The ENDURO Mini is a great saver of space and money. This power supply can handle up to 2 gel boxes at atime and is great for horizontal DNA gels and mini-vertical protein gels. These are small in size but not performance. The ergonomic design of the ENDURO Mini allows them to be stacked. However, to minimize cabling, up to three power supplies can be placed within the PowerStock allowing optimal air circulation while only utilizing one power outlet at the bench. These units are full featured with 300V, 400mA, 60W and built in timer. They can run at either constant voltage or constant current and have automatic cross-over as well as no-load detection.

Output voltage/increments	10-300V/ 1V
Output current/increments	10-400mA/1mA
Output power	60W
Operating constant modes	Constant voltage or constant current
Timer	1-999 minutes with alarm, or continuous
Dimensions (WXDXH)	140 X 191 X 84 mm
Weight	1 kg
Input voltage	100-240V

This product has been discontinued.

Please visit the New PR1MA™ qMAX Green qPCR Mix with Blue Tracking Dye for more options!

 

 

PR1MA™ qMAX™ Gold

• Inert yellow dye helps reduce pipetting errors
• Room temperature stable for up to 30 days
• Compatible with fast cycling protocols
• Highly sensitive for low copy number templates
• Includes PR1MA Hot Start Taq Polymerase

Successful PCR requires careful control of many variables.  Pipetting errors, poor performing polymerases, dNTP concentrations, are just a few of the variables that can all contribute to reaction problems.  PR1MA has developed their new qMAX™ Gold to control these variables and help you achieve the best possible amplification performance. To reduce the chance of pipetting errors, qMAX™ Gold includes an inert yellow dye so small volumes are easy to visualize in PCR plates.  

qMAX™ Gold is a ready to use, 2X mastermix of PR1MA Hot Start Taq enzyme, dNTPs, a sensitive fluorescent intercalating dye, and optimized reaction buffer.  The Hot Start Taq allows reaction set up at room temperature while the temperature stable formulation guarantees optimal performance even if the mix is left at room temperature for extended periods.

Just add primers and DNA targets to the mix, and then proceed to amplification.  qMAX™ Gold is compatible with all real time thermal cyclers and exhibits high sensitivity with normal or fast 2-step cycling protocols.
 *Please note these products ship on dry ice.  Appropriate shipping charges apply unless otherwise noted on a quote.
 
 

Double sided non cooled vertical system, 20cmW x 10cm

 The Galileo Bioscience Reflection 2010 is a wide, high capacity gel electrophoresis separation system that allows users to run up to two protein gels simultaneously on a 20 cm wide x 10 cm tall format.  The large gel format makes this unit ideal for users who have a large number of protein samples to run simultaneously.  Galileo's unique casting base allows users to both cast and run gels without ever having to unclamp the upper buffer chamber.  This makes handling gels safer and more convenient, especially for those who may be prone to ripping their gels between the two activities.

The 2010 vertical gel box also includes a water cooling feature for electrophoresis protocols that require lower temperature.  This water can be provided by the tap or other temperature controlled source and is easily connected by attaching standard tubing over the 3/8" barbed in/out ports on the device.  Users may also facilitate cooling efficiency and uniformity using Galileo's optional alumina backer plates (see accessories below).

System Features

Each Reflection 85-2010-V vertical system includes the following features:

•     Heavy duty upper buffer chamber with cooling port connections
•     Lower buffer chamber
•     Interlock safety lid with attached leads
•     Casting base
•     Four-pack each of plain and notched glass plates
•     One acrylic blocker plate (used when only one gel is being run)
•     Eight 0.8mm thick side spacers
•     Four combs of your choice

System Accessories

•     Alumina cooling plate for use with cooling systems. This plate can be sandwiched between the gel and the inner buffer chamber to improve overall temperature uniformity.
•     Casting base for quick, efficient and leak-proof gel casting.

