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PCR/qPCR

MIDSCI can supply almost every needed product for PCR/qPCR: tubes, plates, strips, caps, taq, Mastermixes, dNPTs, dye and probe for qPCR, sealing film and much more.  If you are looking for any of these things, we can match almost all needs to all cyclers.  If you don’t find it easily contact us at tech@midsci.com and we will find it for you. 

96 or 384 we have it.  Non-Skirted, semi-skirted or full skirted, we have it.  0.1, 0.2, or 0.5 mL we have it.  A1, A12, H12, A24, P24 cuts, we have them.  Sybr© Green or probe alternative, we have it.  

Pro Tip: Colored plastics do not have any impact on DNA amplification.  But when setting up qPCR it is often recommended to use white plastics.  This will limit or prevent fluorescence refraction.  Light refraction can minimize sensitivity and consistency. 

Pro Tip 2: If you are seeing inconsistencies in PCR/qPCR try PR1MA Pipette Tips.  This will help optimize and reduce variance in the reactions.  Simple test, see the residual liquid left in tips?  This means some of the taq, dNTPs, sample, etc is left in the tip.  PR1MA tips help reduce that significantly and improve your consistency. 

Pro Tip 3: Seeing evaporation in the outer wells of a PCR/qPCR plate?  Sometimes it’s the plate, sometimes it the seal, and sometimes it’s the cycler.  Call us we have solutions.

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While Supplies Last!

This brand is being discontinued and will only be available while supplies last.

 

Need great (q)PCR Regents at a great price?

Try our PR1MA™ Taq and save!

 
  • Excellent all-purpose amplification enzyme
  • Thermostable recombinant DNA polymerase from Thermus aquaticus
  • Exhibits very high activity in primer extension
  • Has both a 5' to 3' DNA polymerase and a 5' to 3' exonuclease activity
  • Does not have 3' to 5' exonuclease activity-no proofreading ability
  • Leaves an A-overhang, which makes the enzyme ideal for TA cloning
  • Includes MgCl² in buffer
Item#:
ASPCRREAG1
Discontinued, Contact us for more options!


This brand is being discontinued, please contact us for other options.


Looking for other alternatives?

Visit our PR1MA Polymerase page for more options!



Store at -20°C. For in-vitro laboratory use only

General Description

Bullseye Taq DNA Polymerase is a thermostable recombinant DNA polymerase, which exhibits very high activity in primer extension and other molecular biology applications. The enzyme is isolated from Thermus aquaticus and has a molecular weight of approximately 94 kDa.

Bullseye Taq DNA Polymerase has both a 5'®3' DNA polymerase and a 5'®3' exonuclease activity. The enzyme lacks a 3'®5' exonuclease activity (no proofreading ability). Taq DNA Polymerase leaves an A¢ overhang, which makes the enzyme ideal for TA cloning.

The 10X Reaction Buffer provided does not contain Mg+2. 25 mM MgCl2 is supplied separately.

10X Mg++ Free Standard Buffer

100 mM Tris-HCl pH 8.3, 500 mM KCl.

Unit Definition

One unit is defined as the amount that incorporates 10 nmoles of dNTPs into acid-precipitable form in 30 minutes at 72°C under standard assay conditions.

Storage and Dilution Buffer

Enzyme is supplied in 20 mM Tris-HCl pH 8.3, 100 mM KCl, 0.1 mM EDTA, 1 mM DTT, 0.5% Tween 20, 0.5%NP40, 50% glycerol.

Quality Control

Each lot of Taq DNA Polymerase is tested for contaminating activities, with no trace of endonuclease activity, nicking activity, exonuclease activity or priming activity.

Suggested Protocol using Taq DNA Polymerase

This protocol serves as a guideline only. Optimal reaction conditions may vary and must be individually determined.

n In some applications, MgCl2 is needed for the best results. For this reason, 25 mM MgCl2 is included with the kit.

1. Thaw 10X Mg2+ Free Standard Buffer, dNTP mix, primer solutions. It is important to mix the solutions completely before use to avoid localized concentrations of salts.

2. Prepare a master mix according to Table 1. The master mix typically contains all the components needed for extension except the template DNA.

Table 1. Reaction components (master mix & template DNA)

 

Component

Vol./reaction

Final Conc.

