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IBI Genomic DNA Kits (Cultured Cell and Blood)

Item #: ASDNARNAKIT16 

The Genomic DNA Kit for Blood or Cultured Cells provides an efficient method for purifying total DNA (including genomic, mitochondrial and viral DNA) from whole blood, frozen blood, buffy coat, cultured animal/bacterial cells and fungus. Chaotropic salt is used to lyse cells and degrade protein, allowing DNA to bind to the glass fiber matrix of the spin column. Contaminants are removed using a Wash Buffer (containing ethanol) and the purified genomic DNA is eluted by a low salt Elution Buffer or TE. The entire procedure can be completed in 1 hour without phenol/chloroform extraction or alcohol precipitation, with an average DNA yield of 6 µ g from 200 µ l of whole human blood and up to 50µ g of DNA from 200µ l of buffy coat. Purified DNA, with approximately 20-30 Kb, is suitable for use in PCR or other enzymatic reactions.

 

MINI

MAXI

Sample Size:

up to 300µ l fresh whole blood, up to 10 cultured cells

10ml frozen blood, up to 10 cultured cells

Format:

Spin Column

Spin Column

Expectant Yield:

up to 50µ g DNA in 50-200µ l

up to 140µ g DNA in 1-2ml

Operation Time:

40 min or less

60 min or less

PNRNADNA

Source Cell
Cultured Cell
Isolation type
DNA
SKU Number # of Samples Source Cell Isolation type Price Quantity Add to Cart
IB47200 4 Cultured Cell DNA
30.11
Each
IB47201 100 Cultured Cell DNA
240.66
Each
IB47202 300 Cultured Cell DNA
653.60
Each
IBI MINI Genomic DNA Kit,
IBI Genomic DNA Kits (Cultured Cell and Blood)
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