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IBI MIDI I-Blue Plasmid Sample 

The I-Blue MINI Plasmid Kit was designed for rapid isolation of plasmid DNA from 1-7 mL of cultured bacterial cells. I-Blue Lysis Buffer (an optional color indicator) is included with the kit in order to prevent common handling errors, ensuring efficient cell lysis and neutralization. A modified alkaline lysis method and RNase treatment are used to obtain clear cell lysate with minimal genomic DNA and RNA contaminants. Typical yields are 20-35 µg for high-copy number plasmid or 3-10 µg for low-copy number plasmid from 4 mL of cultured bacterial cells. DNA phenol extraction or alcohol precipitation is not required and the entire procedure can be completed within 15 minutes. The purified plasmid DNA is ready for use in restriction enzyme digestion, ligation, PCR, and sequencing reactions.

Advantages

  • Sample: 1-7 mL of cultured bacterial cells
  • Yield: Up to 50 µg of pure plasmid DNA
  • Format: Plasmid spin column
  • Operation Time: Within 15 minutes
  • Elution Volume: 30-100 µL
  • Kit Storage: Dry at room temperature (15-25°C) for up to 1 year; PD1 and RNase A mixture should be stored at 2-8°C for up to 6 months

Quality Control
The quality of the I-Blue MINI Plasmid Kit is tested on a lot-to-lot basis by isolating plasmid DNA from a 4 mL overnight E. coli (DH5α) culture containing plasmid pBluescript (A600 > 2 U/mL). Following the purification process, a yield of more than 20 µg is obtained and the A260/A280 ratio is between 1.8-2.0. The purified plasmid DNA (1 µg) is used in EcoRI digestion, and analyzed by electrophoresis.

Item#:
ASIBISAMPLEBLUE

MagBio HighPrep™ Plasmid DNA Plus Kit


Magnetic beads based kit designed to extract high-quality plasmid DNA (including Cosmids, Fosmids, and BACs) from bacterial cultures.


Applications

Plasmid DNA isolation for:

  •     PCR, Real-time PCR
  •     Cloning, genotyping
  •     Sequencing, NGS
  •     Transfection

Benefits

  •     High quality plasmid DNA purification
  •     High yields of both high and low copy number plasmids
  •     Adaptable to various automated liquid handling workstations
  •     No toxic organic solvents

The HighPrep™ Plasmid DNA kit is a magnetic beads-based plasmid purification system for high throughput rapid isolation of both high and low copy number plasmid DNA from bacterial cultures as well as for isolation of BACs, PACs, Cosmids or Fosmids.  The system can easily be automated on many liquid handling workstations, providing robustness and high-quality plasmid DNA for subsequent downstream applications such as restriction digestion, PCR, cloning and fluorescent DNA sequencing.
Item#:
ASHIHPRPPLADNAPK

The RNase-Free DNase I set ensures complete DNA removal from RNA extracted using IBI's RNA extraction kits for use in DNA sensitive downstream applications. This is an optional treatment as IBI's spin column technology yields RNA with the majority of DNA removed. Without using DNase treatment the extracted RNA can be used in downstream applications which are not DNA sensitive. This set can be used for both in column DNase digestion and DNase digestion in solution.

Item#:
ASDNARNAKIT2

Sample Types:

Blood, cultured cells, body fluids, and other animal cells

Sample Size:

5 x 10 cultured animal cells, 1 x 10 bacterial cells, or 300µl of blood
Format:
Spin columns
Operation:
Centrifuge/Vacuum

Binding Capacity:
Up to 60µg
Time:
30 minutes
Applications:

RT-PCR, Real-Time PCR, Northern Blotting, Primer Extension, RNAse Protection Assay, mRNA Selection, cDNA Synthesis
Item#:
ASDNARNAKIT1

Advantages

  • Sample: Tissue, rodent tails, ear punches, fresh or frozen blood, serum, plasma, buffy coat, body fluids, cultured cells, amniotic fluid, FFPE, hair, insects
  • Yield: Up to 5 µg of gDNA from fresh whole blood samples
  • Format: genomic DNA spin column
  • Operation Time: Within 20 minutes
  • Elution Volume: 30-100 µL
  • Kit Storage: Dry at room temperature (15-25°C) for up to 1 year