Cas9 Nuclease 
Clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated (Cas) systems provide bacteria and archaea with adaptive immunity against viruses and plasmids by using CRISPR RNAs (crRNAs) to guide the silencing of invading nucleic acids. The CRISPR system consists of a short non-coding guide RNA (sgRNA) made up of a target complementary CRISPR RNA (crRNA) and an auxiliary transactivating crRNA (tracrRNA). The sgRNA guides the Cas9 endonuclease to a specific genomic locus via base pairing between the crRNA sequence and the target sequence, and cleaves the DNA to create a double-strand break. The location of the break is within the target sequence 3 bases from the NGG PAM (Protospacer Adjacent Motif). The PAM sequence, NGG, must follow the targeted region on the opposite strand of the DNA with respect to the region complementary sgRNA sequence (Fig.1).

Intact Genomics Cas9 Nuclease is the purified recombinant Streptococcus pyogenes Cas9 enzyme containing a nuclear localization signal (NLS) at the C-terminal for targeting to the nucleus. This enzyme is designed to perform CRISPR/Cas9-mediated genome editing. The physical purity of this enzyme is â¥98% as assessed by SDS-PAGE with Coomassie® blue staining.

Quality Control 
Quality control is performed following the production of each new lot of product to ensure that it meets the quality standards and specifications designated for the product. Each lot is repeatedly compared side-by-side with leading competitors to ensure our products outperform the competitor before product launching. 

Cas9 nuclease is free from detectable RNase, Endonuclease (nicking) and non-specific DNase activities.

Product Source 
E. coli BL21 (DE3) strain expressing a Cas9 gene from Streptococcus pyogenes with an N-terminal 6xHis tag and C-terminal SV40 nuclear localization signal (NLS).

Contents & Storage

1. Cas9 Nuclease
2. 10x Cas9 Nuclease Reaction Buffer

Store Cas9 Nuclease and Buffer at -20 °C

Storage Buffer 
50 mM Tris-HCl, 50 mM KCl, 1 mM DTT, 0.1 mM EDTA, 50% Glycerol, pH 7.5 @ 25 °C

1x Cas9 Reaction Buffer 
20 mM HEPES, 100 mM NaCl, 5 mM MgCl, 0.1 mM EDTA, pH 6.5 @ 25 °C

Functional Testing
Cas9 Nuclease functional testing was done by in vitro DNA cleavage assay with the following protocol which gives more than 95% digestion of the substrate DNA as determined by agarose gel electrophoresis.
1) Set up 30 µl reaction in a microcentrifuge tube on ice with the following combinations.

Target DNA	x µl (100ng)
sgRNA	x µl (4000ng)
10x Cas9 Reaction Buffer	3.0 µl
Cas9 Nuclease	1.0 µl (160ng)
Add H2O up to	30.0 µl

T4 DNA Ligase

Intact Genomics T4 DNA Ligase catalyzes the formation of a phosphodiester bond between juxtaposed 5'-phosphate and 3'-hydroxyl termini in duplex DNA or RNA.  This enzyme joins DNA fragments with either cohesive or blunt termini as well as repair single stranded nicks in duplex DNA, RNA or DNA/RNA hybrids.
 

Quality Control 
Quality control is performed following the production of each new lot of product to ensure that it meets the quality standards and specifications designated for the product. Each lot is repeatedly compared side-by-side with leading competitors to ensure our products outperform the competitor before product launching. 

Intact Genomics T4 DNA Ligase displays up to 3-5X higher ligation efficiency than the nearest competitor.

Product Source
E. coli strain expressing a recombinant clone

Quality Control 
Quality control is performed following the production of each new lot of product to ensure that it meets the quality standards and specifications designated for the product. Each lot is repeatedly compared side-by-side with leading competitors to ensure our products outperform the competitor before product launching. 

Contents & Storage

1. T4 DNA Ligase
2. 10x T4 DNA Ligase Reaction Buffer (w/o ATP)
3. 10 mM ATP

Store all contents at -20 °C.

Storage Buffer 
50 mM Tris-HCl, 50 mM KCl, 1 mM DTT, 0.1 mM EDTA, 50% Glycerol, pH 7.5 @ 25 °C

10x T4 DNA Ligase Reaction Buffer (w/o ATP) 
500 mM Tris-HCl, 100 mM MgCl, 100  mM DTT, pH 7.5 @ 25 °C

Note
10x T4 DNA ligase buffer does not contain ATP. You need to add ATP separately.

