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Large Tank Blotter with two cassettes, 20 x 20cm

  • Designed to optimally transfer very large and difficult proteins.
  • Reproducible transfer results thanks to the plate electrodes made
  • of platinum-plated titanium (anode) and stainless steel (cathode).
  • Efficient cooling system: Water circulation system with alumina
  • ceramic wall located at the chamber base for effective heat
  • removal.
  • Safety lid: asymmetrical configuration of electrical contacts
  • prevents mismatching of electrodes.

Item#:
ASLARGETANKBLOT2020

Double sided water cooled vertical system, 16cmW x 14cm


 With its 16 cm wide x 14 cm tall format, the Reflection 1614 large-format, dual-gel vertical electrophoresis unit is great for separating complex protein samples. Run either one or two electrophoresis gels in this vertical gel box that provides both long run lengths and high separation capacity. With Galileo's unique casting base, users are able to both cast and run gels without ever having to unclamp the upper buffer chamber. This allows for safer and more convenient gel handling, especially for those who may be prone to ripping their gels between the two activities.

This vertical gel box includes a water cooling feature for electrophoresis protocols that require lower temperature. This water can be provided by the tap or other temperature controlled source and is easily connected by attaching standard tubing over the 3/8" barbed in/out ports on the device. Users may also facilitate cooling efficiency and uniformity using Galileo's optional alumina backer plates (see accessories below).

System Features

Each Reflection 85-1614 vertical system includes the following components:

  •     Heavy duty upper buffer chamber with cooling port connections
  •     Lower buffer chamber
  •     Interlock safety lid with attached leads
  •     Casting base
  •     Four-pack each of plain and notched glass plates
  •     One acrylic blocker plate (used when only one gel is being run)
  •     Eight 1.5mm thick side spacers
  •     Four combs of your choice

System Accessories

  •     Alumina cooling plate for use with cooling systems. This plate can be sandwiched between the gel and the inner buffer chamber to improve overall temperature uniformity.
  •     Casting base for quick, efficient and leak-proof gel casting.
Item#:
ASDOUBLEWATVERT16
470nm,

UltraSlim® LED Illuminator

  • Safer to use that standard UV transillumiators
    • NO UV hazard to skin and eyes.
    • NO DNA damage inherent to UV - No worries when cutting out bands for elution.
  • Easy for use and gel cutting
    • NO need to wear amber glasses.
  • The THINNEST blue LED illuminator in the world.
    • Strong blue LED for excitation.
    • Uniform: <10% coefficient of variance (CV) in the central viewing area.
    • Compact and Light: 30 mm in height
  • Wide applications other than DNA/RNA gel visualization, such as fluorescent proteins.
    • Sensitive for a wide range of fluorphores: DNA SafeStain, GelGreen, GelRed, EthBr, SYBR® Safe, SYBR Gold, SYBR Green I® & II, SYPRO® Rudy, SYPRO® Orange, Coomassie Fluor Orange stains, and Lumitein Protein Gel Stain.

The UltraSlim® LED Illuminator is designed for viewing stained gels on the laboratory bench, or within gel documentation systems. Safer lighting from the LED source inside the instrument produces blue light with a narrow emission peak centered at approximately 470 nm, which is more effective for the excitation of most nucleic acid and protein stains. Viewing surface is 120mm x 70mm.

 

Item#:
SLB-01
Your Price:
1001.00
Each

Minigel System 7cm x 8 cm & (1) 6 and (1) 10 tooth comb


Perfectly sized for rapid analysis of small analytes like PCR and RE fragments. This unit features a RapidCast gasketed tray for quick in-unit gel casting.

System Description

Horizontal gel electrophoresis minigel system for 7cm x 8cm minigels. Includes gasketed UVT gel tray with fluorescent ruler, and (1) 6 and (1) 10 tooth comb, 1.5 mm thickness. Comb substitutions are available. Four year manufacturer warranty.

The standard 0708 mini gel system includes a RapidCast™ gasketed gel tray for convenient gel casting right in the buffer chamber.

