PR1MA™ SmartCheck DNA Ladders

• Ready-to-use formulation includes loading buffer and tracking dye
• 500 µL suitable for 100 lanes (5 µL per lane)
• Higher intensity reference bands
• Ultra pure production allows economical ambient shipping

Technical Details
 

Protocol: Briefly vortex the tube and use 5 µL per lane. Additional loading buffer is not required.

Concentration: 0.1 mg/mL

Tracking dye: bromophenol blue and xylene cyanol

Buffer formulation: 10mM Tris-HCl (pH 8.0), 5mM EDTA, 12.5% glycerol, 0.008% bromophenol blue, 0.008% xylene cyanol

Shipping and Storage: SmartCheck™ DNA Ladders are shipped at ambient temperature. On arrival, store at -20°C for optimum stability and long-term storage up to 15 months.

Production: SmartCheck™ ladders are produced from proprietary plasmids, digested to completion. A multistep chromatography method is used to ensure purity and DNA quality.

Quality Control: Each lot of SmartCheck™ DNA Ladders is tested with a spectrophotometer to confirm DNA concentration, as well as agarose electrophoresis to confirm band sharpness.

Item	Description	Volume
PR4005-100	PR1MA™ SmartCheck™ 50bp DNA Ladder	500 µL / 100 Lanes
PR4005-500	PR1MA™ SmartCheck™ 50bp DNA Ladder	5 x 500 µL / 500 Lanes
PR4010-100	PR1MA™ SmartCheck™ 100bp DNA Ladder	500 µL / 100 Lanes
PR4010-500	PR1MA™ SmartCheck™ 100bp DNA Ladder	5 x 500µL / 500 Lanes
PR4100-100	PR1MA™ SmartCheck™ 1kb DNA Ladder	500 µL / 100 Lanes
PR4100-500	PR1MA™ SmartCheck™ 1kb DNA Ladder	5 x 500 µL / 500 Lanes

  

Bullseye 1Kb DNA Ladder, Logic

• Ready to use
• Contains 16 DNA bands: 100bp-10Kb
• 920ng DNA/6µl/loading
• Easy quantification of DNA fragments
• Stable at room temperature
• Supplied with 6x sample loading buffer

Bullseye 1Kb DNA Ladder, Logic contains 16 DNA bands ranging from 100bp to 10Kb, formulated so that each band contains an amount of DNA that correlates logically to its size, allowing the user to estimate both the size and the quantity of specific fragments at a glance. It is particularly useful for protocols such as probe labeling, DNA sequencing and optimizing insert/vector ratio in ligation reactions, where DNA concentration must be taken into consideration.

Size: 1200µl
Storage: Store at -20°C.
Concentration: 920ng/6µl

Loading Buffer Composition:

10mM Tris-HCl
1mM EDTA (pH 8.0)
0.02% Bromophenol blue
0.02% Xylene cyanol
5% Glycerol

Usage: Add 6µl of Bullseye 1Kb DNA Ladder, Logic directly to wells designated for markers, which will yield the amount as indicated in the picture. You may use more than 6µl of ladder, depending on well size and level of dye used to visualize the bands. Calculate the amount for each band according to your loading volume.

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• A unique combination of PCR products and a number of proprietary plasmids digested with appropriate restriction enzymes to yield 11 fragments, suitable for use as molecular weight standards for agarose gel electrophoresis.
• The DNA includes fragments ranging from 100-1,500 base pairs. The 500 and 1,500 base pair bands have increased intensity to serve as reference points.
• The approximate mass of DNA in each band is provided (0.5µg a load) for approximating the mass of DNA in comparably intense samples of similar size.

Source:

• PCR products and double-stranded DNA digested with appropriate restriction enzymes, are phenol and equilibrated to 10nM Tris-HCl (pH 8.0) and 10mM EDTA.

Range: 100-1,500 bp (DL1); 250-10,000 bp (DL5)
Number of bands: 11 (DL1); 13 (DL5)
Concentration: 100 µg/mL
Package: 50 µg/500 µL
Recommended Load: 5 µL / well
Contains orange G & xylene cyanol FF as tracking dyes. (DL1)
Contains bromophenol blue & xylene cyanol FF as tracking dyes. (DL5)
Storage:

• Store at 25°C for 6 months
• Store at 4°C for 12 months

Store at -20°C for 24 months

Bullseye 100bp DNA Ladder

• Ready to use
• Contains 11 DNA bands: 100-1500bp.
• Clearly identifiable 500bp band as reference
• 500ng DNA/6µl/loading
• Easy to load
• Stable at room temperature
• Supplied with 6x sample loading buffer

Bullseye 100bp DNA Ladder consists of 11 DNA fragments ranging in size from 100-1500 base pairs (bp). 6µl will yield at least 30ng DNA in any single band. The intensity of the 500bp band has been increased to serve as a reference for easy identification.

Size: 1200µl
Storage: Store at -20°C.
Concentration: 500ng/6µl
Loading Buffer Composition:

10mM Tris-HCl
1mM EDTA (pH 8.0)
0.02% Bromophenol blue
0.02% Xylene cyanol
5% Glycerol

Usage: Add at least 6µl Bullseye 100bp DNA Ladder directly to wells designated for markers. You may need more than 6µl of ladder, depending on well size and level of intensity needed to visualize the bands.

Please give us a call for a sample.