TPP Syringe Filters

Key Features:

• Designed for high flow rate of media or aqueous solutions
• 3 to 5 times faster than standard syringe filters
• Typical starting flow rate at 25°C for water at 2bar (~29psi) is 175mL/min
• 0.22µm or µm PES membrane
• Low protein binding
• Larger filter surface
• Low dead Vol Additional specifications
• Higher operating pressure
• Burst pressure 10bar (~145psi)
• Bubble point 3.7bar (~53psi)

• Standard luer-lock connection

• Housing diameter size 33 mm, Syringe filter rests perfectly on a 50mL centrifuge tube

• Gamma-sterilized, DNAse/RNAse-Free, and pyrogen-Free
• Individually wrapped in peel-off plastic wrapper in convenient card box dispenser

Typical applications:

Sterile filtration of protein solutions, tissue culture media, additives, buffers, and water.

Solutions can be filtered fast due to:

• Large filter surface area, that is increasing the flow rate and throuput
• High operating pressure: a maximum housing pressure of 10 bar (~145 psi).

Bi-directional
The syringe filter units bi-directional support of the filter membrane enables users to filter aqueous solutions in either direction (only ONE direction per use):

• Forward (from the syringe into the container)
• Backward (from the container into the syringe)

The filter unit removes micro organisms, particles, precipitates, and undissolved powders larger than 0.22 micron or 0.45 micron, depending on filter used.
 
Specifications: 
 

TPP Cell Scrapers

TPP Cell Scrapers have a pivoting head that enables the blade angle to be changed effortlessly with a slight twist of the fingers while still in contact with the surface being scraped. This allows for easy and thorough cell retrieval from vessels of all shapes and sizes without having to constantly re-position the scraper or culture vessel.  The TPP exclusive raised knobs on the handle allow for a slip free and secure grip when wearing gloves.

Key Features

• Scraper blade
• Pivots for easy maneuvering without constant lifting and re-positioning

• Made from a especially soft and flexible polyethylene material
• Maintains great contact but won't damage cells

• Small raised knobs on handle for a secure grip

• Scraper sizes provide superior access to all edges of culture vessels of all shapes and sizes
• Allows for 100% cell retrieval

• Sterile, individually packaged in a paper/plastic peel-off wrapper
• Packed in stackable and user friendly TPP boxes

• Lint-free blistering packaging

Specifications
 

Item #	Description	Head Width	Quantity
TP99002	Scraper (24 cm)	1.3 cm	150/cs
TP99003	Scraper (30 cm)	2.0 cm	150/cs
TP99004	Scraper (38 cm)	2.5 cm	100/cs
TP99010	Spatula (20 cm)	1.4 cm	150/cs

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We are sorry some of these products have been discontinued.
GREAT NEWS, we have replacements & TPP is working on new pipettes. 

TPP Serologicals were uniquely designed to be shorter and more ergonomic to permit access into vessels of all shapes and sizes, while providing the end user significant advantages in liquid handing and overall usage. The single piece construction of TPP serologicals 1-25mLs eliminate media drip post-aspiration.

Key features:
Standard serological pipettes: TPP 1mL, 2mL, 5mL, 10mL & 25mL Serologicals

• Accurate Vol 1 - 25 ml
• Highly visible, bidirectional (ascending, descending), and over-Vol graduations
• Single piece construction prevents "pipette drip"
• Smooth interiors, prevent cell hang-up or clumping inside the pipette
• Shorter length reduces arm/back strain of end user
• Excellent optical transparency
• Color coded cotton plugs and dispenser boxes for easy identification

• (25mL - Red, 10mL - Orange,  5mL - Blue, 2mL - Green, 1mL - Yellow)

• Convenient, stackable TPP dispenser box
• Single wrapped in lint-free paper/plastic blister

Serological pipettes with reservoir: TPP 25/40mL & 50/70mL Serologicals

• Manufactured with highest grade PS, extremely break resistant and robust
• Reservoir offers large capacity Vols in a compact, ergonomic design. It augments the total Vol capacity without adding proportionate length to the pipette
• Slender and short form enables ergonomic working conditions in the sterile hood
• Small outside diameter of the tube at the tip results in an excellent accessibility of the large pipettes even in small tissue culture flasks

• 40mL and 70mL max Vols enable larger media aspiration

• Optimized form of the mouthpiece preserves the rubber of the pipette aid

The serological pipettes with reservoir from TPP are molded with highest grade polystyrene. They fit in all commercially available pipette aids with rubber inserts.

