PR1MA™ SmartGlow™ DNA Safe Stain

• Replaces hazardous Ethidium Bromide (EtBr)
• Better sensitivity than EtBr
• Detect as little as 0.1ng of DNA
• Two types available
• PS Pre Stain
• LD Loading Dye
• Excitation by UV light or blue light
• Compatible with Accuris SmartBlue™ Transilluminator
• Ships at ambient temperature (stored at 4°C)

PR1MA™ SmartStain™ PS (Pre-Stain) can be used as a direct replacement for Ethidium Bromide in agarose and polyacrylamide gel electrophoresis. The stain emits green fluorescence when bound to dsDNA or ssDNA and emits red fluorescence when bound to RNA. PR1MA™ SmartStain™ PS exhibits excitation peaks at 290 nm and 490 nm, allowing it to be used with UV and blue light.

Protocol:

• Prepare 100 mL of agarose or polyacrylamide solution.
• Add 5 uL of PR1MA™ SmartStain™ to the gel solution before pouring gels.
• For enhanced results, add PR1MA™ SmartStain™ PS to the running buffer at a ratio of 5 uL per 100 mL. Adding PR1MA™ SmartStain™ PS to the running buffer will result in increased sensitivity and better detection of small quantities of nucleic acid.
• After electrophoresis is complete, view the gel using a UV or blue light illuminator

• Convenient cDNA synthesis and PCR in a single tube from 1 pg total RNA
• Formulated for highly specific and sensitive RT-PCR from any RNA templates
• Incorporates thermostable reverse transcriptase and Hot-Start Taq for preparation at room temperature

The PR1MA™ One-Step RT-PCR Kit has been formulated for cDNA synthesis and subsequent PCR in a single tube for end-point analysis. This latest generation RT-PCR Kit consists of an MMLV-derived, thermostable Reverse Transcriptase (45°C to 55°C), an advanced RNase Inhibitor and PR1MA™ Hot Start Taq for ultra-sensitive one-step RT-PCR from as little as 1pg total RNA starting material.

The optimized buffer chemistry allows for efficient reverse transcription and PCR of problematic sequences with significant secondary structure (such as GC-rich targets). The PR1MA One-Step RT-PCR Kit is ideal for determining the presence or absence of RNA templates and quantifying expression through qualitative analysis of RNA transcription levels. The kit also efficiently synthesizes double-stranded cDNA for subsequent gene expression analysis.

Proudly made in the USA! Click here for more made in America products!
 

 Item PR2110-100 has been discontinued by the manufacturer;
however, we will be continuing to supply the other quantities.

 

In place of item PR2110-100, please order items PR2110-50 or PR2110-200

PR1MA™ qMAX™ cDNA Synthesis Kits

PR1MA™ now offers two cDNA Synthesis Kits, to meet a range of requirements.
 
The original cDNA Synthesis Kit is a 2-tube format for easy reaction setup and is ideal for 4 pg to 0.5 µg of input RNA. One tube includes our exceptionally stable Reverse Transcriptase combined with a potent RNAse inhibitor, and the other tube contains a 5X reaction buffer with an optimal mixture of anchored oligo (dT) primers and random hexamers to produce a non-biased population of cDNA. 
 
The 1st Strand cDNA Synthesis Flex Kit includes four components: a high-capacity Reverse Transcriptase, an optimized 5X buffer, separate solutions of oligo (dT) primers, and random hexamer primers. This multi-component format is ideal for 10 pg to 2.0 µg of input RNA and allows for greater flexibility in assay design.

 
PR1MA™ qMax cDNA Synthesis Kits
  

Item	Description	Volume (20 µL)
PR2100-C-S	PR1MA™ qMax™ cDNA Synthesis Kit	10 Reactions (Sample)
PR2100-C-25	PR1MA™ qMax™ cDNA Synthesis Kit	25 Reactions
PR2100-C-100	PR1MA™ qMax™ cDNA Synthesis Kit	100 Reactions
PR2100-C-250	PR1MA™ qMax™ cDNA Synthesis Kit	250 Reactions

 

PR1MA™ qMax First Strand cDNA Synthesis Flex Kits  
 

Item	Description	Volume (20 µL)
PR2110-S	PR1MA™ qMax™ First Strand cDNA Synthesis Flex Kit	10 Reactions (Sample)
PR2110-50	PR1MA™ qMax™ First Strand cDNA Synthesis Flex Kit	50 Reactions
PR2110-100	PR1MA™ qMax™ First Strand cDNA Synthesis Flex Kit	100 Reactions
PR2110-200	PR1MA™ qMax™ First Strand cDNA Synthesis Flex Kit	200 Reactions

