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ION Biosciences Essentials Red Calcium
Red Calcium Essentials provides the necessary reagents for multi-well plate-based, fluorescence microscopy applications and high-throughput measurements of intracellular calcium (Ca²+) mediated changes through a wide variety of plasma membrane and intracellular calcium channels and transporters. Red Calcium Essentials is also useful for investigating numerous effectors of ion channels and transporters, including G-protein coupled receptors.
Red Calcium Essentials provides the necessary reagents for multi-well plate-based, fluorescence microscopy applications and high-throughput measurements of intracellular calcium (Ca²+) mediated changes through a wide variety of plasma membrane and intracellular calcium channels and transporters. Red Calcium Essentials is also useful for investigating numerous effectors of ion channels and transporters, including G-protein coupled receptors.
Our red calcium dye, ION Calcium Red-1 (ICR-1), is a red fluorescent indicator (Ex/Em: 580/660 nm) responsive to intracellular Ca²+ dynamics. ICR-1’s spectral properties allows for multiplexing with an indicator of another color or GFP-tagged cells.
When following the recommended protocol, Red Calcium Essentials provides enough reagents to make 100 mL of working solution, enough for ten 96-well plates. The actual number of assays will vary according to optimal dye concentrations for your application.
Item#:
ASESSREDCAL
ION Biosciences Fluo-4 K+ Salt
Fluo-4 K+ Salt is a membrane impermeable form of Fluo-4 that can be used in lipid membrane-free systems, in liposomes, or can be introduced into cells by electroporation, microinjection or other methods.
Green fluorescent, calcium (Ca²+) indicator, membrane impermeable.
Fluo-4 is the most popular green fluorescent, calcium (Ca²+) indicator. Proven and trusted for decades to measure intracellular Ca²+ flux in high throughput screening and fluorescence microscopy applications.
Fluo-4 K+ Salt is a membrane impermeable form of Fluo-4 that can be used in lipid membrane-free systems, in liposomes, or can be introduced into cells by electroporation, microinjection or other methods.
Item#:
ASFLUO4AMST
ION Biosciences IPG-1 TMA Salt+
Yellow-green fluorescent, potassium (K+) indicator, membrane
impermeable. Lower K+ affinity than IPG-2 and IPG-4.
ION Potassium Green – 1 (IPG-1) is the best in class yellow-green fluorescent, intracellular potassium (K+) indicator for measuring changes at most intracellular K+ concentrations. IPG-1 has Ex/Em: 525 nm/545 nm and a high-sensitivity to detect small changes in K+ concentration. IPG-1 has a lower affinity (Kd = 50 mM) than IPG-2 (Kd = 18 mM) or IPG-4 (Kd = 7 mM).
IPG-1 TMA+ salt is a membrane impermeable form of IPG-1 that can be used in lipid membrane-free systems, in liposomes, or can be introduced into cells by electroporation, microinjection or other methods.
Item#:
ASIPG1TMAST
ION Biosciences Fura-2 K+ Salt
Ratiometric calcium (Ca²+) indicator, membrane impermeable.
Fura-2 is the most popular UV-excitable, ratiometric green indicator for calcium (Ca²+) measurements. Ex/Em: 340/505 nm can be used to measure Ca²+-bound Fura-2, and Ex/Em: 380/505 nm can be used to detect Ca²+-free Fura-2. Ratiometry is optimal for imaging applications where quantification of intracellular Ca²+ concentrations is desired, and reduces effects of photobleaching, heterogenous dye loading, and variable cell morphology.
Fura-2 K+ salt is a membrane impermeable form of Fura-2 that can be used in lipid membrane-free systems, in liposomes, or can be introduced into cells by electroporation, microinjection or other methods.
Item#:
ASFURA2KST
ION Biosciences ING-2 TMA+ Salt
Yellow-green fluorescent, sodium (Na+) indicator, membrane impermeable. Higher Na+ affinity than ING-1.
ION Natrium Green – 2 (ING-2) is a yellow-green fluorescent, intracellular sodium (Na+) indicator with improved cellular loading and significantly higher brightness than SBFI. Its spectral properties (Ex/Em: 525 nm/545 nm) and large dynamic range make ING-2 the best Na+ indicator for high-throughput screening applications targeting Na+ channels, and non-selective monovalent cation channels. ING-2 has a more physiologically relevant affinity (Kd = 20 mM) than ING-1 (Kd = 92 mM). Also compatible with fluorescence microscopy using common fluorescein, GFP or more ideally YFP filters.
ING-2 TMA+ salt is a membrane impermeable form of ING-2 that can be used in lipid membrane-free systems, in liposomes, or can be introduced
Item#:
ASIGN2TMAST
ION Biosciences Fluo-3 K+ Salt
Green fluorescent, calcium (Ca²+) indicator, membrane impermeable
Fluo-3 is a green fluorescent, intracellular calcium (Ca²+) indicator with slightly red-shifted spectral properties compared to Fluo-4. Offers a large dynamic range that can be better suited for high contrast imaging applications.Is preferred over Fluo-4 for some cell types.
Fluo-3 K+ salt is a membrane impermeable form of Fluo-3 that can be used in lipid membrane-free systems, in liposomes, or can be introduced into cells by electroporation, microinjection or other methods.