Convenient Packaging Design

• Individually wrapped in lint free paper / plastic blister
• Color coded, convenient stackable TPP dispenser box
• Single dispense or convenient opening for dispensing larger number of pipettes

Quality Assurance for all TPP Serologicals:

• Manufactured with the highest grade of polystyrene for greater break resistance
• DNAse/RNAse-free, low endotoxin level <0.1EU/ml
• Sterilized by gamma irradiation
• Individually wrapped in peel-off paper/plastic wrapper

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TPP's Packed Cell Vol (PCV) tube enables a quick and reproducible method of cell counting based on cell Vol. A quick centrifuge of the cell suspension pipetted into a PCV tube reveals a packed cell pellet in the PCV capillary, which can be easily read with the aid of an Easy read ruler. This non-subjective method is superior to old-fashioned and labor intensive hemacytometer based counting.

Functional Features:

• Cell suspension sample can be directly quantitated without dilution
• Multiple samples can be assayed simultaneously
• Load and spin as many PCV tubes as your centrifuge rotor holds
• Quick and reproducible
• Cells can be spun down in 1 minute at 2500xg

Ergonomic Features:

• Reproducible measurements
• Graduated capillary model offers immediate quantitation
• For finer quantitation: The Easy Read Ruler  is used with non-graduated PCV tubes
• Eliminates expensive automated counters / fragile slides
• Avoids subjective microscope counting

Quality Assurance:

• Gamma-sterilized, DNAse/RNAse-Free, and pyrogen-Free
• Individually wrapped in peel-off plastic wrapper

Protocol Example:

• Uniformly mix cell suspension collected from TPP  flask/dish/bioreactor
• Pipet 1000µL of cell suspension into a PCV tube
• Spin multiple samples for 1 minute at 2500xg in a standard microcentrifuge
• Record pellet Vol and compare to pre-generated standard curve of cell numbers to packed cell Vol.
• Standard curve needs to be set up only once per cell line
• Pipet pre-determined cell suspension Vols (dilutions) into a hemacytometer
• Calculate and record the number of live cells for each concentration
• Pipet the identical Vols into the PCV tube, centrifuge and record the numeric in the capillary that corresponds to pellet Vol
• Plot the hemacytometer readings vs. the PCV readings for each Vol
• This Standard Curve doesn't need to be redone for the same cell line and moving forward the cell suspension harvested from TPP flasks/dishes/plates only need to be centrifuged in PCV tubes and assayed for cell pellet measurements

High density of cells

• With a very high density of the cells, the samples must not be diluted. You might simply reduce the sample Vol. Samples from the same series should be treated accordingly. 
• For bacteria measurement the parameters have to be adjusted, for example higher g-force, smaller Vol, etc.
• Micro centrifuges with swing-out rotors are preferable.

 

Order#	Description	Quantity
TP87005	PCV Cell Counting Tubes (with capillary graduations only)	50/pk
TP87007	PCV Cell Counting Tubes (no graduations)	50/pk
TP87008	TPP Cap for PCV Tubes	50/pk
TP87010	Easy Read Ruler	1

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TPP Tube Racks

TPP Cryo Box

• Intended for storage of samples in a freezer
• Easy "one-handed" use lids
• Polypropylene
• 133 x 133 x 45 mm

TPP Turbo Fix, USA/Japan
 

The TPP Turbo Fix Pipet-Aid provides significant increase in pipetting speed improving productivity and delivering an enhanced pipetting experience. The Turbo Fix is especially great with our high Vol 40 and 70 mL serologicals.

The Turbo Fix combines all essential characteristics: precision, speed, ease-of-use, comfort, and reliability.

Features of the Turbo-Fix

• Versatile usage: fits all commercially available glass and plastic serological pipettes in the Vol range 1-100 ml
• Precise handling: two operating buttons for sensitive control of the liquid
• Speed control: an adjustment thumb wheel for setting the pipetting speed
• Gravity dispensing: dispensing drop-by-drop by applying a slight pressure on the dispense button
• Turbo-speed: fastest pipettor on the market! The turbo mode provides the instrument with 25% extra pipetting speed. This is important when working with large Vol pipettes with reservoirs. Includes an adjustment thumb wheel for setting the pipetting speed.
• Features of Turbo-Fix pipette aid: Turbo suction force approx. 13.5 ml/sec (with a serological pipette 50 ml)  Lithium-ion polymer rechargeable battery-technology
• Long operating battery life: operating time of over 6.5 hours powered by a high-performance Lithium Polymer battery, 7-10 mV, min. 550 mAh
  
