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ActionsMolecular Biology Reagents/Chemicals
MIDSCI™ gives you ultra-pure, cost-competitive molecular biology reagents and lab chemicals for life science research; often with significant cost savings over Sigma Aldrich and other lab reagent providers.
PR1MA™ Chemicals can help with a wide variety of research-based requirements.
Use our glycerol for polyacrylamide gel electrophoresis. Eliminate Agrobacterium after inoculation with the carbenicillin we carry. Or detect β-Galactosidase activity with X-GAL which, when combined with the IPTG we stock, can be used to detect lac gene expression in cloning applications.
We also carry Luria Broth (Lennox), for the propagation and maintenance of E. coli; DTT, in order to maintain –SH groups in a reduced state; and high gel strength agar for tissue culture and microbiological work.
PR1MA™ Trimethoprim
CAS Number: 738-70-5
Molecular Weight: 290.3
Chemical Formula: C14H18N4O3
Solubility: DMSO 50mg/ml, Methanol 12:1 mg/ml, Water 0.4 mg/ml
Storage Temperature: 2-8°C
Inhibits dihydrofolate reductase. Active against most gram negative and
gram positive aerobic bacteria.
Research or further manufacturing use only, not for food or drug use.
- Appearance: Off White to Light Yellow Crystalline Powder
- Assay (Anhydrous): 98.5 - 101 %
- Heavy Metals : ≤ 20 ppm
- Loss on Drying: ≤ 1.0 %
- Melting Point: 199 - 203 oC
- Related Substances: ≤ 0.2 %
- Sulfated Ash: ≤ 0.1 %
PR1MA™ SOB Broth
Solubility: Water
Storage Temperature: Room Temperature
SOB Broth is recommended for use in cultivation of recombinant strains of
E. Coli. It is a nutritionally rich growth medium for use in the
preparation and transformation of competent cells.
Add 28 grams of Broth in 1 liter of distilled water. Heat gently to
dissolve the medium completely. Dispense in tubes and sterilize by
autoclaving for 15 minutes.
Ingredients:
- Casein Enzymic Hydrolysate: 20 g/L
- Magnesium Sulfate: 2.4 g/L
- Yeast Extract: 5 g/L
- Potassium Chloride: 0.186 g/L
- Sodium Chloride: 0.5 g/L
- Final pH(25°:C): 7.0+0.2
Research or further manufacturing use only, not for food or drug use.
- DCM Appearance: Homogeneous Free Flowing,Light Beige
- PPM Appearance: Clear, no to light ppt, light yellow
- pH (2.8 % Solution @25 oC): 6.8 - 7.2
- Microorganism (E. coli ATCC 23724): Good to Excellent
- Microorganism (E. coli ATCC 33694): Good to Excellent
- Microorganism (E. coli ATCC 33849): Good to Excellent
- Microorganism (E. coli ATCC 39403): Good to Excellent
- Microorganism (E. coli ATCC 47014): Good to Excellent
- Microorganism (E. coli ATCC 53868): Good to Excellent
PR1MA™ Tween 20
Common Name:
Polyoxyethylenesorbitan Monolaurate
CAS Number:
9005-64-5
Solubility:
Water
Storage Temperature:
Room Temperature
Fatty acid composition:
- Lauric acid: Approximately 50%
- Balance: Myristic, Palmitic and Stearic acids.
Shipping Weight: 1.2 lbs
Shipping Dimensions: 2.00 x 6.00 x 2.00
Specifications
Appearance: Yellow to amber viscous liquid
1,4-Dioxane: ≤ 10 ppm
Acid Value: ≤ 2.20 mg KOH/g
Color, Gardner: ≤ 5.0
Hydroxyl Value: 96 - 108 mgKOH/g
Saponification Value: 40.0 - 50.0 mg KOH/g
Water: ≤ 3.00 %
Research or further manufacturing use only, not for food or drug use.
PR1MA™ Sulfamethoxazole
CAS Number: 723-46-6
Molecular Weight: 253.3
Chemical Formula: C10H11N3O3S
Solubility: Acetone
Storage Temperature: Room Temperature
Bacteriostatic antibiotic that inhibits nucleic acid synthesis in sensitive micro-organisms.
Research or further manufacturing use only, not for food or drug use.
