PR1MA™ qMAX Green qPCR Mix with Blue Tracking Dye

• Superior sensitivity and fast cycling with exceptional results
• Ideal for low copy number templates
• Early Ct values and detection across a broad dynamic range
• Ready to use 2X Master Mix
• Includes PR1MA™ Hot Start Taq Polymerase for greater specificity and accuracy

Supplied as a ready-to-use 2X master mix, PR1MA™ qMax Green has been engineered for high sensitivity, fast cycling, and excellent reproducibility. PR1MA™ Hot Start Polymerase provides accurate PCR of a variety of templates including low copy number and difficult sequences, while the proprietary qMax Green intercalating dye exhibits higher fluorescence and lower PCR inhibition than other popular green dyes.

These two components are supported by a specially formulated buffer with an exacting combination of salts, PCR enhancers, stabilizers, and pH that results in earlier Ct values and a high specificity across a broad dynamic range.

*Please note these products ship on dry ice.  Appropriate shipping charges apply unless otherwise noted on a quote.

 
PR1MA™ qMAX™ Probe qPCR Mix

• Same high efficiency for multiplex and singleplex reactions
• Includes PR1MA Hot Start Taq Polymerase for greater specificity and accuracy
• Compatible with popular hydrolysis and beacon probes
• Ready to use 2X mastermix
• Early Ct values and detection across a broad dynamic range

Optimized for use with TaqMan™, Scorpions^® and molecular beacon probes, qMax Probe qPCR Mix is a ready-to-use formulation for real-time quantitative assays. qMax Probe utilizes PR1MA™ Hot Start Taq Polymerase or robust PCR with a variety of templates. A specially formulated buffer provides optimal conditions for both superior polymerase function and probe detection, resulting in earlier Ct values and a broad detection range. Complicated, multiplex reactions can be performed without any loss in performance or decrease in detection. The 2X mix requires little, if any optimization and can be used with both fast and standard protocols.

PR1MA™ qMax Probe qPCR Mix can be used to detect any DNA template, including genomic DNA and cDNA. Available in high, low and no ROX formulations, qMax Probe is compatible with most qPCR instruments.

*Please note these products ship on dry ice.  Appropriate shipping charges apply unless otherwise noted on a quote.

PR1MA™ qMAX One-Step RT-qPCR Kits

• RNA to cDNA to qPCR in one tube
• High purity enzyme formulation for enhanced stability and performance
• PR1MA Hot-Start Taq allows for preparation at room-temperature
• Compatible with all qPCR instruments
• Available for green fluorescence or probe detection

Both One-Step Kits are compatible with standard and fast cycling protocols and provide increased sensitivity, speed and reproducibility for a broad range of samples and targets.  The polymerase mix is available with different levels of ROX reference dye for compatibility with all qPCR instruments.

Two versions of our One-Step qPCR Kits are available:

qMAX™ Green One-Step Kits incorporate our proprietary intercalating dye which exhibits higher fluorescent and lower PCR inhibition than other popular dyes such as SYBR.

qMAX™ Probe One-Step Kits are optimized for use with popular TaqMan, Scorpions, and molecular beacon probes.

*Please note these products ship on dry ice.  Appropriate shipping charges apply unless otherwise noted on a quote.

• RNA to cDNA to qPCR, in one tube
• High purity enzyme formulation for enhanced stability and performance
• Accuris Hot-Start Taq allows for preparation at room-temperature
• Blue dye facilitates pipetting and visualization in plates
• Available for green fluorescence or probe detection
• Multiplex formulation available for multiple target amplification

Accuris qMAX One-Step RT-qPCR kits

• RNA to cDNA to qPCR, in one tube
• High purity enzyme formulation for enhanced stability and performance
• Accuris Hot-Start Taq allows for preparation at room-temperature
• Blue dye facilitates pipetting and visualization in plates
• Available for green fluorescence or probe detection
• Multiplex formulation available for multiple target amplification
• Bulk pricing available for high throughput labs and kit manufacturers contact us for details

Accuris qMAX™ One Step Kits allow for highly sensitive real time RT-qPCR assays to be performed directly from RNA templates. Workflows are simplified with optimized formulations of ready-to-use 2X qPCR master mix and 20X reverse transcriptase.

Optimized buffer includes powerful RNase inhibitors, and an extremely thermostable MMLV-derived reverse transcriptase enables robust first strand cDNA synthesis. Accuris Hot Start Taq uses an antibody mediated hot start mechanism allowing for sample preparation at room temperature. Only after an initial incubation at 95C will the Taq become active, so non-specific amplification is greatly reduced. An inert blue dye is included in the Taq master mix to help simplify pipetting and reduce errors.

