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PCR/qPCR
MIDSCI can supply almost every needed product for PCR/qPCR: tubes, plates, strips, caps, taq, Mastermixes, dNPTs, dye and probe for qPCR, sealing film and much more. If you are looking for any of these things, we can match almost all needs to all cyclers. If you don’t find it easily contact us at tech@midsci.com and we will find it for you.
96 or 384 we have it. Non-Skirted, semi-skirted or full skirted, we have it. 0.1, 0.2, or 0.5 mL we have it. A1, A12, H12, A24, P24 cuts, we have them. Sybr© Green or probe alternative, we have it.
Pro Tip: Colored plastics do not have any impact on DNA amplification. But when setting up qPCR it is often recommended to use white plastics. This will limit or prevent fluorescence refraction. Light refraction can minimize sensitivity and consistency.
Pro Tip 2: If you are seeing inconsistencies in PCR/qPCR try PR1MA Pipette Tips. This will help optimize and reduce variance in the reactions. Simple test, see the residual liquid left in tips? This means some of the taq, dNTPs, sample, etc is left in the tip. PR1MA tips help reduce that significantly and improve your consistency.
Pro Tip 3: Seeing evaporation in the outer wells of a PCR/qPCR plate? Sometimes it’s the plate, sometimes it the seal, and sometimes it’s the cycler. Call us we have solutions.
- Safely excise bands from gels
- Avoid cross contamination
- One-handed operation for quick and accurate gel cutting
- Safer than using razor blades and won't scratch transilluminators
Cut gel with pipet tip on the end of a standard 1000µl pipettor. Gel piece is suspended in tip when pipettor is lifted from the gel. Expel the gel piece by pushing the pushbutton on the pipettor. Tip is ejected in normally using the ejector button.
Combo Includes:
200 μL Taq DNA Polymerase (5 units/μL)
4 x 500 μL vials of dNTP Premix (100 mM each of dATP, dCTP, dGTP, dTTP)
D118-1000
High-performance Taq DNA Polymerase specially purified to produce excellent yields with little or no background. Its outstanding activity and unique thermal DNA amplification properties make it one of the most economic DNA polymerases available.
Special Features
- Robust performance
- Leaves 3'A overhang
- Stable at all storage temperatures
- Best price/quality
- APPLICATIONS
- General PCR
- Genomic analysis
- TA cloning
D116
Ready-to-use, ultra-pure, molecular grade solution made from 100mM each of dATP, dCTP, dGTP, and dTTP stock. The PreMix is designed to reduce "hands-on" time for researchers, thereby lowering the possibility of contamination and error. Furthermore, every lot of dNTP solution is pre-tested for optimal performance. The nucleotides are supplied in sterile, doubly distilled water. Each dNTP has a final concentration of 10mM for a total PreMix dNTP concentration of 40mM.
Special Features
- Ready to use
- Each lot functionally tested
- Purity; at least >99%
- APPLICATIONS
- For RT-PCR, large PCR, and real-time PCR
- Reverse Transcription and mutagenesis
PR1MA™ RNA Controls
Looking for safe assay controls for highly infectious viruses or foreign animal diseases?
MIDSCI™ offers a selection of PR1MA™ RNAs – nuclease-resistant, single-stranded RNAs, suitable as process controls for RNA extraction from various sample matrices. These specially engineered, non-infectious, MS2 phage-like particles protect their contents from degradation by nucleases and can package sequences of up to 1.5 kb from viruses such as SARS-CoV-2, foot-and-mouth disease virus, and human immunodeficiency virus.
Amount: Between 1E6 or 1E10 copies (cp)
Concentration: Between 1E7 or 1E10 cp / mL, respectively
Volume: 0.1 and 1 mL respectively
Lysis: 65° C for 5 minutes in the RT step or by standard RNA extraction
Storage temperature: 4°C
Buffer composition
- 10 mM Tris HCl
- 100 mM NaCl
- 1 mM MgCl2
- 0.1% gelatin
- pH = 7.0
*These products are intended for research use only, not for therapeutic or diagnostic purposes in humans or animals. The safety and efficacy of these products in diagnostic or other clinical uses has not been established.
SealPlate® Sealing Film - The Industry Standard for Sample Protection
SealPlate® minimizes evaporation, prevents well-to-well contamination and spillage. Eliminates the “edge effect” which can occur with plate lids (e.g. greater evaporation in the outside plate wells).
- Provides a secure seal and is easy to remove
- Rigid polyester film features good clarity and easy handling
- Can be used as a temporary economical seal while awaiting further processing
- Features two end-tabs with perforations
Applications
- General laboratory use
- Short-term storage
- Incubation
- ELISA
- -40°C to +120°C
Qamp Mini Thermal Cycler
- Compact in Size: 102 x 136 x 104 mm
- Preprogramed Chip: with unlimited applications
- Touch Start: One Button to Go!
- LCD Display: for customers to define status easily!
- Portable: Only 1 kg
Specifications:
| Sample Number | 8 (2 x 4 wells) |
| Temperature Range | 4°C - 99°C |
| Max Heating Rate (°C/sec.) | 4.6°C |
| Max Cooling Rate (°C/sec.) | 3.4°C |
| Temperature Accuracy | ± 0.4°C |
| Temperature Uniformity Across Block | ± 0.4°C |
| Max No. of Cycles | unlimited |
| Max No. of Steps | unlimited |
| Display | LCD |
| Heated Lid | 105°C Fixed (pre-heat to 60°C) |
| Dimensions (L x W x H) | 10 x 13 x 10 cm |
| Weight | 1 Kg |
| Power | VAC 100-240, 50/60 Hz, 120 W |
Temperature Range: -20°C to +120°C
Product Info
- Features chamfered corners to eliminate plate overhang for robotics applications and tight storage conditions.
