ION Biosciences Fura-2 LR AM
 

Leakage-resistant, ratiometric calcium (Ca²+) indicator, membrane permeable.

 

UV-excitable, ratiometric green indicator for intracellular calcium (Ca²+) measurements with longer cellular retention compared to Fura-2 AM and similar spectral properties. Ex/Em: 340/505 nm can be used to measure Ca²+-bound Fura-2, and Ex/Em: 380/505 nm can be used to detect Ca²+-free Fura-2. Ratiometry is optimal for imaging applications where quantification of intracellular Ca²+ concentrations is desired, and reduces effects of photobleaching, heterogenous dye loading, and variable cell morphology.

MDR Activity Kit
 
ION’s Multidrug Resistance (MDR) Activity kit is an effective solution for detecting MDR1 and MRP1 activity and compounds susceptible to MDR–mediated efflux. ION’s MDR Activity kit is compatible with fluorescence microscopy, flow cytometry, and fluorescence plate readers using FITC/fluorescein settings.

Calcein AM is a membrane-permeant, non-fluorescent dye that enters cells passively. Once inside the cytosol of cells, intracellular esterases convert it to fluorescent Calcein (Ex/Em: 495 nm/515 nm), resulting in uniform cytosolic fluorescence. Drug efflux transporters, such as P-glycoprotein (Pgp, MDR1) and multidrug-resistance-associated protein (MRP1), actively extrude Calcein AM from inside the cell before esterases can convert it to Calcein. The presence of additional MDR substrates or inhibitors of MDR expression results in decreased Calcein AM efflux, causing a measurable increase in intracellular fluorescence. In addition to identifying MDR substrates and inhibitors, this kit can also be used to evaluate the activity of MDR transporters in cells.

When following our protocol, ION’s MDR Activity kit provides enough reagents to make 100 mL of working solution, enough for ten 96- or 384-well plates or 80 flow cytometry samples. The actual number of assays will vary according to optimal dye concentrations for your application.

ION VITAL – MitoVolt
 

Mitochondrial membrane potential assay kit that uses JC-10, a membrane potential sensitive dye that is more soluble than JC-1.
 

ION Vital – MitoVolt assay kit is the ideal solution for detecting changes in mitochondrial membrane potential due to cell apoptosis or other stress-inducing phenomena. ION Vital – MitoVolt is compatible with fluorescence microscopy, flow cytometry, and plate reader applications.
 

JC-10 is a mitochondrial membrane potential probe. It possesses superior aqueous solubility compared to its better known analogue, JC-1. At low concentrations, JC-10 is monomeric and emits a green fluorescence.
JC-10 accumulates in healthy mitochondria, forming J-aggregates that exhibit an orange fluorescence. Mitochondrial depolarization, a key marker of cellular apoptosis, results in a loss of JC-10 accumulation and a reversal to its monomeric form. This reversible behavior of JC-10 allows for ratiometric analysis of mitochondrial membrane potential, where a shift from orange (Ex/Em: 540nm/590nm) to green fluorescence (Ex/Em: 490nm/525nm) is indicative of compromised mitochondria.
 

ION Vital – MitoVolt can be used to monitor and/or visualize mitochondrial membrane potential as a static endpoint or in real-time. When following our protocol, ION Vital – MitoVolt provides enough reagents to make 25 mL of working solution, enough for five 96-well plates or 500 flow cytometry samples. The actual number of assays will vary according to optimal dye concentrations and assay volumes for your application.

ION Biosciences BAPTA AM
 

Non-fluorescent calcium (Ca²+) chelator, membrane permeable.

 

BAPTA AM is a highly selective, intracellular calcium (Ca²+) chelator that is less sensitive to pH fluctuations than EGTA and EDTA. Can be used to modulate the level of intracellular Ca²+.

Calcein AM
 

Green fluorescent, live cell indicator.
 

Calcein AM is a membrane-permeant, non-fluorescent form of calcein that is converted to green fluorescent calcein in viable cells, resulting in uniform cytosolic fluorescence (Ex/Em 495 nm/515 nm). Calcein is well retained within the cytosol of most healthy cells with intact cell membranes.

SBFI AM
 

Ratiometric sodium (Na+) indicator, membrane permeable
 

SBFI is a UV-excitable, ratiometric green indicator for intracellular sodium (Na+) measurements. Ex/Em: 340/505 nm can be used to measure Na+-bound SBFI, and Ex/Em: 380/505 nm can be used to detect Na+-free SBFI. It is ~18X more selective for Na+ over K+. Ratiometry is optimal for imaging applications where quantification of intracellular Na+ concentrations is desired, and reduces effects of photobleaching, heterogenous dye loading, and variable cell morphology.

Thallos AM
 

Best in class, green fluorescent, thallium (Tl+) indicator, membrane permeable. Works with potassium (K+), sodium (Na+), and monovalent cation channels, and transporters.
 

Thallos is a green fluorescent, intracellular thallium (Tl+) indicator, and has been the gold standard for fluorescence-based potassium (K+) channel HTS for nearly 2 decades. Thallos also delivers outstanding results for a wide variety of monovalent cation (sodium – Na+) channels, transporters, and GPCRs.

Yellow-green fluorescent, potassium (K+) indicator, membrane permeable.
 

Higher K+ affinity than IPG-1 and IPG-2
 

SBFI K+ SALT
 

Ratiometric sodium (Na+) indicator, membrane impermeable.
 

SBFI is a UV-excitable, ratiometric green indicator for intracellular sodium (Na+) measurements. Ex/Em: 340/505 nm can be used to measure Na+-bound SBFI, and Ex/Em: 380/505 nm can be used to detect Na+-free SBFI. It is ~18X more selective for Na+ over K+. Ratiometry is optimal for imaging applications where quantification of intracellular Na+ concentrations is desired, and reduces effects of photobleaching, heterogenous dye loading, and variable cell morphology.

SBFI K+ salt is a membrane impermeable form of SBFI that can be used in lipid membrane-free systems, in liposomes, or can be introduced into cells by electroporation, microinjection or other methods.

ION Biosciences PBFI K+ Salt
 
PBFI is a UV-excitable, ratiometric green indicator for intracellular potassium (K+) measurements. Ex/Em: 340/505 nm can be used to measure K+-bound PBFI, and Ex/Em: 380/505 nm can be used to detect K+-free PBFI. It is 1.5X more selective for K+ over Naand works well in cytosolic conditions where [K+]/[Na] is ~10. Ratiometry is optimal for imaging applications where quantification of intracellular K+ concentrations is desired and reduces effects of photobleaching, heterogenous dye loading, and variable cell morphology.

PBFI K+ salt is a membrane impermeable form of PBFI that can be used in lipid membrane-free systems, in liposomes, or can be introduced into cells by electroporation, microinjection, or other methods.