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Prime Pro 48 Real Time PCR system

Item #:ASPCRTC7 

Key benefits
Speed

  • 40 cycles in 40 minutes before fast optimization.
  • 40 cycles in 15 minutes when optimized.
  • Genotype in 4 minutes with over 99% accuracy.
  • Set up a 4plex reaction in only 4 mouse clicks.

    Confidence
  • MIQE compliant - always consistent, always compliant.
  • Instant settle time to achieve world leading uniformity of ±0.1°C across the block - any well, any instrument - consistent results in any system.
  • 400 specific qPCR reagent kits available.


    Performance
  • Consistent results provide trust and confidence. Well-to-well, run-to-run , machine-to-machine.
  • Compile data from multiple experiments. Pool plates and results across labs and instruments.
  • Record every well, with every filter, in every cycle. Never miss a data point.
  • Adaptive LED Control means you never have uncertainty of saturated unknowns, no bleaching though light to neighboring wells or maximizing the wide dynamic range. Trust results are correct. 
  • All other block-based qPCR machines have lower uniformity. If you perform Next Generation Sequencing, any other instrument than Prime Pro 48 is risking your NGS, with significant cost implications.

    Value
  • No other qPCR system offers so much performance for such a low price.
  • Available as a complete system - Techne instrument, plates, seals and reagents.
  • Alternatively, is chemically open platform - use your preferred supplier reagents.
  • Lowest price per run - use duplicates and low sample volumes. Don't waste money with triplicates and large sample volumes.
  • Everything as standard - no add-ons, HRM as standard, unlimited software license. 
  • Highly accurate and uniform qPCR prevents means successful NGS runs and significant moneysaving.

    Sensitivity
  • ±0.1°C uniformity across the whole block instantly after every temperature change means that any well, in any instrument, provides same answer
  • HRM - Identify Class IV SNP >99.9% of the time
  • Unsurpassed block uniformity with patented block technology enables the researcher to use duplicates on Prime Pro 48, whereas triplicates are required on less uniform competitor machines.

     

    The Prime Pro 48 real time PCR system is a new, high specification, economically priced real time thermal cycler from Techne. Made in the UK, the Prime Pro 48 features the highest thermal block uniformity and fastest run time of any block-based qPCR system on the market.

    High thermal uniformity and validated sample volumes down to 5ul ensures Prime Pro 48 allows researchers to bring run times for an optimized 40 cycle reaction down to only 15 minutes. PCR efficiency relies on precise temperature control during the denaturation and annealing steps. For the highest accuracy, temperature must remain uniform across the plate, ensuring that all samples are processed equally. The unique thermal block design of Prime Pro 48 achieves this with a
    unique heating and cooling system that quickly cycles from one temperature to the next and then achieves a uniform ±0.1°C in every well of the block within a fraction of a second of reaching each dwell temperature. No other block-based qPCR system can achieve similar performance.

    The capacity of Prime Pro 48 accommodates a unique and economical 48-well PCR plate, the size of which is only 1/8 the size of a standard 96-well plate. The well format mirrors a 384-well plate therefore allowing the use of a 16-channel micropipette. The small plate cuts reagent costs in half whilst still producing a strong fluorescence signal. Minimizing the plate size also significantly improves thermal uniformity.

    Technical Specification

    High Resolution Melt

    Yes

    Volume per well

    Validated for 5 to 20 ul

    Detection sensitivity

    1 copy

    Temp uniformity

    +- 0.1 °C within 1 sec

    Temp stability

    +- 0.1 °C

    Temperature range

    30 to 100 °C

    Average ramp rate

    5.5 °C sec

    Thermal system

    Hollow silver block peltier-based with conductive fluid

    Block format

    48-well block

    Consumables

    48-well custom plates and optical adhesive seals

    Optical system

    Dual LED excitation (452 to 486 nm and 542 to 582 nm)

    Camera

    CCD camera

    Filters

    4 emission filters (505 to 545nm 562 to 596nm 604 to 644nm 665 to 705nm)

    Calibrated dyes

    SYBR FAM HEX ROX Cy5

    Additional dyes

    All dyes within range compatible with no additional calibration

    Passive reference dyes

    Use of ROX is supported but optional

    Data collection

    Data always collected in all four filters for all wells

    Plate setup

    Plate setup for data analysis can be altered after run completes

    Melt curve

    Supports continuous data acquisition in a single filter to provide increased data point collection and reduced run times

    PCR cycle time

    40 cycles in less than 40 minutes

    PCR cycle time (FAST optimized)

    40 cycles in less than 20 minutes

    Dynamic range

    Greater than 9 logs

    Calibration

    Not required

    Installation

    Plug and play design

    Precision

    Discriminates 5000 and 10000 template copies with 99 percent confidence

    Warranty

    1 year (parts and labor included)

    Voltage

    120 to 240 V

    Frequency

    50 and 60 Hz

    Nominal current draw

    8A

    Peak power

    500W (typical power is 180W)

    Software

    Multiple license software included at no additional cost

    Chemistry

    All chemistries supported

    Applications

    Absolute Quantification and Relative Quantification

    Applications

    Allelic Discrimination and High Resolution Melt

    Dimensions closed (WxDxH)

    34.5cm x 31cm x 32cm (13.6 in x 12.2 in x 12.6 inches)

    Dimensions open (WxDxH)

    34.5cm x 31cm x 37cm (13.6 in x 12.2 in x 14.5 inches)

    Weight

    13.6 Kg (30 lb)

     

     

    Ordering information

    Part number

    Description

    PRIMEPRO48

    Prime Pro 48 Real Time PCR System, includes Accessories and Software (Pro and ProControl). PC not supplied

    PROPLATE48

    Pack of 50 Real Time PCR Plates, 48-wells

    PROSEAL48

    Pack of 50 Real Time PCR Plate seals

SKU Number Type # of Samples Web Price Quantity Add to Cart
PRIMEPRO48 Thermal cycler N/A
21322.00
Each
PROPLATE48 Plates 50
101.94
Each
PROSEAL48 Seals 48
82.98
Each
Prime Pro 48 Real Time PC
Prime Pro 48 Real Time PCR system
0.0000
Each
On Order
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