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Actions- Reagents for denaturing and loading RNA samples onto a formaldehyde gel, using MOPS as a buffer
- RNA sample is dissolved in 10µl of DEPC water and mixed with 35µl of denaturing solution. Heat the sample to 65°C for 5 min. Once the solution has cooled, add 5µl of loading dye. The sample is now ready to load into the gel.
- DNase/RNase/Protease free
- Used for agarose electrophoresis of DNA, RNA or nucleic acids
- Contains 3 tracking dyes and 15% Ficoll in a special Tris dye
-
- Light blue - around 4000bp in 1% agarose
- Indigo - around 600bp in 1% agarose
- Magenta - around 150bp in 1% agarose
- DNase/RNase/Protease free
This brand is being discontinued, please contact us for other options.
Need great dNTPs at a great price? Try our PR1MA™ dNTPs
- Mix of dATP, dCTP, dGTP, dTTP
- Each nucleotide is at a concentration of 12.5 mM
- Ready-to-use molecular grade dNTP solution for use in DNA polymerization, DNA labelling and sequencing processes
- High purity: >98% by HPLC
- Supplied in solution at pH 7.5
- dNTPs are stable at -20°C, avoid multiple freeze/thawing (For long-term usage, aliquoting is recommended)
- Functionally tested with thermostable polymerases
This brand is being discontinued and will only be available while supplies last.
Need great dNTPs at a great price?
Try our PR1MA™ dNTPs
- Ready-to-use molecular grade dNTP solution for use in DNA polymerization, DNA labeling and sequencing processes
- High purity: >98% by HPLC
- Supplied in solution at pH 7.5
- dNTPs are stable at -20°C, avoid multiple freeze/thawing (For long-term usage, aliquoting is recommended)
- Functionally tested with thermostable polymerases
This brand is being discontinued and will only be available while supplies last.
Need great dNTPs at a great price?
Try our PR1MA™ dNTPs
- Mix of dATP, dCTP, dGTP, dTTP
- Each nucleotide is at a concentration of 100 mM
- Ready-to-use molecular grade dNTP solution for use in DNA polymerization, DNA labelling and sequencing processes
- High purity: >98% by HPLC
- Supplied in solution at pH 7.5
- dNTPs are stable at -20°C, avoid multiple freeze/thawing (For long-term usage, aliquoting is recommended)
- Functionally tested with thermostable polymerases
dNTP SET
(dATP, dCTP, dGTP, dTTP)
100 mM
Cat. No. |
Size |
dNTP sets each 100mM Solutions |
BE511109 |
20x 4x 250 µL |
dATP, dCTP, dGTP, dTTP |
BE511120 |
4x 2 mL |
dATP, dCTP, dGTP, dTTP |
Store at 20°C For in-vitro laboratory use only
Components Volume dATP (100mM) 250 µL dCTP (100mM) 250 µL dGTP (100mM) 250 µL dTTP (100mM) 250 µL General Description Ready-to-use molecular grade dNTP solution for use in DNA polymerization, DNA labelling and sequencing processes. Features High purity: >98% by HPLC. Supplied in solution at pH 7.5. Storage Conditions dNTPs are stable at 20oC in a constant temperature freezer. Avoid multiple freeze/thawing. For long-term usage, aliquoting is recommended. Quality control Functionally tested with thermostable polymerases. |
This brand is being discontinued, please contact us for other options.
Need great dNTPs at a great price? Try our PR1MA™ dNTPs
- Mix of dATP, dCTP, dGTP, dTTP
- Each nucleotide is at a concentration of 10 mM
- Ready-to-use molecular grade dNTP solution for use in DNA polymerization, DNA labelling and sequencing processes
- High purity: >98% by HPLC
- Supplied in solution at pH 7.5
- dNTPs are stable at -20°C, avoid multiple freeze/thawing (For long-term usage, aliquoting is recommended)
- Functionally tested with thermostable polymerases
This brand is being discontinued and will only be available while supplies last.
