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ActionsFast and Economical Plasmid Minipreps
- Ideal for purification from bacterial culture
- Up to 30ug binding capacity
- Expected yield: 20-30ug for high-copy plasmid / 3-10ug for low-copy plasmid
- Perfect for cleanup prior to sequencing and cloning
The IBI High-Speed Plasmid Mini Kits are designed for the rapid isolation of plasmid or cosmid DNA from 1-4ml of bacterial cultures. The modified alkaline lysis method and RNAse treatment are used to create cleared cell lysate with minimal genomic DNA and RNA contaminants. In the presence of chaotropic salt, the plasmid DNA in the lysate binds to the glass fiber matrix in the spin column. The purified plasmid DNA is eluted by a low salt elution buffer or water. This procedure does not require DNA phenol-extraction or alcohol precipitation.
Sample: |
1-4ml bacterial culture |
Format: |
Spin columns |
Operation: |
Centrifuge/Vacuum Manifold |
Binding Capacity: |
up to 30µg |
Expectant Yield: |
20-30µg for high-copy plasmid
3-10µg for low-copy plasmid |
Operational Time: | 30 min. |
Applications: |
PCR, Fluorescent or Radioactive Sequencing, Restriction Digests, DNA Labeling, Ligations |
The I-Blue MINI Plasmid Kit was designed for rapid isolation of plasmid DNA from 1-7 mL of cultured bacterial cells. I-Blue Lysis Buffer (an optional color indicator) is included with the kit in order to prevent common handling errors, ensuring efficient cell lysis and neutralization. A modified alkaline lysis method and RNase treatment are used to obtain clear cell lysate with minimal genomic DNA and RNA contaminants. Typical yields are 20-35 µg for high-copy number plasmid or 3-10 µg for low-copy number plasmid from 4 mL of cultured bacterial cells. DNA phenol extraction or alcohol precipitation is not required and the entire procedure can be completed within 15 minutes. The purified plasmid DNA is ready for use in restriction enzyme digestion, ligation, PCR, and sequencing reactions.
Advantages
- Sample: 1-7 mL of cultured bacterial cells
- Yield: Up to 50 µg of pure plasmid DNA
- Format: Plasmid spin column
- Operation Time: Within 15 minutes
- Elution Volume: 30-100 µL
- Kit Storage: Dry at room temperature (15-25°C) for up to 1 year; PD1 and RNase A mixture should be stored at 2-8°C for up to 6 months
Quality Control
The quality of the I-Blue MINI Plasmid Kit is tested on a lot-to-lot basis by isolating plasmid DNA from a 4 mL overnight E. coli (DH5α) culture containing plasmid pBluescript (A600 > 2 U/mL). Following the purification process, a yield of more than 20 µg is obtained and the A260/A280 ratio is between 1.8-2.0. The purified plasmid DNA (1 µg) is used in EcoRI digestion, and analyzed by electrophoresis.
Kit Components
Component |
IB47170 |
IB47171 |
IB47172 |
PD1 Buffer* |
1 mL |
25 mL |
65 mL |
PD2 Buffer** |
1 mL |
25 mL |
75 mL |
PD3 Buffer |
1.5 mL |
45 mL |
100 mL |
I-Blue Lysis Buffer |
10 µL |
250 µL |
650 µL |
W1 Buffer |
2 mL |
45 mL |
130 mL |
Wash Buffer*** |
1 mL |
25 mL |
50 mL |
Elution Buffer |
1 mL |
6 mL |
30 mL |
RNase A (50 mg/mL) |
Added |
100 µL |
260 µL |
PD Columns |
4 |
100 |
300 |
2 mL Collection Tubes |
4 |
100 |
300 |
*For IB47171 and IB47172 add provided RNase A to PD1 Buffer then mix by shaking for a few seconds. Check the box on the bottle. PD1 and RNase A mixture should be stored at 2-8°C for up to 6 months. For IB47170 samples, RNase A was already added to PD1.
**If precipitates have formed in PD2 Buffer, warm the buffer in a 37°C water bath, followed by gentle shaking to dissolve.
***Add absolute ethanol (see the bottle label for volume) to Wash Buffer then mix by shaking for a few seconds. Check the box on the bottle. Be sure and close the bottle tightly after each use to avoid ethanol evaporation.
