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IBI MINI miRNA Isolation

Sample Kit, 4 preps
Item #:IB47370 
25.00
Each
Quantity:
Quantity Web Price
1 25.00
IBI MINI miRNA Isolation

Isolation of micro RNA (miRNA) from Tissue or Cultured Cells

The miRNA Isolation Kits are designed for purification of micro RNA (miRNA) and other small cellular RNAs from tissue samples or cultured cells. Purification of miRNA allows research into biological significant pathways for gene regulation. The standard protocol for isolating total RNA and mRNA are not optimized for isolation of small RNA molecules and result in the loss of substantial amounts of miRNA and other small RNA. 

In addition, removal of the predominantly larger RNAs is required for accurate analysis of miRNA expression by qPCR or microarray analysis. This kit is specifically designed for purification of small RNA with minimal contamination from large RNA molecules or genomic DNA. The method employs a spin column with a silica-based fiber matrix that binds RNA in the presence of a chaotropic salt. The method is based on the selective binding of RNA molecules of various sizes to the silica-based fiber matrix when different ethanol concentrations are present in the solvent.

Sample Types:

100mg tissue or 1 x 10 cultured cells
Format:
Spin column

Operation Time:

30 minutes

PNRNADNA

# of Samples
4
Source Cell
Cultured Cell
Isolation type
RNA
IBI MINI miRNA Isolation
IBI MINI miRNA Isolation
25.00
Each
Quantity: