Molecular Biology Reagents/Chemicals

MIDSCI™ gives you ultra-pure, cost-competitive molecular biology reagents and lab chemicals for life science research; often with significant cost savings over Sigma Aldrich and other lab reagent providers.

PR1MA™ Chemicals can help with a wide variety of research-based requirements.
 
Use our glycerol for polyacrylamide gel electrophoresis. Eliminate Agrobacterium after inoculation with the carbenicillin we carry. Or detect β-Galactosidase activity with X-GAL which, when combined with the IPTG we stock, can be used to detect lac gene expression in cloning applications.
 
We also carry Luria Broth (Lennox), for the propagation and maintenance of E. coli; DTT, in order to maintain –SH groups in a reduced state; and high gel strength agar for tissue culture and microbiological work.

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Limited Time Promo!

Benchmark Black Friday 2024 Promo!
For a limited time, buy ONE pack of EZ Pack Agarose Tablets (A2501) and get ONE FREE!


See flyer for more details.

 

Promo is valid from 11/04/24 - 12/06/24

All promotions are valid while supplies last. Prices are subject to change without notice.

Not to be combined with any offers or discounts. No institutional pricing. All terms apply.

Use item number +"-VF" or promo code TURKEY24
 

EZ Pack Agarose Tablets

  • Eliminates weighing
  • Fast dissolving, just two minutes
  • Environmentally friendly, free from organic solvents
  • Consistent, reproducible gels
  • Enhanced resolution and clarity 

Researchers trust Benchmark’s Agarose LE to meet their electrophoretic needs for various applications. Now, the same agarose is available in a convenient, no-mess tablet.


Agarose LE is refined in an advanced process that excludes the use of organic solvents. The result is a cleaner end product with significantly reduced environmental impact. The agarose can be used for analyses of nucleic acids from 150 bp to 25,000 bp, protein electrophoresis, and various blotting protocols.


The low EEO of the agarose promotes increased electrophoretic mobility, yielding improved resolution and shorter run times. This also allows macromolecules and larger particles (subcellular fragments, viruses, etc.) to migrate more freely through the gel matrix. The consistently low EEO reduces band distortion (caused by counterflow) that can result from the presence of excessive sulfate-rich negative ions. The quick-dissolving EZ Pack™ tablet contains 0.5 g (500 mg) of Agarose LE, eliminating the hands-on time and inaccuracies normally associated with weighing. Use of the tablet is not only convenient and cleaner, but it also provides better consistency and reproducibility gel to gel.

 

Gel preparation is simple – add the desired number of tablets to the electrophoresis buffer, allow it to sit for two minutes, and then heat and pour as usual. The resulting gels are highly transparent, have exceptional thermal stability (ensuring safe and easy handling), and exhibit exceptionally low absorption of chemical staining agents.

EZ Pack™ Agarose Tablets are supplied in convenient blister packs for safe, clean dispensing.

 

Specifications
 

Gel Temperature36°C ± 1.5°C (1.5%)
Melt Temperature88°C ± 1.5°C (1.5%)
EEO (-mr)< 0.13
Moisture Content< 10%
Sulfate< 0.2%
RNase/DNaseNone Detected
Protease/EndonucleaseNone Detected
Storage ConditionsRoom Temperature
Gel Strength> 1200 g / cm2 (1%)
> 2500 g / cm2 (1.5%)
 
Buffer volume required to achieve desired gel strength
GEL %0.8%1.0%1.2%1.3%1.5%1.8%2.0%
1 Tab63 mL50 mL42 mL38 mL33 mL28 mL25 mL
2 Tabs125 mL100 mL83 mL77 mL67 mL56 mL50 mL
3 Tabs188 mL150 mL125 mL115 mL100 mL83 mL75 mL

Item#:
ASAGROTBLTS

Kappa is now PR1MA - Same great product, new name
Looking for something else? Email tech@midsci.com. If we can get it, we will add it to the website.