Item#:
ASFLUO3AMST
ION Biosciences ICR-1 K+ Salt
ION Calcium Red – 1 (ICR-1) is a red fluorescent (Ex/Em 580nm/660nm) calcium (Ca²) indicator for intracellular Ca²+ measurements. ICR-1’s long-wavelength emission and a large Stokes shift reduces contributions of autofluorescence, making ICR-1 AM optimal for cellular and tissue imaging applications. ICR-1 can also be multiplexed with GFP-labeled cells or other green fluorophores commonly used in experiments. Unlike some other red fluorescent Ca²+ indicators, ICR-1 does not accumulate in the mitochondria. Data supporting fluorescence lifetime imaging and multiphoton imaging capabilities have also been demonstrated.
ION Calcium Red – 1 (ICR-1) is a red fluorescent (Ex/Em 580nm/660nm) calcium (Ca²) indicator for intracellular Ca²+ measurements. ICR-1’s long-wavelength emission and a large Stokes shift reduces contributions of autofluorescence, making ICR-1 AM optimal for cellular and tissue imaging applications. ICR-1 can also be multiplexed with GFP-labeled cells or other green fluorophores commonly used in experiments. Unlike some other red fluorescent Ca²+ indicators, ICR-1 does not accumulate in the mitochondria. Data supporting fluorescence lifetime imaging and multiphoton imaging capabilities have also been demonstrated.
ICR-1 K+ salt is a membrane impermeable form of ICR-1 that can be used in lipid membrane-free systems, in liposomes, or can be introduced into cells by electroporation, microinjection or other methods.
Item#:
ASICR1KST
ION Biosciences IPG-2 TMA+ Salt
Yellow-green fluorescent, potassium (K+) indicator, membrane
Yellow-green fluorescent, potassium (K+) indicator, membrane
impermeable. Lower K+ affinity than IPG-4 and higher affinity than IPG-1
ION Potassium Green – 2 (IPG-2) is a yellow-green fluorescent, intracellular potassium (K+) indicator with Ex/Em: 525 nm/545 nm and a high-sensitivity to detect small changes in K+ concentration. IPG-2 has a higher affinity (Kd = 18 mM) than IPG-1 (Kd = 50 mM) and lower affinity than IPG-4 (Kd = 7 mM).
IPG-2 TMA+ salt is a membrane impermeable form of IPG-2 that can be used in lipid membrane-free systems, in liposomes, or can be introduced into cells by electroporation, microinjection or other methods
Item#:
ASIPG2TMAST
ION Biosciences IPG-4 TMA+ Salt
Yellow-green fluorescent, potassium (K+) indicator, membrane
impermeable. Higher K+ affinity than IPG-1 and IPG-2.
ION Potassium Green – 4 (IPG-4) is a yellow-green fluorescent, intracellular potassium (K+) indicator with Ex/Em: 525 nm/545 nm and a high-sensitivity to detect small changes in K+ concentration. IPG-4 has the highest affinity (Kd = 7 mM) among IPG analogues – IPG-2 (Kd = 18 mM) and IPG-1 (Kd = 50 mM).
IPG-4 TMA+ salt is a membrane impermeable form of IPG-4 that can be used in lipid membrane-free systems, in liposomes, or can be introduced into cells by electroporation, microinjection or other methods. IPG-4 has been successfully used as a sensor for extracellular K+.
Item#:
ASIPG4TMAST
ION Biosciences Fluo-2 K+ Salt
Green fluorescent, calcium (Ca²+) indicator, membrane impermeable. Higher Ca²+ affinity than Fluo-4.
Fluo-2 is a green fluorescent, calcium (Ca²+) indicator. Fluo-2 has higher Ca²+ affinity (Kd = 290 nM), superior cell loading and comparable spectral properties compared to Fluo-4. Offers superior sensitivity versus other Fluo analogues at lower Ca²+ concentrations.
Fluo-2 K+ salt is a membrane impermeable form of Fluo-2 that can be used in lipid membrane-free systems, in liposomes, or can be introduced into cells by electroporation, microinjection or other methods.
Item#:
ASFLUO2AMST
ION Biosciences Fura-2 LR K+ Salt
Leakage-resistant, ratiometric calcium (Ca²+) indicator, membrane impermeable.
UV-excitable, ratiometric green indicator for intracellular calcium (Ca²+) measurements with longer cellular retention compared to Fura-2 AM and similar spectral properties. Ex/Em: 340/505 nm can be used to measure Ca²+-bound Fura-2, and Ex/Em: 380/505 nm can be used to detect Ca²+-free Fura-2. Ratiometry is optimal for imaging applications where quantification of intracellular Ca²+ concentrations is desired, and reduces effects of photobleaching, heterogenous dye loading, and variable cell morphology.
Fura-2 LR K+ salt is a membrane impermeable form of Fura-2 LR that can be used in lipid membrane-free systems, in liposomes, or can be introduced into cells by electroporation, microinjection or other methods.
Item#:
ASFURA2LRKST
ION Vital - Viability Kit
Two-color, fluorescence-based assay to discriminate between live and dead cells.
ION Vital – Viability kit can be used to quantify live and dead cells within eukaryotic cell suspensions or adherent cultures, 3D cultures, organoids, and some non-fixed tissues. Cannot be used for yeast or bacteria. When following our protocol, ION Vital—Viability kit provides enough reagents to make 150 mL of working solution, enough for fifteen 96-well plates or 1,500 flow cytometry samples. The actual number of assays will vary according to optimal dye concentrations for your application.
Item#:
ASAIONVIT
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