

PR1MA  Sodium Phosphate Dibasic, Anhydrous

CAS Number: 7558-79-4
Molecular Weight: 142.0
Chemical Formula: Na2HPO4
Solubility: Water
Storage Temperature: Room Temperature


Research or further manufacturing use only, not for food or drug use.
 

  • Appearance: White Crystal
  • Assay:  99.0 %
  • Chloride (Cl):  0.002 %
  • Heavy Metals :  10 ppm
  • Insoluble Matter:  0.01 %
  • Iron:  0.002 %
  • Loss on Drying:  0.2 %
  • pH (5% Solution): 8.7 - 9.3
  • Sulfate:  0.005 %
  • Aerobic Plate Count:  1000 CFU/g
  • Yeast and Mold :  100 cfu/g
  • Total Coliforms: Negative

 

PR1MA  Luria Broth, High Salt Formula, Powder [Miller's LB Broth]

Common Name: Miller's LB Broth
CAS Number: 91079-40-2, 8013-01-2, 7647-14-5
Solubility: Water
Storage Temperature: Room Temperature


For propagation and maintenance of E. Coli. High Salt Grade. Molecular Biology Grade.

  • Dissolve 25 grams in 1 liter distilled water
  • Adjust the pH to 7.2
  • Sterilize by autoclaving for 15 minutes


Ingredients:

  • Tryptone: 10 g/L
  • Yeast: 5 g/L
  • Sodium Chloride: 10 g/L


Research or further manufacturing use only, not for food or drug use.


  • DCM Appearance: Homogeneous free flowing, light beige
  • PPM Appearance: Clear,no to light ppt, light yellow
  • pH (2.5% H2O @25 °C): 6.8 - 7.2
  • Microorganism (E. coli ATCC 23724): Good to Excellent
  • Microorganism (E. coli ATCC 33694): Good to Excellent
  • Microorganism (E. coli ATCC 33849): Good to Excellent
  • Microorganism (E. coli ATCC 39403): Good to Excellent
  • Microorganism (E. coli ATCC 47014): Good to Excellent
  • BSE/TSE Free: BSE/TSE Free
  • Country of Origin : USA
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