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PR1MA™ qMAX One-Step RT-qPCR Kits

  • RNA to cDNA to qPCR in one tube
  • High purity enzyme formulation for enhanced stability and performance
  • PR1MA Hot-Start Taq allows for preparation at room-temperature
  • Compatible with all qPCR instruments
  • Available for green fluorescence or probe detection

PR1MA qMAX™ One Step Kits allow for highly sensitive real time RT-qPCR assays to be performed directly from RNA templates.  Workflows are simplified with optimized formulations of ready-to-use 2X qPCR master mix and reverse transcriptase. Optimized buffer includes powerful RNase inhibitors and an extremely thermostable MMLV-derived reverse transcriptase enables robust first strand cDNA synthesis.  PR1MA Hot Start Taq uses an antibody mediated hot start mechanism allowing for sample preparation at room temperature.   Only after an initial incubation at 95°C will the Taq become active, so non-specific amplification is greatly reduced.  An inert blue dye is included in the Taq master mix to help simplify pipetting and reduce errors.


Both One-Step Kits are compatible with standard and fast cycling protocols and provide increased sensitivity, speed and reproducibility for a broad range of samples and targets.  The polymerase mix is available with different levels of ROX reference dye for compatibility with all qPCR instruments.


Two versions of our One-Step qPCR Kits are available:

qMAX Green One-Step Kits incorporate our proprietary intercalating dye which exhibits higher fluorescent and lower PCR inhibition than other popular dyes such as SYBR.

qMAX Probe One-Step Kits are optimized for use with popular TaqMan, Scorpions, and molecular beacon probes.


*Please note these products ship on dry ice.  Appropriate shipping charges apply unless otherwise noted on a quote.

 
PR1MA™ SmartCheck DNA Ladders

  • Ready-to-use formulation includes loading buffer and tracking dye
  • 500 µL suitable for 100 lanes (5 µL per lane)
  • Higher intensity reference bands
  • Ultra pure production allows economical ambient shipping

 

Technical Details
 

Protocol: Briefly vortex the tube and use 5 µL per lane. Additional loading buffer is not required.

Concentration: 0.1 mg/mL

Tracking dye: bromophenol blue and xylene cyanol

Buffer formulation: 10mM Tris-HCl (pH 8.0), 5mM EDTA, 12.5% glycerol, 0.008% bromophenol blue, 0.008% xylene cyanol

Shipping and Storage: SmartCheck™ DNA Ladders are shipped at ambient temperature. On arrival, store at -20°C for optimum stability and long-term storage up to 15 months.

Production: SmartCheck™ ladders are produced from proprietary plasmids, digested to completion. A multistep chromatography method is used to ensure purity and DNA quality.

Quality Control: Each lot of SmartCheck™ DNA Ladders is tested with a spectrophotometer to confirm DNA concentration, as well as agarose electrophoresis to confirm band sharpness.


ItemDescriptionVolume
PR4005-100PR1MA™ SmartCheck™ 50bp DNA Ladder500 µL / 100 Lanes
PR4005-500PR1MA™ SmartCheck™ 50bp DNA Ladder5 x 500 µL / 500 Lanes
PR4010-100PR1MA™ SmartCheck™ 100bp DNA Ladder500 µL / 100 Lanes
PR4010-500PR1MA™ SmartCheck™ 100bp DNA Ladder5 x 500µL / 500 Lanes
PR4100-100PR1MA™ SmartCheck™ 1kb DNA Ladder500 µL / 100 Lanes
PR4100-500PR1MA™ SmartCheck™ 1kb DNA Ladder5 x 500 µL / 500 Lanes
  

PR1MA™ Mammalian Genotyping Kit

  • DNA extraction and amplification in 1 hour
  • Proprietary lysis buffer optimized for ear punches, tail snips and other mammalian tissues
  • Single tube - no organic solvents or clean up procedures
  • Enhanced PR1MA Hot Start Polymerase included

Traditionally, mammalian genotyping protocols have involved Proteinase K digestion, neutralization, organic extraction and lots of hands on time to get PCR-ready DNA.

The PR1MA Mammalian Genotyping Kit is quick and easy to use - add sample to the proprietary lysis buffer and incubate for 5-10 minutes, then add the deactivation buffer and incubate for 10 minutes. The crude lysate can then be amplified using fast PCR PR1MA Hot Start Taq Master Mix with red loading dye (included). The kit contains everything needed - just add sample and primers.