10X Mg2+ Free Buffer

5 µl

1X

MgCl2, 25 mM

1- 9 µl

0.5  4.5 mM

dNTP mix

(12.5 mM of each)

0.8 µl

0.2 mM of

each dNTP

Primer A

Variable

0.11.0 µM

Primer B

Variable

0.11.0 µM

Taq DNA Polymerase

Variable

1-5 units

Template DNA

Variable

Variable

Distilled Water

Variable

- - - -

TOTAL volume

50 µl

- - - -

Table 2. MgCl2 concentration in a 50 mL reaction

 

Final MgCl2 conc. in reaction (mM)

0.5

1.0

1.5

2.0

2.5

3.0

3.5

4.0

4.5

Volume of 25 mM MgCl2

per rxn (µl):

1

2

3

4

5

6

7

8

9

Table 2 provides the volume of 25 mM MgCl2 to add to the master mix if a certain MgCl2 concentration is required.

3. Mix the master mix thoroughly and dispense appropriate volumes into reaction tubes. Mix gently, e.g., by pipetting the master mix up and down a few times.

4. Add template DNA to the individual tubes containing the master mix.

5. Program the thermal cycler according to the manufacturers instructions. For maximum yield and specificity, temperatures and cycling times should be optimized for each new template target or primer pair.

6. Place the tubes in the thermal cycler and start the reaction.

Item#:
ASPCRREAG2
While Supplies Last!

This brand is being discontinued and will only be available while supplies last.


Looking for other alternatives?

Visit our PR1MA Polymerase page for more options!


 

2x Master Mix Kit (1.5 mM MgCl2) 

Bullseye Taq DNA Polymerase Mix is a ready-to-use 2x reaction mix. Simply add primers, template, and water to successfully carry out primer extensions and other molecular biology applications.

Bullseye Taq polymerase, the NH4+ buffer system, dNTPs, and magnesium chloride are conveniently present in the Taq DNA Polymerase Mix. (Inert Red Dye is present in BE180303 only)

Bullseye Taq DNA Polymerase Mix offers several advantages. Set up time is significantly reduced. The chance of contaminating component stocks is eliminated. Reduction of reagent handling steps leads to better reproducibility. Standard tests can be set up with the confidence that results will be consistent every time.

Composition of 2x Taq Master Mix

150 mM Tris-HCl pH 8.5, 40 mM (NH4)2S04, 3 mM MgCl2, 0.2% Tween 20Ò

4 mM dNTPs

2 units/µL AS ONE Taq polymerase

Inert Red Dye & Stabilizer (BE180303 only)

Item#:
ASPCRREAG3
Discontinued, Contact us for more options!

This brand is being discontinued, please contact us for other options.
 

Looking for other alternatives?

Visit our PR1MA Polymerase page for more options! 





R-Taq DNA Polymerase 5 units/µL
 
 

Cat. No.Units10X Ammonium Buffer
(MgCl2 15mM)		MgCl2
25 mM
BE2003035001.5 mL1.5 mL
BE2003041,0002x 1.5 mL2x 1.5 mL
BE2003062,5004x 1.5 mL4x 1.5 mL


Store at -20°C. For in-vitro laboratory use only

 

General Description

Bullseye R-Taq DNA Polymerase is a thermostable recombinant DNA polymerase, which exhibits very high activity in primer extension and other molecular biology applications. R-Taq contains a red dye which provides easy and quick identification of reactions to which enzyme was added and allows confirmation of complete mixing. The inert dye has no effect on downstream processes. R-Taq is added directly to the reaction mix and is used in the same manner as standard Taq DNA Polymerase.

Bullseye R-Taq DNA Polymerase has both a 5'®3' DNA polymerase and a 5'®3' exonuclease activity. The enzyme lacks a 3'®5' exonuclease activity. R-Taq DNA Polymerase leaves an A-overhang, which makes the enzyme ideal for TA cloning.

  • High performance thermostable DNA polymerase
  • Red dye identifies tubes which contain enzyme and confirms complete mixing of reagents
  • Leaves an A-overhang

Unit Definition

One unit is defined as the amount that incorporates 10 nmoles of dNTPs into acid-precipitable form in 30 minutes at 72°C under standard assay conditions.

Storage Buffer

Enzyme is supplied in 20 mM Tris-HCl pH 8.3, 100 mM KCl, 0.1 mM EDTA, 1 mM DTT, inert dye, 0.5 % Tweenà 20, 0.5% NP40, 50% glycerol.

Component

Vol./reaction

Final Conc.

10X Ammonium Buffer

5 µL

1X

dNTP mix (12.5 mM each)

0.8 µL

0.2 mM each dNTP

Primer A

Variable

0.1-0.5 µM

Primer B

Variable

0.1-0.5 µM

R-Taq DNA Pol

1 mL

5 units/reaction

Distilled Water

Variable

- - - -

Template DNA

Variable

0.1-0.5 µg/reaction

TOTAL volume

50 µL

- - - -

Table 2. MgCl2 concentration

3. Mix the master mix thoroughly and dispense appropriate volumes into reaction tubes. Mix gently (e.g., by pipetting) the master mix up and down a few times.

4. Add template DNA (0.1-0.5 mg/reaction) to the individual tubes containing the master mix.

5. Program the thermal cycler according to the manufacturer's instructions.

For maximum yield and specificity, temperatures and cycling times should be optimized for each new template target or primer pair.

6. Place the tubes in the thermal cycler and start the reaction.

7. After primer extension, load 5-10 mL of a 50 mL reaction directly on an agarose gel for analysis.


Tween 20 is a registered trademark of ICI Americas, Inc.


Final MgCl2 conc.

in reaction (mM)

1.5

2.0

2.5

3.0

3.5

4.0

4.5

Additional volume

of 25 mM MgCl2

per reaction (µL):

0

1

2

3

4

5

6

10X Ammonium Reaction Buffer

Tris-HCl pH 8.5, (NH4)2S04, 15 mM MgCl2, 1% Tween 20

Quality Control

Endonuclease, exonuclease and priming activities are not detected after 3 hours incubation of 1 mg of pUC19 plasmid DNA and 0.5 µg EcoR I digested lambda phage DNA at 72°C in the presence of 40 units of R-Taq DNA Polymerase.

Suggested Protocol using R-Taq Polymerase

This protocol serves as a guideline. Optimal reaction conditions such as incubation times, temperatures, and amount of template DNA may vary and must be individually determined.

1. Thaw 10X Ammonium Buffer, dNTP mix, and primer solutions. It is important to mix the solutions completely before use to avoid localized concentrations of salts.

2. Prepare a master mix according to Table 1. The master mix typically contains all the components needed for extension except the template DNA.

The optimal MgCl2 concentration should be determined empirically but, in most cases a concentration of 1.5 mM, as provided in the 1X Ammonium Buffer, produces satisfactory results. Table 2 provides the volume of 25mM MgCl2 to add to the master mix if a higher MgCl2 concentration is required.

Table 1. Reaction components (master mix & template DNA)

Item#:
ASPCRREAG4
This item has been discontinued and replaced by item 

PCR SILICONE SEALING MAT - 96

  • Made of research-grade silicone
  • Scored wells for sample access
  • Perforations re-seal after access
  • Resists coring and tearing
  • Eliminates cross-talk between wells
  • Also works as a compression mat
  • Suitable for standard PCR thermocycling
  • Mats can be reused
  • For use with 96-well PCR plates
  • Printed alpha numeric grid for easy reference
  • Certified to be free of RNase, DNase, DNA, PCR inhibitors, and Pyrogen contamination.
  • 10 Mats/Bag, 5 Bags/Case

Works as a plate seal (to eliminate cross contamination) and compression mat all-in-one!

Our compression molded 96-round plug, silicone sealing mats for PCR plates minimize sample evaporation in a wide range of temperatures. These mats are chemically resistant and reusable; containing no adhesives or extractables to contaminate samples.  Made of research-grade silicone, they can be autoclaved, and following a 10% bleach wash and ethanol rinse protocol may be reused.

Wells are scored (cut 80% of the way through, but not all of the way) to allow for sample access via pipettor, syringe, or probe.  These scored perforations will seal themselves, after penetration, ensuring an airtight closure to the well.
Item#:
MID-PCMATR-9-PRT
Your Price:
208.50
Each
This item is discontinued
Strips, 1000/cs for

These Rotocycler strips are perfectly designed to perform on Rotor-Gene instruments. Tube strips are packaged separately from cap strips. The frosted extensions on caps not only make them more efficient and secure during handling but also offer a convenient area for labeling. For individual use, tube and cap strips can easily be separated and used as individual units.

Item#:
T319-4N
Your Price:
526.77
Each

Double sided water cooled vertical system, 10cm x 10cm


Reflection double sided water cooled vertical system, 10cm x 10cm with or without casting base, two pack each of plain and notched glass, one acrylic blocker plate, (2) 10-well 0.8mm thickness combs, (4) 0.8 mm spacers. Four year warranty.

System Description
 
Run either one or two gels simultaneously on these versatile separation systems. The 10x10cm size of the system also accomodates most pre-cast gels.

Complete double sided vertical system for running one or two gels, in a 10 x 10 cm format. Unique casting bases allows both casting and running of gels without unclamping from the upper buffer chamber, providing for safer and more convenient gel handling. In addition the 1010 device will accommodate most precast gel cassettes on the market.

Water cooling feature is included for protocols that require it. Connections are easily made by attaching standard tubing over the 3/8" barbed in/out ports provided on the device. Water may be provided by tap or other temperature controlled water source. Optional alumina backer plates may be used to facilitate cooling efficiency and uniformity, and are available in the accessories list below.

System Features
 
The Galileo Reflection™ 85-1010 complete system includes these features:

  • Heavy duty upper buffer chamber with cooling port connections.
  • Lower buffer chamber.
  • Interlock safety lid with attached leads.
  • Casting base.
  • Two-pack each of plain & notched glass plates.
  • One acrylic blocker plate (used when running only one gel).
  • Ttwo 10-well, 0.8mm thick combs
  • Four 0.8mm thick side spacers.
  • System Accessories
  • Choose amoung these accessories to customize each system for your specific needs.
  • Alumina cooling plate.
  • For use with cooling systems.
  • This plate can be sandwiched between the gel and the inner buffer chamber to improve overall temperature uniformity.
  • Casting base.
  • For quick, efficient and leak-proof casting.
  • Multiple gradient caster.
Item#:
ASDOUBSIDEWAT10C

Extra Wide Large Format Gel System, 23.5cmW x 40cm

 
Extra wide large format gel system, 23.5cmW x 40cmL gel size with buffer exchange ports, UVT gel tray with fluorescent ruler and 12 tray slots, (2) 25 teeth combs, 1.5 mm thickness and (2) 50 teeth combs, 1.5 mm thickness.
System Description

For long run lengths, maximum resolution, and really large sample capacity, look no further than the Galileo 2340 device. This unit also features the Galileo ExpressCast system for quick tape-less gel pouring.

The 2340 is our maximum capacity system. With a maximum run length of 25cm, the unit can provide very high resolution of complex analytes. Or, fitted with maximum capacity combs in all of the tray's 12 comb slots, the unit can handle up to 600 samples. The 2340 features the Galileo ExpressCast gel casting system for quick, tape-free gel casting and includes a built-in bubble level and leveling screws for convenient level adjustment. You may choose from a wide variety of comb options to customize your order.
System Features

The Galileo 2340 complete system includes these features:

  •     Heavy duty lower buffer chamber
  •     Interlock safety lid with attached leads
  •     End gated 23cm wide x 40cm long ExpressCast™ UV-transparent gel tray with built-in fluorescent ruler and 12 comb slots.
  •     Two 1.5mm x 25 tooth combs
  •     Two 1.5mm x 50 tooth combs
Item#:
ASEXTRAWIDELGFOR23

MaGELin Mini Protein System

  • For 10 cm x 10 cm cast-your-own polyacrylamide gels
  • Comes with dual casting system
  • 3 sets of glass plates and 2 sets of 0.8 mm thickness spacers.
  • Buffer tank, dual gel capture, vented lid and power cords included

The IB94000 MaGELin Mini protein system comes complete with everything you need to pour 10 cm x 10 cm cast-your-own gels. This highly versatile system will also accommodate the IB95000 Western Transfer module so when you’re not running gels the unit can be used as a dedicated Western transfer system.
 

The IB94000 comes complete with:

  • Dual gel capture device for running two gels simultaneously.
  • Dual gel casting system that allows casting outside of the running unit.
  • Three sets of glass plates for pouring and running gels
  • Two sets of special 0.8 mm side spacers to seal the gel sandwich during pouring
  • Buffer tank
  • Vented lid
  • Power cords.
  • Two 0.8 mm x 12-tooth combs.

