Molecular Biology Reagents/Chemicals

MIDSCI™ gives you ultra-pure, cost-competitive molecular biology reagents and lab chemicals for life science research; often with significant cost savings over Sigma Aldrich and other lab reagent providers.

PR1MA™ Chemicals can help with a wide variety of research-based requirements.
 
Use our glycerol for polyacrylamide gel electrophoresis. Eliminate Agrobacterium after inoculation with the carbenicillin we carry. Or detect β-Galactosidase activity with X-GAL which, when combined with the IPTG we stock, can be used to detect lac gene expression in cloning applications.
 
We also carry Luria Broth (Lennox), for the propagation and maintenance of E. coli; DTT, in order to maintain –SH groups in a reduced state; and high gel strength agar for tissue culture and microbiological work.

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PR1MA Agarose SS, Super High Strength, Molecular Biology Grade

CAS Number: 9012-36-6
Solubility: Water
Storage Temperature: Room Temperature


For DNA/RNA fragments >1000 bp. Ideal for separating larger molecules. DNAse/RNase free.


Gel Strength, 1%: >1800 g/cm2
Gel Strength, 1.5%: >3200 g/cm2
Gelling Temperature: 35-37°C
Melting Temperature: 87-89°C
Sulfate: <0.12%
EEO: <0.13%


Research or further manufacturing use only, not for food or drug use.
 

  • Appearance: Fine white homogeneous powder
  • DNase/ RNase: Not Detected
  • Gelling Temperature:  10 oC
  • Melting Point: 80 - 100 oC

Item#:
ASPRIMAAGASHS
[Benzyl Penicillin Sodium Salt]

PR1MA  Penicillin G Sodium Salt [Benzyl Penicillin Sodium Salt]

Common Name: Benzyl Penicillin Sodium Salt
CAS Number: 69-57-8
Molecular Weight: 356.4
Chemical Formula: C16H17N2NaO4S
Solubility: Water
Storage Temperature: 2-8°C


Inhibitor of bacterial cell wall synthesis. Active against gram positive and some gram negative bacteria. β-Lactamase sensitive.


Research or further manufacturing use only, not for food or drug use.


  • Appearance: White crystalline powder
  • Potency : 1500 - 1750 U/mg
  • Assay: 96.0 - 102.0 %
  • Identification: Pass Test
  • Loss on Drying:  1.0 %
  • pH: 5.5 - 7.5
  • Specific Optical Rotation: +285 - +310 °
  • Absorbance 264 nm: 0.80 - 0.88
  • Absorbance 280nm:  0.10
  • Absorbance 325nm:  0.10
  • Related Substances: Conforms to standard
  • Bacterial Endotoxins : Conforms to standard
  • Sterility: Conforms to standard
  • Country of Origin : Report

PR1MA  Neomycin Sulfate

CAS Number: 1405-10-3
Molecular Weight: 908.9
Chemical Formula: C23H46N6O13  3H2SO4
Solubility: Water
Storage Temperature: Room Temperature


Aminoglycoside antibiotic active against most gram negative bacteria. Selective agent for the incorporation of the NPT II (APH3') gene in plant tissue. Plant cell culture tested.
Ingredients:

  • Neomycin B: 85%
  • Neomycin C: 10%

WARNING! This product can expose you to Neomycin sulfate, a chemical which is known to the State of California to cause developmental harm. For more information go to www.P65Warnings.ca.gov.

 
Research or further manufacturing use only, not for food or drug use.
 

  • Appearance: White to Yellowish- White Powder
  • Identification: Pass Test
  • Loss on Drying:  8.0 %
  • pH (3.3 % in Aqueous Solution): 5.0 - 7.5
  • Potency :  600 IU/mg
  • Non Animal Origin: Non Animal Origin
  • Country of Origin: Report

PR1MA Proteinase K

CAS Number: 39450-01-6
Solubility: Water
Storage Temperature: -20°C

From Tritirachium album. Serine protease for proteolytic inactivation of nucleases during isolation of DNA and RNA.

Shipping Weight: 0 lbs
Shipping Dimensions: 1.25 x 1.75 x 1.25


Specifications

Appearance: White to Off White Lyophilized Solid
Solubility: 50mM Tris-HCl (pH 7.5), 3mM CaCl2, 50% Glycerol
Purity: ≥ 98.0 %
DNase/ RNase: Not Detected
Activity: ≥ 30.0 U/mg
Unit Definition: 1 unit is defined as the amount of enzyme that will liberate 1.0 µmol of tyrosine from casein/min @37°C, pH 7.5
Country of Origin: China


Research or further manufacturing use only, not for food or drug use.

