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Dioxane Free
PR1MA™ IPTG [Isopropyl-β-D-thiogalactopyranoside], Dioxane Free
Common Name: Isopropyl-β-D-thiogalactopyranoside
CAS Number: 367-93-1
Molecular Weight: 238.3
Chemical Formula: C9H18O5S
Solubility: Water, ethanol
Storage Temp: 2-8°C
IPTG is combined with X-GAL to detect lac+ colonies or cells in a
colorimetric assay. Distinguishes recombinants (white) from non-
recombinants (blue) in cloning strategies using vectors such as Lambda-11,
M13mp 18 and 19, pUC18 and 19, pUR222 containing the lacZ gene.
Dioxane-Free
Water Content: <0.49%
Research or further manufacturing use only, not for food or drug use.
- Appearance: White Crystalline Powder
- Assay (HPLC): 98.0 - 101.0 %
- Purity (HPLC): ≥ 99.0 %
- 1,4-Dioxane: Not Detected
- Absorbance @300nm (5% w/v sol in H2O): ≤ 0.13
- Absorbance @400nm (5% w/v solution in H2O): ≤ 0.06
- Identification: IR Concordant with Standard
- Melting Range: 110 - 114 °C
- Moisture Content (KF): ≤ 1.0 %
- pH (5% in water): 5.0 - 7.0
- Solubility: Soluble in Water and Methanol
- Specific Optical Rotation: -34.5 - -28.5 o
- BSE/TSE Free: BSE/TSE Free
- Non Animal Origin: Non Animal Origin
- Country of Origin: Report
Proudly made in the USA! Click here for more made in America products!
PR1MA™ Benzamidine Hydrochloride
CAS Number: 1670-14-0
Molecular Weight: 156.6
Chemical Formula: C7H8N2 • HCl
Solubility: Water
Storage Temp: 2-8°C
Competitive serine protease inhibitor which forms stable complexes with and blocks the active sites of enzymes. Target enzymes include trypsin, hymotrypsin, kallikzein and plasmin.
Shipping Weight: 0.3 lbs
Shipping Dimensions: 3.50 x 3.75 x 3.50
Research or further manufacturing use only, not for food or drug use.
Specifications
- Appearance: White crystalline powder
- Assay (HPLC): ≥ 98.5 %
- Loss on Drying: ≤ 2 %
- Residue on Ignition: ≤ 0.5 %
- Material Source: Chemical Synthesis
- BSE/TSE Free: BSE/TSE Free
- Country of Origin: Report
Proudly made in the USA! Click here for more made in America products!
Item#:
ASBENZAMIDINE
ION Biosciences IPG-4 AM
Yellow-green fluorescent, potassium (K+) indicator, membrane permeable.
Higher K+ affinity than IPG-1 and IPG-2
ION Potassium Green – 4 (IPG-4) is a yellow-green fluorescent, intracellular potassium (K+) indicator with Ex/Em: 525 nm/545 nm and a high-sensitivity to detect small changes in K+ concentration. IPG-4 has the highest affinity (Kd = 7 mM) among IPG analogues – IPG-2 (Kd = 18 mM) and IPG-1 (Kd = 50 mM).Item#:
ASIPG4AM
ION Biosciences BAPTA AM
Non-fluorescent calcium (Ca²+) chelator, membrane permeable.
BAPTA AM is a highly selective, intracellular calcium (Ca²+) chelator that is less sensitive to pH fluctuations than EGTA and EDTA. Can be used to modulate the level of intracellular Ca²+.
Item#:
ASBAPTAAM
SBFI K+ SALT
Ratiometric sodium (Na+) indicator, membrane impermeable.
SBFI is a UV-excitable, ratiometric green indicator for intracellular sodium (Na+) measurements. Ex/Em: 340/505 nm can be used to measure Na+-bound SBFI, and Ex/Em: 380/505 nm can be used to detect Na+-free SBFI. It is ~18X more selective for Na+ over K+. Ratiometry is optimal for imaging applications where quantification of intracellular Na+ concentrations is desired, and reduces effects of photobleaching, heterogenous dye loading, and variable cell morphology.
SBFI K+ salt is a membrane impermeable form of SBFI that can be used in lipid membrane-free systems, in liposomes, or can be introduced into cells by electroporation, microinjection or other methods.
Item#:
ASSBFIKST
ION Biosciences Fluo-4 K+ Salt
Fluo-4 K+ Salt is a membrane impermeable form of Fluo-4 that can be used in lipid membrane-free systems, in liposomes, or can be introduced into cells by electroporation, microinjection or other methods.
Green fluorescent, calcium (Ca²+) indicator, membrane impermeable.
Fluo-4 is the most popular green fluorescent, calcium (Ca²+) indicator. Proven and trusted for decades to measure intracellular Ca²+ flux in high throughput screening and fluorescence microscopy applications.
Fluo-4 K+ Salt is a membrane impermeable form of Fluo-4 that can be used in lipid membrane-free systems, in liposomes, or can be introduced into cells by electroporation, microinjection or other methods.
Item#:
ASFLUO4AMST
ION Vital - Viability Kit
Two-color, fluorescence-based assay to discriminate between live and dead cells.