- Appearance: White to Off White Crystalline Powder
- Infrared Spectrum: Conforms to Structure
- Loss on Drying: ≤ 0.5 %
- Residue on Ignition: ≤ 0.1 %
- Related Substance (Sulfanilic acid): ≤ 0.10 %
- Related Substance (Sulfanilamide): ≤ 0.10 %
- Related Substance (Impurity C, A): ≤ 0.10 %
- Related Substance (Impurity B): ≤ 0.10 %
- Related Substance (Impurity F): ≤ 0.10 %
- Any other Impurity: ≤ 0.10 %
- Total impurities: ≤ 0.30 %
- Assay (Titration): 99.0 - 101.0 %
- Grade: USP
- Country of Origin: Report
PR1MA™ Validamycin A
CAS Number: 37248-47-8
Molecular Weight: 497.5
Chemical Formula: C20H35NO13
Solubility: DMSO, slightly soluble in ethanol
Storage Temperature: 2-8°C
Inhibits trehalase activity. Enhances trehalose accumulation in transgenic plants.
Research or further manufacturing use only, not for food or drug use.
- Appearance: Light yellow loose powder
- Assay: ≥ 64.0 %
- pH: 2.5 - 5.0
- Water: ≤ 5.0 %
- Water Insoluble: ≤ 0.2 %
PR1MA™ Timentin [Ticarcillin Disodium Salt/Potassium Clavulanate Mixture 15:1 Ratio]
Common Name: Ticarcillin Disodium Salt/Potassium Clavulanate Mixture 15:1
Ratio
CAS Number: 4697-14-7; 61177-45-5
Molecular Weight: 428.39 / 665.7
Chemical Formula: C15H14N2Na2O 6S2 / C8H8KNO5
Solubility: Water
Storage Temperature: 2-8°C
Highly effective combination against gram negative bacteria such as
Agrobacterium strains. -Lactamase inhibitor.
Plant cell culture tested. Hygroscopic.
Mixture Contains:
- 1875 mg Ticarcillin to 125 mg potassium clavulanate (15:1 ratio)
- Appearance: White to Pale Yellow Crystalline Powder
- Assay (Clavulanic Acid): ≥ 5.0 %
- Assay (Ticarcillin): ≥ 75.0 %
- Bacterial Endotoxins : ≤ 0.07 EU/mg
- Identification: Conform
- Particulate Matter: Conforms
- pH: 5.5 - 7.5
- Potency (Clavulanic Acid): ≥ 50 µg/mg
- Potency Ticarcillin: ≥ 750 µg/mg
- Proportion of Ticarcillin to Clavulanic acid: 14:1 - 16:1
- Related Substances (Largest Impurity): ≤ 4.0 %
- Related Substances (Second Major Impurity) : ≤ 2.5 %
- Water (KF): ≤ 4.5 %
PR1MA™ Miller's LB Agar Capsules
PR1MA™ X-GAL [5-Bromo-4-chloro-3-indolyl-β-D-galactoside]
Common Name: 5-Bromo-4-chloro-3-indolyl-β-D-galactoside
CAS Number: 7240-90-6
Molecular Weight: 408.6
Chemical Formula: C14H15BrCINO6
Solubility: DMSO and DMF
Storage Temperature: -20°C
Chromogenic substrate of β-Galactosidase used in combination with IPTG for
detection of β-Galactosidase activity in bacterial colonies. X-GAL is
cleaved at the B1-4 bond between galactose and the
5-Bromo-4-chloro-3-indolyl portion of X-GAL by β-Galactosidase via
hydrolysis. The cleavage of X-GAL results in the production of a water
insoluble blue dichloro-dibromo-indigo precipitate. In cloning strategies
with vectors such as Lambda-11, M13mp18 and 19, pUC18 and 19, pUR222 the E.
coli lacZ gene is transformed to lac+ cells. After transformation the cells
show β-Galactosidase activity in the presence of media containing X-GAL and
IPTG. The insertion of a DNA fragment into the cloning sites of the lacZ
gene results in the disruption of β-Galactosidase activity leading to the
appearance of white colonies. Non-recombinant cells produce a blue indigo
dye.
Water Content: 0.05% (K. Fisher)
Protect from light and moisture
Research or further manufacturing use only, not for food or drug use.
- Appearance: White Powder
- Identity: Conforms to structure
- Purity (HPLC): ≥ 98.0 %
- Solubility (2%, DMF): Clear and Colorless Solution
- Specific Rotation: -63.0 - -60.0 °
- Water Content (K.F): ≤ 0.5 %
PR1MA™ Mitomycin C
CAS Number: 50-07-7
Molecular Weight: 334.3
Chemical Formula: C15H18N4O5
Solubility: Ethanol
Storage Temperature: 2-8°C
Antineoplastic antibiotic that acts as an alkylating agent after activation
and also suppresses the synthesis of nucleic acids. A cell-cycle non
specific agent, most active in the late G1 and early S phases.