Unit Definition 
One Weiss unit is defined as the amount of enzyme required to convert 1 nmol of 32P from pyrophosphate into Norit-absorbance material in 20 minutes under standard assay conditions.

Protocol

1. Set up reaction buffer in a microcentrifuge tube on ice. Use a molar ratio of 1:3 vector to insert DNA.

  

Component	10 µl Reaction
Vector DNA	x µl
Insert DNA	x µl
10 mM ATP	1.0µl
10x T4 Ligase Buffer	1.0µl
T4 DNA Ligase	1.0µl
Add H2O up to	10.0µl

  

1. Gently mix the reaction and centrifuge briefly.
2. For cohesive ends, incubate 16 °C for overnight or at room temperature for 30 min.
3. For blunt ends, incubate 16 °C for overnight or at room temperature for 2 hrs.
4. Heat inactivate at 70 °C for 15 min.
5. Cool on ice and transform 2 µl of the reaction into 50 µl competent cells.

ig-Fusion Cloning Kit 
Intact Genomics propriety ig-Fusion cloning technology is a simple, rapid and highly efficient cloning kit which allows to directly clone any PCR product(s) to any linearized expression vector at any site. The PCR fragments can be generated by Intact Genomics high fidelity Pfu DNA polymerase or other high-fidelity DNA polymerases, with primers having 15 to 18 bases of homology at their linear ends to where the product need to fuse. The linearized vector can be generated by PCR or restriction enzymes. The kit is so robust that multiple DNA fragments can be assembled simultaneously and cloned into one construct in a single reaction step within short times (usually 10-30 min) with more than 95% cloning efficiency.

Benefits

• Clone any insert at any site within any vector
• Restriction enzyme and phosphatase free system
• Joining multiple large fragments at once
• Precise insertion at a desired orientation
• Rapid and high efficiency with > 95% positive clones

Quality Control 
Quality control is performed following the production of each new lot of product to ensure that it meets the quality standards and specifications designated for the product. Each lot is repeatedly compared side-by-side with leading competitors to ensure our products outperform the competitor before product launching. 

Contents & Storage

• 5x ig-Fusion enzyme premix: -20 °C
• 2x PCR premix: -20 °C
• High efficiency competent cells: -80 °C
• Recovery medium:4 °C or -20 °C

Protocol
1. Linearize the vector by restriction enzyme digestion or inverse PCR and purify the product with spin column.
2. Design PCR primers for the gene of interest with 15 to 20 bp at 5'-extensions that are complementary to the ends of the linearized vector.
3. Amplify the gene of interest with Intact Genomics 2x PCR premix or any other high-fidelity DNA polymerase. Run the PCR product on an agarose gel to determine the integrity of the PCR product.
4. Purify the PCR product with spin column.
5. Set up the ig-Fusion cloning reaction as follows: Insert and vector molar ratio 3:1 produce the highest number of colonies.  

Linearized vector	x µl (50-100 ng)
Insert	x µl (50-100 ng)
5x ig-Fusion enzyme premix	2.0 µl
H2O up to	10.0 µl

6.  Mix the reaction mixture thoroughly.
7.  Incubate the reaction mixture at 50 °C for 10-30 min, then place on ice. Number of colonies depend on the incubation time, insert size and number of inserts need to clone.
8.  Use 2.0 µl of the reaction mixture and transform into high efficiency ig 10B chemical or electroporation competent cells (included). To get the maximum number of colonies, we recommend to use ig 10B electrocompetent cells (Cat # 1212).

RT 2X Master Mix

• Up to 9kb cDNA synthesis
• Ensures sample to sample consistency
• Large RNA sample volume capacity
• Ready to use

Size: 100 rxns

RT 2X Master Mix is a proprietary, ready-to-use master mix for first-strand cDNA synthesis in a 2X concentration. This optimized reaction mix contains ribonuclease inhibitor, dNTPs, and a balanced concentration for oligo(dT) and random primers. The ribonuclease inhibitor effectively protects RNA template from degradation. The oligo(dT) anneals selectively to the poly(A) tail of mRNAs and the random primers do not require the presence of poly(A) and they are utilized for the transcription of mRNA 5-end regions. The resultant cDNA can be directly used as template in different PCR experiments.