Introduction
The gMax Mini Kit is optimized for genomic, mitochondrial and virus DNA purification from whole blood (fresh blood and frozen blood), tissue, formalin-fixed paraffin-embedded tissue (FFPE), amniotic fluid and insects in one convenient kit. This DNA extraction kit uses Proteinase K and chaotropic salt to lyse cells and degrade protein, allowing DNA to bind to the glass fiber matrix of the spin column. Contaminants are removed using a Wash Buffer and the purified genomic DNA is eluted by a low salt Elution Buffer, TE or water. The entire procedure can be completed within 20 minutes without phenol/chloroform extraction or alcohol precipitation. The purified DNA (approximately 20-30 kb) is suitable for use in PCR or other enzymatic reactions.

Quality Control
The quality of the gMax Mini Kit is tested on a lot-to-lot basis by isolating genomic DNA from 200 μL of whole human blood. The purified DNA (5 μg with an A260/A280 ratio of 1.8-2.0) is quantified with a spectrophotometer and analyzed by electrophoresis.

Kit Components


Component

IB47280

IB47281

IB47282

GST Buffer

3 mL

30 mL

75 mL

GSB Buffer

4 mL

40 mL

75 mL

W1 Buffer

2 mL

45 mL

130 mL

Wash Buffer*
(Add Ethanol)

1 mL
(4 mL)

25 mL
(100 mL)

50 mL
(200 mL)

Proteinase K**
(Add ddH2O)

1 mg
(0.10 mL)

11 mg x 2
(1.10 mL)

65 mg
(6.50 mL)

Elution Buffer

1 mL

30 mL

75 mL

GD Columns

4

100

300

2 mL Collection Tubes

8

200

600

*Add absolute ethanol (see the bottle label for volume) to Wash Buffer then mix by shaking for a few seconds. Check the box on the bottle. Be sure and close the bottle tightly after each use to avoid ethanol evaporation.

**Add ddH2O to Proteinase K (see the bottle label for volume) then vortex to ensure Proteinase K is completely dissolved. Check the box on the bottle. Once it is dissolved completely, centrifuge for a few seconds to spin the mixture down. For extended periods, the ddH2O and Proteinase K mixture should be stored at 4°C.

Item#:
ASDNARNAKIT4
  • Sample: Gram (+) positive and Gram (-) negative bacterial cells
  • Yield: Up to 30 µg of gDNA - (1 x 10 Escherichia coli: 25-30 µg, 1 x 10 Bacillus subtilis: 10-15 µg)
  • Convenient: Includes Gram+ Buffer for preparing lysozyme solutions and to speed up sample preparation
  • Format: Genomic DNA spin columns
  • Operation Time: within 30 minutes
  • Elution Volume: 50-200 µL
  • Kit Storage: Dry at room temperature (15-25°C) for up to 1 year

The gBAC Mini DNA Bacteria Kit is optimized for genomic and viral DNA purification from Gram (-) negative and Gram (+) positive bacterial cells. Gram+ Buffer, when combined with lysozyme, will efficiently lyse bacterial cell walls consisting of the peptidoglycan layer. Chaotropic salt is used to further lyse cells and degrade protein, allowing DNA to easily bind to the glass fiber matrix of the spin column. Contaminants are removed using a Wash Buffer (containing ethanol) and the purified genomic DNA is eluted by a low salt Elution Buffer, TE or water. Phenol/chloroform extraction or alcohol precipitation is not required and the purified genomic DNA is ready for use in a variety of downstream applications.

Quality Control
The quality of the gBAC Mini DNA Bacteria Kit is tested on a lot-to-lot basis by isolating DNA from Escherichia coli (1x10) culture (OD600=1.3, 1 mL) harvested by centrifugation at 16,000 x g for 1 minute. 10 µL from a 50 µL eluate of purified DNA is analyzed by electrophoresis on a 1% agarose gel.