• Ergonomics: The Turbo-Fix is light weight (195g) and well balanced for comfortable pipetting
• Control: stable and exact fitting of the pipettes with the reservoir results in controlled and drop free handling
• Filter protection: an easy to replace hydrophobic filter protects the Turbo-Fix of accidentally absorbing liquids
• Autoclavable pipette nose Stabifix included.
• Power requirements:
  
• • Input: 100-240 VAC, 50/60Hz
  • Output: 17 VDC±10%/180mA±10%
• Operating conditions: 5-40°C, max. 80% RH

Diamond® Essentials™ PP Class B Graduated Measuring Cylinders

Small on the outside, the Sterilmatic has as much inside capacity as many large, more costly conventional sterilizers and up to five times the capacity of smaller table top units. Two models are available, each with roomy 16 dia. x 26 in. D chamber.

Economical, reliable, and simple to operate, the versatile Sterilmatic provides the highest standards of sterilizing performance in hospitals, clinics, and laboratories around the world.

Large Capacity: The big Sterilmatic chamber holds three trays of 12" x 20" x 2.5" size, or two bedpans. Fits on a 24" counter, or may be ordered with a stand.

Versatile: For all requirements of safe and positive saturated steam sterilization: wrapped and unwrapped dressings, instruments, utensils, glassware, gloves, syringes, bedpans, discharge kits, and liquids. Exhaust speed dial with fast (1 minute for instruments and glassware) and slow (10 minutes for liquids) settings. 0 -to - 60-minute timer. Fixed-temperature model heats to 121 °C (250 °F). Variable-temperature model heats from 110 °C to 121 °C (230 °F to 250 °F).

Safe And Simplified Door Design: Self-sealing and self-aligning one-piece internal door can't be opened until steam pressure is completely exhausted. Cumbersome radial arm construction is eliminated. The door locks itself automatically and positively. All controls are front mounted. Sterilizer features a 0-207kPa (0 - 30psi) pressure gauge, temperature gauge, low water cut-off, safety valve, thermostatic steam trap, and signal light.

Easy To Operate: Fill reservoir with water, load, close door, select exhaust cycle, and set time. Sterilizing cycle begins automatically when unit reaches temperature and pressure. Signal light stays on during sterilizing cycle. Unit shuts off and exhausts steam automatically.

Faster Preheat: Sterilmatic reaches sterilizing temperature in 8-10 minutes from a cold start and in 3-7 minutes when in continued use. Conventional counter autoclaves take three times as long. Sterilmatic provides twice as many sterilizing cycles, increasing daily output substantially.

Optional Accessories

• Stainless Steel Stand
• Tray, Perforated Stainless Steel, 30.5W x 50.8L x 6.4D cm(12 x 20 x 2-1/2")
• Tray, Perforated Stainless Steel, 30.5W x 50.8L x 10.2D cm(12 x 20 x 4")
• Tray, Perforated Stainless Steel, 30.5W x 50.8L x 15.2D cm(12 x 20 x 6")
• Basket, Perforated Stainless Steel, 30.5W x 50.8L x 6.4D cm(12 x 20 x 2-1/2")
• Basket, Perforated Stainless Steel, 30.5W x 50.8L x 15.2D cm(12 x 20 x 6")
• Recording Thermometer
• Exhaust Condenser>
• Recording Chart, 24-hour

Warranty: One Year On Parts, 90 Days On Labor

Overall Dimesnions: 48W x 79D x 74Hcm (18-3/4 x 31 x 29")

Chamber Dimensions: 66L x 41 dia. cm (26 x 16")

Chamber Capacity: 3 cu. ft.

Operating Pressures: 15 psi (1 ATM, 100 kPa)

Net Weight: 63 kg (140 lbs)

Required Connections: Drain, 13mm (1/2") FPT or 16mm (5/8") O.D.; copper steam exhaust connection, 10mm (3/8") IPS. For 208/240V 60Hz, 1 or 3 phase; 30 for 240V, 4-wire; 45 for 208V, single phase; 51 for 240V, 3-wire. Must be hardwired in the location of use. All models available for operation on single or three-phase, three or four wire system.

Meets UL and CSA codes. ASME approved.