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This brand is being discontinued, please contact us for other options.
Looking for other alternatives?
Visit our PR1MA Polymerase page for more options!
Store at -20°C. For in-vitro laboratory use only
General Description
Bullseye Taq DNA Polymerase is a thermostable recombinant DNA polymerase, which exhibits very high activity in primer extension and other molecular biology applications. The enzyme is isolated from Thermus aquaticus and has a molecular weight of approximately 94 kDa.
Bullseye Taq DNA Polymerase has both a 5'®3' DNA polymerase and a 5'®3' exonuclease activity. The enzyme lacks a 3'®5' exonuclease activity (no proofreading ability). Taq DNA Polymerase leaves an A¢ overhang, which makes the enzyme ideal for TA cloning.
The 10X Reaction Buffer provided does not contain Mg+2. 25 mM MgCl2 is supplied separately.
10X Mg++ Free Standard Buffer
100 mM Tris-HCl pH 8.3, 500 mM KCl.
Unit Definition
One unit is defined as the amount that incorporates 10 nmoles of dNTPs into acid-precipitable form in 30 minutes at 72°C under standard assay conditions.
Storage and Dilution Buffer
Enzyme is supplied in 20 mM Tris-HCl pH 8.3, 100 mM KCl, 0.1 mM EDTA, 1 mM DTT, 0.5% Tween 20, 0.5%NP40, 50% glycerol.
Quality Control
Each lot of Taq DNA Polymerase is tested for contaminating activities, with no trace of endonuclease activity, nicking activity, exonuclease activity or priming activity.
Suggested Protocol using Taq DNA Polymerase
This protocol serves as a guideline only. Optimal reaction conditions may vary and must be individually determined.
n In some applications, MgCl2 is needed for the best results. For this reason, 25 mM MgCl2 is included with the kit.
1. Thaw 10X Mg2+ Free Standard Buffer, dNTP mix, primer solutions. It is important to mix the solutions completely before use to avoid localized concentrations of salts.
2. Prepare a master mix according to Table 1. The master mix typically contains all the components needed for extension except the template DNA.
Table 1. Reaction components (master mix & template DNA)
Component |
Vol./reaction |
Final Conc. |
10X Mg2+ Free Buffer |
5 µl |
1X |
MgCl2, 25 mM |
1- 9 µl |
0.5 4.5 mM |
dNTP mix (12.5 mM of each) |
0.8 µl |
0.2 mM of each dNTP |
Primer A |
Variable |
0.11.0 µM |
Primer B |
Variable |
0.11.0 µM |
Taq DNA Polymerase |
Variable |
1-5 units |
Template DNA |
Variable |
Variable |
Distilled Water |
Variable |
- - - - |
TOTAL volume |
50 µl |
- - - - |
Table 2. MgCl2 concentration in a 50 mL reaction
Final MgCl2 conc. in reaction (mM) |
0.5 |
1.0 |
1.5 |
2.0 |
2.5 |
3.0 |
3.5 |
4.0 |
4.5 |
Volume of 25 mM MgCl2 per rxn (µl): |
1 |
2 |
3 |
4 |
5 |
6 |
7 |
8 |
9 |
Table 2 provides the volume of 25 mM MgCl2 to add to the master mix if a certain MgCl2 concentration is required.
3. Mix the master mix thoroughly and dispense appropriate volumes into reaction tubes. Mix gently, e.g., by pipetting the master mix up and down a few times.
4. Add template DNA to the individual tubes containing the master mix.
5. Program the thermal cycler according to the manufacturers instructions. For maximum yield and specificity, temperatures and cycling times should be optimized for each new template target or primer pair.
6. Place the tubes in the thermal cycler and start the reaction.
Looking for other alternatives?
2x Master Mix Kit (1.5 mM MgCl2)
Bullseye Taq DNA Polymerase Mix is a ready-to-use 2x reaction mix. Simply add primers, template, and water to successfully carry out primer extensions and other molecular biology applications.
