Molecular Biology Reagents/Chemicals

MIDSCI gives you ultra-pure, cost-competitive molecular biology reagents and lab chemicals for life science research; often with significant cost savings over Sigma Aldrich and other lab reagent providers.

PR1MA Chemicals can help with a wide variety of research-based requirements.
 
Use our glycerol for polyacrylamide gel electrophoresis. Eliminate Agrobacterium after inoculation with the carbenicillin we carry. Or detect β-Galactosidase activity with X-GAL which, when combined with the IPTG we stock, can be used to detect lac gene expression in cloning applications.
 
We also carry Luria Broth (Lennox), for the propagation and maintenance of E. coli; DTT, in order to maintain –SH groups in a reduced state; and high gel strength agar for tissue culture and microbiological work.

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PR1MA Urea, UltraPure (USP Grade)

CAS Number: 57-13-6
Molecular Weight: 60.06
Chemical Formula: CH4N2O
Solubility: Water
Storage Temperature: Room Temperature


Research or further manufacturing use only, not for food or drug use.


  • Appearance: White Crystalline Powder
  • Assay: 99.0 - 100.5 %
  • Identification (A, B): Pass Test
  • Appearance of 20% Solution: Pass Test
  • Melting Point: 132.5 to 134.5 oC
  • Moisture (LOD):  1.0 %
  • Alcohol- Insoluble Matter:  0.04 %
  • Residue on Ignition:  0.1 %
  • Chloride:  5 ppm
  • Sulfate:  0.001 %
  • Alkalinity: To pass test
  • Ammonium:  0.05 %
  • Urea Related Compound A:  0.1 %
  • Individual Unspecified Impurities:  0.1 %
  • Total impurities:  2.0 %
  • Heavy Metals:  0.001 %
  • Iron:  0.001 %
  • Residual Solvents USP 467: Meets requirements
  • BSE/TSE Free: BSE/TSE Free
  • Grade: USP
  • Country of Origin : Report

Kappa is now PR1MA - Same great product, new name
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PR1MA  Validamycin A

CAS Number: 37248-47-8
Molecular Weight: 497.5
Chemical Formula: C20H35NO13
Solubility: DMSO, slightly soluble in ethanol
Storage Temperature: 2-8°C



Inhibits trehalase activity. Enhances trehalose accumulation in transgenic plants.



Research or further manufacturing use only, not for food or drug use.


  • Appearance: Light yellow loose powder
  • Assay:  64.0 %
  • pH: 2.5 - 5.0
  • Water:  5.0 %
  • Water Insoluble:  0.2 %

Kappa is now PR1MA - Same great product, new name
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PR1MA Water, HPLC Grade

CAS Number: 7732-18-5
Molecular Weight: 18.0
Chemical Formula: H2O
Storage Temperature: Room Temperature


Certified RNase and DNase free for use in molecular biology applications. Ideal for making reagents, rinsing glass and plastic ware, enzymatic reactions and more. Toxic agents such as DEPC are not used in purification procedures.

  • Endotoxin Free
  • RNase Free
  • DNase Free


*Tested to < 0.001 EU/ML


Research or further manufacturing use only, not for food or drug use.


  • Appearance: Clear and Colorless Solution
  • TOC (ppb):  3 ppb
  • UV Absorbance (1 cm cell Vs Water) @ 190nm:  0.01
  • UV Absorbance (1 cm cell Vs Water) @ 200 nm:  0.010
  • UV Absorbance (1 cm cell Vs Water) @ 210 nm:  0.010
  • UV Absorbance (1 cm cell Vs Water) @ 250 nm:  0.005
  • UV Absorbance (1 cm cell Vs Water) @ 254 nm:  0.005
  • UV Absorbance (1 cm cell Vs Water) @ 280 nm:  0.005
  • Filtered through Ultrafiltration (0.01 Micron): Conforms
  • Country of Origin: USA

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PR1MA  Water, Molecular Biology Grade, DNase and RNase Free

CAS Number: 7732-18-5
Molecular Weight: 18.0
Chemical Formula: H2O
Storage Temperature: Room Temperature


Certified RNase and DNase free for use in molecular biology applications. Ideal for making reagents, rinsing glass and plastic ware, enzymatic reactions and more. Toxic agents such as DEPC are not used in purification procedures.
 

  • Endotoxin Free
  • RNase Free
  • DNase Free


*Tested to < 0.001 EU/ML


Meets or exceeds CAP/CLSI specifications for Clinical Laboratory Reagent Water (CLRW) and USP/NF Purified Water.