 

PR1MA Agarose, Low Melt Temperature

CAS Number: 9012-36-6
Solubility: Water
Storage Temperature: Room Temperature


Ideal gelling properties for use in cloning assays or for recovery of heat labile samples following electrophoresis.


Gel Strength, 1%: >200 g/cm2
Gelling Temperature: 26-30°C
Melting Temperature: <65°C
Sulfate: <0.10%
EEO: <0.10
DNase and RNase: None Detected


Research or further manufacturing use only, not for food or drug use.
 

  • Appearance: White to off white powder
  • DNase/ RNase: Not Detected
  • EEO:  0.10 -Mr
  • Gelling Temperature: 26 - 30 oC
  • Gel Strength :  200 g/cm2
  • Melting Temperature:  65 oC
  • Moisture Content:  10 %
  • Sulfate Content:  0.10 %

PR1MA Terrific Broth Modified Capsules

CAS Number: 91079-40-2; 8013-01-2; 7778-77-0; 7758-11-4
Solubility: Water
Storage Temperature: Room Temperature


Terrific Broth formulation packaged in easy to use 24 gram capsules. Formulation from powder.


Add 1 capsule per 500ml of water. Heat and stir until capsules are completely dissolved. Add 2ml of glycerol to mixture. Sterilize by autoclaving for 15 minutes.


Ingredients:

  • Casein Digest Peptone: 12 g/L
  • Yeast Extract: 24 g/L
  • Dipotassium Phosphate: 9.4 g/L
  • Potassium Phosphate: 2.2 g/L



Research or further manufacturing use only, not for food or drug use.


  • Appearance: Water soluble gelatin capsules containing cream to beige free flowing powder.
  • Appearance of Solution: Prepared medium is light to medium amber and clear
  • pH (4.76% solution at 25°C): 7.0 - 7.4
  • Microorganism (E. coli ATCC 23724): Good to Excellent
  • Microorganism (E. coli ATCC 33694): Good to Excellent
  • Microorganism (E. coli ATCC 33849): Good to Excellent
  • Microorganism (E. coli ATCC 39403): Good to Excellent
  • Microorganism (E. coli ATCC 47014): Good to Excellent
  • Microorganism (E. coli ATCC 53868): Good to Excellent
  • Yeast Extract: 24.0 g/L
  • Casein Peptone: 12.0 g/L
  • Potassium phosphate dibasic: 9.4 g/L
  • Potassium phosphate monobasic: 2.2 g/L
  • Direction (Preparation): Add 2 capsules to one liter of purified water until evenly dispersed. Add 4ml of Glycerol to the mixture. Boil for one minute with stirring to completely dissolve. Distribute and autoclave at 121°C for 15 minutes.

 

PR1MA LB Broth 20 Gram Buffered Capsules [Lennox L Broth]

Common Name: Lennox L Broth
Solubility: Water
Storage Temperature: Room Temperature


RPI media is now available in convenient premeasured and pre-mixed capsules. Prebuffered media eliminates pH adjustments, dust and smells from the lab. Low Salt Formula. Molecular Biology Grade.


For propagation and maintenance of E.Coli:

  • Add 1 capsule per liter of water.
  • Heat and stir until capsule is completely dissolved.
  • Buffer is set for 7.2 at 20°C using a 10mM solution of TRIS/TRIS HCl
  • Sterilize by autoclaving for 15 minutes.



Ingredients:

  • Tryptone: 10 g/L
  • Yeast Extract: 5 g/L
  • Sodium Chloride: 5 g/L



Research or further manufacturing use only, not for food or drug use.


  • Appearance: Capsules
  • Appearance of Solution: Prepared medium is yellow to gold and clear to moderately hazy
  • Microorganism (E. coli ATCC 23724): Good to Excellent
  • Microorganism (E. coli ATCC 33694): Good to Excellent
  • Microorganism (E. coli ATCC 33849): Fair to Excellent
  • Microorganism (E. coli ATCC 39403): Good to Excellent
  • Microorganism (E. coli ATCC 47014): Good to Excellent
  • Microorganism (E. coli ATCC 53868): Good to Excellent

PR1MA  Potassium Phosphate, Dibasic, Anhydrous

CAS Number: 7758-11-4
Molecular Weight: 174.2
Chemical Formula: K2HPO4
Solubility: Water
Storage Temperature: Room Temperature


Research or further manufacturing use only, not for food or drug use.