System features

The 0708 complete system includes these features:

  •     Heavy duty lower buffer chamber
  •     Interlock safety lid with attached leads
  •     Gasketed (cast in place) 7cm wide x 8cm long UV-transparent gel tray with built-in fluorescent ruler and 2 comb slots.
  •     One 1.5mm x 6 tooth comb
  •     One 1.5mm x 10 tooth comb
Item#:
ASMINGEL7AND8S

Horizontal Electrophoresis System, 20cmW x 25cm


Horizontal Large Gel Electrophoresis System for 20cmW x 25cm gel. Includes 20cm x 25cm UVT Gel Tray w/ end gates &amp; (1) 16, (1) 24 and (1) 36 Tooth, 1.5 mm thickness. Includes four year warranty.

 The 81-2025 is a medium capacity system. With a maximum run length of 25cm, the unit can provide high resolution of complex analytes. Or, fitted with maximum capacity combs in all of the tray's 6 comb slots, the unit can handle up to 168 samples. The 2025 features the Galileo ExpressCast gel casting system for quick, tape-free gel casting and includes a built-in bubble level and leveling screws for convenient level adjustment. You may choose from a wide variety of comb options to customize your order.

  •     Heavy duty lower buffer chamber
  •     Interlock safety lid with attached leads
  •     End gated 20cm wide x 25cm long ExpressCast™ UV-transparent gel tray with built-in fluorescent ruler and 6 comb slots.
  •     One 1.5mm x 16 tooth combs, one 1.5mm x 24 tooth combs, one 1.5mm x 36 tooth combs

Galileo automatic buffer recirculation reduces detrimental ion and pH gradients in your buffer, encourages even migration of nucleic acids, and is ideal for long running gels. Buffer recirculation can also prevent glyoxalated RNA from dissociating in the presence of ion gradients.

The system is simple and self contained. A trapping system captures hydrogen bubbles created by electrolysis of the buffer at the cathode (-) end of the unit. As these bubbels move up the canted recirculation tube, they carry buffer along with them and provide a gentle and reliable recirculation system. This system is self regulating and no additional tubing or pumps are required.
Item#:
ASHORIZELCTSY20

Semi-Dry Blotting Systems, 20cm x 20cm

Choose Galileo Insight™ semi-dry sytems for rapid and efficient molecular transfer.
Galileo semi-dry electroblotters provide quick and efficient transfer of nucleic acids and proteins from agarose or acrylamide gels. Since only the tranfer "sandwich" of gel, membrane and blotting papers must be kept wet, much less buffer is required than traditional tank transfer systems. Solid plate style electrodes (stainless steel cathode and platinum anode) assure even and complete molecular transfer.

The larger format 2020 system is ideal for transfer of larger gels or simultaneous transfer of multiple smaller gels. System is supplied complete with power leads and a sample pack of our ultra-pure cotton fiber filter paper.
Item#:
ASSEMIELECT2020
ladder, 10-180 kDa, 2x250ul

Bullseye Pre-stained Protein Ladder, 10-180 kDa

  • Ready to use - no boiling necessary
  • Stable at ambient temperature
  • 2 reference bands - red at ~75kDa and green at ~25kDa
  • Use 5-15µl, depending on the size of the wells
  • No freeze-thaw involved
  • Broad range ladder

Application: The Bullseye Pre-stained Protein Ladder is suitable for visualizing proteins during electrophoresis without staining and for monitoring transfer onto membrane.

The Bullseye Pre-stained Protein Ladder contains 10 prestained proteins, covering a wide range of molecular weights from 10 to 180kDa. It is designed for monitoring protein separated during polyacrylamide gel electrophoresis, verification of Western transfer efficiency on membranes and for sizing of proteins. The ladder is supplied in protein gel loading buffer and is ready-to-use. No need to heat, dilute, or add reducing agent before loading. One prominent feature of this protein ladder, is that it remains in the liquid state at the storage temperature; therefore, there is not a freeze-thaw cycle involved, making the ladder very stable.


Contents:
0.1~0.4 mg/ml of each protein in 20 mM Trisphosphate (pH 7.5 at 25°C), 2% SDS, 1 mM reducing reagent, 3.6 M Urea, and stabilizer.

Usage:
For each application, 5-15µl is needed, depending on the size of the wells.

Quality Control:
Tested in polyacrylamide gel electrophoresis and Western Blotting.