WARNING! This product can expose you to Mitomycin-C, a chemical which
is known to the State of California to cause cancer. For more
information go to www.P65Warnings.ca.gov.
Research or further manufacturing use only, not for food or drug use.
- Appearance: Blue violet solid
- Identification (UV): Conforms
- Purity-HPLC (RP CH3CN:H2O-0-100%): ≥ 99.0 %
- Purity-HPLC (RP MEOH:H2O-90-10): ≥ 98.0 %
- Purity-TLC (CH2CL2:MEOH- 9:1): ≥ 98.0 %
- Solubility (10 mg/ml MEOH): Clear Blue Solution
PR1MA™ MOPS [3-(N-morpholino)-propanesulfonic acid]
Common Name: 3-(N-Morpholino)propanesulfonic acid
CAS Number: 1132-61-2
Molecular Weight: 209.3
Chemical Formula: C7H15NO4S
Solubility: Water
Storage Temperature: Room Temperature
Molecular Biology Grade
- Heavy Metals: <5 ppm
- pKa: (20°C) 7.20 ± 0.2
- pH Range: 6.5-7.9
Research or further manufacturing use only, not for food or drug use.
- Appearance: White crystalline powder
- Absorbance @250nm, 1M solution in H2O: ≤ 0.1
- Absorbance 260nm: ≤ 0.2 nm
- Absorbance 280nm: ≤ 0.2
- Assay (Titration): ≥ 99.0 %
- Endotoxin : ≤ 0.5 EU/mg
- Heavy Metals : ≤ 5 ppm
- Identification: Matches the reference
- Iron (Fe): ≤ 5 ppm
- Loss on Drying (105 oC): ≤ 1.0 %
- Melting Point : 276.0 - 281.0 oC
- pH: 2.5 - 5.0
- pKa @ 20°C: 7.0 - 7.4
- Residue on Ignition: ≤ 0.2 %
- Solubility: Clear, colorless solution
PR1MA™ Acrylamide, 40% Solution
CAS Number: 79-06-1; 7732-18-5
Storage Temperature: 2-8°C
A 40% (w/v) Acrylamide solution.
WARNING! This product can expose you to Acrylamide, a chemical which is
known to the State of California to cause cancer, developmental and
reproductive harm. For more information go to www.P65Warnings.ca.gov.
Research or further manufacturing use only, not for food or drug use.
- Appearance: Clear, Colorless Solution
- Acrylic Acid: ≤ 0.003 %
- Conductivity: ≤ 5 µmhos
- DNase, RNase, protease: Not Detected
- Electrophoresis: Pass Test
- pH @25 °C: 6.0 - 7.0
Click here for Agarose with a better price and ready to ship today!
Bulls Eye Agarose GP2 is highly refined and highly purified. Each lot is physically tested and verified for performance in its designated application.
Reasons why Bulls Eye Agarose GP2 will work for you:
1) Obviously Bulls Eye Agarose GP2 gives you everything you want in a High Quality General Purpose Agarose at a fair price.
2) Bulls Eye Agarose GP2 is produced to deliver lot-to-lot consistency
3) Quality and Purity
4) Packaged in 500g Bottles Bulls Eye Agarose saves the lab money without sacrificing quality.
Bulls Eye Agarose GP2 offers you:
- General purpose electrophoresis and blotting
- Excellent separation, resolution, and clarity
- Low absorption and background from staining reagents
- High gel strength for safe and easy handling
- Highly refined and purified
- Greater thermal stability
- Packaged with spec sheet, prep instructions, and MSDS
Physical Specifications: |
|
CAS# |
9012-36-6 |
Moisture |
7% |
Ash |
4.5% |
Sulfate |
0.1% |
Clarity @ 1.5% (NTU) |
4 |
Gel Strength @1.0% (g/cm2) |
1000 |
Gel Strength @1.5% (g/cm2) |
2200 |
Gelling Temperature @ 1.5% |
36°C ± 1.5°C |
Melting Temperature @ 1.5% |
88°C ± 1.5°C |
Base Pair Data |
||
1X TAE Buffer |
Gel Concentration % |
1X TBE Buffer |
50-1500bp |
2.0 |
50-1000bp |
100-2500bp |
1.75 |
100-2000bp |
200-5000bp |
1.5 |
150-3000bp |
250-10,000bp |
1.25 |
200-4000bp |
300-16,000bp |
1.0 |
300-8000bp |
500-20,000bp |
0.75 |
500-12,000bp |