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PCR/qPCR
MIDSCI can supply almost every needed product for PCR/qPCR: tubes, plates, strips, caps, taq, Mastermixes, dNPTs, dye and probe for qPCR, sealing film and much more. If you are looking for any of these things, we can match almost all needs to all cyclers. If you don’t find it easily contact us at tech@midsci.com and we will find it for you.
96 or 384 we have it. Non-Skirted, semi-skirted or full skirted, we have it. 0.1, 0.2, or 0.5 mL we have it. A1, A12, H12, A24, P24 cuts, we have them. Sybr© Green or probe alternative, we have it.
Pro Tip: Colored plastics do not have any impact on DNA amplification. But when setting up qPCR it is often recommended to use white plastics. This will limit or prevent fluorescence refraction. Light refraction can minimize sensitivity and consistency.
Pro Tip 2: If you are seeing inconsistencies in PCR/qPCR try PR1MA Pipette Tips. This will help optimize and reduce variance in the reactions. Simple test, see the residual liquid left in tips? This means some of the taq, dNTPs, sample, etc is left in the tip. PR1MA tips help reduce that significantly and improve your consistency.
Pro Tip 3: Seeing evaporation in the outer wells of a PCR/qPCR plate? Sometimes it’s the plate, sometimes it the seal, and sometimes it’s the cycler. Call us we have solutions.
Temperature Range: -20°C to +120°C
Product Info
- Features chamfered corners to eliminate plate overhang for robotics applications and tight storage conditions.
- Certified DNase- & RNase- free
- Next-generation adhesive formula provides better than-ever sample protection with improved evaporation rates
- Standard cut is ideal for use with raised-rim and non-skirted PCR plates
- Large cut is designed for greater plate coverage and easier positioning of the film to the plate
- Large cut is recommended for use with flat-top, semi skirted and full-skirted PCR plates as well as deep well plates
- NOT recommended for qPCR
AlumaSeal® II™ Easy-to-pierce Aluminum Foil Seal
- Certified DNase- & RNase- free
- Excellent chemical resistance (e.g. DMSO)
- Easily pierceable with single or multichannel pipettors and robotic probes
- 1.4mil conformable aluminum foil allows visual verification of the seal’s security around each well
- PCR
- Long-term storage
- Light sensitive assays
- Robotics
- -80°C to +120°C
- End-tab removal before thermal cycling is highly recommended
- Remove diagonally from a corner to prevent tearing
FoilSeal™
- Certified DNase- & RNase- free
- Pierceable with a pipet tip or robotic probe for direct sample recovery
- 2.0mil aluminum foil removes easily without shredding or tearing
- The conformable nature of foil allows for the visual verification of the seal’s security around each well
- Not recommended for long-term storage
- Remove diagonally from a corner to prevent tearing
ThermalSeal® RTS™ Optically Clear, Pressure Activated, Non-tacky Silicone Adhesive
The silicone adhesive is non-tacky until pressure is applied to the sealing surface which then creates a secure bond around the wells and perimeter of the plate. Also ideal for dry samples (e.g. soil, seeds, insects, etc.) because the non-activated adhesive above the wells remains non-tacky. A roller or sealing paddle is highly recommended for use with this product to ensure consistent and adequate pressure is applied during the sealing process.
- Provides an extremely strong seal to minimize evaporation
- Certified DNase- & RNase- free
- Chemically resistant to DMSO, acetonitrile, and methanol
- Ideal for use with flat-top, raised-rim and non-skirted PCR plates
- High optical clarity and low auto-fluorescence
- Available in roll format for automation: ThermalSeal® RTS™ Roll-Seal
- A complimentary sealing paddle is included in each box
Applications
- qPCR
- Long-term storage
- Protein crystallography
- HPLC
- -80°C to +105°C
Helpful Tips
- Sufficient pressure must be applied to activate the silicone adhesive
- Multiple swipes of the sealing paddle in both directions is highly recommended
- A 10 minute or greater wait period before thermal cycling increases the film-to-plate bond
- Safely excise bands from gels
- Avoid cross contamination
- One-handed operation for quick and accurate gel cutting
- Safer than using razor blades and won't scratch transilluminators
Cut gel with pipet tip on the end of a standard 1000µl pipettor. Gel piece is suspended in tip when pipettor is lifted from the gel. Expel the gel piece by pushing the pushbutton on the pipettor. Tip is ejected in normally using the ejector button.
Bullseye High Fidelity PCR Master Mix
The newly developed Bullseye HFL PCR Master Mix represents the highest fidelity for a PCR product. Bullseye's HFL PCR Master Mix has eight times higher fidelity when compared to Pfu, previously considered to be the golden standard for high fidelity PCR. This Master Mix also works well for large PCR fragments.
The Bullseye HFL PCR Master Mix is in a 2X format and contains modified, high fidelity thermal stable DNA polymerases, in a pre-optimized PCR buffer. The amplified DNA will be blunt-ended. When using this master mix for most PCR experiments, only template, primers and H2O will be needed.
