IBI High-Speed Mini Plasmid Kit

The High-Speed Mini Plasmid Kit isolates plasmid DNA from 1-5ml cultures using alkaline lysis and RNase treatment, ensuring minimal genomic DNA/RNA contamination.

Additional Details

The High-Speed Mini Plasmid Kits by IBI Scientific are designed to process a sample size of 1-4 mL of bacterial culture, yielding 20-30 µg for high-copy plasmids and 3-10 µg for low-copy plasmids. With an operation time of 30 minutes or less and a binding capacity of up to 30 µg, these kits offer a rapid and efficient solution for plasmid DNA extraction. Check out our basic guide for plasmid kits for more information.

Technical Details

In the presence of a chaotropic salt, the plasmid DNA in the lysate binds to the glass-fiber matrix in the spin column. The contaminants are washed off with an ethanol-based wash buffer. The purified plasmid DNA is eluted by a low salt elution buffer or water. This procedure does not require DNA phenol extraction or alcohol plasmid. Typical yields are 20-30 µg for high-copy numbered plasmids or 3-10 µg for low-copy numbered plasmids. The purified plasmid DNA is ready to use for restriction enzyme digestion, ligation, PCR, or sequencing reactions. The entire procedure can be completed in under 30 minutes.

Quality Control

The quality of the High-Speed Plasmid Mini Kit is tested on a lot-to-lot basis, by isolating plasmid DNA from a 4 ml overnight E. coli (DH5α) culture, containing plasmid p Bluescript (A600>2U/mL). Following the purification process, a yield of more than 20 µg is expected and the ratio of A260/A280 is between 1.7-1.9. The purified plasmid (1 µg) is used in EcoRI digestion and checked by electrophoresis.

Physical Specifications

The I-Blue MINI Plasmid Kit was designed for rapid isolation of plasmid DNA from 1-7 mL of cultured bacterial cells. I-Blue Lysis Buffer (an optional color indicator) is included with the kit in order to prevent common handling errors, ensuring efficient cell lysis and neutralization. A modified alkaline lysis method and RNase treatment are used to obtain clear cell lysate with minimal genomic DNA and RNA contaminants. Typical yields are 20-35 µg for high-copy number plasmid or 3-10 µg for low-copy number plasmid from 4 mL of cultured bacterial cells. DNA phenol extraction or alcohol precipitation is not required and the entire procedure can be completed within 15 minutes. The purified plasmid DNA is ready for use in restriction enzyme digestion, ligation, PCR, and sequencing reactions.

Advantages

• Sample: 1-7 mL of cultured bacterial cells
• Yield: Up to 50 µg of pure plasmid DNA
• Format: Plasmid spin column
• Operation Time: Within 15 minutes
• Elution Volume: 30-100 µL
• Kit Storage: Dry at room temperature (15-25°C) for up to 1 year; PD1 and RNase A mixture should be stored at 2-8°C for up to 6 months

Quality Control
The quality of the I-Blue MINI Plasmid Kit is tested on a lot-to-lot basis by isolating plasmid DNA from a 4 mL overnight E. coli (DH5α) culture containing plasmid pBluescript (A600 > 2 U/mL). Following the purification process, a yield of more than 20 µg is obtained and the A260/A280 ratio is between 1.8-2.0. The purified plasmid DNA (1 µg) is used in EcoRI digestion, and analyzed by electrophoresis.

Kit Components

*For IB47171 and IB47172 add provided RNase A to PD1 Buffer then mix by shaking for a few seconds. Check the box on the bottle. PD1 and RNase A mixture should be stored at 2-8°C for up to 6 months. For IB47170 samples, RNase A was already added to PD1.

**If precipitates have formed in PD2 Buffer, warm the buffer in a 37°C water bath, followed by gentle shaking to dissolve.

***Add absolute ethanol (see the bottle label for volume) to Wash Buffer then mix by shaking for a few seconds. Check the box on the bottle. Be sure and close the bottle tightly after each use to avoid ethanol evaporation.

The Fast Ion Plasmid Maxi Kit (Endotoxin Free) uses pre-packed anion exchange resin columns to purify plasmid DNA from 100-250 ml of bacterial cultures. Modified alkaline lysis method (1) and RNase treatment are used for obtaining clear cell lysate with minimal genomic DNA and RNA contaminants. Once the plasmid DNA has been bound to the column, the contaminants can be washed off using the wash buffer. Finally, the purified plasmid DNA is eluted by a high salt buffer and precipitated with isopropanol for desalting. The entire procedure can be completed in 120 minutes and the obtained high purity plasmid DNA is suitable for transfection, sequencing reactions, PCR and in-vitro transcription.

The 96-Well Genomic DNA Kits are designed for high-throughput purification of total DNA (including genomic, mitochondrial and viral DNA) from whole blood and a variety of animal tissues or cells. This method uses Proteinase K and a chaotropic salt to lyse cells and degrade proteins. DNA in the chaotropic salt is bound by the glass fiber matrix of each well. Once any contaminants have been removed, the purified DNA is eluted by a low salt elution buffer or water. The entire procedure can be completed in 1 hour without phenol extraction or alcohol precipitation. These kits can be used for manual filtration or with robotic handling systems, and purified DNA with approximately 20-30kb is suitable for PCR or other enzymatic reactions.