Bullseye Taq polymerase, the NH4+ buffer system, dNTPs, and magnesium chloride are conveniently present in the Taq DNA Polymerase Mix. (Inert Red Dye is present in BE180303 only)
Bullseye Taq DNA Polymerase Mix offers several advantages. Set up time is significantly reduced. The chance of contaminating component stocks is eliminated. Reduction of reagent handling steps leads to better reproducibility. Standard tests can be set up with the confidence that results will be consistent every time.
Composition of 2x Taq Master Mix
150 mM Tris-HCl pH 8.5, 40 mM (NH4)2S04, 3 mM MgCl2, 0.2%
Tween 20Ò
4 mM dNTPs
2 units/µL AS ONE Taq polymerase
Inert Red Dye & Stabilizer (BE180303 only)
This brand is being discontinued, please contact us for other options.
Looking for other alternatives?
Visit our PR1MA Polymerase page for more options!
R-Taq DNA Polymerase 5 units/µL
Cat. No. | Units | 10X Ammonium Buffer (MgCl2 15mM) | MgCl2 25 mM |
BE200303 | 500 | 1.5 mL | 1.5 mL |
BE200304 | 1,000 | 2x 1.5 mL | 2x 1.5 mL |
BE200306 | 2,500 | 4x 1.5 mL | 4x 1.5 mL |
General Description
Bullseye R-Taq DNA Polymerase is a thermostable recombinant DNA polymerase, which exhibits very high activity in primer extension and other molecular biology applications. R-Taq contains a red dye which provides easy and quick identification of reactions to which enzyme was added and allows confirmation of complete mixing. The inert dye has no effect on downstream processes. R-Taq is added directly to the reaction mix and is used in the same manner as standard Taq DNA Polymerase.
Bullseye R-Taq DNA Polymerase has both a 5'®3' DNA polymerase and a 5'®3' exonuclease activity. The enzyme lacks a 3'®5' exonuclease activity. R-Taq DNA Polymerase leaves an A-overhang, which makes the enzyme ideal for TA cloning.
- High performance thermostable DNA polymerase
- Red dye identifies tubes which contain enzyme and confirms complete mixing of reagents
- Leaves an A-overhang
Unit Definition
One unit is defined as the amount that incorporates 10 nmoles of dNTPs into acid-precipitable form in 30 minutes at 72°C under standard assay conditions.
Storage Buffer
Enzyme is supplied in 20 mM Tris-HCl pH 8.3, 100 mM KCl, 0.1 mM EDTA, 1 mM DTT, inert dye, 0.5 % Tweenà 20, 0.5% NP40, 50% glycerol.
Component | Vol./reaction | Final Conc. |
10X Ammonium Buffer | 5 µL | 1X |
dNTP mix (12.5 mM each) | 0.8 µL | 0.2 mM each dNTP |
Primer A | Variable | 0.1-0.5 µM |
Primer B | Variable | 0.1-0.5 µM |
R-Taq DNA Pol | 1 mL | 5 units/reaction |
Distilled Water | Variable | - - - - |
Template DNA | Variable | 0.1-0.5 µg/reaction |
TOTAL volume | 50 µL | - - - - |
Table 2. MgCl2 concentration
3. Mix the master mix thoroughly and dispense appropriate volumes into reaction tubes. Mix gently (e.g., by pipetting) the master mix up and down a few times.
4. Add template DNA (0.1-0.5 mg/reaction) to the individual tubes containing the master mix.
5. Program the thermal cycler according to the manufacturer's instructions.
For maximum yield and specificity, temperatures and cycling times should be optimized for each new template target or primer pair.
6. Place the tubes in the thermal cycler and start the reaction.
7. After primer extension, load 5-10 mL of a 50 mL reaction directly on an agarose gel for analysis.
Tween 20 is a registered trademark of ICI Americas, Inc.