Kit Components

EasyScript R Tase (200U / uL)	100 L
2X Reaction Mix	1200 uL
Nuclease-Free H2O	2 x 1 mL

Storage

• Store at -20°C in a frost-free freezer.

Item #	Description	Quantity	Rxn
BERTCDNA-25	RT 2X Master Mix	250 uL	25 rxns
BERTCDNA-100	RT 2X Master Mix	1 mL	100 rxns

Application

• cDNA synthesis
• Construction of cDNA libraries
• Generation of probes for hybridization 

Protocol

1. Thaw RNA templates and all reagents on ice. Mix each solution by vortexing.
2. Assemble the following components in a tube on ice, and mix well:

Components	Volume	Final Conc.
Total RNA, or	Variable	1 ng - 2 ug/rxn
mRNA	Variable	1 pg - 2 ng/rxn
2X Reaction Mix	10 uL	1X
H2O	Up to 19 uL	-

1. Heat the mixture at 65°C for 5 mins and incubate on ice for at least 1 min.
2. Collect all components by a brief centrifugation and add 1 µl of the EasyScript RTase to the tube.
3. Mix well and collect all the components by a brief centrifugation.
4. Incubate the tube at room temperature for 10 min for annealing.
5. Perform cDNA synthesis by incubating the tube for 50 min at 42°C.
6. Stop the reaction by heating it at 85°C for 5 min.
7. Chill on ice. The newly synthesized first-strand cDNA is ready for immediate downstream applications. 

EasyScript cDNA Synthesis Kit

Order#	Description	Quantity	Rxn
G233	EasyScript cDNA Synthesis Kit	5000U (25uL)	25 rxns
G234	EasyScript cDNA Synthesis Kit	20,000U (100uL)	100 rxns

Application:

• First strand cDNA synthesis for PCR
• Construction of cDNA libraries
• Generation of probes for hybridization

EasyScript cDNA Synthesis Kit is a complete system for the efficient synthesis of first strand cDNA from RNA templates. The recombinant RNasin Ribonuclease Inhibitor, supplied with the kit effectively protects RNA template from degradation.

The kit is also supplied with both oligo(dT) and random primers. The oligo(dT) anneals selectively on the poly(A) tail of mRNA. Random primers do not require the presence of poly(A). Therefore, they can be used for transcription of the 5'-end regions of mRNA. Gene-specific primers may also be used with the kit. The first strand of cDNA can be directly used as a template in PCR.

EasyScript Reverse Transcriptase (RTase) within the kit is a genetically modified form of Moloney Murine Leukemia Virus Reverse Transcriptase (M-MLV). Both the EasyScript and EasyScript Plus are RNase H deficient (negative). The enzyme is purified from bacteria host as a single holoenzyme of 71 kDa with the capacity of first-strand cDNA synthesis of template up to 9kb and can be used for routine cDNA synthesis.

Kit Components:

Components	EasyScript cDNA Synthesis Kit
	G233	G234
EasyScriptRTase (200U/µl)	5,000U	20,000U
Oligo(dT) (10µM)	40 µl	160 µl
Random Primers (10µM)	40 µl	160 µl
5x RT buffer	150 µl	600 µl
RNasin (40U/µl)	15 µl	60 µl
dNTP (10mM)	40 µl	160 µl
RNase-free H2O	1 ml	2x1 ml
Size	25 rxns	100 rxns

Storage Buffer:
50mM Tris-HCl (pH 8.3), 100 mM NaCl, 0.1 mM EDTA, 5 mM DTT, 0.1% (v/v) Triton X-100, and 50% (v/v) glycerol.

Storage: 
Store at -20°C in a frost-free freezer. Multiple freezing and thawing of RNA should be avoided. Keep RNA on ice all the time. It is recommended that the first strand cDNA synthesis is carried out under conditions where RNase contamination has been eliminated.