Research or further manufacturing use only, not for food or drug use.
 

  • Appearance: Clear and Colorless Solution
  • Bioburden : No Growth
  • DNase: No Visible Degradation
  • Protease: No Visible Degradation
  • RNase: No Visible Degradation
  • Country of Origin: USA

[5-Bromo-4-chloro-3-indolyl--D-galactoside]

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PR1MA™ X-GAL [5-Bromo-4-chloro-3-indolyl-β-D-galactoside]

Common Name: 5-Bromo-4-chloro-3-indolyl-β-D-galactoside
CAS Number: 7240-90-6
Molecular Weight: 408.6
Chemical Formula: C14H15BrCINO6
Solubility: DMSO and DMF
Storage Temperature: -20°C


Chromogenic substrate of β-Galactosidase used in combination with IPTG for detection of β-Galactosidase activity in bacterial colonies. X-GAL is cleaved at the B1-4 bond between galactose and the 5-Bromo-4-chloro-3-indolyl portion of X-GAL by β-Galactosidase via hydrolysis. The cleavage of X-GAL results in the production of a water insoluble blue dichloro-dibromo-indigo precipitate. In cloning strategies with vectors such as Lambda-11, M13mp18 and 19, pUC18 and 19, pUR222 the E. coli lacZ gene is transformed to lac+ cells. After transformation the cells show β-Galactosidase activity in the presence of media containing X-GAL and IPTG. The insertion of a DNA fragment into the cloning sites of the lacZ gene results in the disruption of β-Galactosidase activity leading to the appearance of white colonies. Non-recombinant cells produce a blue indigo dye.


Water Content: 0.05% (K. Fisher)
Protect from light and moisture


Research or further manufacturing use only, not for food or drug use.


  • Appearance: White Powder
  • Identity: Conforms to structure
  • Purity (HPLC):  98.0 %
  • Solubility (2%, DMF): Clear and Colorless Solution
  • Specific Rotation: -63.0 - -60.0 °
  • Water Content (K.F):  0.5 %

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PR1MA Yeast Extract, Granulated

CAS Number: 8013-01-2
Solubility: Water
Storage Temperature: Room Temperature


Dried yeast autolysate with a high content of amino nitrogen and water soluble B complex vitamins. Material is specially granulated to reduce dust and increase solubility rate.


Suitable for Microbiology Applications.


Research or further manufacturing use only, not for food or drug use.


  • Appearance: Light Beige Powder
  • Amino Nitrogen Content:  3.5 %
  • Dry Matter:  94 %
  • Moisture :  6.0 %
  • pH (2.0% Solution @ 25 oC): 6.5 - 7.5
  • Sodium Chloride:  0.5 %
  • Total Nitrogen Content:  8.0 %
  • Non Animal Origin: The product does not come in contact with materials of animal origin.

Kappa is now PR1MA - Same great product, new name
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PR1MA Yeast Extract, Powder

CAS Number: 8013-01-2
Solubility: Water
Storage Temperature: Room Temperature


Dried yeast autolysate with a high content of amino nitrogen and water soluble B complex vitamins. Due to its low NaCl content this quality is well suited for tissue culture.


Ingredients:

  • Total Nitrogen (TN): ~9.8%
  • Amino Nitrogen (AN): ~5.1%
  • AN/TN Ratio: ~0.52
  • Sodium Chloride: ~0.3%
  • pH (1% Solution): ~7.0

Research or further manufacturing use only, not for food or drug use.


  • Appearance: Light Beige Powder
  • Amino Nitrogen: 4.5 - 5.8 %
  • Ash Content: 11.50 - 16.0 %
  • Dry Matter:  95.0 %
  • Loss on Drying:  5.0 %
  • pH (2.0% Solution @ 25 oC): 6.8 - 7.2
  • Sodium Chloride:  0.5 %
  • Total Nitrogen: 10.0 - 11.8 %
  • Clostridium Perfringens Spores : Report cfu/g
  • E.Coli: Report cfu/g
  • Molds and Yeasts: Report cfu/g
  • Pathenogenic Staphylococcus: Report cfu/g
  • Salmonella : Report cfu/25g
  • Standard Plate Count: Report cfu/g

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PR1MA Yeast Nitrogen Base without Amino Acids

Solubility: Water
Storage Temperature: Room Temperature


For classifying yeasts based on amino acid and carbohydrates requirements. Contains all essential vitamins and inorganic salts necessary for the cultivaiton of yeasts. Excludes histidine methionine, tryptophan and carbon source. Also contains <1 mg/L each of vitamins and trace inorganic salts.