ION Vital – Viability kit can be used to quantify live and dead cells within eukaryotic cell suspensions or adherent cultures, 3D cultures, organoids, and some non-fixed tissues. Cannot be used for yeast or bacteria. When following our protocol, ION Vital—Viability kit provides enough reagents to make 150 mL of working solution, enough for fifteen 96-well plates or 1,500 flow cytometry samples. The actual number of assays will vary according to optimal dye concentrations for your application.
Item#:
ASAIONVIT
ION Biosciences PBFI K+ Salt
PBFI is a UV-excitable, ratiometric green indicator for intracellular potassium (K+) measurements. Ex/Em: 340/505 nm can be used to measure K+-bound PBFI, and Ex/Em: 380/505 nm can be used to detect K+-free PBFI. It is 1.5X more selective for K+ over Naand works well in cytosolic conditions where [K+]/[Na] is ~10. Ratiometry is optimal for imaging applications where quantification of intracellular K+ concentrations is desired and reduces effects of photobleaching, heterogenous dye loading, and variable cell morphology.
PBFI is a UV-excitable, ratiometric green indicator for intracellular potassium (K+) measurements. Ex/Em: 340/505 nm can be used to measure K+-bound PBFI, and Ex/Em: 380/505 nm can be used to detect K+-free PBFI. It is 1.5X more selective for K+ over Naand works well in cytosolic conditions where [K+]/[Na] is ~10. Ratiometry is optimal for imaging applications where quantification of intracellular K+ concentrations is desired and reduces effects of photobleaching, heterogenous dye loading, and variable cell morphology.
PBFI K+ salt is a membrane impermeable form of PBFI that can be used in lipid membrane-free systems, in liposomes, or can be introduced into cells by electroporation, microinjection, or other methods.
Item#:
ASPBFIKST
ION Biosciences IPG-1 TMA Salt+
Yellow-green fluorescent, potassium (K+) indicator, membrane
impermeable. Lower K+ affinity than IPG-2 and IPG-4.
ION Potassium Green – 1 (IPG-1) is the best in class yellow-green fluorescent, intracellular potassium (K+) indicator for measuring changes at most intracellular K+ concentrations. IPG-1 has Ex/Em: 525 nm/545 nm and a high-sensitivity to detect small changes in K+ concentration. IPG-1 has a lower affinity (Kd = 50 mM) than IPG-2 (Kd = 18 mM) or IPG-4 (Kd = 7 mM).
IPG-1 TMA+ salt is a membrane impermeable form of IPG-1 that can be used in lipid membrane-free systems, in liposomes, or can be introduced into cells by electroporation, microinjection or other methods.
Item#:
ASIPG1TMAST
ION Biosciences Fluo-2 AM
Green fluorescent, calcium (Ca²+) indicator, membrane permeable. Higher Ca²+ affinity than Fluo-4.
Fluo-2 is a green fluorescent, intracellular calcium (Ca²+) indicator. Fluo-2 has higher Ca²+ affinity (Kd = 290 nM), superior cell loading and comparable spectral properties compared to Fluo-4. Offers superior sensitivity versus other Fluo analogues at lower Ca²+ concentrations.
Brilliant Sodium Assays
ION’s Brilliant Sodium Assay is a total assay solution for multi-well plate-based, high-throughput measurements of changes in intracellular Na+ mediated through a wide-variety of plasma membrane and intracellular sodium channels and transporters. In multi-well, plate-based formats, the Brilliant Sodium Assay can be used to discover and characterize the effects of many tens-of-thousands of compounds and environmental factors on effectors of intracellular Na+. ION’s Brilliant Sodium Assay provides all the reagents necessary for use as a wash or no-wash assay with adherent or non-adherent cells. The optional use of a probenecid solution and an extracellular background masking solution (TRS) offers the ultimate in compatibility for cells types which are difficult to load with fluorescent Na+ indicators (e.g. Chinese Hamster Ovary, CHO cells) and when performing assays in complete, serum-containing cell culture medium is desired.
ION’s Brilliant Sodium Assay is compatible with fluorescence microscopes, flow cytometers, and plate readers capable of detecting fluorescein or more optimally, yellow fluorescent protein (YFP).
Item#:
ASBRTHAS
ION Biosciences PBFI AM
PBFI is a UV-excitable, green ratiometric indicator for intracellular potassium (K) measurements. Ex/Em: 340/505 nm can be used to measure K-bound PBFI, and Ex/Em: 380/505 nm can be used to detect K-free PBFI. It is 1.5X more selective for K over Na, and works well in cytosolic conditions where [K]/[Na] is ~10. Ratiometry is optimal for imaging applications where quantification of intracellular K concentrations is desired, and reduces effects of photobleaching, heterogenous dye loading, and variable cell morphology.
PBFI is a UV-excitable, green ratiometric indicator for intracellular potassium (K) measurements. Ex/Em: 340/505 nm can be used to measure K-bound PBFI, and Ex/Em: 380/505 nm can be used to detect K-free PBFI. It is 1.5X more selective for K over Na, and works well in cytosolic conditions where [K]/[Na] is ~10. Ratiometry is optimal for imaging applications where quantification of intracellular K concentrations is desired, and reduces effects of photobleaching, heterogenous dye loading, and variable cell morphology.
Item#:
ASPBFIAM
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