Final MgCl2 conc. in reaction (mM) | 1.5 | 2.0 | 2.5 | 3.0 | 3.5 | 4.0 | 4.5 |
Additional volume of 25 mM MgCl2 per reaction (µL): | 0 | 1 | 2 | 3 | 4 | 5 | 6 |
10X Ammonium Reaction Buffer
Tris-HCl pH 8.5, (NH4)2S04, 15 mM MgCl2, 1% Tween 20
Quality Control
Endonuclease, exonuclease and priming activities are not detected after 3 hours incubation of 1 mg of pUC19 plasmid DNA and 0.5 µg EcoR I digested lambda phage DNA at 72°C in the presence of 40 units of R-Taq DNA Polymerase.
Suggested Protocol using R-Taq Polymerase
This protocol serves as a guideline. Optimal reaction conditions such as incubation times, temperatures, and amount of template DNA may vary and must be individually determined.
1. Thaw 10X Ammonium Buffer, dNTP mix, and primer solutions. It is important to mix the solutions completely before use to avoid localized concentrations of salts.
2. Prepare a master mix according to Table 1. The master mix typically contains all the components needed for extension except the template DNA.
The optimal MgCl2 concentration should be determined empirically but, in most cases a concentration of 1.5 mM, as provided in the 1X Ammonium Buffer, produces satisfactory results. Table 2 provides the volume of 25mM MgCl2 to add to the master mix if a higher MgCl2 concentration is required.
Table 1. Reaction components (master mix & template DNA)
This product has been discontinued.
Please visit the New PR1MA™ qMAX Green qPCR Mix with Blue Tracking Dye for more options!
PR1MA™ qMAX™ Gold
- Inert yellow dye helps reduce pipetting errors
- Room temperature stable for up to 30 days
- Compatible with fast cycling protocols
- Highly sensitive for low copy number templates
- Includes PR1MA Hot Start Taq Polymerase
Successful PCR requires careful control of many variables. Pipetting errors, poor performing polymerases, dNTP concentrations, are just a few of the variables that can all contribute to reaction problems. PR1MA has developed their new qMAX™ Gold to control these variables and help you achieve the best possible amplification performance. To reduce the chance of pipetting errors, qMAX™ Gold includes an inert yellow dye so small volumes are easy to visualize in PCR plates.
qMAX™ Gold is a ready to use, 2X mastermix of PR1MA Hot Start Taq enzyme, dNTPs, a sensitive fluorescent intercalating dye, and optimized reaction buffer. The Hot Start Taq allows reaction set up at room temperature while the temperature stable formulation guarantees optimal performance even if the mix is left at room temperature for extended periods.
Just add primers and DNA targets to the mix, and then proceed to amplification. qMAX™ Gold is compatible with all real time thermal cyclers and exhibits high sensitivity with normal or fast 2-step cycling protocols.
*Please note these
products ship on dry ice. Appropriate shipping charges apply unless
otherwise noted on a quote.
This brand is being discontinued and will only be available while supplies last.
Need a great DNA Safe Stain at a great price? Try PR1MA! Click here to order.
- Safest DNA stain by far
- Replaces EthBr in agarose gel electrophoresis
- Detection of DNA and RNA
- Excitation at 290nm & 490 nm
- Emission at 530nm
DNA SafeStain is a new and safe nucleic acid stain for visualization of double-stranded DNA, single-stranded DNA, and RNA in agarose gels. The dyes are developed to replace toxic ethidium bromide (EthBr, a potent mutagen), commonly used in gel electrophoresis for visualization of nucleic acids in agarose gels. DNA SafeStain is non-carcinogenic by the Ames-test. The results are negative in both the mouse marrow chromophilous erthrocyte micronucleus and mouse spermary spermatocyte chromosomal aberration tests.
DNA SafeStain emits green fluorescence when bound to DNA. It has two excitation wavelength peaks when bound to nucleic acid, at 290nm and 490nm and the emission can be detected at 530nm.