  • Appearance: White Powder
  • Assay:  98.0 %
  • Chloride:  0.003 %
  • Heavy Metals :  5 ppm
  • Insoluble Matter:  0.01 %
  • Iron (Fe):  0.001 %
  • Loss on Drying @150 oC:  1.0 %
  • Nitrogen Compound:  0.001 %
  • pH (5%): 8.5 - 9.6
  • Sodium (Na):  0.05 %
  • Sulfate:  0.005 %

PR1MA  Tetracycline Hydrochloride

CAS Number: 64-75-5
Molecular Weight: 480.9
Chemical Formula: C22H24N2O8  HCl
Solubility: Water
Storage Temperature: Room Temperature


Bacteriostatic antibiotic with activity against gram positive and gram negative bacteria. Slightly soluble in water.

 
WARNING! This product can expose you to Tetracycline HCl, a chemical which is known to the State of California to cause developmental harm. For more information go to www.P65Warnings.ca.gov.

 
Research or further manufacturing use only, not for food or drug use.

  • Appearance: Yellow, Crystalline Powder
  • Identification (A, B, C): Pass Test
  • Specific Rotation: -255 - - 240 °
  • Crystallinity: Meets with specification
  • pH (1% Solution): 1.8 - 2.8
  • Loss on Drying (105 oC):  2.0 %
  • Epitetracycline:  3.0 %
  • 4-Ephianhydrotetracyline:  2.0 %
  • Anhydrotetracycline:  0.5 %
  • 2-Acetyl-2-Decarbamoyltetracycline:  1.5 %
  • Chlortetracycline:  0.5 %
  • Sum of impurities other than epitetracycline:  5.0 %
  • Assay of Tetracycline Hydrochloride (On Dried Basis):  900 µg/mg
  • Residual Solvent (1-Butanol):  5000 ppm
  • Residual Solvent (Acetone):  500 ppm
  • Grade: USP
  • Country of Origin: Report

PR1MA™ Nematode Growth Medium

 

Common Name: Nematode Growth Medium

Solubility: Water with Heat

Storage Temperature: Room Temperature

 

A standard formulation for use in Nematode growth studies.


Contains:

Agar 17.5 g/l
Sodium Chloride 3.0 g/l
Peptone 2.5 g/l
Cholesterol 0.005 g/l
Shipping Dimensions: 3.50 x 3.75 x 3.50
Shipping Weight: 1.2 lbs
 

Research or further manufacturing use only, not for food or drug use.

 

Specifications

  • Appearance: Light tan to pale yellow powder
  • Solubility: Clear Solution
  • pH (2.3% Solution @25 oC): Report
  • Agar: 17.5 g/l
  • Sodium Chloride: 3.0 g/l
  • Peptone: 2.5 g/l
  • Cholesterol: 0.005 g/l

 

Item#:
ASPRNGM

PR1MA  Trimethoprim

CAS Number: 738-70-5
Molecular Weight: 290.3
Chemical Formula: C14H18N4O3
Solubility: DMSO 50mg/ml, Methanol 12:1 mg/ml, Water 0.4 mg/ml
Storage Temperature: 2-8°C


Inhibits dihydrofolate reductase. Active against most gram negative and gram positive aerobic bacteria.


Research or further manufacturing use only, not for food or drug use.


