Molecular Biology Reagents/Chemicals

MIDSCI™ gives you ultra-pure, cost-competitive molecular biology reagents and lab chemicals for life science research; often with significant cost savings over Sigma Aldrich and other lab reagent providers.

PR1MA™ Chemicals can help with a wide variety of research-based requirements.
 
Use our glycerol for polyacrylamide gel electrophoresis. Eliminate Agrobacterium after inoculation with the carbenicillin we carry. Or detect β-Galactosidase activity with X-GAL which, when combined with the IPTG we stock, can be used to detect lac gene expression in cloning applications.
 
We also carry Luria Broth (Lennox), for the propagation and maintenance of E. coli; DTT, in order to maintain –SH groups in a reduced state; and high gel strength agar for tissue culture and microbiological work.

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PR1MA  Timentin [Ticarcillin Disodium Salt/Potassium Clavulanate Mixture 15:1 Ratio]

Common Name: Ticarcillin Disodium Salt/Potassium Clavulanate Mixture 15:1 Ratio
CAS Number: 4697-14-7; 61177-45-5
Molecular Weight: 428.39 / 665.7
Chemical Formula: C15H14N2Na2O 6S2 / C8H8KNO5
Solubility: Water
Storage Temperature: 2-8°C



Highly effective combination against gram negative bacteria such as Agrobacterium strains. -Lactamase inhibitor.


Plant cell culture tested. Hygroscopic.


Mixture Contains:

  • 1875 mg Ticarcillin to 125 mg potassium clavulanate (15:1 ratio)


Research or further manufacturing use only, not for food or drug use.
 
 

  • Appearance: White to Pale Yellow Crystalline Powder
  • Assay (Clavulanic Acid):  5.0 %
  • Assay (Ticarcillin):  75.0 %
  • Bacterial Endotoxins :  0.07 EU/mg
  • Identification: Conform
  • Particulate Matter: Conforms
  • pH: 5.5 - 7.5
  • Potency (Clavulanic Acid):  50 µg/mg
  • Potency Ticarcillin:  750 µg/mg
  • Proportion of Ticarcillin to Clavulanic acid: 14:1 - 16:1
  • Related Substances (Largest Impurity):  4.0 %
  • Related Substances (Second Major Impurity) :  2.5 %
  • Water (KF):  4.5 %

PR1MA™ Miller's LB Agar Capsules


Common Name:
Miller's LB Agar

Solubility:
Water with Heat

Storage Temperature:
Room Temperature


Super convenient. Eliminate messy weighing. Consistant and accurate measurement. Convenient premeasured and pre-mixed capsules. Prebuffered media eliminates pH adjustments.

Simply add 40 capsules to one liter of water, sterilize by autoclaving for 15 minutes at 121°C. Capsules contain all necessary ingredients for maintenance and propagation of E. Coli.

Ingredients:

Casein Digest Peptone: 10g/L
Yeast Extract: 5g/L
Sodium Chloride: 10g/L
Agar:15g/L
TRIS/TRIS HCl: 1.5g/L
Item#:
ASPR1MAMILLERLBAGAR

PR1MA™ Temed

 

Common Name: N,N,N',N'-Tetramethylethylenediamine

CAS Number: 110-18-9

Molecular Weight: 116.2

Chemical Formula: C6H16N2

Solubility: Water

Storage Temperature: Room Temperature

 

Tertiary amine used to catalyze formation of free radicals from ammonium persulfate and riboflavin. Suitable for electrophoresis and tested as a catalyst for polyacrylamide gel electrophoresis.

 

Shipping Weight: 0.4 lbs

Shipping Dimensions: 2.00 x 4.50 x 2.00

Research or further manufacturing use only, not for food or drug use.

 

 

Specifications

 

Appearance: Clear, Colorless Solution

Identification: Conforms

Purity (GC): ≥ 97 %

BSE/TSE Free: BSE/TSE Free

Country of Origin: Report

 

 

[5-Bromo-4-chloro-3-indolyl-β-D-galactoside]

PR1MA™ X-GAL [5-Bromo-4-chloro-3-indolyl-β-D-galactoside]

Common Name: 5-Bromo-4-chloro-3-indolyl-β-D-galactoside
CAS Number: 7240-90-6
Molecular Weight: 408.6
Chemical Formula: C14H15BrCINO6
Solubility: DMSO and DMF
Storage Temperature: -20°C


Chromogenic substrate of β-Galactosidase used in combination with IPTG for detection of β-Galactosidase activity in bacterial colonies. X-GAL is cleaved at the B1-4 bond between galactose and the 5-Bromo-4-chloro-3-indolyl portion of X-GAL by β-Galactosidase via hydrolysis. The cleavage of X-GAL results in the production of a water insoluble blue dichloro-dibromo-indigo precipitate. In cloning strategies with vectors such as Lambda-11, M13mp18 and 19, pUC18 and 19, pUR222 the E. coli lacZ gene is transformed to lac+ cells. After transformation the cells show β-Galactosidase activity in the presence of media containing X-GAL and IPTG. The insertion of a DNA fragment into the cloning sites of the lacZ gene results in the disruption of β-Galactosidase activity leading to the appearance of white colonies. Non-recombinant cells produce a blue indigo dye.