- Certified DNase- & RNase- free
- Next-generation adhesive formula provides better than-ever sample protection with improved evaporation rates
- Standard cut is ideal for use with raised-rim and non-skirted PCR plates
- Large cut is designed for greater plate coverage and easier positioning of the film to the plate
- Large cut is recommended for use with flat-top, semi skirted and full-skirted PCR plates as well as deep well plates
- NOT recommended for qPCR
PR1MA™ Bst DNA Polymerase
PR1MA™ Bst polymerase (patent pending) is a recombinant, truncated, thermostable Bacillus stearothermophilus DNA polymerase with high reverse transcriptase and strand-displacement activities, ideal for isothermal amplification of RNA and DNA targets. PR1MA™ Bst polymerase has increased sensitivity and speed relative to other Bst polymerases and can incorporate dUTP.
Use PR1MA™ Bst polymerase to develop LAMP assays with high sensitivity and specificity.
- Lacks 5’ to 3’ exonuclease activity
- Only Bst polymerase in the market with robust RT and DNA polymerase activity
- Thermostable, working temperature range 64 - 72°C
- Tolerant to inhibitors
- Storage temperature: - 20°C
Buffer composition
- 50% glycerol
- 50 mM Tris-HCl
- 50 mM KCls
- 1 mM DTT
- 0.1 mM EDTA
- 0.05% Tween – 20
- 0.05% NP - 40 substitute
- pH = 7.5
Important note: Please be sure to use the buffer provided with this product to ensure optimal results.
*These products are intended for research use only, not for therapeutic or diagnostic purposes in humans or animals. The safety and efficacy of these products in diagnostic or other clinical uses has not been established.
AlumaSeal® II™ Easy-to-pierce Aluminum Foil Seal
- Certified DNase- & RNase- free
- Excellent chemical resistance (e.g. DMSO)
- Easily pierceable with single or multichannel pipettors and robotic probes
- 1.4mil conformable aluminum foil allows visual verification of the seal’s security around each well
- PCR
- Long-term storage
- Light sensitive assays
- Robotics
- -80°C to +120°C
- End-tab removal before thermal cycling is highly recommended
- Remove diagonally from a corner to prevent tearing
FoilSeal™
- Certified DNase- & RNase- free
- Pierceable with a pipet tip or robotic probe for direct sample recovery
- 2.0mil aluminum foil removes easily without shredding or tearing
- The conformable nature of foil allows for the visual verification of the seal’s security around each well
- Not recommended for long-term storage
- Remove diagonally from a corner to prevent tearing
ThermalSeal® RTS™ Optically Clear, Pressure Activated, Non-tacky Silicone Adhesive
The silicone adhesive is non-tacky until pressure is applied to the sealing surface which then creates a secure bond around the wells and perimeter of the plate. Also ideal for dry samples (e.g. soil, seeds, insects, etc.) because the non-activated adhesive above the wells remains non-tacky. A roller or sealing paddle is highly recommended for use with this product to ensure consistent and adequate pressure is applied during the sealing process.
- Provides an extremely strong seal to minimize evaporation
- Certified DNase- & RNase- free
- Chemically resistant to DMSO, acetonitrile, and methanol
- Ideal for use with flat-top, raised-rim and non-skirted PCR plates
- High optical clarity and low auto-fluorescence
- Available in roll format for automation: ThermalSeal® RTS™ Roll-Seal
- A complimentary sealing paddle is included in each box
Applications
- qPCR
- Long-term storage
- Protein crystallography
- HPLC
- -80°C to +105°C
Helpful Tips
- Sufficient pressure must be applied to activate the silicone adhesive
- Multiple swipes of the sealing paddle in both directions is highly recommended
- A 10 minute or greater wait period before thermal cycling increases the film-to-plate bond
- Safely excise bands from gels
- Avoid cross contamination
- One-handed operation for quick and accurate gel cutting
- Safer than using razor blades and won't scratch transilluminators
Cut gel with pipet tip on the end of a standard 1000µl pipettor. Gel piece is suspended in tip when pipettor is lifted from the gel. Expel the gel piece by pushing the pushbutton on the pipettor. Tip is ejected in normally using the ejector button.
This brand is being discontinued, please contact us for other options.
Looking for other alternatives?
Visit our PR1MA Polymerase page for more options!
Bullseye High Fidelity PCR Master Mix
The newly developed Bullseye HFL PCR Master Mix represents the highest fidelity for a PCR product. Bullseye's HFL PCR Master Mix has eight times higher fidelity when compared to Pfu, previously considered to be the golden standard for high fidelity PCR. This Master Mix also works well for large PCR fragments.
The Bullseye HFL PCR Master Mix is in a 2X format and contains modified, high fidelity thermal stable DNA polymerases, in a pre-optimized PCR buffer. The amplified DNA will be blunt-ended. When using this master mix for most PCR experiments, only template, primers and H2O will be needed.
SPECIAL FEATURES & APPLICATIONS
- High fidelity with robustness
- Large fragment PCR
- 2X master mix - makes PCR easy and consistent
- For cloning, promoter study, RACE, DNA sequencing and more
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