Storage: -20 °C.

Note for image:

The apparent molecular weight of each protein (kDa) has been determined by calibration against an unstained protein ladder in each electrophoresis condition.
Item#:
BEPAR
Your Price:
167.00
Each

Autoradiography Cassette Aluminum 5x7

Classic Push-Button Cassettes

  • Constructed From Lightweight Aluminum & Nylon
  • Special Curved Door Gives Exceptional Screen-Film Contact
Item#:
ASCASSETTES

RunBlue gels are more rigid and up to 10 times stronger than conventional precast gels resulting in more robust gels that can be handled with confidence. No tearing - an 8% gel can be thrown into the air and caught without breaking!

  • RunBlue gels are manufactured using a unique method of polymerisation that results in more uniform and more complete polymerisation of the Acrylamide when compared to conventional, chemical based methods. This results in gels that have superior batch to batch reproducibility and practically eliminates exposure to un-polymerised Acrylamide.
  • RunBlue gels incorporate a stacking gel that has been dyed (red for SDS) for the simplest ever sample loading. In addition, there is no comb to remove as the top of the teeth are fixed in place. This not only prevents the teeth from being accidentally torn off during comb removal but that all of the wells are uniform, with the teeth remaining vertical. This prevents sample cross contamination / inability to load desired Vol. The teeth also protrude above the lower front plate of the gel cassette preventing sample flow over into adjacent wells.
  • Runblue gels are available in two different cassette sizes providing compatibility with the most common gel tanks and apparatus.  10x10 cm cassettes are compatible with Invitrogen/Life Technologies equipment and any other equipment designed for this format, such as the Hoeffer and Owl tanks. Our 8x10 cm cassette is designed to fit the Biorad equipment (Mini Protean I, II and II, Tetra etc).
  • The resolving gel on RunBlue gels extends to the very bottom of the cassette which not only provides improved resolution but also there is no protruding gel that requires trimming prior to blotting. There is also no additional tape / covers to remove - simply take the gel from the pack and use.
  • RunBlue gels are supplied in boxes of 10 gels. They are available in a 12 well format, covering a range of concentrations and gradients.

Benefits of RunBlue Precast Gels

  • 2 year shelf life - never throw away another gel
  • Up to 10x stronger - no more torn, ruined gels
  • Load up to 20µl - see everything in your sample
  • Dyed stacking gel - makes gel loading child's play (SDS = Red)
  • No comb to remove - no more broken or bent teeth
  • Full length resolving gel - improved resolution and better blotting
  • 10x10 cm and 8x10 cm cassette - total compatibility with most popular tanks including Invitrogen and Biorad equipment
  • Fast delivery with immediate and expert technical support for total peace of mind
  • Best value gels available - save money!
Item#:
ASPRECASTGBIOR

MAXI Flex Tube Kit

MAXI Flex Tubes combine two modes of action: electro-elution of nucleic acid molecules from polyacrylamide or agarose gels and dialysis or buffer exchange of protein molecules. Flex Tubes allow rapid, secure, simple loading and recovery, with high performance as the most convenient, user friendly, electro-elution and dialysis system on the market.

KIT CONTENTS

  • Flex Tubes 2/10/30/50/ 100 pieces
  • Supporting tray (for electro elution protocol) 1ea. (select kits)
  • Floating rack (for dialysis protocol) 1ea. (select kits)
  • Information and Protocol Manual 1ea.

SPECIFICATIONS

  • Membrane cut-off: 3.5K(11bp), 6-8K(18-24bp), 12-14K(36-42bp), 25K(76bp) or 50K(152bp) MWCO
  • Tube volume: 3ml
  • Dialysis volume: 0.1-3ml
  • Min. sample size for extraction: 20µg
  • Max. gel slice: 2cm x 1cm
  • Membrane ultra-clean, sulfur and heavy metal free. EDTA treated
  • Flex Tube MWCO are in kilo Daltons (K) for proteins and corresponding base pairs (bp) for nucleic acids as indicated in the table below:

kilo Daltons

base pairs

1K

3 bp

3.5K

11bp

6-8K

18-24bp

12-14K

36-42bp

25K

76bp

50K

152bp

 