The Fast Ion Plasmid Maxi Kit (Endotoxin Free) uses pre-packed anion exchange resin columns to purify plasmid DNA from 100-250 ml of bacterial cultures. Modified alkaline lysis method (1) and RNase treatment are used for obtaining clear cell lysate with minimal genomic DNA and RNA contaminants. Once the plasmid DNA has been bound to the column, the contaminants can be washed off using the wash buffer. Finally, the purified plasmid DNA is eluted by a high salt buffer and precipitated with isopropanol for desalting. The entire procedure can be completed in 120 minutes and the obtained high purity plasmid DNA is suitable for transfection, sequencing reactions, PCR and in-vitro transcription.
Specifications: |
Endo Free Midi |
Endo Free Maxi |
Sample: |
50ml culture for high-copy |
100ml culture for high-copy |
100ml culture for low-copy |
250ml culture for low-copy |
|
Yield: |
up to 200ug of plasmid DNA |
500ug to 1mg of plasmid DNA |
Format: |
gravity flow |
gravity flow |
Time: |
approx 120 min. |
approx 120 min. |
The 96-Well Genomic DNA Kits are designed for high-throughput purification of total DNA (including genomic, mitochondrial and viral DNA) from whole blood and a variety of animal tissues or cells. This method uses Proteinase K and a chaotropic salt to lyse cells and degrade proteins. DNA in the chaotropic salt is bound by the glass fiber matrix of each well. Once any contaminants have been removed, the purified DNA is eluted by a low salt elution buffer or water. The entire procedure can be completed in 1 hour without phenol extraction or alcohol precipitation. These kits can be used for manual filtration or with robotic handling systems, and purified DNA with approximately 20-30kb is suitable for PCR or other enzymatic reactions.
Sample Size: |
Up to 25mg of animal tissues, mouse tails or swabs |
Format: |
96-Well Plates |
Operation: |
Centrifuge/Vacuum manifold |
Binding Capacity: |
Up to 30ug per well |
Operation Time: |
60 minutes |
The Genomic DNA Mini Kit for Plants provide a quick and easy method for purifying total DNA (including genomic DNA, mitochondrial and chloroplast DNA) from plant tissue. Samples are disrupted by both grinding in liquid nitrogen and lysis buffer incubation. The lysate is treated with RNase A to degrade RNA and then filtered to remove cell debris and salt precipitates. In the presence of the binding buffer, coupled with chaotropic salt, genomic DNA in the lysate binds to the glass fiber matrix of the spin column. Contaminants are removed using a Wash Buffer (containing ethanol) and the purified genomic DNA is eluted by a low salt Elution Buffer or TE. The procedure does not require DNA phenol extraction or alcohol precipitation, and can be completed in less than 1 hour. The purified genomic DNA is ready for use in PCR, Real-time PCR, Southern Blotting and RFLP.
|
MINI |
MAXI |
Sample Size: |
up to 100mg fresh plant tissue/ |
up to 1g of fresh plant tissue/ |
Format: |
Spin Column |
Spin Column |
Expectant Yield: |
up to 50µ g DNA |
up to 140µ g DNA |
Operation Time: |
60 min or less |
60 min |
The Genomic DNA Mini Kit for Tissue was designed specifically for purifying total DNA (including genomic, mitochondrial and viral DNA) from a variety of animal tissue, paraffin-embedded tissue, buccal swab and amniotic fluid. The provided micropestle can efficiently homogenize tissue samples to shorten the time in the Lysis Step. Proteinase K and chaotropic salt are used to lyse cells and degrade protein, allowing DNA to be easily bound by the glass fiber matrix of the spin column. Once any contaminants have been removed, using a Wash Buffer (containing ethanol), the purified DNA is eluted by a low salt Elution Buffer or TE. The entire procedure can be completed within 1 hour without phenol/chloroform extraction or alcohol precipitation. The expected yield of genomic DNA is up to 50µg and the purified DNA (with approximately 20-30 Kb) is suitable for use in PCR or other enzymatic reactions.