PR1MA Malt Extract Agar

CAS Number: 8002-48-0
Solubility: Water with Heat
Storage Temperature: Room Temperature


Malt Extract Agar is used for the isolation, cultivation and maintenance of yeasts and molds. Formulation is similar to Malt Extract Broth with the addition of agar and glycerol. This broth medium was recommended by Thom and Church for the cultivation of fungi. Its low acidity inhibits the growth of most bacteria. The high carbohydrate content enhances the rapid growth of fungi and yeasts.


Dissolve 33.6 grams in 1 liter of distilled water. Sterilize by autoclaving for 15 minutes.


Ingredients:

  • Maltose: 12.75 g/L
  • Dextrin: 2.75 g/L
  • Glycerol: 2.35 g/L
  • Peptone: 0.78 g/L
  • Agar: 15.0 g/L
  • Final pH (25°C): 4.6 ± 0.2



Research or further manufacturing use only, not for food or drug use.


  • DCM Appearance: Homogeneous with soft lumps, free flowing, light beige
  • PPM Appearance: Trace to moderate haze, off white to pale yellow
  • pH(3.36% Solution @ 25 oC: 4.5 - 4.9
  • Microorganism (Aspergillus niger ATCC 16404): Growth
  • Microorganism (Candida albicans ATCC 10231): Growth
  • Microorganism (Penicillium Roquefortii ATCC 10110): Growth
  • Microorganism (Saccharomyces cerevisiae ATCC 9763): Growth
  • Microorganisms (Microsporum Audouinii ATCC 42558): Growth
  • Microorganisms (Microsporum Canis ATCC 36299): Growth
  • Microorganism (Trichophyton Mentagrophytes ATCC 9533): Growth

PR1MA Formamide, Super Pure

CAS Number: 75-12-7
Molecular Weight: 45.04
Chemical Formula: CH3NO
Storage Temperature: 2-8°C

For nucleic acid hybridization and sequencing

Shipping Weight: 0.1 lbs
Shipping Dimensions: 3.38 x 4.63 x 3.38


Specifications
Appearance: Clorless Liquid
Assay: ≥ 99.5 %
Freezing Point: 1.0 - 3.0 °C
Copper: ≤ 0.0001 %
Iron: ≤ 0.0005 %
Lead (Pb): ≤ 0.0001 %
Zinc: ≤ 0.0001 %

Research or further manufacturing use only, not for food or drug use.

PR1MA Sucrose

CAS Number: 57-50-1
Molecular Weight: 342.3
Chemical Formula: C12H22O11
Storage Temperature: Room Temperature

Ultra-high purity, double crystallized to remove any remaining inorganic and organic contamination. Produces a clear colorless solution. Used for cultivating protoplasts and "in vitro" multiplication of plant tissue.

Research or further manufacturing use only, not for food or drug use.

  • Appearance: Fine White Crystals
  • Absorbance 260nm:  0.07 nm
  • Absorbance 280nm:  0.05
  • DNase, RNase, protease: Not Detected
  • Heavy Metals:  0.0005 %
  • Identification (IR): Pass
  • Purity:  99.0 %
  • Solubility (10% solution in H2O @ 25oC): Pass
  • Sulfate:  0.005 %

 

PR1MA Tryptone, Granulated

CAS Number: 91079-40-2
Solubility: Water
Storage Temperature: Room Temperature


Pancreatic digest of casein. Material is specially granulated to reduce dust and increase solubility rate.


Ingredients:

  • Total Nitrogen (TN): ~12.7%
  • Amino Nitrogen (AN): ~3.7%
  • AN/TN Ratio: 0.26
  • Sodium Chloride: ~0.4%
  • Tryptophan: 1.0
  • pH (1% solution): ~7.3%


Research or further manufacturing use only, not for food or drug use.


  • Appearance: Light Beige
  • Amino Nitrogen Content:  3.5 %
  • Ash:  15.0 %
  • Loss on Drying:  5.0 %
  • pH (2.0% Solution @ 25 oC): 6.5 - 7.5
  • Total Nitrogen Content:  10 %
  • Enzyme Species: Porcine
  • Raw Material Species: Bovine

PR1MA Sodium Acetate, Anhydrous

CAS Number: 127-09-3
Molecular Weight: 82.0
Chemical Formula: C2H3O2Na
Solubility: Water
Storage Temperature: Room Temperature


Research or further manufacturing use only, not for food or drug use.