Storage:
Store under dark at 4°C or room temperature.
User Instruction:
DNA SafeStain can be used in the exact same way as EtBr in agarose gel electrophoresis. For example, add 100µl to 1 liter of 1X running buffer (1xTAE or 1x TBE), and use this DNA SafeStain containing buffer to make the agarose gel and run the gel.
Special Notes:
1. For best results, dilute the DNA SafeStain 1:10,000 in your gel running buffer and use this dye-containing running buffer to make your agarose gel and to run the gel.
2. As is the case with EtBr; add more DNA SafeStain, if brighter DNA bands are desired. To view the results, any conventional DNA gel viewing light box or gel documentation system should work well with the DNA SafeStain; although, using a LED light instead of a UV light source is preferred due to the harmful nature of UV light.
This Product is For Research Use Only
For the proper disposal of this product, follow University or Company Guidelines.
Cas9 Nuclease
Clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated (Cas) systems provide bacteria and archaea with adaptive immunity against viruses and plasmids by using CRISPR RNAs (crRNAs) to guide the silencing of invading nucleic acids. The CRISPR system consists of a short non-coding guide RNA (sgRNA) made up of a target complementary CRISPR RNA (crRNA) and an auxiliary transactivating crRNA (tracrRNA). The sgRNA guides the Cas9 endonuclease to a specific genomic locus via base pairing between the crRNA sequence and the target sequence, and cleaves the DNA to create a double-strand break. The location of the break is within the target sequence 3 bases from the NGG PAM (Protospacer Adjacent Motif). The PAM sequence, NGG, must follow the targeted region on the opposite strand of the DNA with respect to the region complementary sgRNA sequence (Fig.1).
Intact Genomics Cas9 Nuclease is the purified recombinant Streptococcus pyogenes Cas9 enzyme containing a nuclear localization signal (NLS) at the C-terminal for targeting to the nucleus. This enzyme is designed to perform CRISPR/Cas9-mediated genome editing. The physical purity of this enzyme is â¥98% as assessed by SDS-PAGE with Coomassie® blue staining.
Quality Control
Quality control is performed following the production of each new lot of product to ensure that it meets the quality standards and specifications designated for the product. Each lot is repeatedly compared side-by-side with leading competitors to ensure our products outperform the competitor before product launching.
Cas9 nuclease is free from detectable RNase, Endonuclease (nicking) and non-specific DNase activities.
Product Source
E. coli BL21 (DE3) strain expressing a Cas9 gene from Streptococcus pyogenes with an N-terminal 6xHis tag and C-terminal SV40 nuclear localization signal (NLS).
Contents & Storage
- Cas9 Nuclease
- 10x Cas9 Nuclease Reaction Buffer
Store Cas9 Nuclease and Buffer at -20 °C
Storage Buffer
50 mM Tris-HCl, 50 mM KCl, 1 mM DTT, 0.1 mM EDTA, 50% Glycerol, pH 7.5 @ 25 °C
1x Cas9 Reaction Buffer
20 mM HEPES, 100 mM NaCl, 5 mM MgCl, 0.1 mM EDTA, pH 6.5 @ 25 °C
Functional Testing
Cas9 Nuclease functional testing was done by in vitro DNA cleavage assay with the following protocol which gives more than 95% digestion of the substrate DNA as determined by agarose gel electrophoresis.
1) Set up 30 µl reaction in a microcentrifuge tube on ice with the following combinations.
Target DNA |
x µl (100ng) |
sgRNA |
x µl (4000ng) |
10x Cas9 Reaction Buffer |
3.0 µl |
Cas9 Nuclease |
1.0 µl (160ng) |
Add H2O up to |
30.0 µl |
T4 DNA Ligase
Intact Genomics T4 DNA Ligase catalyzes the formation of a phosphodiester bond between juxtaposed 5'-phosphate and 3'-hydroxyl termini in duplex DNA or RNA. This enzyme joins DNA fragments with either cohesive or blunt termini as well as repair single stranded nicks in duplex DNA, RNA or DNA/RNA hybrids.