Sample Size: |
Up to 50mg of tissue or 200ul of blood |
Format: |
Spin Column |
Expectant Yield: |
up to 50µ g DNA in 50-200µ l |
Operation Time: |
60 min or less |
The Genomic DNA Kit for Blood or Cultured Cells provides an efficient method for purifying total DNA (including genomic, mitochondrial and viral DNA) from whole blood, frozen blood, buffy coat, cultured animal/bacterial cells and fungus. Chaotropic salt is used to lyse cells and degrade protein, allowing DNA to bind to the glass fiber matrix of the spin column. Contaminants are removed using a Wash Buffer (containing ethanol) and the purified genomic DNA is eluted by a low salt Elution Buffer or TE. The entire procedure can be completed in 1 hour without phenol/chloroform extraction or alcohol precipitation, with an average DNA yield of 6 µ g from 200 µ l of whole human blood and up to 50µ g of DNA from 200µ l of buffy coat. Purified DNA, with approximately 20-30 Kb, is suitable for use in PCR or other enzymatic reactions.
|
MINI |
MAXI |
Sample Size: |
up to 300µ l fresh whole blood, up to 10 cultured cells |
10ml frozen blood, up to 10 cultured cells |
Format: |
Spin Column |
Spin Column |
Expectant Yield: |
up to 50µ g DNA in 50-200µ l |
up to 140µ g DNA in 1-2ml |
Operation Time: |
40 min or less |
60 min or less |
MagBio HighPrep™ Viral/Bacterial DNA/RNA Kit
Magnetic beads based kit for rapid isolation of viral and bacterial nucleic acids from whole blood, serum, plasma, saliva and other body fluids.
- RT-qPCR; RT-PCR, PCR
- One-Step RT-qPCR
- Virus detection, genotyping
- Viral load monitoring,
- OPTIMIZED FOR ISOLATION from BACTERIAL & VIRAL samples.
- Rapid and reliable purification of nucleic acids
- Adaptable to various automated liquid handling workstations
- No toxic organic solvents
, Beads
IsoPure™ 96 & IsoPure™ Mini | Automated Extraction Systems
IsoPure™ 96
- Operates as a stand-alone system, or connect to PC for programming
- 8-position carousel for deep well sample plates
- Intuitive programming and operation on a user-friendly touch screen
- Reliable and automated processing of up to 96 samples
The IsoPure™ 96 from Accuris Instruments is a high throughput automated extraction system that improves efficiency and reproducibility of magnetic bead extractions. Capable of processing up to 96 samples at one time, the instrument is easy to set up and program. Mixing, magnetic bead transfer, washing and elution steps are performed automatically, saving valuable hands-on time. The complete purification process can be finished in as little as 10 minutes (depending on the reagent kit and complexity of the program.)
IsoPure™ Mini
- Compact design takes up only 8 x 10 inches of bench space
- Intuitive programming on a user-friendly android tablet app
- Higher yields and reproducibility than manual extraction methods
- Fast and reliable sample processing
The IsoPure™ Mini from Accuris Instruments is an extremely compact,
automated extraction system that improves efficiency and reproducibility of
magnetic bead extractions. Capable of processing up to 16 samples at one
time, the instrument is easy to set up and program. Mixing, magnetic bead
transfer, washing and elution steps are performed automatically, saving
valuable hands-on time. The complete purification process can be finished
in as little as 10 minutes (depending on the reagent kit and complexity of
the program.)
|
IsoPure™ 96 |
IsoPure™ Mini |
Capacity / Sample Volume |
96
Samples / 20 µL to 1000 µL |
16
Samples / 20 µL to 1000 µL |
Magnetic Bead Recovery Rate |
>95% |
>95% |
Lysis Temperature Range |
RT to
120°C |
RT to
120°C |
Elution Temperature Range |
RT to
120°C |
RT to
120°C |
Display |
7.0
inch color screen |
4.3
inch color screen |
Programming Control |
Touch
screen/mouse, or PC connection |
Touch
screen/mouse, or PC connection |
Program Memory |
100
programs |
100
programs |
Communication Ports |
USB,
Ethernet |
USB,
Ethernet |
Contamination Control |
UV
Sterilization |
UV
Sterilization |
Electrical Input |
Universal
120 to 240VAC |
Universal
120 to 240VAC |
Dimensions |
22 x 24.5
x 19.7 in (56 x 62 x 50 cm) |
7.9 x
10.2 x 11.8 in (20 x 26 x 30 cm) |