  • Appearance: White Powder
  • Assay: ≥ 99.0 %
  • pH (5% Solution): 7.0 - 9.2
  • Solubility: Soluble in Water
  • Loss on Drying (120 oC):  1.0 %
  • Calcium (Ca):  0.005 %
  • Chloride (Cl):  0.002 %
  • Heavy Metals (as Pb):  0.001 %
  • Insoluble Matter:  0.01 %
  • Iron (Fe):  0.001 %
  • Magnesium (Mg):  0.002 %
  • Phosphate:  0.001 %
  • Sulfate (SO4):  0.003 %
  • Grade: Meet USP/ACS Specifications

PR1MA  Phleomycin 20mg/ml Solution

CAS Number: 11006-33-0
Molecular Weight: 1526.5 g/mol
Chemical Formula: C55H85O21N20S 2Cu  HCl
Solubility: Water
Storage Temperature: -20°C


Supplied at a concentration of 20 mg/ml.


Research or further manufacturing use only, not for food or drug use.
 

  • Appearance: Clear Blue Solution
  • Concentration: = 20 mg/ml
  • Odor: None
  • Solubility (20 mg/ml in Water): Freely soluble
  • Sterile Filtered: Pass test
  • UV Absorbance 244nm (200 ug/ml): 0.75 - 1.05

PR1MA Thiamine Hydrochloride [Vitamin B1]

Common Name: Vitamin B1

CAS Number: 67-03-8

Molecular Weight: 337.3

Chemical Formula: C12H17CIN4OS  HCl

Solubility: Water

Storage Temperature: 2-8°C



Research or further manufacturing use only, not for food or drug use.
 
 

  • Appearance: White Crystalline Powder
  • Appearance of Solution: Clear, no more color than reference solution Y7 or GY7
  • Identification: Should be positive reaction
  • Identification (IR): Consistent with the reference IR spectra
  • pH: 2.7 - 3.3
  • Sulfates:  300 ppm
  • Limit of Nitrate: No brown ring is produced at the junction of the two layers
  • Lead:  2 ppm
  • Water:  5.0 %
  • Residue on Ignition:  0.1 %
  • Absorbance of Solution:  0.025
  • Residual Solvents (Methanol):  0.3 %
  • Residual Solvents (Ethanol):  0.5 %
  • Related Substance (Impurity A):  0.15 %
  • Related Substance (Impurity B):  0.3 %
  • Related Substance (Impurity C):  0.15 %
  • Related Substance (Any Other Impurity):  0.1 %
  • Related Substance (Total impurities):  0.5 %
  • Related Compounds:  1.0 %
  • Chromatographic Purity:  1.0 %
  • Assay: 98.5 - 101.0 %
  • Staphylococcus Aureus: Negative
  • Salmonella: Negative
  • E.Coli: Negative
  • Total Plate Count:  1000 cfu/g
  • Yeast and Mold:  100 cfu/g
  • Material Source: Acrylonitrate; Acetamidine HCl; Chlorine Ester
  • Production Method: Chemical Synthesis
  • BSE/TSE Free: BSE/TSE Free
  • Non Animal Origin: Non Animal Origin
  • Conclusion: This product complies with the specification of BP/USP/FCC.
  • Country of Origin: Report

PR1MA Yeast Extract, Powder

CAS Number: 8013-01-2
Solubility: Water
Storage Temperature: Room Temperature


Dried yeast autolysate with a high content of amino nitrogen and water soluble B complex vitamins. Due to its low NaCl content this quality is well suited for tissue culture.


Ingredients:

  • Total Nitrogen (TN): ~9.8%
  • Amino Nitrogen (AN): ~5.1%
  • AN/TN Ratio: ~0.52
  • Sodium Chloride: ~0.3%
  • pH (1% Solution): ~7.0

Research or further manufacturing use only, not for food or drug use.


  • Appearance: Light Beige Powder
  • Amino Nitrogen: 4.5 - 5.8 %
  • Ash Content: 11.50 - 16.0 %
  • Dry Matter:  95.0 %
  • Loss on Drying:  5.0 %
  • pH (2.0% Solution @ 25 oC): 6.8 - 7.2
  • Sodium Chloride:  0.5 %
  • Total Nitrogen: 10.0 - 11.8 %
  • Clostridium Perfringens Spores : Report cfu/g
  • E.Coli: Report cfu/g
  • Molds and Yeasts: Report cfu/g
  • Pathenogenic Staphylococcus: Report cfu/g
  • Salmonella : Report cfu/25g
  • Standard Plate Count: Report cfu/g
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