Adhesive Sealing Film, Aluminum, 1.4 mil

PR1MA™ Taq DNA Polymerase 1X Master Mix

PR1MA™ DNA polymerase 1X Master Mix saves time and cost by enabling direct PCR amplification of unpurified templates. It contains a recombinant, truncated (lacks 5’ to 3’ exonuclease activity), highly thermostable DNA polymerase from the thermophilic bacterium Thermus aquaticus. The enzyme is thermostable up to 98°C for polymerase chain reaction assays and is provided as a complete reaction master mix consisting of reaction buffer, dNTPs, MgCl2, and loading dye and only requires the addition of primers and DNA template. Once PCR is complete, the reaction products can be loaded directly into an agarose gel for analysis.

• Lacks exonuclease activity
• Thermotolerant up to 98°C
• Inhibitor Resistant
• Ideal for colony PCR, genotyping, and GC-rich templates
• Storage temperature: -20°C 

Important note: Please be sure to use the buffer provided with this product to ensure optimal results.

*These products are intended for research use only, not for therapeutic or diagnostic purposes in humans or animals. The safety and efficacy of these products in diagnostic or other clinical uses has not been established.

• Quickly Spin down droplets and condensation
• Use before and after thermal cycling to increase PCR yield
• Accepts skirted, Non-Skirt and all standard PCR plates
• Less than 1/4 the size of most plate centrifuges
• 1 year warranty

Mini Plate Spinner Adapter Supports:

• 8 x 0.2ml PCR Strips
• 12 x 0.2ml PCR Strips
• 0.2ml PCR Tubes
• 48 well PCR Plates

Please know that the manufacturer has suspended manufacturing this product indefinitely. 
We suggest purchasing PR1MA PCR Plastics.
Contact your rep or email tech@midsci.com for more information.

Platemax CyclerSeal Sealing Film

• Provides exceptional seal to safeguard samples during transport or storage
• Eliminates well to well contamination and cross-over in PCR applications when used with Axygen's compression mat
• Polypropylene film is wide temperature range compatible, -40° to 104°C
• PCR-TS is designed for ELISA/EIA applications
• PCR-TS-900 is twice as thick (as PCR-TS) and is suitable for water-bath and other difficult PCR applications

• Provides exceptional seal under the widest temperature conditions and reagents
• Wide temperature range compatible, -80° to 104°C
• Ideal for light sensitive samples and PCR or storage
• Uniform adhesive provides optimum well sealing
• PCR-AS-200 is pierceable
• 100 films/pack

LabNet LabValues Promotions 2024!

For a limited time enjoy discounted pricing on the AccuSeal Semi-Automated Plate Sealer (PS1000)
 

 See flyer for more details.

 

Promo is valid 07/1/24 – 12/31/24.

Not to be combined with any offers or discounts. No institutional pricing. All terms apply.   

AccuSeal Semi-Automated Plate Sealer

AccuSeal Semi Automated Plate Sealers from MIDSCI are engineered and constructed by Labnet International, Inc to maximize PCR, assay, and storage applications with a user-friendly panel and automated sealing options.

Great emphasis has been placed on incorporating a wide range of standard heights to deep well microplates with appropriate adaptors to efficiently fit the plates for consistent temperature regulation. The AccuSeal sealer is optimized for simple digital control and operation, user-friendly versatility and convenience as well as safety.
 

Controls and Operation:

• Intuitive layout permits optimal sealing time, temperature and settings
• Includes a "No Heat" setting for pressure sealing applications

Versatility and Convenience:

• Adaptors specific for a PCR plate or an assay plate accommodate a variety of fits
• Automatically adjusts heat for different height plates
• Provides uniform pressure for a quality seal with a special non-stick coating to prevent messy cleanup.

Safety:

• Motorized drawer features safety switches and prevents injury to the user.

Specifications
 

Dimensions	6.75x12.75x14.25 inches
Weight	24 Lbs
Sealing Temperature	OFF, 100 to 190 °C
Temperature Accuracy	+/- 1.00 °C
Temperature Uniformity	+/- 1.00 °C
Sealing Time	0.5 to 10 sec
Compatible Plate Materials	PP (Polypropylene) PS (Polystyrene) PE (Polyethylene)
Compatible Plate Types	Standard Assay Plates Deep-well Storage Plates PCR Plates: skirted, semi-skirted, non-skirted
Maximum Plate Height	45mm
Compatible Sealing Film Types	Foil-polypropylene laminate Clear polyester-polypropylene laminate Clear polymer Thin clear polymer Foil-laminate Foil

Adapters 
 

PS1000-PCR	AccuSeal PCR plate adapter for sealing PCR and 384 well plates
PS1000-ADAPT	AccuSeal Assay plate adapter for sealing assay plates

Sealing Film

1x8 FilmStrips™ for Sealing Only One 8-well Row of a 96-well Plate

SmartSeal™ Semi-Automated Microplate Sealer

• Reduces formation of non-specific products
• Improves results from multiplex reactions
• Essential for low copy number
• Buffer II: potassium/ammonium buffer for multiplex reactions
• Activated at elevated temperatures
• Gives higher specificity and greater yields than standard Taq
• Chemical moiety is attached to the enzyme at the active site and renders it inactive at room temperature; is cleaved during a 15 min. heat activation step
• Prevents mispriming during setup and the first ramp of thermal cycling

This brand is being discontinued, please contact us for other options.

Looking for other alternatives?

Visit our PR1MA Polymerase page for more options!