Quality Control
Quality control is performed following the production of each new lot of product to ensure that it meets the quality standards and specifications designated for the product. Each lot is repeatedly compared side-by-side with leading competitors to ensure our products outperform the competitor before product launching.
Intact Genomics T4 DNA Ligase displays up to 3-5X higher ligation efficiency than the nearest competitor.
Product Source
E. coli strain expressing a recombinant clone
Quality Control
Quality control is performed following the production of each new lot of product to ensure that it meets the quality standards and specifications designated for the product. Each lot is repeatedly compared side-by-side with leading competitors to ensure our products outperform the competitor before product launching.
Contents & Storage
- T4 DNA Ligase
- 10x T4 DNA Ligase Reaction Buffer (w/o ATP)
- 10 mM ATP
Store all contents at -20 °C.
Storage Buffer
50 mM Tris-HCl, 50 mM KCl, 1 mM DTT, 0.1 mM EDTA, 50% Glycerol, pH 7.5 @ 25 °C
10x T4 DNA Ligase Reaction Buffer (w/o ATP)
500 mM Tris-HCl, 100 mM MgCl, 100 mM DTT, pH 7.5 @ 25 °C
Note
10x T4 DNA ligase buffer does not contain ATP. You need to add ATP separately.
Unit Definition
One Weiss unit is defined as the amount of enzyme required to convert 1 nmol of 32P from pyrophosphate into Norit-absorbance material in 20 minutes under standard assay conditions.
Protocol
- Set up reaction buffer in a microcentrifuge tube on ice. Use a molar ratio of 1:3 vector to insert DNA.
Component |
10 µl Reaction |
Vector DNA |
x µl |
Insert DNA |
x µl |
10 mM ATP |
1.0µl |
10x T4 Ligase Buffer |
1.0µl |
T4 DNA Ligase |
1.0µl |
Add H2O up to |
10.0µl |
- Gently mix the reaction and centrifuge briefly.
- For cohesive ends, incubate 16 °C for overnight or at room temperature for 30 min.
- For blunt ends, incubate 16 °C for overnight or at room temperature for 2 hrs.
- Heat inactivate at 70 °C for 15 min.
- Cool on ice and transform 2 µl of the reaction into 50 µl competent cells.
ig-Fusion Cloning Kit
Intact Genomics propriety ig-Fusion cloning technology is a simple, rapid and highly efficient cloning kit which allows to directly clone any PCR product(s) to any linearized expression vector at any site. The PCR fragments can be generated by Intact Genomics high fidelity Pfu DNA polymerase or other high-fidelity DNA polymerases, with primers having 15 to 18 bases of homology at their linear ends to where the product need to fuse. The linearized vector can be generated by PCR or restriction enzymes. The kit is so robust that multiple DNA fragments can be assembled simultaneously and cloned into one construct in a single reaction step within short times (usually 10-30 min) with more than 95% cloning efficiency.
Benefits
- Clone any insert at any site within any vector
- Restriction enzyme and phosphatase free system
- Joining multiple large fragments at once
- Precise insertion at a desired orientation
- Rapid and high efficiency with > 95% positive clones
Quality Control
Quality control is performed following the production of each new lot of product to ensure that it meets the quality standards and specifications designated for the product. Each lot is repeatedly compared side-by-side with leading competitors to ensure our products outperform the competitor before product launching.
Contents & Storage
- 5x ig-Fusion enzyme premix: -20 °C
- 2x PCR premix: -20 °C
- High efficiency competent cells: -80 °C
- Recovery medium:4 °C or -20 °C
Protocol
1. Linearize the vector by restriction enzyme digestion or inverse PCR and purify the product with spin column.
2. Design PCR primers for the gene of interest with 15 to 20 bp at 5'-extensions that are complementary to the ends of the linearized vector.
