IBI Plant Isolate Total DNA Extraction Reagents (Plant Samples)

Introduction
IBI Plant Isolate provides a quick and easy 3 step CTAB and chloroform based method to isolate total DNA (including genomic, mitochondrial and chloroplast DNA) from a variety of plant species (including algae and cyanobacteria). This unique reagent is able to lyse most common plant samples and plant samples with high a polysaccharide content. The extracted DNA is suitable for routine PCR screening, Real-Time PCR, Southern Blotting, Mapping and RFLP. Phenol extraction is not required and the entire procedure can be completed within 50 minutes.

Quality Control
IBI Plant Isolate is tested on a lot-to-lot. 50 mg of fresh Arabidopsis leaves are initially ground in IBI Plant Isolate. A 15 µL aliquot of extracted genomic DNA from a µL eluate is analyzed by electrophoresis on a 1% agarose gel.

Advantages

• High molecular weight genomic DNA extraction from a variety of plant species 
  
• Sample: up to 1 g of fresh plant tissue and up to 0.5 g of dry plant tissue
  
• Scalable, simple and gentle CTAB and chloroform based DNA precipitation method
  
• Cost effective

  Applications
  PCR, Real-Time PCR, Southern Blotting, Mapping and RFLP

  Caution
  IBI Plant Isolate contains irritants. During operation, always wear a lab coat, disposable gloves, protective goggles and (anti-fog) procedure mask.

  Additional Requirements
  Mortar and pestle, 1.5 mL microcentrifuge tubes or 15 mL centrifuge tubes, absolute ethanol for preparing 70% ethanol in water, chloroform, isopropanol, TE buffer or ddH2O

  Components and Storage

  Item	Product	Volume	RNase A (50mg/mL)	Shipping	Storage
  IBI Plant Isolate & RNase A	IB47610	4 mL	N/A	Room Temperature	Plant Isolate Dry at room temperature (15-25°C) for up to 1 year   RNase A 4°C for extended periods
  	IB47611	100 mL	50 µL
  	IB47612	200 mL	100 µL