The Genomic DNA Mini Kit for Plants provide a quick and easy method for purifying total DNA (including genomic DNA, mitochondrial and chloroplast DNA) from plant tissue. Samples are disrupted by both grinding in liquid nitrogen and lysis buffer incubation. The lysate is treated with RNase A to degrade RNA and then filtered to remove cell debris and salt precipitates. In the presence of the binding buffer, coupled with chaotropic salt, genomic DNA in the lysate binds to the glass fiber matrix of the spin column. Contaminants are removed using a Wash Buffer (containing ethanol) and the purified genomic DNA is eluted by a low salt Elution Buffer or TE. The procedure does not require DNA phenol extraction or alcohol precipitation, and can be completed in less than 1 hour. The purified genomic DNA is ready for use in PCR, Real-time PCR, Southern Blotting and RFLP.

The Genomic DNA Mini Kit for Tissue was designed specifically for purifying total DNA (including genomic, mitochondrial and viral DNA) from a variety of animal tissue, paraffin-embedded tissue, buccal swab and amniotic fluid. The provided micropestle can efficiently homogenize tissue samples to shorten the time in the Lysis Step. Proteinase K and chaotropic salt are used to lyse cells and degrade protein, allowing DNA to be easily bound by the glass fiber matrix of the spin column. Once any contaminants have been removed, using a Wash Buffer (containing ethanol), the purified DNA is eluted by a low salt Elution Buffer or TE. The entire procedure can be completed within 1 hour without phenol/chloroform extraction or alcohol precipitation. The expected yield of genomic DNA is up to 50µg and the purified DNA (with approximately 20-30 Kb) is suitable for use in PCR or other enzymatic reactions.

The Genomic DNA Kit for Blood or Cultured Cells provides an efficient method for purifying total DNA (including genomic, mitochondrial and viral DNA) from whole blood, frozen blood, buffy coat, cultured animal/bacterial cells and fungus. Chaotropic salt is used to lyse cells and degrade protein, allowing DNA to bind to the glass fiber matrix of the spin column. Contaminants are removed using a Wash Buffer (containing ethanol) and the purified genomic DNA is eluted by a low salt Elution Buffer or TE. The entire procedure can be completed in 1 hour without phenol/chloroform extraction or alcohol precipitation, with an average DNA yield of 6 µ g from 200 µ l of whole human blood and up to 50µ g of DNA from 200µ l of buffy coat. Purified DNA, with approximately 20-30 Kb, is suitable for use in PCR or other enzymatic reactions.

MagBio HighPrep™ Viral/Bacterial DNA/RNA Kit

, Beads

Double sided water cooled vertical system, 10cm x 10cm

Extra Wide Large Format Gel System, 23.5cmW x 40cm

•     Heavy duty lower buffer chamber
•     Interlock safety lid with attached leads
•     End gated 23cm wide x 40cm long ExpressCast™ UV-transparent gel tray with built-in fluorescent ruler and 12 comb slots.
•     Two 1.5mm x 25 tooth combs
•     Two 1.5mm x 50 tooth combs

MaGELin Mini Protein System

• For 10 cm x 10 cm cast-your-own polyacrylamide gels
• Comes with dual casting system
• 3 sets of glass plates and 2 sets of 0.8 mm thickness spacers.
• Buffer tank, dual gel capture, vented lid and power cords included

The IB94000 MaGELin Mini protein system comes complete with everything you need to pour 10 cm x 10 cm cast-your-own gels. This highly versatile system will also accommodate the IB95000 Western Transfer module so when you’re not running gels the unit can be used as a dedicated Western transfer system.
 

The IB94000 comes complete with:

• Dual gel capture device for running two gels simultaneously.
• Dual gel casting system that allows casting outside of the running unit.
• Three sets of glass plates for pouring and running gels
• Two sets of special 0.8 mm side spacers to seal the gel sandwich during pouring
• Buffer tank
• Vented lid
• Power cords.
• Two 0.8 mm x 12-tooth combs.

The IB94000 utilizes a unique spacer and casting system to make it easy and fast to cast your own gels. The special side spacers are made specifically to accommodate the thickness of each glass slide that comes with the unit. These side spacers will produce a 0.8 mm thick gel. Once the glass plates have been assembled using the side spacers, they can now be inserted into the gel casting unit. This unit accommodates two sets of glass plates simultaneously. The bottom of the gel casting unit contains a very soft gel-like gasket that will seal the bottom of the gel sandwich. Once the gel sandwich is placed inside the casting unit, place the lid of the casting unit on top and press gently to fasten using the included thumb screws. Once fastened the lid will provide the correct amount of down pressure on the gel sandwich to seal the bottom and hold them in place. The polyacrylamide solution can now be introduced through the opening in the casting fixture lid.