3. Amplify the gene of interest with Intact Genomics 2x PCR premix or any other high-fidelity DNA polymerase. Run the PCR product on an agarose gel to determine the integrity of the PCR product.
4. Purify the PCR product with spin column.
5. Set up the ig-Fusion cloning reaction as follows: Insert and vector molar ratio 3:1 produce the highest number of colonies.
Linearized vector |
x µl (50-100 ng) |
Insert |
x µl (50-100 ng) |
5x ig-Fusion enzyme premix |
2.0 µl |
H2O up to |
10.0 µl |
6. Mix the reaction mixture thoroughly.
7. Incubate the reaction mixture at 50 °C for 10-30 min, then place on ice. Number of colonies depend on the incubation time, insert size and number of inserts need to clone.
8. Use 2.0 µl of the reaction mixture and transform into high efficiency ig 10B chemical or electroporation competent cells (included). To get the maximum number of colonies, we recommend to use ig 10B electrocompetent cells (Cat # 1212).
Need a great Reverse Transcriptase product at a great price? Try PR1MA!
RT 2X Master Mix
- Up to 9kb cDNA synthesis
- Ensures sample to sample consistency
- Large RNA sample volume capacity
- Ready to use
Size: 100 rxns
RT 2X Master Mix is a proprietary, ready-to-use master mix for first-strand cDNA synthesis in a 2X concentration. This optimized reaction mix contains ribonuclease inhibitor, dNTPs, and a balanced concentration for oligo(dT) and random primers. The ribonuclease inhibitor effectively protects RNA template from degradation. The oligo(dT) anneals selectively to the poly(A) tail of mRNAs and the random primers do not require the presence of poly(A) and they are utilized for the transcription of mRNA 5-end regions. The resultant cDNA can be directly used as template in different PCR experiments.
Kit Components
EasyScript R Tase (200U / uL) | 100 L |
2X Reaction Mix | 1200 uL |
Nuclease-Free H2O | 2 x 1 mL |
Storage
- Store at -20°C in a frost-free freezer.
Item # | Description | Quantity | Rxn |
BERTCDNA-25 | RT 2X Master Mix | 250 uL | 25 rxns |
BERTCDNA-100 | RT 2X Master Mix | 1 mL | 100 rxns |
Application
- cDNA synthesis
- Construction of cDNA libraries
- Generation of probes for hybridization
Protocol
- Thaw RNA templates and all reagents on ice. Mix each solution by vortexing.
- Assemble the following components in a tube on ice, and mix well:
Components | Volume | Final Conc. |
Total RNA, or | Variable | 1 ng - 2 ug/rxn |
mRNA | Variable | 1 pg - 2 ng/rxn |
2X Reaction Mix | 10 uL | 1X |
H2O | Up to 19 uL | - |
- Heat the mixture at 65°C for 5 mins and incubate on ice for at least 1 min.
- Collect all components by a brief centrifugation and add 1 µl of the EasyScript RTase to the tube.
- Mix well and collect all the components by a brief centrifugation.
- Incubate the tube at room temperature for 10 min for annealing.
- Perform cDNA synthesis by incubating the tube for 50 min at 42°C.
- Stop the reaction by heating it at 85°C for 5 min.
- Chill on ice. The newly synthesized first-strand cDNA is ready for immediate downstream applications.
EasyScript cDNA Synthesis Kit
Order# |
Description |
Quantity |
Rxn |
G233 |
EasyScript cDNA Synthesis Kit |
5000U (25uL) |
25 rxns |
G234 |
EasyScript cDNA Synthesis Kit |
20,000U (100uL) |
100 rxns |
Application:
- First strand cDNA synthesis for PCR
- Construction of cDNA libraries
- Generation of probes for hybridization
EasyScript cDNA Synthesis Kit is a complete system for the efficient synthesis of first strand cDNA from RNA templates. The recombinant RNasin Ribonuclease Inhibitor, supplied with the kit effectively protects RNA template from degradation.