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PR1MA™ dNTPs

• Supplied as a ready-to-use 40 mM mix or a set of 4 separate 100 mM solutions
• Free of impurities and inhibitors that reduce sensitivity and yield
• No nuclease, protease or nickase activity
• >99% pure, purified by HPLC
• 24-month shelf life

PR1MA™ dNTP's are purified by HPLC in a strict process that results in greater than 99% purity. The stringent purification process eliminates PCR inhibitors such as tetraphosphates and pyrophosphates that can interfere with the sensitivity of your PCR and reduce yields.
The 40 mM dNTP Mix is a single tube that contains premixed dNTPs at a concentration of 10 mM each. The 100 mM dNTP Set contains four individual tubes, one each of dATP, dCTP, dGTP and dTTP. The nucleotides are supplied in ultra-pure water as an ammonium salt. Both the set and mix are stable for 24 months when stored at -20ºC.

Extensive quality control testing is performed to ensure the dNTPs are free of nuclease, protease and nickase activity. Each lot is performance tested in standard PCR, long PCR and qPCR reactions to assess reproducibility and sensitivity.

PR1MA™ dNTP Mix

PR1MA™ dNTP Set  

Item	Description	Volume
PR3101-S-1	PR1MA™ 100 mM dNTP Set, dATP, dCTP, dGTP, dTTP	250 uL / 25 umol each
PR3101-S-4	PR1MA™ 100 mM dNTP Set, dATP, dCTP, dGTP, dTTP	1 mL / 100 umol each
PR3101-S-20	PR1MA™ 100 mM dNTP Set, dATP, dCTP, dGTP, dTTP	20 x 250 uL / 25 umol each

 

Proudly made in the USA! Click here for more made in America products!
  

IBI RT-PCR Certified and Nuclease-Free Water is ideal for all applications in a molecular biology lab including PCR, RT-PCR, restriction enzyme
assays, modifying enzyme assays, transfection, cloning, transformation, and all general molecular biology lab procedures.

IBI PCR Grade water is manufactured under stringent conditions. The purification process includes continuous deionization, reverse osmosis,
UV-treatment, 0.2µm filtration, followed by steam sterilization in an autoclave.

PR1MA™ CRISPR/Cas9 Nucleases

The RNA-guided endonuclease Cas9, associated with Type II CRISPR/Cas systems, site-specifically digests DNA using a single guide RNA (sgRNA) which it binds to direct it to the complementary sequence. MIDSCI™ offers two traditional versions of Streptococcus pyogenes Cas9 nuclease: CRISPR/Cas9, ideal for in vitro DNA digestion, and CRISPR/Cas9 NLS, for in vivo nuclear localization.

• Optimal temperature: 37°C
• Heat inactivation: 65°C for 20 minutes
• Storage temperature: -20°C

Buffer composition

• 50% glycerol
• 50 mM Tris-HCl
• 50 mM KCl
• 1 mM DTT
• 0.1  mM EDTA
• pH = 7.5 

*These products are intended for research use only, not for therapeutic or diagnostic purposes in humans or animals. The safety and efficacy of these products in diagnostic or other clinical uses has not been established.

Proudly made in the USA! Click here for more made in America products!
  

PR1MA™ Reverse Transcriptase

PR1MA™ reverse transcriptase (RT) is an RNA-dependent DNA polymerase that can be used for complementary DNA (cDNA) synthesis from an RNA template and is ideal for use in molecular amplification assays. PR1MA™ RT is a robust enzyme that works in a broad range of temperatures (40 - 72°C) and has RNase H activity.

• Optimal temperature: 55°C
• Heat inactivation: 75°C for 20 minutes
• Glycerol-free buffer available
• Storage temperature: -20°C (standard buffer)
• 10X Isothermal buffer included

Standard Buffer Composition

• 50% glycerol
• 10 mM Tris-HCl
• 100 mM KCl
• 1 mM DTT
• 0.1  mM EDTA
• pH = 7.5

Important note: Please be sure to use the buffer provided with this product to ensure optimal results.

*These products are intended for research use only, not for therapeutic or diagnostic purposes in humans or animals. The safety and efficacy of these products in diagnostic or other clinical uses has not been established.   
 

Proudly made in the USA! Click here for more made in America products!
  

PR1MA™ Reverse Transcriptase, RNase H -

PR1MA™ reverse transcriptase (RT) is an RNA-dependent DNA polymerase ideal for use in RT-PCR and first-strand synthesis of complementary DNA (cDNA) for generation of cDNA libraries from single-stranded RNA, DNA, or RNA:DNA hybrids.