APPLICATIONS

  • Dialysis, electro-elution or buffer exchange with volumes between 0.1-3ml
  • Preparation of protein samples for MALDI-MS
  • Sample concentration
  • Large-scale protein dialysis, such as antibodies and recombinant protein purification
  • Removal of contaminating micro-molecules
  • Tissue culture extraction purification
  • Removal of salts, surfactants, solvents, and detergents
  • Complex formation studies (protein-protein, protein-DNA, and protein-RNA)
  • pH and buffer adjustment of sample solutions, protein extraction or cell extraction
  • High throughput dialysis
  • Peptide dialysis, as small as 10 amino acids
  • Virus-particles purification
Item#:
ASISOLAION2

MIDI Flex Tube Kit

MIDI Flex Tubes combine two modes of action: electro-elution of nucleic acid molecules from polyacrylamide or agarose gels and dialysis or buffer exchange of protein molecules. Flex Tubes allow rapid, secure, simple loading and recovery, with high performance as the most convenient, user friendly, electro-elution and dialysis system on the market.

KIT CONTENTS

  • Flex Tubes 2/10/30/50/ 100 pieces
  • Supporting tray (for electro elution protocol) 1ea. (select kits)
  • Floating rack (for dialysis protocol) 1ea. (select kits)
  • Information and Protocol Manual 1ea.

SPECIFICATIONS

  • Membrane cut-off: 1K(3bp), 3.5K(11bp) or 6-8K(18-24bp) MWCO
  • Tube volume: 800µl
  • Dialysis volume: 50-800µl
  • Min. sample size for extraction: 0.5µg
  • Max. gel slice: 1cm x 0.5cm
  • Membrane ultra-clean, sulfur and heavy metal free. EDTA treated
  • Flex Tube MWCO are in kilo Daltons (K) for proteins and corresponding base pairs (bp) for nucleic acids as indicated in the table below:

kilo Daltons

base pairs

1K

3 bp

3.5K

11bp

6-8K

18-24bp

12-14K

36-42bp

25K

76bp

50K

152bp

 

APPLICATIONS

  • Dialysis, electro-elution or buffer exchange with volumes between 50-800µl
  • Preparation of protein samples for MALDI-MS
  • Sample concentration
  • Large-scale protein dialysis, such as antibodies and recombinant protein purification
  • Removal of contaminating micro-molecules
  • Tissue culture extraction purification
  • Removal of salts, surfactants, solvents, and detergents
  • Complex formation studies (protein-protein, protein-DNA, and protein-RNA)
  • pH and buffer adjustment of sample solutions, protein extraction or cell extraction
  • High throughput dialysis
  • Peptide dialysis, as small as 10 amino acids
  • Virus-particles purification.
Item#:
ASISOLAION4

MEGA Flex Tube Kit

MEGA Flex Tubes enable user friendly dialysis or buffer exchange of protein molecules with rapid, secure and simple loading along with high performance yield recovery.

KIT CONTENTS

  • Flex Tubes 2/10/30/50/ 100 pieces
  • Floating rack (for dialysis protocol) 1ea. (select kits)
  • Information and Protocol Manual 1ea.

SPECIFICATIONS

  • Membrane cut-off: 1K, 3.5K, 6-8K or 12-14K MWCO
  • Tube volume: 10ml, 15ml or 20ml
  • Dialysis volume: 3-20ml
  • Membrane ultra-clean, sulfur and heavy metal free. EDTA treated
  • Flex Tube MWCO are in kilo Daltons (K) for proteins

APPLICATIONS

  • Dialysis or buffer exchange with volumes between 3-20ml
  • Preparation of protein samples for MALDI-MS
  • Sample concentration
  • Large-scale protein dialysis, such as antibodies and recombinant protein purification
  • Removal of contaminating micro-molecules
  • Tissue culture extraction purification
  • Removal of salts, surfactants, solvents, and detergents
  • Complex formation studies (protein-protein, protein-DNA, and protein-RNA)
  • pH and buffer adjustment of sample solutions, protein extraction or cell extraction
  • High throughput dialysis
  • Peptide dialysis, as small as 10 amino acids
  • Virus-particles purification
Item#:
ASISOLAION3
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