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Home > Chemicals/Reagents > Fluorescent, Dyes and Assays > Ratiometric Calcium Flux > ION Biosciences Ratiometric Calcium Essentials

ION Biosciences Ratiometric Calcium Essentials

Item #: ASESSRATIOCA

ION Biosciences RATIOMETRIC CALCIUM ESSENTIALS

Ratiometric Calcium Essentials kit provides the necessary reagents for conducting no wash, ratiometric calcium flux assays compatible with plate reader and fluorescence microscopy applications. Individual components are provided to give the user the flexibility needed to customize your assay.

Fura-2 is the most popular UV-excitable, ratiometric green indicator for intracellular calcium (Ca²+) measurements. Ex/Em: 340/505 nm can be used to measure Ca2+-bound Fura-2, and Ex/Em: 380/505 nm can be used to detect Ca²+-free Fura-2.

Ratiometry is not only optimal for imaging applications where quantification of intracellular Ca²+ concentrations is desired, it also reduces effects of photobleaching, heterogenous dye loading, and variable cell morphology.