The IB94000 utilizes a unique spacer and casting system to make it easy and fast to cast your own gels. The special side spacers are made specifically to accommodate the thickness of each glass slide that comes with the unit. These side spacers will produce a 0.8 mm thick gel. Once the glass plates have been assembled using the side spacers, they can now be inserted into the gel casting unit. This unit accommodates two sets of glass plates simultaneously. The bottom of the gel casting unit contains a very soft gel-like gasket that will seal the bottom of the gel sandwich. Once the gel sandwich is placed inside the casting unit, place the lid of the casting unit on top and press gently to fasten using the included thumb screws. Once fastened the lid will provide the correct amount of down pressure on the gel sandwich to seal the bottom and hold them in place. The polyacrylamide solution can now be introduced through the opening in the casting fixture lid. 

Item#:
ASMAGELINMINI
White Light, 21x26cm, Single

Uniform white light diffusion for viewing negative and film positives. X-ray film, autoradiographs, microtiter plates, and stained gels can be viewed on the white light transmitting glass surface. This surface, permanently double-safety sealed into the unit for durability, provides a scratch and chemical resistant work area which can be cleaned easily. Durable transilluminator housing is scratch resistant, epoxy powder paint finish for easy cleaning. 2 year warranty

ModelOrder NoDescriptionFilter SizeDimensions
TW-26 95-0208-01White Light Transilluminator, 115V 21 x 26cm9.5"D x 13.25"W x 4.25"H
TW-4395-0214-01White Light Transilluminator, 115V 36 x 43cm19.13"D x 16"W x 4.25"H

Safety Certifications: ETL, ETL/c on all 115V models.
Item#:
95-0208-01
Your Price:
1321.11
Each

Extra Wide Format Gel System, 23.5 cm x 25 cm

 ExpressCast extra wide format gel gystem for 23.5cmW x 25cmL gel size. Includes UVT gel tray with fluorescent rulers and 14 comb slots, (2) 25 tooth combs, 1.5 mm thickness, and (2) 50 tooth combs, 1.5 mm thickness and gasketed end-gates.
System Description

Need long run lengths for maximum resolution, and large sample capacity too? Look at the features of the Galileo 2325 unit and we think you will find just what you need.

The 2325 is a full sized large capacity system. With a maximum run length of 25cm, the unit can provide high resolution of complex analytes. Or, fitted for maximum capacity, the unit can handle up to 500 samples. The 2325 features the Galileo ExpressCast gel casting system for quick, tape-free gel casting and includes a built-in bubble level and leveling screws for convenient level adjustment. You may choose from a wide variety of comb options to customize your order.
System Features

The Galileo 2325 complete system includes these features:

  •     Heavy duty lower buffer chamber
  •     Interlock safety lid with attached leads
  •     End gated 23cm wide x 25cm long ExpressCast™ UV-transparent gel tray with built-in fluorescent ruler and 14 comb slots.
  •     Two 1.5mm x 25 tooth combs
  •     Two 1.5mm x 50 tooth combs
Item#:
ASEXTRAWIDEFOR23

Double sided water cooled vertical system, 20cmW x 10cm


The Galileo Bioscience Reflection 2010 is a wide, high capacity gel electrophoresis separation system that allows users to run up to two protein gels simultaneously on a 20 cm wide x 10 cm tall format.  The large gel format makes this unit ideal for users who have a large number of protein samples to run simultaneously.  Galileo's unique casting base allows users to both cast and run gels without ever having to unclamp the upper buffer chamber.  This makes handling gels safer and more convenient, especially for those who may be prone to ripping their gels between the two activities. 

The 2010 vertical gel box also includes a water cooling feature for electrophoresis protocols that require lower temperature.  This water can be provided by the tap or other temperature controlled source and is easily connected by attaching standard tubing over the 3/8" barbed in/out ports on the device.  Users may also facilitate cooling efficiency and uniformity using Galileo's optional alumina backer plates (see accessories below).

System Features

Each Reflection 85-2010-V vertical system includes the following features:

  • Heavy duty upper buffer chamber with cooling port connections
  • Lower buffer chamber
  • Interlock safety lid with attached leads
  • Casting base
  • Four-pack each of plain and notched glass plates
  • One acrylic blocker plate (used when only one gel is being run)
  • Eight 0.8mm thick side spacers
  • Four combs of your choice
  • System Accessories

Alumina cooling plate for use with cooling systems. This plate can be sandwiched between the gel and the inner buffer chamber to improve overall temperature uniformity.
 
Casting base for quick, efficient and leak-proof gel casting.
Item#:
ASDOUBLESIDEWATVER20
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