SPECIAL FEATURES & APPLICATIONS
- High fidelity with robustness
- Large fragment PCR
- 2X master mix - makes PCR easy and consistent
- For cloning, promoter study, RACE, DNA sequencing and more
Adhesive Sealing Film, Aluminum, 1.4 mil
Specifications
| Length | 133.35 mm |
| Length (w/ end tabs removed) | 120.65 mm |
| Width | 80.01 mm |
| Overall Thickness | 1.4 mil |
| Adhesive Thickness | 1.1 mil |
| Material | Aluminum |
| Adhesive | Medical Grade Acrylic |
| Max. Temperature | 120 °C |
| Min. Temperature | -80 °C |
| Primary Usages | Storage or Sample Cover |
Specifications
| Length | 141.27 mm |
| Length (w/ end tabs removed) | 117.5 mm |
| Width | 77.8 mm |
| Overall Thickness | 2.8 mil |
| Adhesive Thickness | 2.0 mil |
| Material | Polyester |
| Adhesive | Encapsulated Silicone |
| Max. Temperature | 120 °C |
| Min. Temperature | -40 °C |
| Primary Usages | Real Time PCR (qPCR), PCR |
- Quickly Spin down droplets and condensation
- Use before and after thermal cycling to increase PCR yield
- Accepts skirted, Non-Skirt and all standard PCR plates
- Less than 1/4 the size of most plate centrifuges
- 1 year warranty
| Capacity: | 2 PCR Plates |
| Rotor Speed: | 2500 rpm |
| G Force: | 500 xg |
| Rotor: | Fixed Vertically |
| Dimensions (WxDxH): | 7.5 x 8.2 x 7.2 in. |
| 19 x 21 x 18.3 cm | |
| Weight: | 6 lbs. / 3 kg |
| Electrical: | 120V or 230V, 50-60 Hz |
Mini Plate Spinner Adapter Supports:
- 8 x 0.2ml PCR Strips
- 12 x 0.2ml PCR Strips
- 0.2ml PCR Tubes
- 48 well PCR Plates
Please know that the manufacturer has suspended manufacturing this product indefinitely.
We suggest purchasing PR1MA PCR Plastics.
Contact your rep or email tech@midsci.com for more information.
Platemax CyclerSeal Sealing Film
- Provides exceptional seal to safeguard samples during transport or storage
- Eliminates well to well contamination and cross-over in PCR applications when used with Axygen's compression mat
- Polypropylene film is wide temperature range compatible, -40° to 104°C
- PCR-TS is designed for ELISA/EIA applications
- PCR-TS-900 is twice as thick (as PCR-TS) and is suitable for water-bath and other difficult PCR applications
- Provides exceptional seal under the widest temperature conditions and reagents
- Wide temperature range compatible, -80° to 104°C
- Ideal for light sensitive samples and PCR or storage
- Uniform adhesive provides optimum well sealing
- PCR-AS-200 is pierceable
- 100 films/pack
AccuSeal Semi Automated Plate Sealers from MIDSCI are engineered and constructed by Labnet International, Inc to maximize PCR, assay and storage applications with a user friendly panel and automated sealing options.
Great emphasis has been placed on incorporating a wide range of standard heights to deep well microplates with appropriate adaptors to efficiently fit the plates for consistent temperature regulation. The AccuSeal sealer is optimized for simple digital control and operation, user-friendly versatility and convenience as well as safety.
Controls and Operation:
- Intuitive layout permits optimal sealing time, temperature and settings
- Includes a "No Heat" setting for pressure sealing applications
Versatility and Convenience:
- Adaptors specific for a PCR plate or an assay plate accommodates a variety of fits
- Automatically adjusts heat for different height plates
- Provides uniform pressure for a quality seal with special non-stick coating to prevent messy cleanup.
Safety:
- Motorized drawer features safety switches and prevents injury to the user.
Specifications:
Dimensions |
6.75x12.75x14.25 inches |
Weight |
24 Lbs |
Sealing Temperature |
OFF, 100 to 190 °C |
Temperature Accuracy |
+/- 1.00 °C |
Temperature Uniformity |
+/- 1.00 °C |
Sealing Time |
0.5 to 10 sec |
Compatible Plate Materials |
PP (Polypropylene) |
Compatible Plate Types |
Standard Assay Plates |
Maximum Plate Height |
45mm |
Compatible Sealing Film Types |
Foil-polypropylene laminate |
Adapters
| PS1000-PCR | AccuSeal PCR plate adapter for sealing PCR and 384 well plates |
| PS1000-ADAPT | AccuSeal Assay plate adapter for sealing assay plates |
Catalog Number |
Description of Sealing Film |
PS100-GP111 |
|
PS1000-GP300 |
|
PS1000-100 |
|
PS1000-150 |
|
PS1000-200 |
|
PS1000-300 |
|
PS1000-400 |
|
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