 
 
PR1MA 1 Hour Mammalian Genotyping Kit 

Item

Description

Volume

PR1300-MG-S

PR1MA 1 Hour Mammalian Genotyping Kit

8 Reactions (Sample)

PR1300-MG-80

PR1MA 1 Hour Mammalian Genotyping Kit

80 Reactions

PR1300-MG-400

PR1MA 1 Hour Mammalian Genotyping Kit

400 Reactions

PR1300-MG-800

PR1MA 1 Hour Mammalian Genotyping Kit

800 Reactions


 
 
 PR1MA Genotyping Hot Start Master Mix, 2X Concentration with Red Dye

Item

Description

Volume

PR1301-HSR-400

PR1MA Genotyping Hot Start Master Mix, 2X Conc., Red Dye

400 Reactions

 
 

IBI RT-PCR Certified and Nuclease-Free Water is ideal for all applications in a molecular biology lab including PCR, RT-PCR, restriction enzyme
assays, modifying enzyme assays, transfection, cloning, transformation, and all general molecular biology lab procedures.


IBI PCR Grade water is manufactured under stringent conditions. The purification process includes continuous deionization, reverse osmosis,
UV-treatment, 0.2µm filtration, followed by steam sterilization in an autoclave.

IB42300

PCR Grade Water,nuclease free,1.8 mL vial

IB42301

PCR Grade Water,nuclease free,(20)x 1.8 mL vial

IB42302

PCR Grade Water,nuclease free,(50) x 1.8 mL vial

IB42303

PCR Grade Water,nuclease free,(100) x 1.8 mL vial

Item#:
ASPCRWATER2

Accuris qMAX One-Step RT-qPCR kits 
  • RNA to cDNA to qPCR, in one tube
  • High purity enzyme formulation for enhanced stability and performance
  • Accuris Hot-Start Taq allows for preparation at room-temperature
  • Blue dye facilitates pipetting and visualization in plates
  • Available for green fluorescence or probe detection
  • Multiplex formulation available for multiple target amplification
Accuris qMAX™ One Step Kits allow for highly sensitive real time RT-qPCR assays to be performed directly from RNA templates.  Workflows are simplified with optimized formulations of ready-to-use 2X qPCR master mix and 20X reverse transcriptase.

Optimized buffer includes powerful RNase inhibitors and an extremely thermostable MMLV-derived reverse transcriptase enables robust first strand cDNA synthesis.  Accuris Hot Start Taq uses an antibody mediated hot start mechanism allowing for sample preparation at room temperature.   Only after an initial incubation at 95C will the Taq become active, so non-specific amplification is greatly reduced.  An inert blue dye is included in the Taq master mix to help simplify pipetting and reduce errors.

Three versions of our One Step qPCR kits are available:

qMAX Green One Step kits incorporate our proprietary intercalating dye which exhibits higher fluorescent and lower PCR inhibition than other popular dyes such as SYBR.

qMAX Probe One Step kits are optimized for use with popular TaqMan, Scorpions, and molecular beacon probes.

All Accuris One-Step Kits are compatible with standard and fast cycling protocols and provide increased sensitivity, speed and reproducibility for a broad range of samples and targets.  The polymerase mix is available with different levels of ROX reference dye for compatibility with all qPCR instruments.

Bulk Packaging: 
To accommodate the requirements of high throughput users, Accuris is now offering their full line of qPCR and RT-qPCR reagents in bulk packaging at highly discounted prices. Bottle sizes of 25ml (2500 reactions) and 50ml (5000 reactions) are available for qMAX SYBR Green and qMAX Probe mixes. One Step RT-qPCR kits are also available in bulk format for 2500 and 5000 reactions and are ideal for labs performing pathogen detection.

Please Note:
Low Rox and High Rox formulations are available for compatibility with all brands of qPCR cyclers.  Please specify when ordering.  
All PCR reagents ship in protective coolers with frozen gel packs. 

 

Accuris qMAX Probe Bulk Packaging

Optimized for use with TaqMan™, Scorpions® and molecular beacon probes, qMax Probe qPCR Mix is a ready-to-use formulation for real time quantitative assays.

Same high efficiency for multiplex and singleplex reactions.
  • Includes Accuris Hot Start Taq Polymerase for greater specificity and accuracy.
  • Compatible with popular hydrolysis and beacon probes.
  • Ready to use 2x mastermix.
  • Early Ct values and detection across a broad dynamic range
Optimized for use with TaqMan™, Scorpions® and molecular beacon probes, qMax Probe qPCR Mix is a ready-to-use formulation for real time quantitative assays. qMax Probe utilizes Accuris Hot Start Taq Polymerase or robust PCR with a variety of templates. A specially formulated buffer provides optimal conditions for both superior polymerase function and probe detection, resulting in earlier Ct values and a broad detection range. Complicated, multiplex reactions can be performed without any loss in performance or decrease in detection. The 2X mix requires little, if any optimization and can be used with both fast and standard protocols.
 
qMax Probe qPCR Mix can be used to detect any DNA template, including genomic DNA and cDNA. Available in high, low and no ROX formulations, qMax Probe is compatible with most qPCR instruments.