Items

IB47290

IB47291

IB47292

Gram+ Buffer*

1.5 mL

30 mL

75 mL

GT Buffer

1.5 mL

30 mL

75 mL

GB Buffer

2 mL

40 mL

100 mL

W1 Buffer

2 mL

45 mL

130 mL

Wash Buffer**
(Add Ethanol)

1 mL
(4 mL)

25 mL
(100 mL

50 mL
(200 mL)

Elution Buffer

1 mL

30 mL

75 mL

GD Columns

4

100

300

2 mL Collection Tubes

8

200

600

*Add lysozyme to Gram+ Buffer immediately prior to use. Once lysozyme is mixed with Gram+ Buffer, the solution can be stored for 2 weeks at 4°C.


**Add absolute ethanol (see the bottle label for volume) to Wash Buffer then mix by shaking for a few seconds. Check the box on the bottle. Be sure and close the bottle tightly after each use to avoid ethanol evaporation.

Item#:
ASDNARNAKIT3


IBI Gel/PCR Fragment Extraction Kits

 

The Large DNA Fragments Extraction Kit was designed to recover or concentrate DNA fragments (> 8 Kb) from agarose gel in 4 easy steps. Salts and enzymes can be effectively removed from the reaction mixture without phenol extraction. Typically, recoveries are up to 85% for Gel Extraction. The entire procedure can be completed in 45 minutes and the DNA is ready for use in PCR, Fluorescent or Radioactive Sequencing, Restriction Enzyme Digestion, DNA Labeling and Ligation.

 

Specifications   

Sample
agarose gel
Recoveryup to 85%
Formatspin column
Operation time45 minutes
Item#:
ASDNARNAKIT9

Efficient Gel Extraction and PCR Clean-up

The Gel/PCR DNA Fragments Extraction Kits are designed to recover or concentrate DNA fragments (50bp-10kb) from agarose gel, PCR reaction, or any other enzymatic reaction. This method uses a chaotropic salt and guanidine thiocyanate to dissolve the agarose gel and denature the enzymes. The DNA fragments in the chaotropic salt are bound to the glass-fiber matrix of the spin column. After washing off the contaminants, the purified DNA fragments are eluted by low salt elution buffer or water. Salts, enzymes, and unincorporated nucleotides can be effectively removed from the reaction mixture without phenol extraction or alcohol precipitation.

Sample:
up to 300mg agarose gel slice
up to 100µl PCR product or other enzymatic reaction
Format:
Spin columns
Operation:
Centrifuge/Vacuum Manifold
Binding Capacity:
10µg DNA
Expectant Yield:
80-90% for gel extraction
90-95% for PCR clean-up
Operational Time: 15min. for PCR clean-up/20min. for gel extraction
Applications:
PCR, Fluorescent or Radioactive Sequencing, Restriction Digests, DNA Labeling, Ligations
Item#:
ASDNARNAKIT6

Advantages

  • Sample: Tissue, rodent tails, ear punches, fresh or frozen blood, serum, plasma, buffy coat, body fluids, cultured cells, amniotic fluid, FFPE, hair, insects
  • Yield: Up to 5 µg of gDNA from fresh whole blood samples
  • Format: genomic DNA spin column
  • Operation Time: Within 20 minutes
  • Elution Volume: 30-100 µL
  • Kit Storage: Dry at room temperature (15-25°C) for up to 1 year

Introduction
The gMax Mini Kit is optimized for genomic, mitochondrial and virus DNA purification from whole blood (fresh blood and frozen blood), tissue, formalin-fixed paraffin-embedded tissue (FFPE), amniotic fluid and insects in one convenient kit. This DNA extraction kit uses Proteinase K and chaotropic salt to lyse cells and degrade protein, allowing DNA to bind to the glass fiber matrix of the spin column. Contaminants are removed using a Wash Buffer and the purified genomic DNA is eluted by a low salt Elution Buffer, TE or water. The entire procedure can be completed within 20 minutes without phenol/chloroform extraction or alcohol precipitation. The purified DNA (approximately 20-30 kb) is suitable for use in PCR or other enzymatic reactions.