The kit is also supplied with both oligo(dT) and random primers. The oligo(dT) anneals selectively on the poly(A) tail of mRNA. Random primers do not require the presence of poly(A). Therefore, they can be used for transcription of the 5'-end regions of mRNA. Gene-specific primers may also be used with the kit. The first strand of cDNA can be directly used as a template in PCR.
EasyScript Reverse Transcriptase (RTase) within the kit is a genetically modified form of Moloney Murine Leukemia Virus Reverse Transcriptase (M-MLV). Both the EasyScript and EasyScript Plus are RNase H deficient (negative). The enzyme is purified from bacteria host as a single holoenzyme of 71 kDa with the capacity of first-strand cDNA synthesis of template up to 9kb and can be used for routine cDNA synthesis.
Kit Components:
Components |
EasyScript cDNA Synthesis Kit |
|
|
G233 |
G234 |
EasyScriptRTase (200U/µl) |
5,000U |
20,000U |
Oligo(dT) (10µM) |
40 µl |
160 µl |
Random Primers (10µM) |
40 µl |
160 µl |
5x RT buffer |
150 µl |
600 µl |
RNasin (40U/µl) |
15 µl |
60 µl |
dNTP (10mM) |
40 µl |
160 µl |
RNase-free H2O |
1 ml |
2x1 ml |
Size |
25 rxns |
100 rxns |
Storage Buffer:
50mM Tris-HCl (pH 8.3), 100 mM NaCl, 0.1 mM EDTA, 5 mM DTT, 0.1% (v/v) Triton X-100, and 50% (v/v) glycerol.
Storage:
Store at -20°C in a frost-free freezer. Multiple freezing and thawing of RNA should be avoided. Keep RNA on ice all the time. It is recommended that the first strand cDNA synthesis is carried out under conditions where RNase contamination has been eliminated.
General Protocol:
RT-PCR reactions should be assembled in a RNA-free environment. The use of "clean", automatic pipettes designated for PCR and aerosol resistant barrier tips are recommended.
1. Thaw template RNA and all reagents on ice. Mix each solution by vortexing, and centrifuge briefly to collect residual liquid from the sides of the tubes.
2. Prepare the following reaction mixture in a PCR tube on ice:
|
Volume |
Concentration (final 20µl) |
Total RNA, or poly(A)+RNA |
Variable |
0.5-5µg per reaction |
|
|
50ng-0.5µg per reaction |
Oligo(dT) (10µM) |
1µl |
0.5µM |
or Random Primer (10µM) |
1µl |
0.5µM |
or Sequence-specific Primer |
Variable |
10-15pM |
dNTP (10mM) |
1µl |
500µM |
5X RT Buffer |
4µl |
1X |
RNasin (40U/µl) |
0.5µl |
20U per reaction |
EasyScript RTase (200U/µl) |
1µl |
200U per reaction |
RNase-free H2O |
Variable |
- |
Final volume |
20µl |
- |
3. Incubate at 25°C for 10 minutes if random primer is used. Omit this step if Oligo(dT) primer or sequence specific primer are used.
4. Incubate the mixture at 42°C for 60 minutes.
5. Stop the reaction by heating at 85°C for 5 minutes.
6. Chill on ice. The newly synthesized first-strand cDNA now can be used directly for PCR amplification.
Notes:
1. Isolation of poly(A)+ RNA from total RNA is not mandatory; however, doing so may improve the yield and purity of the final product.
2. RNA sample must be free of contaminating genomic DNA.
3. Unlike the oligo(dT) priming, which usually requires no optimization, the ratio of a random primer to RNA is critical in terms of the average length of cDNA synthesized in the reaction. Increasing the ratio of random primer/RNA will result in higher yield of shorter (~500 bp) cDNA, whereas decreasing this ratio will produce longer products.
