Molecular Biology Reagents/Chemicals

MIDSCI™ gives you ultra-pure, cost-competitive molecular biology reagents and lab chemicals for life science research; often with significant cost savings over Sigma Aldrich and other lab reagent providers.

PR1MA™ Chemicals can help with a wide variety of research-based requirements.
 
Use our glycerol for polyacrylamide gel electrophoresis. Eliminate Agrobacterium after inoculation with the carbenicillin we carry. Or detect β-Galactosidase activity with X-GAL which, when combined with the IPTG we stock, can be used to detect lac gene expression in cloning applications.
 
We also carry Luria Broth (Lennox), for the propagation and maintenance of E. coli; DTT, in order to maintain –SH groups in a reduced state; and high gel strength agar for tissue culture and microbiological work.

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PR1MA  Trimethoprim

CAS Number: 738-70-5
Molecular Weight: 290.3
Chemical Formula: C14H18N4O3
Solubility: DMSO 50mg/ml, Methanol 12:1 mg/ml, Water 0.4 mg/ml
Storage Temperature: 2-8°C


Inhibits dihydrofolate reductase. Active against most gram negative and gram positive aerobic bacteria.


Research or further manufacturing use only, not for food or drug use.


  • Appearance: Off White to Light Yellow Crystalline Powder
  • Assay (Anhydrous): 98.5 - 101 %
  • Heavy Metals :  20 ppm
  • Loss on Drying:  1.0 %
  • Melting Point: 199 - 203 oC
  • Related Substances:  0.2 %
  • Sulfated Ash:  0.1 %

PR1MA SOB Broth

Solubility: Water
Storage Temperature: Room Temperature


SOB Broth is recommended for use in cultivation of recombinant strains of E. Coli. It is a nutritionally rich growth medium for use in the preparation and transformation of competent cells.


Add 28 grams of Broth in 1 liter of distilled water. Heat gently to dissolve the medium completely. Dispense in tubes and sterilize by autoclaving for 15 minutes.


Ingredients:

  • Casein Enzymic Hydrolysate: 20 g/L
  • Magnesium Sulfate: 2.4 g/L
  • Yeast Extract: 5 g/L
  • Potassium Chloride: 0.186 g/L
  • Sodium Chloride: 0.5 g/L
  • Final pH(25°:C): 7.0+0.2


Research or further manufacturing use only, not for food or drug use.


  • DCM Appearance: Homogeneous Free Flowing,Light Beige
  • PPM Appearance: Clear, no to light ppt, light yellow
  • pH (2.8 % Solution @25 oC): 6.8 - 7.2
  • Microorganism (E. coli ATCC 23724): Good to Excellent
  • Microorganism (E. coli ATCC 33694): Good to Excellent
  • Microorganism (E. coli ATCC 33849): Good to Excellent
  • Microorganism (E. coli ATCC 39403): Good to Excellent
  • Microorganism (E. coli ATCC 47014): Good to Excellent
  • Microorganism (E. coli ATCC 53868): Good to Excellent

PR1MA Tween 20

Common Name: Polyoxyethylenesorbitan Monolaurate
CAS Number: 9005-64-5
Solubility: Water
Storage Temperature: Room Temperature

Fatty acid composition:

  • Lauric acid: Approximately 50%
  • Balance: Myristic, Palmitic and Stearic acids.


Shipping Weight: 1.2 lbs
Shipping Dimensions: 2.00 x 6.00 x 2.00


Specifications

Appearance: Yellow to amber viscous liquid
1,4-Dioxane: ≤ 10 ppm
Acid Value: ≤ 2.20 mg KOH/g
Color, Gardner: ≤ 5.0
Hydroxyl Value: 96 - 108 mgKOH/g
Saponification Value: 40.0 - 50.0 mg KOH/g
Water: ≤ 3.00 %

Research or further manufacturing use only, not for food or drug use.

Item#:
ASTWEEN20

PR1MA Sulfamethoxazole

CAS Number: 723-46-6
Molecular Weight: 253.3
Chemical Formula: C10H11N3O3S
Solubility: Acetone
Storage Temperature: Room Temperature


Bacteriostatic antibiotic that inhibits nucleic acid synthesis in sensitive micro-organisms.


Research or further manufacturing use only, not for food or drug use.