Quality Control
The quality of the gMax Mini Kit is tested on a lot-to-lot basis by isolating genomic DNA from 200 μL of whole human blood. The purified DNA (5 μg with an A260/A280 ratio of 1.8-2.0) is quantified with a spectrophotometer and analyzed by electrophoresis.

Kit Components


Component

IB47280

IB47281

IB47282

GST Buffer

3 mL

30 mL

75 mL

GSB Buffer

4 mL

40 mL

75 mL

W1 Buffer

2 mL

45 mL

130 mL

Wash Buffer*
(Add Ethanol)

1 mL
(4 mL)

25 mL
(100 mL)

50 mL
(200 mL)

Proteinase K**
(Add ddH2O)

1 mg
(0.10 mL)

11 mg x 2
(1.10 mL)

65 mg
(6.50 mL)

Elution Buffer

1 mL

30 mL

75 mL

GD Columns

4

100

300

2 mL Collection Tubes

8

200

600

*Add absolute ethanol (see the bottle label for volume) to Wash Buffer then mix by shaking for a few seconds. Check the box on the bottle. Be sure and close the bottle tightly after each use to avoid ethanol evaporation.

**Add ddH2O to Proteinase K (see the bottle label for volume) then vortex to ensure Proteinase K is completely dissolved. Check the box on the bottle. Once it is dissolved completely, centrifuge for a few seconds to spin the mixture down. For extended periods, the ddH2O and Proteinase K mixture should be stored at 4°C.

Item#:
ASDNARNAKIT5

IBI MINI miRNA Isolation

The miRNA Isolation Kits are designed for purification of micro RNA (miRNA) and other small cellular RNAs from tissue samples or cultured cells. Purification of miRNA allows research into biological significant pathways for gene regulation. The standard protocol for isolating total RNA and mRNA are not optimized for isolation of small RNA molecules and result in the loss of substantial amounts of miRNA and other small RNA. 

In addition, removal of the predominantly larger RNAs is required for accurate analysis of miRNA expression by qPCR or microarray analysis. This kit is specifically designed for purification of small RNA with minimal contamination from large RNA molecules or genomic DNA. The method employs a spin column with a silica-based fiber matrix that binds RNA in the presence of a chaotropic salt. The method is based on the selective binding of RNA molecules of various sizes to the silica-based fiber matrix when different ethanol concentrations are present in the solvent.

Item#:
ASIBIMINIMIRNAB

IBI MINI miRNA Isolation

The miRNA Isolation Kits are designed for purification of micro RNA (miRNA) and other small cellular RNAs from tissue samples or cultured cells. Purification of miRNA allows research into biological significant pathways for gene regulation. The standard protocol for isolating total RNA and mRNA are not optimized for isolation of small RNA molecules and result in the loss of substantial amounts of miRNA and other small RNA. 

In addition, removal of the predominantly larger RNAs is required for accurate analysis of miRNA expression by qPCR or microarray analysis. This kit is specifically designed for purification of small RNA with minimal contamination from large RNA molecules or genomic DNA. The method employs a spin column with a silica-based fiber matrix that binds RNA in the presence of a chaotropic salt. The method is based on the selective binding of RNA molecules of various sizes to the silica-based fiber matrix when different ethanol concentrations are present in the solvent.
Item#:
ASIBIMINIMIRNAA

MagBio HighPrep™ Total RNA Plus Kit


Magnetic bead-based kit designed to extract high-quality total RNA from mammalian tissues and cultured cells.


Applications

Total RNA isolation for:

  •     RT-qPCR
  •     RNA sequencing
  •     cDNA synthesis
  •     Gene expression analysis

Benefits

  •     High yield and quality total RNA purification
  •     Adaptable to various automated liquid handling workstations
  •     No toxic organic solvents


The HighPrep™ Total RNA Plus kit is a paramagnetic bead-based system that allows for a fast and efficient purification of high-quality total RNA from tissues and cultured cells suitable for numerous high-performance RNA downstream applications such as RT-PCR, microarrays, cDNA synthesis. As a paramagnetic bead-based system, the HighPrep™ Total RNA kit can be used in a high-throughput format, hence adaptable to numerous commercially available workstations.
Item#:
ASHIHPRPTOTRNAPK
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