Bulk Packaging:
To accommodate the requirements of high throughput users, Accuris is now  offering their full line of qPCR and RT-qPCR reagents in bulk packaging at highly discounted prices. Bottle sizes of 25 mL (2500 reactions) and 50 mL (5000 reactions) are available for qMAX SYBR Green and qMAX Probe mixes. One Step RT-qPCR kits are also available in bulk format for 2500 and 5000 reactions and are ideal for labs performing pathogen detection. 

Please Note:
Low Rox and High Rox formulations are available for compatibility with all brands of qPCR cyclers.  Please specify when ordering.  
All PCR reagents ship in protective coolers with frozen gel packs. 

 

Accuris qMAX One-Step RT-qPCR kits

  • RNA to cDNA to qPCR, in one tube
  • High purity enzyme formulation for enhanced stability and performance
  • Accuris Hot-Start Taq allows for preparation at room-temperature
  • Blue dye facilitates pipetting and visualization in plates
  • Available for green fluorescence or probe detection
  • Multiplex formulation available for multiple target amplification
  • Bulk pricing available for high throughput labs and kit manufacturers contact us for details

Accuris qMAX™ One Step Kits allow for highly sensitive real time RT-qPCR assays to be performed directly from RNA templates. Workflows are simplified with optimized formulations of ready-to-use 2X qPCR master mix and 20X reverse transcriptase.

Optimized buffer includes powerful RNase inhibitors, and an extremely thermostable MMLV-derived reverse transcriptase enables robust first strand cDNA synthesis. Accuris Hot Start Taq uses an antibody mediated hot start mechanism allowing for sample preparation at room temperature. Only after an initial incubation at 95C will the Taq become active, so non-specific amplification is greatly reduced. An inert blue dye is included in the Taq master mix to help simplify pipetting and reduce errors.

Three versions of our One Step qPCR kits are available:

qMAX Green One Step kits incorporate our proprietary intercalating dye which exhibits higher fluorescent and lower PCR inhibition than other popular dyes such as SYBR.

qMAX Probe One Step kits are optimized for use with popular TaqMan, Scorpions, and molecular beacon probes.

qMAX Probe One Step Multiplex kits are specifically developed and optimized for efficient probe-based detection of multiple targets in a single reaction well. The Multiplex formulation is comprised of a 20x reverse transcriptase and 2x PCR Mix preparation ideally suited for complex RNA samples including low-copy number viral RNA commonly used in the clinical and research laboratory. qMAX Probe One Step Multiplex kits have been designed to overcome the many challenges of multiplex RT-PCR, by addressing important factors such as the balance between magnesium chloride and deoxynucleotide concentrations, the relative Taq Polymerase and reverse transcriptase concentration, and the ionic conditions of the core reaction buffer.

All Accuris One-Step Kits are compatible with standard and fast cycling protocols and provide increased sensitivity, speed, and reproducibility for a broad range of samples and targets. The polymerase mix is available with different levels of ROX reference dye for compatibility with all qPCR instruments.

Accuris Fast Extraction PCR Kit

 

The Accuris Fast Extraction PCR Kit features an exclusive single-tube solution that extracts genomic DNA in just 8 minutes. This is swiftly followed by PCR amplification utilizing our High Fidelity Hot Start Red Dye Master Mix, designed for superior endpoint PCR performance. High Fidelity Hot Start Red Dye Master Mix is a 2X formula that incorporates a non-reactive red dye, enabling direct gel loading.

 

This kit offers versatility to suit different workflows: the PCR-prepared genomic DNA can be directly used in endpoint PCR or can be applied in real-time PCR using either SYBR Green or TaqMan Probe chemistries.

  • Offers exceptional versatility and can be used to extract and amplify DNA from a wide range of sample types
  • Compatible with animal tissue, plant, saliva, & bacterial samples
  • Extraction & Amplification in less than 60 minutes
  • Includes High Fidelity Hot Start Red Dye Master Mix to prevent the risk of nonspecific amplification & to visualize bands easily
  • Process extracted sample via PCR with included mastermix
  • Alternatively, amplify via qPCR (qPCR mastermix not included)

 

Each 100 reaction kit includes:

1 tube of High Fidelity Hot Start Red Dye Master Mix (1.25 mL)

1 tube of Fast Extraction Lysis Solution (1.25 mL)

 

Applications: Extraction & Amplification of Tissue, Plant, Saliva, & Bacteria   


PR1MA Cod Uracil-DNA Glycosylase

 

PR1MA Cod Uracil-DNA Glycosylase (cUNG) is a recombinant, thermolabile enzyme that removes uracil from DNA. It is ideal for preventing carry over contamination during RNA or DNA amplification reactions that substitute dUTP for dTTP. cUNG is the only commercially available UNG that is completely and irreversibly inactivated by moderate heat treatment, unlike bacterial versions of the enzyme. Cod UNG treatment in combination with targeted pre-amplification using dUTP provides a simple and efficient solution to eliminate carry-over contamination and the generation of false positives and inaccurate quantification.