Research or further manufacturing use only, not for food or drug use.


  • DCM Appearance: Free flowing powder, Homogeneous, Off white to cream
  • PPM Appearance: Pale to light yellow (10X Solution)
  • pH (0.67% Solution @25 oC): 5.2 - 5.6
  • Microorganism Koleckera Apiculata ATCC 9774 (With Additives): Good to Excellent
  • Microorganism Saccharomyces Cerevisiae ATCC 9080 (With Additives): Good to Excellent
  • Microorganism Saccharomyces Cerevisiae ATCC 9763 (With Additives): Good to Excellent
  • Microorganisms Candida Albicans ATCC 10231 (With Additives): Good to Excellent

Electrophoresis Buffers
QuickSilver - TAE (Tris-acetate-EDTA)

TAE is ideal for separation of large DNA fragments in agarose gels and is used as running buffer and in gel preparation.

TAE is the buffer of choice when recovering DNA from gels and when downstream processing involves enzymatic reactions. Final concentration of a 1X working solution is 40mM Tris, 20mM acetic acid, 1mM EDTA.

QS1000 QuickSilver TAE Buffer, 1X, 100 packs

 

QuickSilver - TBE (Tris-borate-EDTA)

TBE is used in gel preparation and as a running buffer for electrophoresis of nucleic acids. TBE works best for resolution of smaller fragments (<2kb). Its higher buffering capacity makes it useful for longer runs. The final concentration of a 1X working solution is 89mM Tris, 89mM boric acid, 2mM EDTA.

QS1001 QuickSilver TBE Buffer, 1X, 50 packs

 

QuickSilver - Fast Running Buffer

QuickSilver Fast Running Buffer is a proprietary blend formulated for agarose gel electrophoresis at high voltage (300-350V). Its strong buffering capacity minimizes heat generation all allows for extremely fast separation of fragments smaller than 5kb. A typical 30-minute run can be performed in just 10 minutes using this buffer.

QS1002 QuickSilver Fast Running Buffer, 1X, 100 packs
 
 

Protein Electrophoresis Buffers

QuickSilver™ TGS (Tris-Glycine-SDS)

Tris-Glycine-SDS is one of the most common running buffers used for SDS-PAGE. A 1X solution contains 25mM Tris, 192mM Glycine and 0.1% SDS.

QS1100 QuickSilver Tris Glycine-SDS Buffer, 1X, 50 packs


QuickSilver™ Tris-MES-SDS

Small to medium molecular weight proteins can be resolved on SDS-PAGE gels using Tris-MES-SDS as a running buffer. Proteins resolve faster in gels using Tris-MES-SDS as a running buffer than those using Tris-MOPS-SDS. A 1X solution has a final concentration of 50mM Tris, 50mM MES, 0.1% SDS and 1mM EDTA.

QS1101 QuickSilver Tris-MES-SDS Buffer, 1X, 50 packs


QuickSilver™ Tris-MOPS-SDS

Tris-MOPS-SDS is ideal for use as a running buffer when resolving medium to large proteins with SDS-PAGE. Gels run with Tris-MOPS-SDS buffer run slower than those using Tris-MES-SDS buffer. The final concentration of a 1X working solution is 50mM Tris, 50mM MOPS, 0.1% SDS and 1mM EDTA.

QS1102 QuickSilver Tris-MOPS-SDS, 1X, 50 packs



Western Blotting, ELISA, Cell Culture, Chromatography

QuickSilver™ PBS and PBST

Because its osmolality and ion concentration closely mimic that of the human body, PBS is commonly used for cell culture applications, as well as HPLC and MS. PBST is a wash buffer and diluent for ELISA. The 1X PBS solution contains 137mN NaCl, 2.7mM KCl and 10mM phosphate. PBST is supplemented with 0.05% Tween-20.

QS1200 QuickSilver PBS Buffer, 1X, 100 packs

QS1201 QuickSilver PBST Buffer, 1X, 100 packs


QuickSilver™ TBS and TBST

TBS and TBST are used to maintain pH within a very narrow range for a variety of applications including western blotting and immunoassays. The final concentration of a 1X working solution is 137mM NaCl, 2.7mM KCl, 50mM Tris. In addition, 1X TBST contains 0.1% Tween-20.

QS1202 QuickSilver TBS buffer, 1X, 100 packs

QS1203 QuickSilver TBST buffer, 1X, 100 packs


Item#:
ASQUICKSILVER
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