• Decreased RNase H activity enables longer cDNA synthesis (>5 kb).
• Lacks 3’ to 5’ exonuclease activity
• Optimal temperature at 42°C
• Temperature range: 40 to 50°C
• Heat inactivation: 70°C for 20 minutes
• Storage temperature: -20°C
• 10X reaction buffer included 

Buffer composition

• 50% glycerol
• 50 mM Tris-HCl
• 50 mM KCl
• 1 mM DTT
• 0.1  mM EDTA
• pH = 7.5

*These products are intended for research use only, not for therapeutic or diagnostic purposes in humans or animals. The safety and efficacy of these products in diagnostic or other clinical uses has not been established.

Proudly made in the USA! Click here for more made in America products!
  

This brand is being discontinued and will only be available while supplies last.

Need a great DNA Safe Stain at a great price?
Try PR1MA! Click here to order.

Bullseye DNA SafeStain,10,000x 

• Safest DNA stain by far
• Replaces EthBr in agarose gel electrophoresis
• Detection of DNA and RNA
• Excitation at 290 nm & 490 nm
• Emission at 530 nm

DNA SafeStain is a new and safe nucleic acid stain for visualization of double-stranded DNA, single-stranded DNA, and RNA in agarose gels. The dyes are developed to replace toxic ethidium bromide (EthBr, a potent mutagen), commonly used in gel electrophoresis for visualization of nucleic acids in agarose gels. DNA SafeStain is non-carcinogenic by the Ames-test. The results are negative in both the mouse marrow chromophilous erthrocyte micronucleus and mouse spermary spermatocyte chromosomal aberration tests.

DNA SafeStain emits green fluorescence when bound to DNA. It has two excitation wavelength peaks when bound to nucleic acid, at 290nm and 490nm and the emission can be detected at 530nm.

Storage:
Store under dark at 4°C or room temperature.

User Instruction: 
DNA SafeStain can be used in the exact same way as EtBr in agarose gel electrophoresis. For example, add 100µl to 1 liter of 1X running buffer (1xTAE or 1x TBE), and use this DNA SafeStain containing buffer to make the agarose gel and run the gel.

Special Notes: 
1. For best results, dilute the DNA SafeStain 1:10,000 in your gel running buffer and use this dye-containing running buffer to make your agarose gel and to run the gel.

2. As is the case with EtBr; add more DNA SafeStain, if brighter DNA bands are desired. To view the results, any conventional DNA gel viewing light box or gel documentation system should work well with the DNA SafeStain; although, using a LED light instead of a UV light source is preferred due to the harmful nature of UV light.

This Product is For Research Use Only

*For the proper disposal of this product, follow University or Company Guidelines.

Bullseye RT 2X Master Mix

• Up to 9kb cDNA synthesis
• Ensures sample to sample consistency
• Large RNA sample volume capacity
• Ready to use

Size: 100 rxns

RT 2X Master Mix is a proprietary, ready-to-use master mix for first-strand cDNA synthesis in a 2X concentration. This optimized reaction mix contains ribonuclease inhibitor, dNTPs, and a balanced concentration for oligo(dT) and random primers. The ribonuclease inhibitor effectively protects RNA template from degradation. The oligo(dT) anneals selectively to the poly(A) tail of mRNAs and the random primers do not require the presence of poly(A) and they are utilized for the transcription of mRNA 5-end regions. The resultant cDNA can be directly used as template in different PCR experiments.

Kit Components

Storage

• Store at -20°C in a frost-free freezer.

Application

• cDNA synthesis
• Construction of cDNA libraries
• Generation of probes for hybridization 

Protocol

1. Thaw RNA templates and all reagents on ice. Mix each solution by vortexing.
2. Assemble the following components in a tube on ice, and mix well:

1. Heat the mixture at 65°C for 5 mins and incubate on ice for at least 1 min.
2. Collect all components by a brief centrifugation and add 1 µl of the EasyScript RTase to the tube.
3. Mix well and collect all the components by a brief centrifugation.
4. Incubate the tube at room temperature for 10 min for annealing.
5. Perform cDNA synthesis by incubating the tube for 50 min at 42°C.
6. Stop the reaction by heating it at 85°C for 5 min.
7. Chill on ice. The newly synthesized first-strand cDNA is ready for immediate downstream applications. 

Bullseye EasyScript cDNA Synthesis Kit

Application

• First strand cDNA synthesis for PCR
• Construction of cDNA libraries
• Generation of probes for hybridization

EasyScript cDNA Synthesis Kit is a complete system for the efficient synthesis of first strand cDNA from RNA templates. The recombinant RNasin Ribonuclease Inhibitor, supplied with the kit effectively protects RNA template from degradation.

The kit is also supplied with both oligo(dT) and random primers. The oligo(dT) anneals selectively on the poly(A) tail of mRNA. Random primers do not require the presence of poly(A). Therefore, they can be used for transcription of the 5'-end regions of mRNA. Gene-specific primers may also be used with the kit. The first strand of cDNA can be directly used as a template in PCR.