Water Content: 0.05% (K. Fisher)
Protect from light and moisture


Research or further manufacturing use only, not for food or drug use.


  • Appearance: White Powder
  • Identity: Conforms to structure
  • Purity (HPLC):  98.0 %
  • Solubility (2%, DMF): Clear and Colorless Solution
  • Specific Rotation: -63.0 - -60.0 °
  • Water Content (K.F):  0.5 %

PR1MA  Mitomycin C

CAS Number: 50-07-7
Molecular Weight: 334.3
Chemical Formula: C15H18N4O5
Solubility: Ethanol
Storage Temperature: 2-8°C
 

Antineoplastic antibiotic that acts as an alkylating agent after activation and also suppresses the synthesis of nucleic acids. A cell-cycle non specific agent, most active in the late G1 and early S phases.


WARNING! This product can expose you to Mitomycin-C, a chemical which is known to the State of California to cause cancer. For more information go to www.P65Warnings.ca.gov.


Research or further manufacturing use only, not for food or drug use.
 
 

  • Appearance: Blue violet solid
  • Identification (UV): Conforms
  • Purity-HPLC (RP CH3CN:H2O-0-100%):  99.0 %
  • Purity-HPLC (RP MEOH:H2O-90-10):  98.0 %
  • Purity-TLC (CH2CL2:MEOH- 9:1):  98.0 %
  • Solubility (10 mg/ml MEOH): Clear Blue Solution

PR1MA  MOPS [3-(N-morpholino)-propanesulfonic acid]

Common Name: 3-(N-Morpholino)propanesulfonic acid
CAS Number: 1132-61-2
Molecular Weight: 209.3
Chemical Formula: C7H15NO4S
Solubility: Water
Storage Temperature: Room Temperature


Molecular Biology Grade

  • Heavy Metals: <5 ppm
  • pKa: (20°C) 7.20 ± 0.2
  • pH Range: 6.5-7.9

Research or further manufacturing use only, not for food or drug use.
 

  • Appearance: White crystalline powder
  • Absorbance @250nm, 1M solution in H2O:  0.1
  • Absorbance 260nm:  0.2 nm
  • Absorbance 280nm:  0.2
  • Assay (Titration):  99.0 %
  • Endotoxin :  0.5 EU/mg
  • Heavy Metals :  5 ppm
  • Identification: Matches the reference
  • Iron (Fe):  5 ppm
  • Loss on Drying (105 oC):  1.0 %
  • Melting Point : 276.0 - 281.0 oC
  • pH: 2.5 - 5.0
  • pKa @ 20°C: 7.0 - 7.4
  • Residue on Ignition:  0.2 %
  • Solubility: Clear, colorless solution

PR1MA Acrylamide, 40% Solution

CAS Number: 79-06-1; 7732-18-5
Storage Temperature: 2-8°C


A 40% (w/v) Acrylamide solution.


WARNING! This product can expose you to Acrylamide, a chemical which is known to the State of California to cause cancer, developmental and reproductive harm. For more information go to www.P65Warnings.ca.gov.


Research or further manufacturing use only, not for food or drug use.


  • Appearance: Clear, Colorless Solution
  • Acrylic Acid:  0.003 %
  • Conductivity:  5 µmhos
  • DNase, RNase, protease: Not Detected
  • Electrophoresis: Pass Test
  • pH @25 °C: 6.0 - 7.0

Bullseye Agarose General Purpose

 

Bullseye Molecular Biology Grade Agarose is certified for use in standard electrophoresis procedures, including nucleic acid analysis, preparative electrophoresis, and blotting. This highly refined and purified agarose is produced under strict testing parameters to ensure lot-to-lot consistency. It features low electrophoretic mobility characteristics, making it ideal for a variety of applications.

 

Extracted from the cell walls of certain Rhodophyceae (agarophyte seaweeds), Bullseye Standard Agarose is a neutral polysaccharide composed of galactan, linked by agarobioses in a 1-3, 1-4 arrangement. This unique chemical structure enables agarose to form robust gels even at low concentrations. The gels exhibit a macroreticular structure with an open mesh, easily adjustable by varying the agarose concentration.