PR DNA Polymerase, High Fidelity, 2.5 U/µL

• Provides higher fidelity than standard Taq DNA Polymerase
• Produces blunt-ended fragments
• Processes <3 kb with extremely high fidelity

Store at -20°C. For in-vitro laboratory use only

Bullseye PREMIUM PR DNA Polymerase is a thermostable enzyme with proofreading ability, which can be used in primer extension reactions and other molecular biology applications. PR Polymerase exhibits both 5'-3' DNA polymerase activity and 3'-5' proofreading exonuclease activity. It is recommended for applications, which require extremely high fidelity or blunt ending.

Optimal reaction conditions are achieved by using the 10x Ammonium buffer containing MgCl2 provided with the enzyme. 25 mM MgCl2 is also included separately, in case a higher MgCl2 concentration is required for a specific reaction.

Unit Definition
One unit is defined as the amount that incorporates 10 nmoles of dNTPs into acid-precipitable form in 30 minutes at 72°C under standard assay conditions.

10X Ammonium Reaction Buffer
Tris-HCl pH 8.5, (NH4)2SO4, 1% Tween20,
15mM MgCl2

PR Storage Buffer
50 mM Tris-HCl (pH 8.0), 50 mM NaCl, 0.1 mM EDTA, 1 mM DTT, 50% Glycerol, 0.1% NP40, 0.1% Tween-20.

Quality Control
Endonuclease, exonuclease and priming activities are not detected after 3 hours incubation of 1 mg of pUC19 plasmid DNA and 0.5 µg EcoR I digested lambda phage DNA at 72°C in the presence of 40 units of PR DNA Polymerase.

Suggested Protocol using PR DNA Polymerase
This protocol serves as a guideline. Optimal reaction conditions must be individually determined.

1. Thaw 10X Ammonium Buffer, dNTP mix, and primer solutions. It is important to mix the solutions completely before use to avoid localized concentrations of salts.

2. Prepare a master mix according to Table 1. The master mix typically contains all the components needed for extension except the template DNA.
The optimal MgCl2 concentration should be determined empirically but in most cases a concentration of 1.5 mM, as provided in the 1X Ammonium Buffer, will produce satisfactory results. Table 2 provides the volume of 25 mM MgCl2 to add to the master mix if a higher MgCl2 concentration is required.

Table 1. Reaction components (master mix and template DNA)

Table 2. MgCl2 concentration in a 50µl reaction

3. Mix the master mix thoroughly and dispense appropriate volumes into reaction tubes. Mix gently, e.g., by pipetting the master mix up and down a few times.

4. Add template DNA (0.1-0.5 mg/reaction) to the individual tubes containing the master mix.

5. Program the thermal cycler according to the manufacturer's instructions. PR is a proofreading enzyme and requires an extension time of 1-2 min/kb. For maximum yield and specificity, temperatures and cycling times should be optimized for each new template target or primer pair.

HS Taq Polymerase

5 units/µl

Store at -20°C. For in-vitro laboratory use only

General Description

Bullseye HS Taq DNA Polymerase is a modified form of Bullseye Taq DNA Polymerase, which is activated by heat treatment. A chemical moiety is attached to the enzyme at the active site, which renders the enzyme inactive at room temperature. Thus, during setup and the first ramp of thermal cycling, the enzyme is not active and misprimed primers are not extended. The result is higher specificity and greater yields when compared to standard DNA polymerases.

Once the reaction reaches optimal activating temperature, the chemical moiety is cleaved during a 15 minute heat activation step, releasing the active HS Taq DNA Polymerase into the reaction.

10X HS Buffer I

Tris-HCl, pH 8.5 (NH4)2S04, 15 mM MgCl2, 1% Tween 20.

10X HS Buffer II

An optimized buffer with a balanced ammonium/potassium concentration. May improve results with more complicated PCR reactions such as multiplex PCR.

Tris-HCl pH 8.7, Balanced KCl/(NH4)2S04, 15 mM MgCl2, 1% Tween 20.

HS Taq Storage Buffer

Enzyme is supplied in 20 mM Tris-HCl pH 8.3, 100 mM KCl, 0.1 mM EDTA, 1 mM DTT, 0.5% Tween 20®, 0.5% NP40, 50% glycerol.

Unit Definition

One unit is defined as the amount that incorporates 10 nmoles of dNTPs into acid-precipitable form in 30 minutes at 72°C under standard assay conditions.

Quality Control

Endonuclease, exonuclease and priming activities are not detected after 3 hours incubation of 1 mg of pUC19 plasmid DNA and 0.5 µg EcoR I digested lambda phage DNA at 72°C in the presence of 40 units of TEMPase DNA Polymerase.

Suggested Protocol using HS Taq DNA Polymerase

This protocol serves as a guideline. Optimal reaction conditions must be individually determined.

• 15 mM MgCl2 is present in the 10X HS Buffer I and II. However, in some applications, more than 1.5mM MgCl2 is needed. For this reason, 25mM MgCl2 is included with the kit. Table 2 provides the volume of 25mM MgCl2 to add to the master mix if a higher MgCl2 concentration is required.

  1. Thaw 10X HS Buffer I or/and 10X HS Buffer II, dNTP mix, primer solutions. It is important to mix the solutions completely before use to avoid localized concentrations of salts.

  2. Prepare a master mix according to Table 1. The master mix typically contains all the components needed for extension except the template DNA.

Table 1. Reaction components (master mix & template DNA)

Table 2. MgCl2 concentration in a 50 µL reaction