ION Biosciences IPG-2 TMA+ Salt
 
Yellow-green fluorescent, potassium (K+) indicator, membrane
 

impermeable. Lower K+ affinity than IPG-4 and higher affinity than IPG-1
 

ION Potassium Green – 2 (IPG-2) is a yellow-green fluorescent, intracellular potassium (K+) indicator with Ex/Em: 525 nm/545 nm and a high-sensitivity to detect small changes in K+ concentration. IPG-2 has a higher affinity (Kd = 18 mM) than IPG-1 (Kd = 50 mM) and lower affinity than IPG-4 (Kd = 7 mM).
 

IPG-2 TMA+ salt is a membrane impermeable form of IPG-2 that can be used in lipid membrane-free systems, in liposomes, or can be introduced into cells by electroporation, microinjection or other methods

SBFI AM
 

Ratiometric sodium (Na+) indicator, membrane permeable
 

SBFI is a UV-excitable, ratiometric green indicator for intracellular sodium (Na+) measurements. Ex/Em: 340/505 nm can be used to measure Na+-bound SBFI, and Ex/Em: 380/505 nm can be used to detect Na+-free SBFI. It is ~18X more selective for Na+ over K+. Ratiometry is optimal for imaging applications where quantification of intracellular Na+ concentrations is desired, and reduces effects of photobleaching, heterogenous dye loading, and variable cell morphology.

Red fluorescent, dead cell stain.
 

Ethidium homodimer I (EthD-I) is a membrane-impermeable, high-affinity, nucleic acid stain that selectively labels dead cells with compromised plasma membranes.

Yellow-green fluorescent, potassium (K+) indicator, membrane
 

impermeable. Higher K+ affinity than IPG-1 and IPG-2.
 

ION Potassium Green – 4 (IPG-4) is a yellow-green fluorescent, intracellular potassium (K+) indicator with Ex/Em: 525 nm/545 nm and a high-sensitivity to detect small changes in K+ concentration. IPG-4 has the highest affinity (Kd = 7 mM) among IPG analogues – IPG-2 (Kd = 18 mM) and IPG-1 (Kd = 50 mM).
 

IPG-4 TMA+ salt is a membrane impermeable form of IPG-4 that can be used in lipid membrane-free systems, in liposomes, or can be introduced into cells by electroporation, microinjection or other methods. IPG-4 has been successfully used as a sensor for extracellular K+.

Thallos AM
 

Best in class, green fluorescent, thallium (Tl+) indicator, membrane permeable. Works with potassium (K+), sodium (Na+), and monovalent cation channels, and transporters.
 

Thallos is a green fluorescent, intracellular thallium (Tl+) indicator, and has been the gold standard for fluorescence-based potassium (K+) channel HTS for nearly 2 decades. Thallos also delivers outstanding results for a wide variety of monovalent cation (sodium – Na+) channels, transporters, and GPCRs.

ION Biosciences Fura-2 LR K+ Salt
 

Leakage-resistant, ratiometric calcium (Ca²+) indicator, membrane impermeable.

 

UV-excitable, ratiometric green indicator for intracellular calcium (Ca²+) measurements with longer cellular retention compared to Fura-2 AM and similar spectral properties. Ex/Em: 340/505 nm can be used to measure Ca²+-bound Fura-2, and Ex/Em: 380/505 nm can be used to detect Ca²+-free Fura-2. Ratiometry is optimal for imaging applications where quantification of intracellular Ca²+ concentrations is desired, and reduces effects of photobleaching, heterogenous dye loading, and variable cell morphology.
 

Fura-2 LR K+ salt is a membrane impermeable form of Fura-2 LR that can be used in lipid membrane-free systems, in liposomes, or can be introduced into cells by electroporation, microinjection or other methods.

ION Biosciences Brilliant Sodium Flex Kit
 
ION’s Brilliant Sodium Assay is a total assay solution for multi-well plate-based, high-throughput measurements of changes in intracellular Na+ mediated through a wide-variety of plasma membrane and intracellular sodium channels and transporters. In multi-well, plate-based formats, the Brilliant Sodium Assay can be used to discover and characterize the effects of many tens-of-thousands of compounds and environmental factors on effectors of intracellular Na+. ION’s Brilliant Sodium Assay provides all the reagents necessary for use as a wash or no-wash assay with adherent or non-adherent cells. The optional use of a probenecid solution and an extracellular background masking solution (TRS) offers the ultimate in compatibility for cells types which are difficult to load with fluorescent Na+ indicators (e.g. Chinese Hamster Ovary, CHO cells) and when performing assays in complete, serum-containing cell culture medium is desired.
 

ION’s Brilliant Sodium Assay is compatible with fluorescence microscopes, flow cytometers, and plate readers capable of detecting fluorescein or more optimally, yellow fluorescent protein (YFP).

Our red calcium dye, ION Calcium Red-1 (ICR-1), is a red fluorescent indicator (Ex/Em: 580/660 nm) responsive to intracellular Ca²+ dynamics. ICR-1’s spectral properties allows for multiplexing with an indicator of another color or GFP-tagged cells.

When following the recommended protocol, Red Calcium Essentials provides enough reagents to make 100 mL of working solution, enough for ten 96-well plates. The actual number of assays will vary according to optimal dye concentrations for your application.