  • Optimal temperature: 37°C
  • Heat inactivation: 55°C for 5 minutes
  • Enables contamination control in PCR and other amplification methods
  • Does not degrade product after inactivation, enabling downstream use of the amplicon
  • Storage temperature: -20°C

Buffer composition

  • 50% glycerol
  • 50 mM Tris-HCl
  • 50 mM KCl
  • 1 mM DTT
  • 0.1  mM EDTA
  • 0.1% Tween-20
  • pH = 7.5

*These products are intended for research use only, not for therapeutic or diagnostic purposes in humans or animals. The safety and efficacy of these products in diagnostic or other clinical uses has not been established.
 

1mL, 1 vial of 1000ul

Affordable price & shipped at room temperature.

 

A premixed, ready-to-use solution for efficient amplification of DNA templates by PCR.

  • 2X Eco-Taq MasterMix contains Taq DNA Polymerase, dNTPs, Mg2+ and Reaction Buffer at optimal concentrations for efficient amplification of DNA templates by PCR, as well as an inert loading dye.
  • This pre-mixed formulation saves time and reduces contamination due to the fewer pipetting steps required for PCR set up. To prepare the final PCR, only primers and template DNA need to be added.
  • The mix retains all features of Taq DNA Polymerase and can amplify DNA targets up to 5 kb (simple template).
  • The elongation velocity is 0.9~1.2kb/min (70~75°C).
  • It has 5' to 3' polymerase activity but lacks 3' to 5' exonuclease activity which results in a 3'-dA overhang PCR product.

 

Features:

  • Convenient: Just add primers and template DNA
  • High yields of PCR products with minimal optimization.
  • High efficiency: saves your time by simplifying the process
  • Reproducible: lower contamination risk and pipetting error.

    Applications:

  • High-throughput PCR.
  • Routine PCR with high reproducibility
  • Generation of PCR products for TA cloning

    Contents:
    2X Taq Mix 1ml

    Store at -20°C
    For research use only

Item#:
ECO-TAQ1
Your Price:
35.00
Each


PR1MA Tn5 2.0 Transposase

 

PR1MA Tn5 2.0 Transposase (Tnp) is a hyperactive retroviral integrase engineered for improved activity, speed, and robustness that is used to construct random next-generation sequencing libraries and to study chromatin structure using targeted ATAC-seq. PR1MA Tn5 2.0 can be used to randomly fragment any target and insert unique oligonucleotide adapters in a single reaction which reduces the time and sample requirements relative to traditional next-gen sequencing library construction. 

  • Optimal temperature: 55°C
  • Inactivation: 40X Stop solution included (2% SDS)
  • Storage temperature: -20°C
  • 10X Tn5 reaction buffer included

Buffer composition

  • 100 mM Tris-HCl
  • 100 mM MgCl2
  • pH = 7.5

*These products are intended for research use only, not for diagnostic use. The safety and efficacy of these products in diagnostic or other clinical uses has not been established.   
 


PR1MA Taq DNA Polymerase

 

Saves time and cost by enabling direct PCR amplification of unpurified templates. PR1MA Taq DNA polymerase is a recombinant, truncated (lacks 5’ to 3’ exonuclease activity), highly thermostable DNA polymerase from the thermophilic bacterium Thermus aquaticus. The enzyme is thermostable up to 98°C for polymerase chain reaction assays. It is supplied with 4 M betaine to improve amplification of GC-rich DNA and 30% sucrose to improve amplification from inhibitor-rich substrates such as blood.

  • Lacks exonuclease activity
  • Thermotolerant up to 98°C
  • Resistant to inhibitors, e.g., whole blood
  • Ideal for GC-rich templates
  • Storage temperature: -20°C
  • 10X PR1MA buffer, 4 M Betaine, and 30% Sucrose included

Buffer composition

  • 50% glycerol
  • 50 mM Tris-HCl
  • 50 mM KCl
  • 1 mM DTT
  • 0.1  mM EDTA
  • 0.5% Tween-20
  • 0.5% NP-40 substitute
  • pH = 7.5 

Important note: Please be sure to use the buffer provided with this product to ensure optimal results.

 

*These products are intended for research use only, not for therapeutic or diagnostic purposes in humans or animals. The safety and efficacy of these products in diagnostic or other clinical uses has not been established.
 

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