- Higher sensitivity
- Use in the same way as EtBr in agarose gel electrophoresis
- Detection of DNA and RNA
- Safest DNA stain by far
- Low cost
- 10,000X
- Excitation wavelengths at 290nm and 490nm
DNA SafeStain Plus is a highly sensitive green fluorescent DNA/RNA staining reagent for detecting nucleic acids in agarose and polyacrylamide gels. This unique stain gives high sensitivity for detection of double-stranded or single-stranded DNA and RNA. Gels can be post-stained or the stain can be added to gels during gel casting or to the gel running buffer. DNA SafeStain Plus has two excitation wavelength peaks at about 290nm and 490nm, and an emission wavelength at 530nm, making it compatible with a standard UV light box, a blue-light transilluminator, or a gel reader equipped with visible light excitation; such as, a 488 nm laser-based gel scanner.
DNA SafeStain Plus is in a 10,000X concentrated format that can be easily diluted 10,000 times for use in precast gel staining, or 5,000 times for use in post gel staining.
PR1MA™ Reverse Transcriptase, RNase H -
PR1MA™ reverse transcriptase (RT) is an RNA-dependent DNA polymerase ideal for use in RT-PCR and first-strand synthesis of complementary DNA (cDNA) for generation of cDNA libraries from single-stranded RNA, DNA, or RNA:DNA hybrids.
- Decreased RNase H activity enables longer cDNA synthesis (>5 kb).
- Lacks 3’ to 5’ exonuclease activity
- Optimal temperature at 42°C
- Temperature range: 40 to 50°C
- Heat inactivation: 70°C for 20 minutes
- Storage temperature: -20°C
- 10X reaction buffer included
Buffer composition
- 50% glycerol
- 50 mM Tris-HCl
- 50 mM KCl
- 1 mM DTT
- 0.1 mM EDTA
- pH = 7.5
*These products are intended for research use only, not for therapeutic or diagnostic purposes in humans or animals. The safety and efficacy of these products in diagnostic or other clinical uses has not been established.
IBI DEPC-Treated, Nuclease-Free Water is ideally suited for all applications in molecular biology lab including PCR, RT-PCR, restriction enzyme
assays, modifying enzyme assays, transfection, cloning, transformation, and specifically for all RNA assays.
IBI DEPC-Treated Water is manufactured under stringent conditions. The purification process for this product includes continuous
deionization, reverse osmosis, UV-treatment, 0.1µm filtration, followed by steam sterilization in an autoclave. The product is re-autoclaved
to ensure the breakdown of DEPC and release of carbon dioxide that may cause the pH to shift.
IB42200 |
DEPC Treated Water, nuclease free, 125mL |
IB42201 |
DEPC Treated Water, nuclease free, 24 x 125mL |
IB42202 |
DEPC Treated Water, nuclease free, 48 x 125 mL |
IB42210 |
DEPC Treated Water, nuclease free, 500 mL |
IB42211 |
DEPC Treated Water, nuclease free, 10 x 500 mL |
IB42212 |
DEPC Treated Water, nuclease free, 20 x 500 mL |
IB42220 |
DEPC Treated Water, nuclease free, 1L |
IB42221 |
DEPC Treated Water, nuclease free, 6 x 1L |
IB42222 |
DEPC Treated Water, nuclease free, 12 x 1L |
IB42230 |
DEPC Treated Water, nuclease free, 2L |
IB42231 |
DEPC Treated Water, nuclease free, 6 x 2L |
IB42240 |
DEPC Treated Water, nuclease free, 10L |
- Tracks the migration progression of your sample during polyacrylamide electrophoresis
- Loading dye migrates independently of the samples, making it easier to estimate the migration of proteins
- DNase/RNase/Protease free
- Safely excise bands from gels
- Avoid cross contamination
- One-handed operation for quick and accurate gel cutting
- Safer than using razor blades and won't scratch transilluminators
Cut gel with pipet tip on the end of a standard 1000µl pipettor. Gel piece is suspended in tip when pipettor is lifted from the gel. Expel the gel piece by pushing the pushbutton on the pipettor. Tip is ejected in normally using the ejector button.
- Safely excise bands from gels
- Avoid cross contamination
- One-handed operation for quick and accurate gel cutting
- Safer than using razor blades and won't scratch transilluminators
Cut gel with pipet tip on the end of a standard 1000µl pipettor. Gel piece is suspended in tip when pipettor is lifted from the gel. Expel the gel piece by pushing the pushbutton on the pipettor. Tip is ejected in normally using the ejector button.