General Protocol:

RT-PCR reactions should be assembled in a RNA-free environment. The use of "clean", automatic pipettes designated for PCR and aerosol resistant barrier tips are recommended. 
1. Thaw template RNA and all reagents on ice. Mix each solution by vortexing, and centrifuge briefly to collect residual liquid from the sides of the tubes.
2. Prepare the following reaction mixture in a PCR tube on ice:

	Volume	Concentration (final 20µl)
Total RNA, or poly(A)+RNA	Variable	0.5-5µg per reaction
		50ng-0.5µg per reaction
Oligo(dT) (10µM)	1µl	0.5µM
or Random Primer (10µM)	1µl	0.5µM
or Sequence-specific Primer	Variable	10-15pM
dNTP (10mM)	1µl	500µM
5X RT Buffer	4µl	1X
RNasin (40U/µl)	0.5µl	20U per reaction
EasyScript RTase (200U/µl)	1µl	200U per reaction
RNase-free H2O	Variable	-
Final volume	20µl	-

3. Incubate at 25°C for 10 minutes if random primer is used. Omit this step if Oligo(dT) primer or sequence specific primer are used.
4. Incubate the mixture at 42°C for 60 minutes.
5. Stop the reaction by heating at 85°C for 5 minutes.
6. Chill on ice. The newly synthesized first-strand cDNA now can be used directly for PCR amplification.

Notes:
1. Isolation of poly(A)+ RNA from total RNA is not mandatory; however, doing so may improve the yield and purity of the final product.
2. RNA sample must be free of contaminating genomic DNA.
3. Unlike the oligo(dT) priming, which usually requires no optimization, the ratio of a random primer to RNA is critical in terms of the average length of cDNA synthesized in the reaction. Increasing the ratio of random primer/RNA will result in higher yield of shorter (~500 bp) cDNA, whereas decreasing this ratio will produce longer products.

4. The synthesized cDNA should be stored at -20°C.

Click here for PCR or here for qPCR to order.

EasyScript Reverse Transcriptase

Order#	Description	Quantity	Rxn
G231	EasyScript Reverse Transcriptase	5000U (25uL)	25 rxns
G232	EasyScript Reverse Transcriptase	20,000U (100uL)	100 rxns

Application:

• Synthesis cDNA froma single-stranded RNA or DNA primer extension
• Sequencing dsDNA
• cDNA library
• Template production for use in PCR
• 3'-end labeling of duplex DNA via end-filling reactions

EasyScript Reverse Transcriptase is a genetically modified form of Moloney Murine Leukemia Virus Reverse Transcriptase (M-MLV). Both the EasyScript and EasyScript Plus are RNase H deficient (negative). The enzyme is purified from bacteria h o s t as a single holoenzyme of 71 kDa with the capacity of first-strand cDNA synthesis of template up to 9kb and can be used for routine cDNA synthesis.

Kit Components:

Components	EasyScript Reverse Transcriptase
	G231	G232
EasyScript RTase (200U/µl)	5,000U	20,000U
5x RT buffer	150 µl	600 µl
Size	25 rxns	100 rxns

Storage Buffer:
50mM Tris-HCl (pH 8.3), 100 mM NaCl, 0.1 mM EDTA, 5 mM DTT, 0.1% (v/v) Triton X-100, and 50% (v/v) glycerol.

Storage: 
Store at -20°C in a frost-free freezer. Multiple freezing and thawing of RNA should be avoided. Keep RNA on ice all the time. It is recommended that the first strand cDNA synthesis is carried out under conditions where RNase contamination has been eliminated.

General Protocol:

RT-PCR reactions should be assembled in a RNA-free environment. The use of "clean", automatic pipettes designated for PCR and aerosol resistant barrier tips are recommended.

