Molecular Biology Reagents/Chemicals
MIDSCI gives you ultra-pure, cost-competitive molecular biology reagents and lab chemicals for life science research; often with significant cost savings over Sigma Aldrich and other lab reagent providers.
Kappa Chemicals can help with a wide variety of research-based requirements.
Use our glycerol for polyacrylamide gel electrophoresis. Eliminate Agrobacterium after inoculation with the carbenicillin we carry. Or detect β-Galactosidase activity with X-GAL which, when combined with the IPTG we stock, can be used to detect lac gene expression in cloning applications.
We also carry Luria Broth (Lennox), for the propagation and maintenance of E. coli; DTT, in order to maintain –SH groups in a reduced state; and high gel strength agar for tissue culture and microbiological work.
MIDSCI carries the full line of Kappa General Lab Chemicals. Please call us if you don't see what you are looking for here: 800-227-9997
TBE, TAE, and Fast Running Buffer
QuickSilver - TAE (Tris-acetate-EDTA)
TAE is ideal for separation of large DNA fragments in agarose gels and is used as running buffer and in gel preparation.
TAE is the buffer of choice when recovering DNA from gels and when downstream processing involves enzymatic reactions. Final concentration of a 1X working solution is 40mM Tris, 20mM acetic acid, 1mM EDTA.
QS1000 QuickSilver TAE Buffer, 1X, 100 packs
QuickSilver - TBE (Tris-borate-EDTA)
TBE is used in gel preparation and as a running buffer for electrophoresis of nucleic acids. TBE works best for resolution of smaller fragments (<2kb). Its higher buffering capacity makes it useful for longer runs. The final concentration of a 1X working solution is 89mM Tris, 89mM boric acid, 2mM EDTA.
QS1001 QuickSilver TBE Buffer, 1X, 50 packs
QuickSilver - Fast Running Buffer
QuickSilver Fast Running Buffer is a proprietary blend formulated for agarose gel electrophoresis at high voltage (300-350V). Its strong buffering capacity minimizes heat generation all allows for extremely fast separation of fragments smaller than 5kb. A typical 30-minute run can be performed in just 10 minutes using this buffer.
QS1002 QuickSilver Fast Running Buffer, 1X, 100 packs