  • Appearance: White to Off White Crystalline Powder
  • Infrared Spectrum: Conforms to Structure
  • Loss on Drying:  0.5 %
  • Residue on Ignition:  0.1 %
  • Related Substance (Sulfanilic acid):  0.10 %
  • Related Substance (Sulfanilamide):  0.10 %
  • Related Substance (Impurity C, A):  0.10 %
  • Related Substance (Impurity B):  0.10 %
  • Related Substance (Impurity F):  0.10 %
  • Any other Impurity:  0.10 %
  • Total impurities:  0.30 %
  • Assay (Titration): 99.0 - 101.0 %
  • Grade: USP
  • Country of Origin: Report

 

PR1MA Validamycin A 

CAS Number: 37248-47-8
Molecular Weight: 497.5
Chemical Formula: C20H35NO13
Solubility: DMSO, slightly soluble in ethanol
Storage Temperature: 2-8°C


Inhibits trehalase activity. Enhances trehalose accumulation in transgenic plants.


Research or further manufacturing use only, not for food or drug use.

  • Appearance: Light yellow loose powder
  • Assay:  64.0 %
  • pH: 2.5 - 5.0
  • Water:  5.0 %
  • Water Insoluble:  0.2 %

 

PR1MA  Timentin [Ticarcillin Disodium Salt/Potassium Clavulanate Mixture 15:1 Ratio]

Common Name: Ticarcillin Disodium Salt/Potassium Clavulanate Mixture 15:1 Ratio
CAS Number: 4697-14-7; 61177-45-5
Molecular Weight: 428.39 / 665.7
Chemical Formula: C15H14N2Na2O 6S2 / C8H8KNO5
Solubility: Water
Storage Temperature: 2-8°C



Highly effective combination against gram negative bacteria such as Agrobacterium strains. -Lactamase inhibitor.


Plant cell culture tested. Hygroscopic.


Mixture Contains:

  • 1875 mg Ticarcillin to 125 mg potassium clavulanate (15:1 ratio)


Research or further manufacturing use only, not for food or drug use.
 
 

  • Appearance: White to Pale Yellow Crystalline Powder
  • Assay (Clavulanic Acid):  5.0 %
  • Assay (Ticarcillin):  75.0 %
  • Bacterial Endotoxins :  0.07 EU/mg
  • Identification: Conform
  • Particulate Matter: Conforms
  • pH: 5.5 - 7.5
  • Potency (Clavulanic Acid):  50 µg/mg
  • Potency Ticarcillin:  750 µg/mg
  • Proportion of Ticarcillin to Clavulanic acid: 14:1 - 16:1
  • Related Substances (Largest Impurity):  4.0 %
  • Related Substances (Second Major Impurity) :  2.5 %
  • Water (KF):  4.5 %

PR1MA™ Miller's LB Agar Capsules


Common Name:
Miller's LB Agar

Solubility:
Water with Heat

Storage Temperature:
Room Temperature


Super convenient. Eliminate messy weighing. Consistant and accurate measurement. Convenient premeasured and pre-mixed capsules. Prebuffered media eliminates pH adjustments.

Simply add 40 capsules to one liter of water, sterilize by autoclaving for 15 minutes at 121°C. Capsules contain all necessary ingredients for maintenance and propagation of E. Coli.

Ingredients:

Casein Digest Peptone: 10g/L
Yeast Extract: 5g/L
Sodium Chloride: 10g/L
Agar:15g/L
TRIS/TRIS HCl: 1.5g/L
Item#:
ASPR1MAMILLERLBAGAR
[5-Bromo-4-chloro-3-indolyl-β-D-galactoside]

PR1MA™ X-GAL [5-Bromo-4-chloro-3-indolyl-β-D-galactoside]

Common Name: 5-Bromo-4-chloro-3-indolyl-β-D-galactoside
CAS Number: 7240-90-6
Molecular Weight: 408.6
Chemical Formula: C14H15BrCINO6
Solubility: DMSO and DMF
Storage Temperature: -20°C


Chromogenic substrate of β-Galactosidase used in combination with IPTG for detection of β-Galactosidase activity in bacterial colonies. X-GAL is cleaved at the B1-4 bond between galactose and the 5-Bromo-4-chloro-3-indolyl portion of X-GAL by β-Galactosidase via hydrolysis. The cleavage of X-GAL results in the production of a water insoluble blue dichloro-dibromo-indigo precipitate. In cloning strategies with vectors such as Lambda-11, M13mp18 and 19, pUC18 and 19, pUR222 the E. coli lacZ gene is transformed to lac+ cells. After transformation the cells show β-Galactosidase activity in the presence of media containing X-GAL and IPTG. The insertion of a DNA fragment into the cloning sites of the lacZ gene results in the disruption of β-Galactosidase activity leading to the appearance of white colonies. Non-recombinant cells produce a blue indigo dye.


Water Content: 0.05% (K. Fisher)
Protect from light and moisture


Research or further manufacturing use only, not for food or drug use.