1. Thaw template RNA and all reagents on ice. Mix each solution by vortexing, and centrifuge briefly to collect residual liquid from the sides of the tubes. 
2. Prepare the following reaction mixture in a PCR tube on ice:

	Volume	Concentration (final 20µl)
Total RNA, or poly(A)+RNA	Variable	0.5-5µg per reaction
		50ng-0.5µg per reaction
Oligo(dT) (10µM)	1µl	0.5µM
or Random Primer (10µM)	1µl	0.5µM
or Sequence-specific Primer	Variable	10-15pM
dNTP (10mM)	1µl	500µM
5X RT Buffer	4µl	1X
RNasin (40U/µl)	0.5µl	20U per reaction
EasyScript RTase (200U/µl)	1µl	200U per reaction
RNase-free H2O	Variable	-
Final volume	20µl	-

3. Incubate at 25°C for 10 minutes if random primer is used. Omit this step if Oligo(dT) primer or sequence specific primer are used.
4. Incubate the mixture at 42°C for 60 minutes.
5. Stop the reaction by heating at 85°C for 5 minutes. 
6. Chill on ice. The newly synthesized first-strand cDNA now can be used directly for PCR amplification.

Notes:
1. Isolation of poly(A)+ RNA from total RNA is not mandatory; however, doing so may improve the yield and purity of the final product. 
2. RNA sample must be free of contaminating genomic DNA.
3. Unlike the oligo(dT) priming, which usually requires no optimization, the ratio of a randomprimer to RNA is critical in terms of the average length of cDNA synthesized in the reaction. Increasing the ratio of random primer/RNA will result in higher yield of shorter (~500 bp) cDNA, whereas decreasing this ratio will produce longer products.

4. The synthesized cDNA should be stored at -20°C.

ENDURO VE20 Vertical Gel Electrophoresis System

Enduro VE20 Vertical Electrophoresis System (20 x 20 cm), includes glass plates, 2 x 24 well combs, cooling coil and casting base

• Large format vertical gel system
• Electroblotting and gel electrophoresis in same universal gel tank
• Quick assembly system for ease of use
• Complete system allows casting of gels and running of gels at same time increasing efficiency

The ENDURO VE20 is Labnet's new large format vertical gel electrophoresis system. It distinguishes itself in its flexibility of applications and ease of gel casting and set-up requiring only 4 screws to secure 4 gels. The same gel tank can house a large format electroblotting assembly sold separately. This system is ideal for first- and second dimension SDS-PAGE, native, preparative, gradient and high-resolution nucleic acid electrophoresis.

Innovative screw-clamp technology consists of only four screws to secure up to four 20 x 20cm gels. This vertical screw clamp technology distributes pressure evenly along the height of gel rather than in the center to eliminate plate bowing and gel compression, while maintaining a leak-proof seal during casting.

NO TANK TOP ASSEMBLY
A built-in inner buffer chamber within the PAGE insert allows set up to be completed without inclusion of an upper buffer chamber.

CASTING ADVANTAGES

• Dual purpose PAGE insert eliminates time consuming transfer of glass plates between separate casting and gel running components.
• Glass plates with bonded spacers assure clean well formation, correct alignment for leak-free casting and eliminates manually aligning spacers.
• Casting stand design ensures leak-free casting even if glass plates are misaligned.

Specifications:

Plate Dimensions (w x h x t)	20 x 20 x 0.4 cm
Standard Spacer (w x h)	2 x 20 cm
IPG Spacer (w x h)	0.6 x 20 cm
Unit (w x d x h)	30 x 18 x 27 cm
Unit weight	5.5 lbs
Number of gels	4
Total volume inner buffer chamber	640 mL
Total buffer volume for 2 gels	5.3 L
Total buffer volume for 4 gels	4.8 L
Standard run time for SDS-PAGE without coolling	4-5 hr
Standard run time for SDS-PAGE with coolling	3-4 hr

 

Order#	Description
E2020	Enduro VE20 Vertical Electrophoresis System (20 x 20 cm), includes glass plates, 2 x 24 well combs, cooling coil and casting base
E2020-CU	Enduro VE20 Dual Casting System, includes 20 x 20 cm Dual, 2 sets of glass plates, 1 mm think bonded spacers, 2 x 24 sample, 1 mm thick combs, cooling coil, dummy plate; also includes caster and external casting
E2020-IEF	Enduro VE20 IEF Conversion Kit for 18 cm IPG strips and Tube gels
E2020-TB	Enduro VE20 Blotting System, 20 x 20 cm System Including tank and lid, 4 cassettes, 18 fibre pads, cooling coil