EasyScript Reverse Transcriptase (RTase) within the kit is a genetically modified form of Moloney Murine Leukemia Virus Reverse Transcriptase (M-MLV). Both the EasyScript and EasyScript Plus are RNase H deficient (negative). The enzyme is purified from bacteria host as a single holoenzyme of 71 kDa with the capacity of first-strand cDNA synthesis of template up to 9kb and can be used for routine cDNA synthesis.

Kit Components

Storage Buffer
 
50 mM Tris-HCl (pH 8.3), 100 mM NaCl, 0.1 mM EDTA, 5 mM DTT, 0.1% (v/v) Triton X-100, and 50% (v/v) glycerol.

Storage
 
Store at -20°C in a frost-free freezer. Multiple freezing and thawing of RNA should be avoided. Keep RNA on ice all the time. It is recommended that the first strand cDNA synthesis is carried out under conditions where RNase contamination has been eliminated.

General Protocol

RT-PCR reactions should be assembled in a RNA-free environment. The use of "clean", automatic pipettes designated for PCR and aerosol resistant barrier tips are recommended. 
1. Thaw template RNA and all reagents on ice. Mix each solution by vortexing, and centrifuge briefly to collect residual liquid from the sides of the tubes.
2. Prepare the following reaction mixture in a PCR tube on ice:

3. Incubate at 25°C for 10 minutes if random primer is used. Omit this step if Oligo(dT) primer or sequence specific primer are used.
4. Incubate the mixture at 42°C for 60 minutes.
5. Stop the reaction by heating at 85°C for 5 minutes.
6. Chill on ice. The newly synthesized first-strand cDNA now can be used directly for PCR amplification.

Notes
 
1. Isolation of poly(A)+ RNA from total RNA is not mandatory; however, doing so may improve the yield and purity of the final product.
2. RNA sample must be free of contaminating genomic DNA.
3. Unlike the oligo(dT) priming, which usually requires no optimization, the ratio of a random primer to RNA is critical in terms of the average length of cDNA synthesized in the reaction. Increasing the ratio of random primer/RNA will result in higher yield of shorter (~500 bp) cDNA, whereas decreasing this ratio will produce longer products.

4. The synthesized cDNA should be stored at -20°C.

Bullseye EasyScript™ Reverse Transcriptase

Application

• Synthesis cDNA froma single-stranded RNA or DNA primer extension
• Sequencing dsDNA
• cDNA library
• Template production for use in PCR
• 3'-end labeling of duplex DNA via end-filling reactions

EasyScript™ Reverse Transcriptase is a genetically modified form of Moloney Murine Leukemia Virus Reverse Transcriptase (M-MLV). Both the EasyScript™ and EasyScript™ Plus are RNase H deficient (negative). The enzyme is purified from bacteria h o s t as a single holoenzyme of 71 kDa with the capacity of first-strand cDNA synthesis of template up to 9kb and can be used for routine cDNA synthesis.

Kit Components

Storage Buffer
 
50 mM Tris-HCl (pH 8.3), 100 mM NaCl, 0.1 mM EDTA, 5 mM DTT, 0.1% (v/v) Triton X-100, and 50% (v/v) glycerol.

Storage
 
Store at -20°C in a frost-free freezer. Multiple freezing and thawing of RNA should be avoided. Keep RNA on ice all the time. It is recommended that the first strand cDNA synthesis is carried out under conditions where RNase contamination has been eliminated.

General Protocol

RT-PCR reactions should be assembled in a RNA-free environment. The use of "clean", automatic pipettes designated for PCR and aerosol resistant barrier tips are recommended.

1. Thaw template RNA and all reagents on ice. Mix each solution by vortexing, and centrifuge briefly to collect residual liquid from the sides of the tubes. 
2. Prepare the following reaction mixture in a PCR tube on ice:

3. Incubate at 25°C for 10 minutes if random primer is used. Omit this step if Oligo(dT) primer or sequence specific primer are used.
4. Incubate the mixture at 42°C for 60 minutes.
5. Stop the reaction by heating at 85°C for 5 minutes. 
6. Chill on ice. The newly synthesized first-strand cDNA now can be used directly for PCR amplification.

Notes:
1. Isolation of poly(A)+ RNA from total RNA is not mandatory; however, doing so may improve the yield and purity of the final product. 
2. RNA sample must be free of contaminating genomic DNA.
3. Unlike the oligo(dT) priming, which usually requires no optimization, the ratio of a randomprimer to RNA is critical in terms of the average length of cDNA synthesized in the reaction. Increasing the ratio of random primer/RNA will result in higher yield of shorter (~500 bp) cDNA, whereas decreasing this ratio will produce longer products.

4. The synthesized cDNA should be stored at -20°C.