  • Appearance: Off White to Light Yellow Crystalline Powder
  • Assay (Anhydrous): 98.5 - 101 %
  • Heavy Metals :  20 ppm
  • Loss on Drying:  1.0 %
  • Melting Point: 199 - 203 oC
  • Related Substances:  0.2 %
  • Sulfated Ash:  0.1 %

PR1MA SOB Broth

Solubility: Water
Storage Temperature: Room Temperature


SOB Broth is recommended for use in cultivation of recombinant strains of E. Coli. It is a nutritionally rich growth medium for use in the preparation and transformation of competent cells.


Add 28 grams of Broth in 1 liter of distilled water. Heat gently to dissolve the medium completely. Dispense in tubes and sterilize by autoclaving for 15 minutes.


Ingredients:

  • Casein Enzymic Hydrolysate: 20 g/L
  • Magnesium Sulfate: 2.4 g/L
  • Yeast Extract: 5 g/L
  • Potassium Chloride: 0.186 g/L
  • Sodium Chloride: 0.5 g/L
  • Final pH(25°:C): 7.0+0.2


Research or further manufacturing use only, not for food or drug use.


  • DCM Appearance: Homogeneous Free Flowing,Light Beige
  • PPM Appearance: Clear, no to light ppt, light yellow
  • pH (2.8 % Solution @25 oC): 6.8 - 7.2
  • Microorganism (E. coli ATCC 23724): Good to Excellent
  • Microorganism (E. coli ATCC 33694): Good to Excellent
  • Microorganism (E. coli ATCC 33849): Good to Excellent
  • Microorganism (E. coli ATCC 39403): Good to Excellent
  • Microorganism (E. coli ATCC 47014): Good to Excellent
  • Microorganism (E. coli ATCC 53868): Good to Excellent

PR1MA Tween 20

Common Name: Polyoxyethylenesorbitan Monolaurate
CAS Number: 9005-64-5
Solubility: Water
Storage Temperature: Room Temperature

Fatty acid composition:

  • Lauric acid: Approximately 50%
  • Balance: Myristic, Palmitic and Stearic acids.


Shipping Weight: 1.2 lbs
Shipping Dimensions: 2.00 x 6.00 x 2.00


Specifications

Appearance: Yellow to amber viscous liquid
1,4-Dioxane: ≤ 10 ppm
Acid Value: ≤ 2.20 mg KOH/g
Color, Gardner: ≤ 5.0
Hydroxyl Value: 96 - 108 mgKOH/g
Saponification Value: 40.0 - 50.0 mg KOH/g
Water: ≤ 3.00 %

Research or further manufacturing use only, not for food or drug use.

PR1MA Sulfamethoxazole

CAS Number: 723-46-6
Molecular Weight: 253.3
Chemical Formula: C10H11N3O3S
Solubility: Acetone
Storage Temperature: Room Temperature


Bacteriostatic antibiotic that inhibits nucleic acid synthesis in sensitive micro-organisms.


Research or further manufacturing use only, not for food or drug use.

  • Appearance: White to Off White Crystalline Powder
  • Infrared Spectrum: Conforms to Structure
  • Loss on Drying:  0.5 %
  • Residue on Ignition:  0.1 %
  • Related Substance (Sulfanilic acid):  0.10 %
  • Related Substance (Sulfanilamide):  0.10 %
  • Related Substance (Impurity C, A):  0.10 %
  • Related Substance (Impurity B):  0.10 %
  • Related Substance (Impurity F):  0.10 %
  • Any other Impurity:  0.10 %
  • Total impurities:  0.30 %
  • Assay (Titration): 99.0 - 101.0 %
  • Grade: USP
  • Country of Origin: Report

 

PR1MA Validamycin A 

CAS Number: 37248-47-8
Molecular Weight: 497.5
Chemical Formula: C20H35NO13
Solubility: DMSO, slightly soluble in ethanol
Storage Temperature: 2-8°C


Inhibits trehalase activity. Enhances trehalose accumulation in transgenic plants.


Research or further manufacturing use only, not for food or drug use.

  • Appearance: Light yellow loose powder
  • Assay:  64.0 %
  • pH: 2.5 - 5.0
  • Water:  5.0 %
  • Water Insoluble:  0.2 %

 

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