  • Appearance: White Powder
  • Identity: Conforms to structure
  • Purity (HPLC):  98.0 %
  • Solubility (2%, DMF): Clear and Colorless Solution
  • Specific Rotation: -63.0 - -60.0 °
  • Water Content (K.F):  0.5 %

PR1MA  Mitomycin C

CAS Number: 50-07-7
Molecular Weight: 334.3
Chemical Formula: C15H18N4O5
Solubility: Ethanol
Storage Temperature: 2-8°C
 

Antineoplastic antibiotic that acts as an alkylating agent after activation and also suppresses the synthesis of nucleic acids. A cell-cycle non specific agent, most active in the late G1 and early S phases.


WARNING! This product can expose you to Mitomycin-C, a chemical which is known to the State of California to cause cancer. For more information go to www.P65Warnings.ca.gov.


Research or further manufacturing use only, not for food or drug use.
 
 

  • Appearance: Blue violet solid
  • Identification (UV): Conforms
  • Purity-HPLC (RP CH3CN:H2O-0-100%):  99.0 %
  • Purity-HPLC (RP MEOH:H2O-90-10):  98.0 %
  • Purity-TLC (CH2CL2:MEOH- 9:1):  98.0 %
  • Solubility (10 mg/ml MEOH): Clear Blue Solution

PR1MA  MOPS [3-(N-morpholino)-propanesulfonic acid]

Common Name: 3-(N-Morpholino)propanesulfonic acid
CAS Number: 1132-61-2
Molecular Weight: 209.3
Chemical Formula: C7H15NO4S
Solubility: Water
Storage Temperature: Room Temperature


Molecular Biology Grade

  • Heavy Metals: <5 ppm
  • pKa: (20°C) 7.20 ± 0.2
  • pH Range: 6.5-7.9

Research or further manufacturing use only, not for food or drug use.
 

  • Appearance: White crystalline powder
  • Absorbance @250nm, 1M solution in H2O:  0.1
  • Absorbance 260nm:  0.2 nm
  • Absorbance 280nm:  0.2
  • Assay (Titration):  99.0 %
  • Endotoxin :  0.5 EU/mg
  • Heavy Metals :  5 ppm
  • Identification: Matches the reference
  • Iron (Fe):  5 ppm
  • Loss on Drying (105 oC):  1.0 %
  • Melting Point : 276.0 - 281.0 oC
  • pH: 2.5 - 5.0
  • pKa @ 20°C: 7.0 - 7.4
  • Residue on Ignition:  0.2 %
  • Solubility: Clear, colorless solution

PR1MA Acrylamide, 40% Solution

CAS Number: 79-06-1; 7732-18-5
Storage Temperature: 2-8°C


A 40% (w/v) Acrylamide solution.


WARNING! This product can expose you to Acrylamide, a chemical which is known to the State of California to cause cancer, developmental and reproductive harm. For more information go to www.P65Warnings.ca.gov.


Research or further manufacturing use only, not for food or drug use.


  • Appearance: Clear, Colorless Solution
  • Acrylic Acid:  0.003 %
  • Conductivity:  5 µmhos
  • DNase, RNase, protease: Not Detected
  • Electrophoresis: Pass Test
  • pH @25 °C: 6.0 - 7.0

Click here for Agarose with a better price and ready to ship today!



Bulls Eye Agarose GP2 is highly refined and highly purified. Each lot is physically tested and verified for performance in its designated application. 

Reasons why Bulls Eye Agarose GP2 will work for you:


1) Obviously Bulls Eye Agarose GP2 gives you everything you want in a High Quality General Purpose Agarose at a fair price. 
2) Bulls Eye Agarose GP2 is produced to deliver lot-to-lot consistency
3) Quality and Purity
4) Packaged in 500g Bottles Bulls Eye Agarose saves the lab money without sacrificing quality.
Bulls Eye Agarose GP2 offers you:

  • General purpose electrophoresis and blotting
  • Excellent separation, resolution, and clarity
  • Low absorption and background from staining reagents
  • High gel strength for safe and easy handling
  • Highly refined and purified
  • Greater thermal stability
  • Packaged with spec sheet, prep instructions, and MSDS

Physical Specifications:

CAS#

9012-36-6

Moisture

 7%

Ash

 4.5%

Sulfate

 0.1%

Clarity @ 1.5% (NTU)

4

Gel Strength @1.0% (g/cm2)

 1000

Gel Strength @1.5% (g/cm2)

 2200

Gelling Temperature @ 1.5%

36°C ± 1.5°C

Melting Temperature @ 1.5%

88°C ± 1.5°C

 

Base Pair Data

1X TAE Buffer

Gel Concentration %

1X TBE Buffer

50-1500bp

2.0

50-1000bp

100-2500bp

1.75

100-2000bp

200-5000bp

1.5

150-3000bp

250-10,000bp

1.25

200-4000bp

300-16,000bp

1.0

300-8000bp

500-20,000bp

0.75

500-12,000bp

Item#:
ASPCRAGAROSE1
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