4. The synthesized cDNA should be stored at -20°C.
EasyScript Reverse Transcriptase
Order# |
Description |
Quantity |
Rxn |
G231 |
EasyScript Reverse Transcriptase |
5000U (25uL) |
25 rxns |
G232 |
EasyScript Reverse Transcriptase |
20,000U (100uL) |
100 rxns |
Application:
- Synthesis cDNA froma single-stranded RNA or DNA primer extension
- Sequencing dsDNA
- cDNA library
- Template production for use in PCR
- 3'-end labeling of duplex DNA via end-filling reactions
EasyScript Reverse Transcriptase is a genetically modified form of Moloney Murine Leukemia Virus Reverse Transcriptase (M-MLV). Both the EasyScript and EasyScript Plus are RNase H deficient (negative). The enzyme is purified from bacteria h o s t as a single holoenzyme of 71 kDa with the capacity of first-strand cDNA synthesis of template up to 9kb and can be used for routine cDNA synthesis.
Kit Components:
Components |
EasyScript Reverse Transcriptase |
|
|
G231 |
G232 |
EasyScript RTase (200U/µl) |
5,000U |
20,000U |
5x RT buffer |
150 µl |
600 µl |
Size |
25 rxns |
100 rxns |
Storage Buffer:
50mM Tris-HCl (pH 8.3), 100 mM NaCl, 0.1 mM EDTA, 5 mM DTT, 0.1% (v/v) Triton X-100, and 50% (v/v) glycerol.
Storage:
Store at -20°C in a frost-free freezer. Multiple freezing and thawing of RNA should be avoided. Keep RNA on ice all the time. It is recommended that the first strand cDNA synthesis is carried out under conditions where RNase contamination has been eliminated.
General Protocol:
RT-PCR reactions should be assembled in a RNA-free environment. The use of "clean", automatic pipettes designated for PCR and aerosol resistant barrier tips are recommended.
1. Thaw template RNA and all reagents on ice. Mix each solution by vortexing, and centrifuge briefly to collect residual liquid from the sides of the tubes.
2. Prepare the following reaction mixture in a PCR tube on ice:
|
Volume |
Concentration (final 20µl) |
Total RNA, or poly(A)+RNA |
Variable |
0.5-5µg per reaction |
|
|
50ng-0.5µg per reaction |
Oligo(dT) (10µM) |
1µl |
0.5µM |
or Random Primer (10µM) |
1µl |
0.5µM |
or Sequence-specific Primer |
Variable |
10-15pM |
dNTP (10mM) |
1µl |
500µM |
5X RT Buffer |
4µl |
1X |
RNasin (40U/µl) |
0.5µl |
20U per reaction |
EasyScript RTase (200U/µl) |
1µl |
200U per reaction |
RNase-free H2O |
Variable |
- |
Final volume |
20µl |
- |
3. Incubate at 25°C for 10 minutes if random primer is used. Omit this step if Oligo(dT) primer or sequence specific primer are used.
4. Incubate the mixture at 42°C for 60 minutes.
5. Stop the reaction by heating at 85°C for 5 minutes.
6. Chill on ice. The newly synthesized first-strand cDNA now can be used directly for PCR amplification.
Notes:
1. Isolation of poly(A)+ RNA from total RNA is not mandatory; however, doing so may improve the yield and purity of the final product.
2. RNA sample must be free of contaminating genomic DNA.
3. Unlike the oligo(dT) priming, which usually requires no optimization, the ratio of a randomprimer to RNA is critical in terms of the average length of cDNA synthesized in the reaction. Increasing the ratio of random primer/RNA will result in higher yield of shorter (~500 bp) cDNA, whereas decreasing this ratio will produce longer products.
4. The synthesized cDNA should be stored at -20°C.
Gel Cutting Tips
- Safely excise bands from gels
- Avoid cross contamination
- One-handed operation for quick and accurate gel cutting
- Safer than using razor blades and won't scratch transilluminators
- 1.1 x 4 mm