Three versions of our One Step qPCR kits are available:

qMAX Green One Step kits incorporate our proprietary intercalating dye which exhibits higher fluorescent and lower PCR inhibition than other popular dyes such as SYBR.

qMAX Probe One Step kits are optimized for use with popular TaqMan, Scorpions, and molecular beacon probes.

qMAX Probe One Step Multiplex kits are specifically developed and optimized for efficient probe-based detection of multiple targets in a single reaction well. The Multiplex formulation is comprised of a 20x reverse transcriptase and 2x PCR Mix preparation ideally suited for complex RNA samples including low-copy number viral RNA commonly used in the clinical and research laboratory. qMAX Probe One Step Multiplex kits have been designed to overcome the many challenges of multiplex RT-PCR, by addressing important factors such as the balance between magnesium chloride and deoxynucleotide concentrations, the relative Taq Polymerase and reverse transcriptase concentration, and the ionic conditions of the core reaction buffer.

All Accuris One-Step Kits are compatible with standard and fast cycling protocols and provide increased sensitivity, speed, and reproducibility for a broad range of samples and targets. The polymerase mix is available with different levels of ROX reference dye for compatibility with all qPCR instruments.

Need a great (q)PCR Regents at a great price? Try PR1MA!  Shop Green, Gold, Probe, or One Step.

New and Improved! Validated for SNP Genotyping assays, Gene expression assays, Microarray validation and High throughput screening!

Bullseye EvaGreen qPCR MasterMix is designed for quantitative/real-time analysis. The components of Bullseye EvaGreen qPCR MasterMix has been developed for superb performance in sensitivity, signal-to-noise ratio, and complete elimination of primer dimers. The chemically modified Hot-Start Taq polymerase, included in our Mastermix, significantly reduces non-specific PCR amplification observed with regular Taq polymerase. Every lot is tested on different real-time PCR machines listed below.

• Instrument-specific & pre-optimized
• Validated applications
• Reproducible results
• Highly competitive pricing

For laboratory research only. Not for clinical applications.

For technical questions, phone the MIDSCI helpline at 800-227-9997

• Inert yellow dye helps reduce pipetting errors
• Room temperature stable for up to 30 days
• Compatible with fast cycling protocols
• Highly sensitive for low copy number templates
• Includes PR1MA Hot Start Taq Polymerase

Successful PCR requires careful control of many variables.  Pipetting errors, poor performing polymerases, dNTP concentrations, are just a few of the variables that can all contribute to reaction problems.  PR1MA has developed their new qMAX™ Gold to control these variables and help you achieve the best possible amplification performance. To reduce the chance of pipetting errors, qMAX™ Gold includes an inert yellow dye so small volumes are easy to visualize in PCR plates.  

qMAX™ Gold is a ready to use, 2X mastermix of PR1MA Hot Start Taq enzyme, dNTPs, a sensitive fluorescent intercalating dye, and optimized reaction buffer.  The Hot Start Taq allows reaction set up at room temperature while the temperature stable formulation guarantees optimal performance even if the mix is left at room temperature for extended periods.

Just add primers and DNA targets to the mix, and then proceed to amplification.  qMAX™ Gold is compatible with all real time thermal cyclers and exhibits high sensitivity with normal or fast 2-step cycling protocols.
 *Please note these products ship on dry ice.  Appropriate shipping charges apply unless otherwise noted on a quote.
 
 

• Lyophilized components to ship without cold chain
• Highly specific detection profile and high priming efficiency
• Rapid detection of COVID-19 and does not detect other related coronavirus strains

Design of our COVID-19 was an intricate process using a dedicated team of expert bioinformaticians. Finding the right sequence is critical for not only assay specificity, but also sensitivity which can be inhibited by primer-dimer formation. These ultimately reduce the assay's ability to detect SARS-CoV-2 accurately. However, the right design will mitigate these risks and ensure a specific, sensitive and accurate test, as we have done.

Our surveillance program is in place to track the mutation of the virus. Our expert bioinformaticians analyze daily published virus sequences to ensure our test maintains a 100% detection profile. Each new mutation presents a risk to any COVID-19 kit detection and false negatives without surveillance programs in place. As of the 17th July 2020 our COVID-19 test has a 100% detection profile with the 42,655 full length, good quality SARS-CoV-2 sequences published on the GISAID-EpiCoV database.

Ensuring your COVID-19 test is stable during transit and storage is vital to prevent reagent degradation. Within our COVID-19 genesig® Real Time PCR assay we provide oasig components that are provided freeze dried which stabilizes the active components. This enables the reagents to be shipped at ambient temperature. This simplifies the logistics of shipping and is cost effective. If you're in a sophisticated laboratory or a mobile field hospital we can supply complete qPCR packages to your door quickly and cheaply via standard shipping methods without the need for dry ice or a cold chain of any sort.

The COVID-19 genesig® Real Time PCR assay was granted Emergency Use Authorization Only by the United States Food and Drug Administration (FDA).