 

Bullseye Agarose GP2 offers:

  • General-purpose electrophoresis and blotting capabilities
  • Excellent separation, resolution, and clarity
  • Low absorption and background from staining reagents
  • High gel strength for safe and easy handling
  • Superior refinement and purification
  • Enhanced thermal stability
  • Comprehensive packaging including a specification sheet, preparation instructions, and MSDS
  • Choose Bullseye Standard Agarose for reliable performance in your molecular biology applications.

 

 

Physical Specifications:

CAS#: 9012-36-6
Moisture: ≤ 7%
Ash: ≤ 0.4%
EEO: 0.05-0.13
Sulfate: ≤ 0.10%
Clarity @ 1.5% (NTU): ≤ 3
Gel Strength @ 1.0% (g/cm2): ≥ 1200
Gel Strength @ 1.5% (g/cm2): ≥ 2500
Gelling Temperature @ 1.5%: 36°C ± 1.5°C
Melting Temperature @ 1.5%: 88°C ± 1.5°C

 

 

Molecular Biology Specifications:

DNase assay: None Detected
RNase assay: None Detected
Protease assay: None Detected
Gel analysis assay: Pass
DNA Resolution: ≥ 1000bp (Finely Resolved)
DNA Extraction assay: Pass
Gel Background: Very Low

 

 

Base Pair Data
1X TAE BufferGel Concentration %1X TBE Buffer
50-1500bp250-1,000bp
100-2500bp1.75100-2,000bp
200-5000bp1.5150-3,000bp
250-10,000bp1.25200-4,000bp
300-16,000bp1300-8,000bp
500-20,000bp0.75500-12,000bp
Item#:
ASPCRAGAROSE1

PR1MA™ Potassium Phosphate, Monobasic, ACS

 

CAS Number: 7778-77-0
Molecular Weight: 136.1
Chemical Formula: KH2PO4
Solubility: Water
Storage Temperature: Room Temperature

 

Research or further manufacturing use only, not for food or drug use.

 
 

  • Appearance: White crystalline powder
  • Odor: Odorless
  • pH (5% Solution): 4.1 - 4.5
  • Insoluble Matter: ≤ 0.01 %
  • Loss on Drying: ≤ 0.2 %
  • Chloride (Cl): ≤ 0.001 %
  • Sulfate: ≤ 0.003 %
  • Heavy Metals: ≤ 0.001 %
  • Iron: ≤ 0.002 %
  • Sodium (Na): ≤ 0.005 %
  • Assay: ≥ 99.0 %
  • Grade: ACS
  • BSE/TSE Free: BSE/TSE Free
  • Non-Animal Origin: Non-Animal Origin
  • Country of Origin: Report

 
 

PR1MA  Cefsulodin, Sodium Salt

CAS Number: 52152-93-9
Molecular Weight: 554.5
Chemical Formula: C22H19N4NaO8S 2
Solubility: Water
Storage Temperature: 2-8°C


Cefsulodin is a third generation cephalosporin antibiotic that inhibits cell wall synthesis. It has high activity against Pseudomonas aeruginosa with no significant activity against other gram negative bacteria. Limited activity against gram positive and anaerobic bacteria.


Research or further manufacturing use only, not for food or drug use.
 

  • Appearance: White to yellow crystalline powder
  • Assay (Anhydrous):  86.0 %
  • Chromatographic Purity :  98.0 %
  • Isonicotinic Acid Amide:  1.0 %
  • pH: 3.3 - 4.8
  • Specific Optical Rotation: +16.5 - +20.0 °
  • Total of other impurities:  1.5 %
  • Water:  5.0 %

Agar, Bacteriological Grade

 

CAS Number: 9002-18-0

Solubility: Water with Heat

Storage Temperature: Room Temperature

 

Mix 15 grams per liter in Molecular Biology grade water.

Shipping Weight: 1.4 lbs

Shipping Dimensions: 3.50 x 7.00 x 3.50


Research or further manufacturing use only, not for food or drug use.

 

Appearance: White to Cream Powder

Gel Strength (1.5% gel, 20°C): 600 - 1100 g / cm³

Loss on Drying: Report %

Item#:
ASAGARBACGR

PR1MA™ Sodium Carbonate Anhydrous, ACS Grade

Common Name: Soda Ash
CAS Number: 497-19-8
Molecular Weight: 105.99
Chemical Formula: Na2CO3
Solubility: Water
Storage Temperature: Room Temperature


Research or further manufacturing use only, not for food or drug use.


  • Appearance: Fine White Granular Powder
  • Calcium (Ca):  0.03 %
  • Chloride (Cl):  0.001 %
  • Heavy Metals:  0.0005 %
  • Insolubles:  0.01 %
  • Iron:  0.0005 %
  • Loss On Heating:  1.0 %
  • Magnesium (Mg):  0.005 %
  • Phosphate:  0.001 %
  • Potassium (K):  0.005 %
  • Purity:  99.5 %
  • Silica:  0.005 %
  • Sulfur Compound:  0.003 %
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